Restriction map for
pF1KA0187
(Linear) (Six-base) MAPSORT of: KIAA0187.seq Check: 2583 from: 1 to: 3846
KIAA0187.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3698 3846
Size: 3698 148
AccI GT'mk_AC
Cuts at: 0 2911 3846
Size: 2911 935
AclI AA'CG_TT
Cuts at: 0 490 3846
Size: 490 3356
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 1373 1772 1790 1802 2797 3693 3759
Size: 604 769 399 18 12 995 896 66
Cuts at: 3759 3763 3846
Size: 4 83
Fragments arranged by size:
995 896 769 604 399 83 66 18
12 4
AfeI AGC'GCT
Cuts at: 0 976 3846
Size: 976 2870
AhdI GACnn_n'nnGTC
Cuts at: 0 3375 3589 3846
Size: 3375 214 257
Fragments arranged by size:
3375 257 214
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3288 3320 3846
Size: 3288 32 526
Fragments arranged by size:
3288 526 32
AlwNI CAG_nnn'CTG
Cuts at: 0 617 1884 3846
Size: 617 1267 1962
Fragments arranged by size:
1962 1267 617
ApaI G_GGCC'C
Cuts at: 0 1103 2953 3846
Size: 1103 1850 893
Fragments arranged by size:
1850 1103 893
ApaLI G'TGCA_C
Cuts at: 0 2371 3846
Size: 2371 1475
ApoI r'AATT_y
Cuts at: 0 887 1926 2593 2686 3104 3163 3779 3846
Size: 887 1039 667 93 418 59 616 67
Fragments arranged by size:
1039 887 667 616 418 93 67 59
AvaI C'yCGr_G
Cuts at: 0 633 2813 3846
Size: 633 2180 1033
Fragments arranged by size:
2180 1033 633
BanI G'GyrC_C
Cuts at: 0 637 1987 3564 3686 3846
Size: 637 1350 1577 122 160
Fragments arranged by size:
1577 1350 637 160 122
BanII G_rGCy'C
Cuts at: 0 239 1103 1534 2336 2953 3231 3846
Size: 239 864 431 802 617 278 615
Fragments arranged by size:
864 802 617 615 431 278 239
BbsI GAAGACnn'nnnn_
Cuts at: 0 196 595 1481 1820 2012 2357 2885 2898
Size: 196 399 886 339 192 345 528 13
Cuts at: 2898 3279 3846
Size: 381 567
Fragments arranged by size:
886 567 528 399 381 345 339 196
192 13
BbvCI CC'TCA_GC
Cuts at: 0 2027 3830 3846
Size: 2027 1803 16
Fragments arranged by size:
2027 1803 16
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 88 122 3846
Size: 88 34 3724
Fragments arranged by size:
3724 88 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3314 3846
Size: 3314 532
BclI T'GATC_A
Cuts at: 0 1422 3846
Size: 1422 2424
BfrBI ATG'CAT
Cuts at: 0 2928 3846
Size: 2928 918
BglI GCCn_nnn'nGGC
Cuts at: 0 1159 3846
Size: 1159 2687
BglII A'GATC_T
Cuts at: 0 441 3846
Size: 441 3405
BlpI GC'TnA_GC
Cuts at: 0 1533 3846
Size: 1533 2313
Bme1580I G_kGCm'C
Cuts at: 0 1103 1992 2375 2953 3846
Size: 1103 889 383 578 893
Fragments arranged by size:
1103 893 889 578 383
BmgBI CAC'GTC
Cuts at: 0 2370 3846
Size: 2370 1476
BmrI ACTGGGnnnn_n'
Cuts at: 0 1894 2241 3328 3846
Size: 1894 347 1087 518
Fragments arranged by size:
1894 1087 518 347
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1548 3846
Size: 1548 2298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 1040 1337 1649 1708 2187 3215 3846
Size: 70 970 297 312 59 479 1028 631
Fragments arranged by size:
1028 970 631 479 312 297 70 59
Bpu10I CC'TnA_GC
Cuts at: 0 8 2027 3830 3846
Size: 8 2019 1803 16
Fragments arranged by size:
2019 1803 16 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1483 1543 1847 1978 2791 3846
Size: 1483 60 304 131 813 1055
Fragments arranged by size:
1483 1055 813 304 131 60
BsaI GGTCTCn'nnnn_
Cuts at: 0 1903 3576 3846
Size: 1903 1673 270
Fragments arranged by size:
1903 1673 270
BsaAI yAC'GTr
Cuts at: 0 2763 3846
Size: 2763 1083
BsaHI Gr'CG_yC
Cuts at: 0 3419 3846
Size: 3419 427
BsaWI w'CCGG_w
Cuts at: 0 830 3846
Size: 830 3016
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 544 574 3846
Size: 544 30 3272
Fragments arranged by size:
3272 544 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 875 1414 1542 1782 2257 2344 2859 3742
Size: 875 539 128 240 475 87 515 883
Cuts at: 3742 3745 3748 3846
Size: 3 3 98
Fragments arranged by size:
883 875 539 515 475 240 128 98
87 3 3
BseYI C'CCAG_C
Cuts at: 0 3318 3846
Size: 3318 528
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1572 1823 2713 2785 3475 3676 3846
Size: 1572 251 890 72 690 201 170
Fragments arranged by size:
1572 890 690 251 201 170 72
BsiEI CG_ry'CG
Cuts at: 0 3590 3846
Size: 3590 256
BsiHKAI G_wGCw'C
Cuts at: 0 289 867 1534 2336 2375 3231 3707 3846
Size: 289 578 667 802 39 856 476 139
Fragments arranged by size:
856 802 667 578 476 289 139 39
BsmI GAATG_Cn'
Cuts at: 0 1283 3389 3846
Size: 1283 2106 457
Fragments arranged by size:
2106 1283 457
BsmBI CGTCTCn'nnnn_
Cuts at: 0 89 3846
Size: 89 3757
Bsp1286I G_dGCh'C
Cuts at: 0 239 289 867 1103 1534 1992 2336 2375
Size: 239 50 578 236 431 458 344 39
Cuts at: 2375 2953 3231 3707 3846
Size: 578 278 476 139
Fragments arranged by size:
578 578 476 458 431 344 278 239
236 139 50 39
BspHI T'CATG_A
Cuts at: 0 3606 3846
Size: 3606 240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3698 3846
Size: 3698 148
BsrDI GCAATG_nn'
Cuts at: 0 1740 2928 3846
Size: 1740 1188 918
Fragments arranged by size:
1740 1188 918
BsrFI r'CCGG_y
Cuts at: 0 3585 3846
Size: 3585 261
BstBI TT'CG_AA
Cuts at: 0 3183 3846
Size: 3183 663
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2724 3846
Size: 2724 1122
BstYI r'GATC_y
Cuts at: 0 81 441 2817 3617 3846
Size: 81 360 2376 800 229
Fragments arranged by size:
2376 800 360 229 81
BstZ17I GTA'TAC
Cuts at: 0 2912 3846
Size: 2912 934
Bsu36I CC'TnA_GG
Cuts at: 0 3463 3760 3846
Size: 3463 297 86
Fragments arranged by size:
3463 297 86
BtgI C'CryG_G
Cuts at: 0 3392 3414 3846
Size: 3392 22 432
Fragments arranged by size:
3392 432 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2770 3846
Size: 2770 1076
BtsI GCAGTG_nn'
Cuts at: 0 1578 1589 1878 3487 3625 3846
Size: 1578 11 289 1609 138 221
Fragments arranged by size:
1609 1578 289 221 138 11
DraI TTT'AAA
Cuts at: 0 983 3846
Size: 983 2863
DraIII CAC_nnn'GTG
Cuts at: 0 1801 3846
Size: 1801 2045
DrdI GACnn_nn'nnGTC
Cuts at: 0 360 3846
Size: 360 3486
EaeI y'GGCC_r
Cuts at: 0 3158 3587 3846
Size: 3158 429 259
Fragments arranged by size:
3158 429 259
EagI C'GGCC_G
Cuts at: 0 3587 3846
Size: 3587 259
EarI CTCTTCn'nnn_
Cuts at: 0 109 959 1391 1574 1748 1823 2123 2479
Size: 109 850 432 183 174 75 300 356
Cuts at: 2479 3778 3846
Size: 1299 68
Fragments arranged by size:
1299 850 432 356 300 183 174 109
75 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3594 3846
Size: 3594 252
EcoICRI GAG'CTC
Cuts at: 0 1532 2334 3229 3846
Size: 1532 802 895 617
Fragments arranged by size:
1532 895 802 617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 604 1040 1337 1373 1649 1708 1772
Size: 70 534 436 297 36 276 59 64
Cuts at: 1772 1790 1802 2187 2797 3215 3693 3759 3763
Size: 18 12 385 610 418 478 66 4
Cuts at: 3763 3846
Size: 83
Fragments arranged by size:
610 534 478 436 418 385 297 276
83 70 66 64 59 36 18 12
4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 171 2037 3846
Size: 171 1866 1809
Fragments arranged by size:
1866 1809 171
EcoO109I rG'GnC_Cy
Cuts at: 0 265 346 1099 2220 2389 2949 2950 3516
Size: 265 81 753 1121 169 560 1 566
Cuts at: 3516 3756 3846
Size: 240 90
Fragments arranged by size:
1121 753 566 560 265 240 169 90
81 1
EcoRI G'AATT_C
Cuts at: 0 3779 3846
Size: 3779 67
EcoRV GAT'ATC
Cuts at: 0 807 3846
Size: 807 3039
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3789 3846
Size: 3789 57
HaeII r_GCGC'y
Cuts at: 0 978 3846
Size: 978 2868
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 252 284 408 440 1333 1365 1407 1439
Size: 252 32 124 32 893 32 42 32
Cuts at: 1439 1860 1892 2807 2839 3361 3393 3575 3607
Size: 421 32 915 32 522 32 182 32
Cuts at: 3607 3846
Size: 239
Fragments arranged by size:
915 893 522 421 252 239 182 124
42 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 332 1062 2154 3846
Size: 332 730 1092 1692
Fragments arranged by size:
1692 1092 730 332
HindIII A'AGCT_T
Cuts at: 0 128 1846 2071 3846
Size: 128 1718 225 1775
Fragments arranged by size:
1775 1718 225 128
MfeI C'AATT_G
Cuts at: 0 3838 3846
Size: 3838 8
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 1264 1346 1448 3846
Size: 258 1006 82 102 2398
Fragments arranged by size:
2398 1006 258 102 82
MscI TGG'CCA
Cuts at: 0 3160 3846
Size: 3160 686
MslI CAynn'nnrTG
Cuts at: 0 412 3846
Size: 412 3434
MspA1I CmG'CkG
Cuts at: 0 1537 1750 2026 2580 3684 3846
Size: 1537 213 276 554 1104 162
Fragments arranged by size:
1537 1104 554 276 213 162
NcoI C'CATG_G
Cuts at: 0 3414 3846
Size: 3414 432
NsiI A_TGCA'T
Cuts at: 0 2930 3846
Size: 2930 916
NspI r_CATG'y
Cuts at: 0 897 2928 3846
Size: 897 2031 918
Fragments arranged by size:
2031 918 897
PflMI CCAn_nnn'nTGG
Cuts at: 0 277 702 1113 3846
Size: 277 425 411 2733
Fragments arranged by size:
2733 425 411 277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3427 3459 3846
Size: 3427 32 387
Fragments arranged by size:
3427 387 32
PpuMI rG'GwC_Cy
Cuts at: 0 265 3756 3846
Size: 265 3491 90
Fragments arranged by size:
3491 265 90
PspOMI G'GGCC_C
Cuts at: 0 1099 2949 3846
Size: 1099 1850 897
Fragments arranged by size:
1850 1099 897
PstI C_TGCA'G
Cuts at: 0 80 1596 3846
Size: 80 1516 2250
Fragments arranged by size:
2250 1516 80
PvuII CAG'CTG
Cuts at: 0 1750 2026 2580 3846
Size: 1750 276 554 1266
Fragments arranged by size:
1750 1266 554 276
SacI G_AGCT'C
Cuts at: 0 1534 2336 3231 3846
Size: 1534 802 895 615
Fragments arranged by size:
1534 895 802 615
SapI GCTCTTCn'nnn_
Cuts at: 0 959 2123 3778 3846
Size: 959 1164 1655 68
Fragments arranged by size:
1655 1164 959 68
SexAI A'CCwGG_T
Cuts at: 0 3373 3846
Size: 3373 473
SfcI C'TryA_G
Cuts at: 0 76 1592 2834 3029 3846
Size: 76 1516 1242 195 817
Fragments arranged by size:
1516 1242 817 195 76
SmaI CCC'GGG
Cuts at: 0 635 3846
Size: 635 3211
SmlI C'TyrA_G
Cuts at: 0 1462 1522 1862 1993 2806 3846
Size: 1462 60 340 131 813 1040
Fragments arranged by size:
1462 1040 813 340 131 60
StuI AGG'CCT
Cuts at: 0 735 2032 3846
Size: 735 1297 1814
Fragments arranged by size:
1814 1297 735
StyI C'CwwG_G
Cuts at: 0 1065 2309 3149 3414 3674 3846
Size: 1065 1244 840 265 260 172
Fragments arranged by size:
1244 1065 840 265 260 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 349 389 565 1120 2716 3349 3846
Size: 349 40 176 555 1596 633 497
Fragments arranged by size:
1596 633 555 497 349 176 40
TatI w'GTAC_w
Cuts at: 0 516 3846
Size: 516 3330
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 544 576 3846
Size: 544 32 3270
Fragments arranged by size:
3270 544 32
Tth111I GACn'n_nGTC
Cuts at: 0 1051 3846
Size: 1051 2795
XbaI T'CTAG_A
Cuts at: 0 2124 3846
Size: 2124 1722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1110 2211 3846
Size: 1110 1101 1635
Fragments arranged by size:
1635 1110 1101
XmaI C'CCGG_G
Cuts at: 0 633 3846
Size: 633 3213
XmnI GAAnn'nnTTC
Cuts at: 0 2675 3772 3846
Size: 2675 1097 74
Fragments arranged by size:
2675 1097 74
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AhdI AloI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
HindIII MfeI MmeI MscI MslI MspA1I NcoI NsiI
NspI PflMI PpiI PpuMI PspOMI PstI PvuII SacI
SapI SexAI SfcI SmaI SmlI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AflII AflIII AgeI AleI AlfI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BmtI BsaBI
BsiWI BspEI BsrBI BsrGI BssHII BssSI BstAPI BstEII
ClaI CspCI FseI FspI FspAI HpaI KasI KpnI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PfoI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI XhoI ZraI