Restriction map for pF1KSDA0205
 (Linear) (Six-base) MAPSORT of: KIAA0205.seq  Check: 2502  from: 1  to: 1110



KIAA0205.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112    1110 
   Size:        112     998
 
AflIII A'CryG_T

Cuts at:      0     840    1110 
   Size:        840     270
 
AlwNI CAG_nnn'CTG

Cuts at:      0     480    1110 
   Size:        480     630
 
ApaLI G'TGCA_C

Cuts at:      0     321    1110 
   Size:        321     789
 
BamHI G'GATC_C

Cuts at:      0     809    1110 
   Size:        809     301
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     938    1110 
   Size:        938     172
 
BbvCI CC'TCA_GC

Cuts at:      0    1015    1110 
   Size:       1015      95
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     811    1110 
   Size:        811     299
 
BglII A'GATC_T

Cuts at:      0     457    1110 
   Size:        457     653
 
Bme1580I G_kGCm'C

Cuts at:      0     325    1110 
   Size:        325     785
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325     730     901    1110 
   Size:        325     405     171     209

  Fragments arranged by size: 

                 405     325     209     171
 
Bpu10I CC'TnA_GC

Cuts at:      0    1015    1110 
   Size:       1015      95
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     472    1110 
   Size:        472     638
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     722    1110 
   Size:        722     388
 
BseYI C'CCAG_C

Cuts at:      0      30    1110 
   Size:         30    1080
 
BsiHKAI G_wGCw'C

Cuts at:      0     325    1110 
   Size:        325     785
 
BsmI GAATG_Cn'

Cuts at:      0     672    1110 
   Size:        672     438
 
Bsp1286I G_dGCh'C

Cuts at:      0     325    1110 
   Size:        325     785
 
BsrDI GCAATG_nn'

Cuts at:      0    1098    1110 
   Size:       1098      12
 
BsrGI T'GTAC_A

Cuts at:      0     843    1110 
   Size:        843     267
 
BstYI r'GATC_y

Cuts at:      0     457     809     854    1110 
   Size:        457     352      45     256

  Fragments arranged by size: 

                 457     352     256      45
 
Bsu36I CC'TnA_GG

Cuts at:      0     562    1110 
   Size:        562     548
 
BtgI C'CryG_G

Cuts at:      0      25    1110 
   Size:         25    1085
 
BtsI GCAGTG_nn'

Cuts at:      0     290    1110 
   Size:        290     820
 
EarI CTCTTCn'nnn_

Cuts at:      0     562    1110 
   Size:        562     548
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     325     730     901    1110 
   Size:        112     213     405     171     209

  Fragments arranged by size: 

                 405     213     209     171     112
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     513    1110 
   Size:        513     597
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     507     539     718     750     759     791     925     957 
   Size:        507      32     179      32       9      32     134      32

Cuts at:    957    1110 
   Size:        153

  Fragments arranged by size: 

                 507     179     153     134      32      32      32      32
                   9
 
HincII GTy'rAC

Cuts at:      0      81    1110 
   Size:         81    1029
 
MslI CAynn'nnrTG

Cuts at:      0     328     950    1110 
   Size:        328     622     160

  Fragments arranged by size: 

                 622     328     160
 
MspA1I CmG'CkG

Cuts at:      0     477     915    1110 
   Size:        477     438     195

  Fragments arranged by size: 

                 477     438     195
 
NspI r_CATG'y

Cuts at:      0     844    1110 
   Size:        844     266
 
PciI A'CATG_T

Cuts at:      0     840    1110 
   Size:        840     270
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     643    1110 
   Size:        643     467
 
PfoI T'CCnGG_A

Cuts at:      0     341    1110 
   Size:        341     769
 
PsiI TTA'TAA

Cuts at:      0     445    1110 
   Size:        445     665
 
PvuII CAG'CTG

Cuts at:      0     477    1110 
   Size:        477     633
 
SexAI A'CCwGG_T

Cuts at:      0      86    1110 
   Size:         86    1024
 
SfcI C'TryA_G

Cuts at:      0     791    1110 
   Size:        791     319
 
SmlI C'TyrA_G

Cuts at:      0     487    1110 
   Size:        487     623
 
StuI AGG'CCT

Cuts at:      0     743    1110 
   Size:        743     367
 
StyI C'CwwG_G

Cuts at:      0     637     813     977    1110 
   Size:        637     176     164     133

  Fragments arranged by size: 

                 637     176     164     133
 
TatI w'GTAC_w

Cuts at:      0     398     843    1110 
   Size:        398     445     267

  Fragments arranged by size: 

                 445     398     267
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     221     253    1110 
   Size:        221      32     857

  Fragments arranged by size: 

                 857     221      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     417    1110 
   Size:        417     693
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI    ApaLI    BamHI     BbsI    BbvCI    BciVI
    BglII Bme1580I     BpmI   Bpu10I    BpuEI    BseRI    BseYI  BsiHKAI
     BsmI Bsp1286I    BsrDI    BsrGI    BstYI   Bsu36I     BtgI     BtsI
     EarI  Eco57MI     FalI    Hin4I   HincII     MslI   MspA1I     NspI
     PciI    PflMI     PfoI     PsiI    PvuII    SexAI     SfcI     SmlI
     StuI     StyI     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI     BcgI
     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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