(Linear) (Six-base) MAPSORT of: KIAA0186.seq  Check: 4607  from: 1  to: 588



KIAA0186.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     344     479     588 
   Size:        344     135     109

  Fragments arranged by size: 

                 344     135     109
 
AhdI GACnn_n'nnGTC

Cuts at:      0     565     588 
   Size:        565      23
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      80     588 
   Size:         80     508
 
ApoI r'AATT_y

Cuts at:      0     187     472     588 
   Size:        187     285     116

  Fragments arranged by size: 

                 285     187     116
 
BclI T'GATC_A

Cuts at:      0     554     588 
   Size:        554      34
 
BfrBI ATG'CAT

Cuts at:      0     301     588 
   Size:        301     287
 
BglI GCCn_nnn'nGGC

Cuts at:      0      54     588 
   Size:         54     534
 
BglII A'GATC_T

Cuts at:      0     355     588 
   Size:        355     233
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     391     588 
   Size:        391     197
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     588 
   Size:        118     470
 
BsaWI w'CCGG_w

Cuts at:      0     449     588 
   Size:        449     139
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     115     401     588 
   Size:        115     286     187

  Fragments arranged by size: 

                 286     187     115
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     207     588 
   Size:        207     381
 
BsiHKAI G_wGCw'C

Cuts at:      0     267     578     588 
   Size:        267     311      10

  Fragments arranged by size: 

                 311     267      10
 
Bsp1286I G_dGCh'C

Cuts at:      0     267     578     588 
   Size:        267     311      10

  Fragments arranged by size: 

                 311     267      10
 
BssHII G'CGCG_C

Cuts at:      0      44     588 
   Size:         44     544
 
BstYI r'GATC_y

Cuts at:      0     355     588 
   Size:        355     233
 
BtgI C'CryG_G

Cuts at:      0      17     588 
   Size:         17     571
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      25     588 
   Size:         25     563
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     344     479     588 
   Size:        118     226     135     109

  Fragments arranged by size: 

                 226     135     118     109
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     551     583     588 
   Size:        551      32       5

  Fragments arranged by size: 

                 551      32       5
 
HindIII A'AGCT_T

Cuts at:      0     110     588 
   Size:        110     478
 
MslI CAynn'nnrTG

Cuts at:      0     270     588 
   Size:        270     318
 
MspA1I CmG'CkG

Cuts at:      0     552     588 
   Size:        552      36
 
NcoI C'CATG_G

Cuts at:      0      17     588 
   Size:         17     571
 
NsiI A_TGCA'T

Cuts at:      0     303     588 
   Size:        303     285
 
PfoI T'CCnGG_A

Cuts at:      0     569     588 
   Size:        569      19
 
PsiI TTA'TAA

Cuts at:      0     350     588 
   Size:        350     238
 
PvuII CAG'CTG

Cuts at:      0     552     588 
   Size:        552      36
 
SfcI C'TryA_G

Cuts at:      0     227     588 
   Size:        227     361
 
StyI C'CwwG_G

Cuts at:      0      17     588 
   Size:         17     571
 

 Enzymes that do cut:

     AcuI     AhdI     AlfI     ApoI     BclI    BfrBI     BglI    BglII
     BmrI     BpmI    BsaWI    BseRI     BsgI  BsiHKAI Bsp1286I   BssHII
    BstYI     BtgI    BtgZI  Eco57MI    Hin4I  HindIII     MslI   MspA1I
     NcoI     NsiI     PfoI     PsiI    PvuII     SfcI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BlpI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI