Restriction map for
pF1KA0203
(Linear) (Six-base) MAPSORT of: KIAA0203.seq Check: 8063 from: 1 to: 4773
KIAA0203.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 261 4773
Size: 261 4512
AccI GT'mk_AC
Cuts at: 0 2322 2433 3426 4773
Size: 2322 111 993 1347
Fragments arranged by size:
2322 1347 993 111
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1375 2412 2675 2872 2923 2968 3868 4136
Size: 1375 1037 263 197 51 45 900 268
Cuts at: 4136 4379 4442 4613 4773
Size: 243 63 171 160
Fragments arranged by size:
1375 1037 900 268 263 243 197 171
160 63 51 45
AflII C'TTAA_G
Cuts at: 0 82 4773
Size: 82 4691
AflIII A'CryG_T
Cuts at: 0 789 1344 4773
Size: 789 555 3429
Fragments arranged by size:
3429 789 555
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1853 4773
Size: 1853 2920
AlwNI CAG_nnn'CTG
Cuts at: 0 132 814 1752 3211 4773
Size: 132 682 938 1459 1562
Fragments arranged by size:
1562 1459 938 682 132
ApaLI G'TGCA_C
Cuts at: 0 4238 4773
Size: 4238 535
ApoI r'AATT_y
Cuts at: 0 483 2229 2502 2734 3224 3294 3742 4773
Size: 483 1746 273 232 490 70 448 1031
Fragments arranged by size:
1746 1031 490 483 448 273 232 70
AseI AT'TA_AT
Cuts at: 0 3074 4773
Size: 3074 1699
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2327 2360 4773
Size: 2327 33 2413
Fragments arranged by size:
2413 2327 33
BamHI G'GATC_C
Cuts at: 0 2031 4773
Size: 2031 2742
BanII G_rGCy'C
Cuts at: 0 3249 4128 4773
Size: 3249 879 645
Fragments arranged by size:
3249 879 645
BbsI GAAGACnn'nnnn_
Cuts at: 0 554 660 686 2456 2885 4448 4650 4773
Size: 554 106 26 1770 429 1563 202 123
Fragments arranged by size:
1770 1563 554 429 202 123 106 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3940 3974 4773
Size: 3940 34 799
Fragments arranged by size:
3940 799 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 192 2328 4773
Size: 192 2136 2445
Fragments arranged by size:
2445 2136 192
BclI T'GATC_A
Cuts at: 0 1386 4773
Size: 1386 3387
BfrBI ATG'CAT
Cuts at: 0 155 1216 4773
Size: 155 1061 3557
Fragments arranged by size:
3557 1061 155
BglI GCCn_nnn'nGGC
Cuts at: 0 4696 4773
Size: 4696 77
BglII A'GATC_T
Cuts at: 0 1899 4773
Size: 1899 2874
BlpI GC'TnA_GC
Cuts at: 0 1491 3363 4773
Size: 1491 1872 1410
Fragments arranged by size:
1872 1491 1410
Bme1580I G_kGCm'C
Cuts at: 0 4242 4773
Size: 4242 531
BmtI G_CTAG'C
Cuts at: 0 1133 4773
Size: 1133 3640
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2027 4773
Size: 2027 2746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 2057 4773
Size: 490 1567 2716
Fragments arranged by size:
2716 1567 490
Bpu10I CC'TnA_GC
Cuts at: 0 1169 4773
Size: 1169 3604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2725 3127 3655 3820 4156 4773
Size: 2725 402 528 165 336 617
Fragments arranged by size:
2725 617 528 402 336 165
BsaI GGTCTCn'nnnn_
Cuts at: 0 3253 4773
Size: 3253 1520
BsaBI GATnn'nnATC
Cuts at: 0 203 428 896 4773
Size: 203 225 468 3877
Fragments arranged by size:
3877 468 225 203
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2681 2711 4232 4262 4502 4532 4773
Size: 2681 30 1521 30 240 30 241
Fragments arranged by size:
2681 1521 241 240 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 161 2000 2722 3857 4773
Size: 161 1839 722 1135 916
Fragments arranged by size:
1839 1135 916 722 161
BseYI C'CCAG_C
Cuts at: 0 190 446 3248 4773
Size: 190 256 2802 1525
Fragments arranged by size:
2802 1525 256 190
BsiHKAI G_wGCw'C
Cuts at: 0 725 3567 4128 4242 4773
Size: 725 2842 561 114 531
Fragments arranged by size:
2842 725 561 531 114
BsmI GAATG_Cn'
Cuts at: 0 157 1042 2333 4473 4773
Size: 157 885 1291 2140 300
Fragments arranged by size:
2140 1291 885 300 157
BsmBI CGTCTCn'nnnn_
Cuts at: 0 691 2243 4773
Size: 691 1552 2530
Fragments arranged by size:
2530 1552 691
Bsp1286I G_dGCh'C
Cuts at: 0 725 3249 3567 4128 4242 4773
Size: 725 2524 318 561 114 531
Fragments arranged by size:
2524 725 561 531 318 114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 261 4614 4773
Size: 261 4353 159
Fragments arranged by size:
4353 261 159
BsrDI GCAATG_nn'
Cuts at: 0 4316 4393 4773
Size: 4316 77 380
Fragments arranged by size:
4316 380 77
BsrGI T'GTAC_A
Cuts at: 0 417 2475 2515 4332 4773
Size: 417 2058 40 1817 441
Fragments arranged by size:
2058 1817 441 417 40
BssSI C'ACGA_G
Cuts at: 0 2861 3165 4773
Size: 2861 304 1608
Fragments arranged by size:
2861 1608 304
BstBI TT'CG_AA
Cuts at: 0 3960 4773
Size: 3960 813
BstXI CCAn_nnnn'nTGG
Cuts at: 0 717 783 4773
Size: 717 66 3990
Fragments arranged by size:
3990 717 66
BstYI r'GATC_y
Cuts at: 0 706 1899 2031 4267 4614 4773
Size: 706 1193 132 2236 347 159
Fragments arranged by size:
2236 1193 706 347 159 132
BstZ17I GTA'TAC
Cuts at: 0 2434 4773
Size: 2434 2339
Bsu36I CC'TnA_GG
Cuts at: 0 1789 4773
Size: 1789 2984
BtsI GCAGTG_nn'
Cuts at: 0 1313 4773
Size: 1313 3460
DraI TTT'AAA
Cuts at: 0 2609 3747 4713 4773
Size: 2609 1138 966 60
Fragments arranged by size:
2609 1138 966 60
DraIII CAC_nnn'GTG
Cuts at: 0 129 4773
Size: 129 4644
EaeI y'GGCC_r
Cuts at: 0 461 596 1137 4773
Size: 461 135 541 3636
Fragments arranged by size:
3636 541 461 135
EarI CTCTTCn'nnn_
Cuts at: 0 165 1186 2651 3278 3599 3914 4290 4773
Size: 165 1021 1465 627 321 315 376 483
Fragments arranged by size:
1465 1021 627 483 376 321 315 165
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1408 4773
Size: 1408 3365
EcoICRI GAG'CTC
Cuts at: 0 4126 4773
Size: 4126 647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 1375 2057 2412 2675 2872 2923 2968
Size: 490 885 682 355 263 197 51 45
Cuts at: 2968 3868 4136 4379 4442 4613 4773
Size: 900 268 243 63 171 160
Fragments arranged by size:
900 885 682 490 355 268 263 243
197 171 160 63 51 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2707 4773
Size: 2707 2066
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 331 2647 3279 3898 4773
Size: 331 2316 632 619 875
Fragments arranged by size:
2316 875 632 619 331
FspI TGC'GCA
Cuts at: 0 4177 4773
Size: 4177 596
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 949 981 1867 1899 2182 2214 4773
Size: 949 32 886 32 283 32 2559
Fragments arranged by size:
2559 949 886 283 32 32 32
HincII GTy'rAC
Cuts at: 0 24 764 4773
Size: 24 740 4009
Fragments arranged by size:
4009 740 24
HindIII A'AGCT_T
Cuts at: 0 1409 3697 3855 3880 4150 4773
Size: 1409 2288 158 25 270 623
Fragments arranged by size:
2288 1409 623 270 158 25
HpaI GTT'AAC
Cuts at: 0 24 764 4773
Size: 24 740 4009
Fragments arranged by size:
4009 740 24
MfeI C'AATT_G
Cuts at: 0 3389 4399 4773
Size: 3389 1010 374
Fragments arranged by size:
3389 1010 374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 2372 4773
Size: 593 1779 2401
Fragments arranged by size:
2401 1779 593
MscI TGG'CCA
Cuts at: 0 463 598 4773
Size: 463 135 4175
Fragments arranged by size:
4175 463 135
MslI CAynn'nnrTG
Cuts at: 0 1829 2511 4773
Size: 1829 682 2262
Fragments arranged by size:
2262 1829 682
MspA1I CmG'CkG
Cuts at: 0 4273 4371 4773
Size: 4273 98 402
Fragments arranged by size:
4273 402 98
NdeI CA'TA_TG
Cuts at: 0 4203 4349 4773
Size: 4203 146 424
Fragments arranged by size:
4203 424 146
NheI G'CTAG_C
Cuts at: 0 1129 4773
Size: 1129 3644
NsiI A_TGCA'T
Cuts at: 0 157 1218 4773
Size: 157 1061 3555
Fragments arranged by size:
3555 1061 157
NspI r_CATG'y
Cuts at: 0 91 793 1348 1834 4455 4773
Size: 91 702 555 486 2621 318
Fragments arranged by size:
2621 702 555 486 318 91
PasI CC'CwG_GG
Cuts at: 0 4649 4773
Size: 4649 124
PciI A'CATG_T
Cuts at: 0 789 1344 4773
Size: 789 555 3429
Fragments arranged by size:
3429 789 555
PflMI CCAn_nnn'nTGG
Cuts at: 0 132 4773
Size: 132 4641
PfoI T'CCnGG_A
Cuts at: 0 3107 4773
Size: 3107 1666
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 707 739 4773
Size: 707 32 4034
Fragments arranged by size:
4034 707 32
PshAI GACnn'nnGTC
Cuts at: 0 4440 4773
Size: 4440 333
PstI C_TGCA'G
Cuts at: 0 165 330 539 585 2049 2780 3539 4773
Size: 165 165 209 46 1464 731 759 1234
Fragments arranged by size:
1464 1234 759 731 209 165 165 46
SacI G_AGCT'C
Cuts at: 0 4128 4773
Size: 4128 645
SapI GCTCTTCn'nnn_
Cuts at: 0 3914 4773
Size: 3914 859
ScaI AGT'ACT
Cuts at: 0 852 4773
Size: 852 3921
SexAI A'CCwGG_T
Cuts at: 0 125 4773
Size: 125 4648
SfcI C'TryA_G
Cuts at: 0 161 183 326 535 581 1536 2045 2776
Size: 161 22 143 209 46 955 509 731
Cuts at: 2776 3535 4211 4773
Size: 759 676 562
Fragments arranged by size:
955 759 731 676 562 509 209 161
143 46 22
SmlI C'TyrA_G
Cuts at: 0 82 2704 3142 3634 3799 4135 4773
Size: 82 2622 438 492 165 336 638
Fragments arranged by size:
2622 638 492 438 336 165 82
SphI G_CATG'C
Cuts at: 0 91 4773
Size: 91 4682
SspI AAT'ATT
Cuts at: 0 4344 4773
Size: 4344 429
StyI C'CwwG_G
Cuts at: 0 441 1009 1960 2945 4773
Size: 441 568 951 985 1828
Fragments arranged by size:
1828 985 951 568 441
SwaI ATTT'AAAT
Cuts at: 0 3747 4773
Size: 3747 1026
TatI w'GTAC_w
Cuts at: 0 417 850 1468 1484 1687 1833 1972 2475
Size: 417 433 618 16 203 146 139 503
Cuts at: 2475 2515 4066 4332 4773
Size: 40 1551 266 441
Fragments arranged by size:
1551 618 503 441 433 417 266 203
146 139 40 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4232 4264 4773
Size: 4232 32 509
Fragments arranged by size:
4232 509 32
XbaI T'CTAG_A
Cuts at: 0 3906 4639 4773
Size: 3906 733 134
Fragments arranged by size:
3906 733 134
XmnI GAAnn'nnTTC
Cuts at: 0 431 2111 3722 3881 4343 4773
Size: 431 1680 1611 159 462 430
Fragments arranged by size:
1680 1611 462 431 430 159
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AlfI AlwNI ApaLI
ApoI AseI BaeI BamHI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BseYI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrGI BssSI
BstBI BstXI BstYI BstZ17I Bsu36I BtsI DraI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI FalI FspI
Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI
MspA1I NdeI NheI NsiI NspI PasI PciI PflMI
PfoI PpiI PshAI PstI SacI SapI ScaI SexAI
SfcI SmlI SphI SspI StyI SwaI TatI TstI
XbaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AleI AloI
ApaI AscI AsiSI AvaI AvrII BanI BbeI BbvCI
BmgBI BmrI BsaAI BsaHI BsaWI BsgI BsiEI BsiWI
BspEI BspHI BsrBI BsrFI BssHII BstAPI BstEII BtgI
BtgZI ClaI CspCI DrdI EagI EcoO109I EcoRI EcoRV
FseI FspAI HaeII KasI KpnI MluI NaeI NarI
NcoI NgoMIV NotI NruI PacI PmeI PmlI PpuMI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI StuI TaqII Tth111I XcmI XhoI
XmaI ZraI