Restriction map for
pF1KA0197
(Linear) (Six-base) MAPSORT of: KIAA0197.seq Check: 4825 from: 1 to: 3945
KIAA0197.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AccI GT'mk_AC
Cuts at: 0 2761 3945
Size: 2761 1184
Acc65I G'GTAC_C
Cuts at: 0 1583 3945
Size: 1583 2362
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 722 1396 1486 2720 2879 3945
Size: 722 674 90 1234 159 1066
Fragments arranged by size:
1234 1066 722 674 159 90
AflII C'TTAA_G
Cuts at: 0 616 3945
Size: 616 3329
AflIII A'CryG_T
Cuts at: 0 2899 3945
Size: 2899 1046
AleI CACnn'nnGTG
Cuts at: 0 3818 3945
Size: 3818 127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2068 3667 3945
Size: 2068 1599 278
Fragments arranged by size:
2068 1599 278
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 596 628 3945
Size: 596 32 3317
Fragments arranged by size:
3317 596 32
AlwNI CAG_nnn'CTG
Cuts at: 0 517 1212 1747 1821 2112 2314 2698 3334
Size: 517 695 535 74 291 202 384 636
Cuts at: 3334 3514 3945
Size: 180 431
Fragments arranged by size:
695 636 535 517 431 384 291 202
180 74
ApaI G_GGCC'C
Cuts at: 0 1119 3945
Size: 1119 2826
ApaLI G'TGCA_C
Cuts at: 0 376 3945
Size: 376 3569
ApoI r'AATT_y
Cuts at: 0 76 286 1350 1435 1458 1471 2716 2963
Size: 76 210 1064 85 23 13 1245 247
Cuts at: 2963 3945
Size: 982
Fragments arranged by size:
1245 1064 982 247 210 85 76 23
13
AvaI C'yCGr_G
Cuts at: 0 1694 3945
Size: 1694 2251
BanI G'GyrC_C
Cuts at: 0 118 1583 2427 3945
Size: 118 1465 844 1518
Fragments arranged by size:
1518 1465 844 118
BanII G_rGCy'C
Cuts at: 0 15 1119 3945
Size: 15 1104 2826
Fragments arranged by size:
2826 1104 15
BbeI G_GCGC'C
Cuts at: 0 122 3945
Size: 122 3823
BbsI GAAGACnn'nnnn_
Cuts at: 0 217 1492 3945
Size: 217 1275 2453
Fragments arranged by size:
2453 1275 217
BclI T'GATC_A
Cuts at: 0 1242 1832 3945
Size: 1242 590 2113
Fragments arranged by size:
2113 1242 590
BfrBI ATG'CAT
Cuts at: 0 975 3644 3945
Size: 975 2669 301
Fragments arranged by size:
2669 975 301
BglI GCCn_nnn'nGGC
Cuts at: 0 3864 3945
Size: 3864 81
BglII A'GATC_T
Cuts at: 0 2091 3438 3945
Size: 2091 1347 507
Fragments arranged by size:
2091 1347 507
BlpI GC'TnA_GC
Cuts at: 0 37 47 556 1976 2168 3485 3945
Size: 37 10 509 1420 192 1317 460
Fragments arranged by size:
1420 1317 509 460 192 37 10
Bme1580I G_kGCm'C
Cuts at: 0 380 1119 3945
Size: 380 739 2826
Fragments arranged by size:
2826 739 380
BmtI G_CTAG'C
Cuts at: 0 3700 3945
Size: 3700 245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 332 889 1386 2290 3377 3463 3802
Size: 239 93 557 497 904 1087 86 339
Cuts at: 3802 3945
Size: 143
Fragments arranged by size:
1087 904 557 497 339 239 143 93
86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3268 3945
Size: 3268 677
BsaAI yAC'GTr
Cuts at: 0 159 3100 3945
Size: 159 2941 845
Fragments arranged by size:
2941 845 159
BsaBI GATnn'nnATC
Cuts at: 0 1231 3945
Size: 1231 2714
BsaHI Gr'CG_yC
Cuts at: 0 119 3945
Size: 119 3826
BsaWI w'CCGG_w
Cuts at: 0 1797 3945
Size: 1797 2148
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 562 592 829 859 2120 2150 2743 2773
Size: 562 30 237 30 1261 30 593 30
Cuts at: 2773 3436 3466 3945
Size: 663 30 479
Fragments arranged by size:
1261 663 593 562 479 237 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 256 1462 3677 3945
Size: 256 1206 2215 268
Fragments arranged by size:
2215 1206 268 256
BseYI C'CCAG_C
Cuts at: 0 2006 2631 3945
Size: 2006 625 1314
Fragments arranged by size:
2006 1314 625
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2064 3343 3359 3945
Size: 2064 1279 16 586
Fragments arranged by size:
2064 1279 586 16
BsiHKAI G_wGCw'C
Cuts at: 0 380 1021 3945
Size: 380 641 2924
Fragments arranged by size:
2924 641 380
BsmI GAATG_Cn'
Cuts at: 0 3215 3394 3945
Size: 3215 179 551
Fragments arranged by size:
3215 551 179
Bsp1286I G_dGCh'C
Cuts at: 0 15 380 1021 1119 3945
Size: 15 365 641 98 2826
Fragments arranged by size:
2826 641 365 98 15
BspEI T'CCGG_A
Cuts at: 0 1797 3945
Size: 1797 2148
BspHI T'CATG_A
Cuts at: 0 3378 3945
Size: 3378 567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1171 3945
Size: 1171 2774
BsrGI T'GTAC_A
Cuts at: 0 2559 3945
Size: 2559 1386
BstAPI GCAn_nnn'nTGC
Cuts at: 0 690 1212 3945
Size: 690 522 2733
Fragments arranged by size:
2733 690 522
BstBI TT'CG_AA
Cuts at: 0 1455 1992 3231 3945
Size: 1455 537 1239 714
Fragments arranged by size:
1455 1239 714 537
BstEII G'GTnAC_C
Cuts at: 0 724 3945
Size: 724 3221
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1909 2014 3780 3945
Size: 1909 105 1766 165
Fragments arranged by size:
1909 1766 165 105
BstYI r'GATC_y
Cuts at: 0 483 1356 2091 3039 3438 3945
Size: 483 873 735 948 399 507
Fragments arranged by size:
948 873 735 507 483 399
BtgI C'CryG_G
Cuts at: 0 110 947 1103 3945
Size: 110 837 156 2842
Fragments arranged by size:
2842 837 156 110
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3870 3945
Size: 3870 75
BtsI GCAGTG_nn'
Cuts at: 0 306 567 3347 3572 3945
Size: 306 261 2780 225 373
Fragments arranged by size:
2780 373 306 261 225
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 92 127 3945
Size: 92 35 3818
Fragments arranged by size:
3818 92 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 3299 3945
Size: 3299 646
EarI CTCTTCn'nnn_
Cuts at: 0 1217 1730 2416 3945
Size: 1217 513 686 1529
Fragments arranged by size:
1529 1217 686 513
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3460 3945
Size: 3460 485
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 332 722 889 1386 1396 1486 2290
Size: 239 93 390 167 497 10 90 804
Cuts at: 2290 2720 2879 3377 3463 3802 3945
Size: 430 159 498 86 339 143
Fragments arranged by size:
804 498 497 430 390 339 239 167
159 143 93 90 86 10
EcoO109I rG'GnC_Cy
Cuts at: 0 573 1116 3945
Size: 573 543 2829
Fragments arranged by size:
2829 573 543
EcoRI G'AATT_C
Cuts at: 0 76 1435 1471 2716 3945
Size: 76 1359 36 1245 1229
Fragments arranged by size:
1359 1245 1229 76 36
EcoRV GAT'ATC
Cuts at: 0 1110 1689 3945
Size: 1110 579 2256
Fragments arranged by size:
2256 1110 579
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3624 3945
Size: 3624 321
HaeII r_GCGC'y
Cuts at: 0 122 3945
Size: 122 3823
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 324 356 2743 2775 3945
Size: 324 32 2387 32 1170
Fragments arranged by size:
2387 1170 324 32 32
HincII GTy'rAC
Cuts at: 0 362 432 3945
Size: 362 70 3513
Fragments arranged by size:
3513 362 70
HindIII A'AGCT_T
Cuts at: 0 1304 3601 3945
Size: 1304 2297 344
Fragments arranged by size:
2297 1304 344
HpaI GTT'AAC
Cuts at: 0 362 3945
Size: 362 3583
KasI G'GCGC_C
Cuts at: 0 118 3945
Size: 118 3827
KpnI G_GTAC'C
Cuts at: 0 1587 3945
Size: 1587 2358
MfeI C'AATT_G
Cuts at: 0 1907 2563 3282 3945
Size: 1907 656 719 663
Fragments arranged by size:
1907 719 663 656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2307 3916 3945
Size: 2307 1609 29
Fragments arranged by size:
2307 1609 29
MslI CAynn'nnrTG
Cuts at: 0 1558 3193 3818 3945
Size: 1558 1635 625 127
Fragments arranged by size:
1635 1558 625 127
MspA1I CmG'CkG
Cuts at: 0 2112 3397 3502 3945
Size: 2112 1285 105 443
Fragments arranged by size:
2112 1285 443 105
NarI GG'CG_CC
Cuts at: 0 119 3945
Size: 119 3826
NcoI C'CATG_G
Cuts at: 0 947 3945
Size: 947 2998
NdeI CA'TA_TG
Cuts at: 0 1705 2946 3945
Size: 1705 1241 999
Fragments arranged by size:
1705 1241 999
NheI G'CTAG_C
Cuts at: 0 3696 3945
Size: 3696 249
NsiI A_TGCA'T
Cuts at: 0 977 3646 3945
Size: 977 2669 299
Fragments arranged by size:
2669 977 299
NspI r_CATG'y
Cuts at: 0 867 975 979 2903 3945
Size: 867 108 4 1924 1042
Fragments arranged by size:
1924 1042 867 108 4
PciI A'CATG_T
Cuts at: 0 2899 3945
Size: 2899 1046
PflMI CCAn_nnn'nTGG
Cuts at: 0 3334 3945
Size: 3334 611
PfoI T'CCnGG_A
Cuts at: 0 403 1363 3425 3945
Size: 403 960 2062 520
Fragments arranged by size:
2062 960 520 403
PmlI CAC'GTG
Cuts at: 0 3100 3945
Size: 3100 845
PsiI TTA'TAA
Cuts at: 0 1714 1765 1784 3871 3945
Size: 1714 51 19 2087 74
Fragments arranged by size:
2087 1714 74 51 19
PspOMI G'GGCC_C
Cuts at: 0 1115 3945
Size: 1115 2830
PstI C_TGCA'G
Cuts at: 0 91 1812 2089 2777 3945
Size: 91 1721 277 688 1168
Fragments arranged by size:
1721 1168 688 277 91
PvuII CAG'CTG
Cuts at: 0 2112 3397 3945
Size: 2112 1285 548
Fragments arranged by size:
2112 1285 548
ScaI AGT'ACT
Cuts at: 0 2070 2297 3945
Size: 2070 227 1648
Fragments arranged by size:
2070 1648 227
SexAI A'CCwGG_T
Cuts at: 0 639 3945
Size: 639 3306
SfcI C'TryA_G
Cuts at: 0 87 545 1035 1789 1808 2085 2773 3034
Size: 87 458 490 754 19 277 688 261
Cuts at: 3034 3746 3945
Size: 712 199
Fragments arranged by size:
754 712 688 490 458 277 261 199
87 19
SfoI GGC'GCC
Cuts at: 0 120 3945
Size: 120 3825
SmlI C'TyrA_G
Cuts at: 0 616 3247 3945
Size: 616 2631 698
Fragments arranged by size:
2631 698 616
SphI G_CATG'C
Cuts at: 0 979 3945
Size: 979 2966
SspI AAT'ATT
Cuts at: 0 452 1989 3945
Size: 452 1537 1956
Fragments arranged by size:
1956 1537 452
StyI C'CwwG_G
Cuts at: 0 947 3458 3945
Size: 947 2511 487
Fragments arranged by size:
2511 947 487
TatI w'GTAC_w
Cuts at: 0 2068 2295 2559 3945
Size: 2068 227 264 1386
Fragments arranged by size:
2068 1386 264 227
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 829 861 2120 2152 3436 3468 3945
Size: 829 32 1259 32 1284 32 477
Fragments arranged by size:
1284 1259 829 477 32 32 32
XbaI T'CTAG_A
Cuts at: 0 2529 3945
Size: 2529 1416
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3311 3945
Size: 3311 634
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI
BbsI BclI BfrBI BglI BglII BlpI Bme1580I BmtI
BpmI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrGI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DrdI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MmeI MslI MspA1I NarI NcoI NdeI
NheI NsiI NspI PciI PflMI PfoI PmlI PsiI
PspOMI PstI PvuII ScaI SexAI SfcI SfoI SmlI
SphI SspI StyI TatI TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AhdI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BmgBI
BmrI BplI Bpu10I BsaI BsiEI BsiWI BsmBI BsrBI
BsrDI BsrFI BssHII BssSI BstZ17I Bsu36I ClaI DraI
DraIII EaeI EagI EcoICRI EcoNI FseI FspI FspAI
MluI MscI NaeI NgoMIV NotI NruI PacI PasI
PmeI PpiI PpuMI PshAI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TaqII
Tth111I XhoI XmaI XmnI ZraI