Restriction map for
pF1KSDA0181
(Linear) (Six-base) MAPSORT of: KIAA0181.seq Check: 3960 from: 1 to: 6189
KIAA0181.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1663 1701 6189
Size: 1663 38 4488
Fragments arranged by size:
4488 1663 38
AccI GT'mk_AC
Cuts at: 0 5187 6189
Size: 5187 1002
Acc65I G'GTAC_C
Cuts at: 0 5763 6189
Size: 5763 426
AclI AA'CG_TT
Cuts at: 0 4709 6189
Size: 4709 1480
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2932 3419 3686 4382 4808 5576 6080 6110
Size: 2932 487 267 696 426 768 504 30
Cuts at: 6110 6189
Size: 79
Fragments arranged by size:
2932 768 696 504 487 426 267 79
30
AflIII A'CryG_T
Cuts at: 0 3456 6189
Size: 3456 2733
AhdI GACnn_n'nnGTC
Cuts at: 0 5194 5396 6189
Size: 5194 202 793
Fragments arranged by size:
5194 793 202
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3269 6189
Size: 3269 2920
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3175 3207 6189
Size: 3175 32 2982
Fragments arranged by size:
3175 2982 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2298 2361 4333 4608 6189
Size: 2298 63 1972 275 1581
Fragments arranged by size:
2298 1972 1581 275 63
ApaI G_GGCC'C
Cuts at: 0 1232 1849 1959 2015 2065 2378 3178 5686
Size: 1232 617 110 56 50 313 800 2508
Cuts at: 5686 6189
Size: 503
Fragments arranged by size:
2508 1232 800 617 503 313 110 56
50
ApoI r'AATT_y
Cuts at: 0 468 902 1626 1757 2893 3558 3792 3930
Size: 468 434 724 131 1136 665 234 138
Cuts at: 3930 4041 4197 4284 4431 5119 5474 5904 6189
Size: 111 156 87 147 688 355 430 285
Fragments arranged by size:
1136 724 688 665 468 434 430 355
285 234 156 147 138 131 111 87
AseI AT'TA_AT
Cuts at: 0 3767 6189
Size: 3767 2422
AvaI C'yCGr_G
Cuts at: 0 302 555 1995 2523 5685 6189
Size: 302 253 1440 528 3162 504
Fragments arranged by size:
3162 1440 528 504 302 253
AvrII C'CTAG_G
Cuts at: 0 1521 3784 6189
Size: 1521 2263 2405
Fragments arranged by size:
2405 2263 1521
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 5747 5780 6189
Size: 5747 33 409
Fragments arranged by size:
5747 409 33
BamHI G'GATC_C
Cuts at: 0 326 651 1312 3123 4395 5863 6189
Size: 326 325 661 1811 1272 1468 326
Fragments arranged by size:
1811 1468 1272 661 326 326 325
BanI G'GyrC_C
Cuts at: 0 585 933 1596 2076 2982 5763 6189
Size: 585 348 663 480 906 2781 426
Fragments arranged by size:
2781 906 663 585 480 426 348
BanII G_rGCy'C
Cuts at: 0 269 671 975 1232 1638 1849 1959 2015
Size: 269 402 304 257 406 211 110 56
Cuts at: 2015 2065 2378 2580 2653 3178 3352 4500 5591
Size: 50 313 202 73 525 174 1148 1091
Cuts at: 5591 5686 5777 6008 6189
Size: 95 91 231 181
Fragments arranged by size:
1148 1091 525 406 402 313 304 269
257 231 211 202 181 174 110 95
91 73 56 50
BbeI G_GCGC'C
Cuts at: 0 2080 6189
Size: 2080 4109
BbsI GAAGACnn'nnnn_
Cuts at: 0 35 71 4604 5240 6189
Size: 35 36 4533 636 949
Fragments arranged by size:
4533 949 636 36 35
BbvCI CC'TCA_GC
Cuts at: 0 1640 1816 3029 5331 5697 6189
Size: 1640 176 1213 2302 366 492
Fragments arranged by size:
2302 1640 1213 492 366 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1566 1600 4448 4482 6189
Size: 1566 34 2848 34 1707
Fragments arranged by size:
2848 1707 1566 34 34
BclI T'GATC_A
Cuts at: 0 2829 3810 6189
Size: 2829 981 2379
Fragments arranged by size:
2829 2379 981
BglI GCCn_nnn'nGGC
Cuts at: 0 2106 2373 5681 6189
Size: 2106 267 3308 508
Fragments arranged by size:
3308 2106 508 267
BglII A'GATC_T
Cuts at: 0 2784 4533 6189
Size: 2784 1749 1656
Fragments arranged by size:
2784 1749 1656
Bme1580I G_kGCm'C
Cuts at: 0 218 1009 1232 1849 1889 1959 2015 2065
Size: 218 791 223 617 40 70 56 50
Cuts at: 2065 2378 3178 5686 5692 6189
Size: 313 800 2508 6 497
Fragments arranged by size:
2508 800 791 617 497 313 223 218
70 56 50 40 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 906 2617 3047 4406 5422 6189
Size: 906 1711 430 1359 1016 767
Fragments arranged by size:
1711 1359 1016 906 767 430
BmtI G_CTAG'C
Cuts at: 0 5315 6189
Size: 5315 874
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3405 5796 6189
Size: 3405 2391 393
Fragments arranged by size:
3405 2391 393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 971 989 1054 2867 3974 4514 4535
Size: 929 42 18 65 1813 1107 540 21
Cuts at: 4535 4712 5139 5237 5986 6117 6189
Size: 177 427 98 749 131 72
Fragments arranged by size:
1813 1107 929 749 540 427 177 131
98 72 65 42 21 18
Bpu10I CC'TnA_GC
Cuts at: 0 1086 1640 1816 3029 5200 5331 5697 6189
Size: 1086 554 176 1213 2171 131 366 492
Fragments arranged by size:
2171 1213 1086 554 492 366 176 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1790 3512 5536 6189
Size: 1790 1722 2024 653
Fragments arranged by size:
2024 1790 1722 653
BsaHI Gr'CG_yC
Cuts at: 0 1102 2077 6189
Size: 1102 975 4112
Fragments arranged by size:
4112 1102 975
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1052 1082 3175 3205 5235 5265 5565 5595
Size: 1052 30 2093 30 2030 30 300 30
Cuts at: 5595 6189
Size: 594
Fragments arranged by size:
2093 2030 1052 594 300 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 611 649 974 1193 1278 1287 1685 2951
Size: 611 38 325 219 85 9 398 1266
Cuts at: 2951 4127 5866 6189
Size: 1176 1739 323
Fragments arranged by size:
1739 1266 1176 611 398 325 323 219
85 38 9
BseYI C'CCAG_C
Cuts at: 0 759 773 1744 1838 1958 2054 2303 2377
Size: 759 14 971 94 120 96 249 74
Cuts at: 2377 2393 2808 3542 3568 3688 5643 5707 5884
Size: 16 415 734 26 120 1955 64 177
Cuts at: 5884 6189
Size: 305
Fragments arranged by size:
1955 971 759 734 415 305 249 177
120 120 96 94 74 64 26 16
14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2287 2518 5299 5561 6189
Size: 2287 231 2781 262 628
Fragments arranged by size:
2781 2287 628 262 231
BsiHKAI G_wGCw'C
Cuts at: 0 2580 4500 5727 5777 6189
Size: 2580 1920 1227 50 412
Fragments arranged by size:
2580 1920 1227 412 50
BsmI GAATG_Cn'
Cuts at: 0 1535 2345 2465 3206 3293 6189
Size: 1535 810 120 741 87 2896
Fragments arranged by size:
2896 1535 810 741 120 87
Bsp1286I G_dGCh'C
Cuts at: 0 218 269 671 975 1009 1232 1638 1849
Size: 218 51 402 304 34 223 406 211
Cuts at: 1849 1889 1959 2015 2065 2378 2580 2653 3178
Size: 40 70 56 50 313 202 73 525
Cuts at: 3178 3352 4500 5591 5686 5692 5727 5777 6008
Size: 174 1148 1091 95 6 35 50 231
Cuts at: 6008 6189
Size: 181
Fragments arranged by size:
1148 1091 525 406 402 313 304 231
223 218 211 202 181 174 95 73
70 56 51 50 50 40 35 34
6
BspHI T'CATG_A
Cuts at: 0 3110 6189
Size: 3110 3079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1663 1701 2100 3261 3980 6129 6189
Size: 1663 38 399 1161 719 2149 60
Fragments arranged by size:
2149 1663 1161 719 399 60 38
BsrBI CCG'CTC
Cuts at: 0 318 6189
Size: 318 5871
BsrDI GCAATG_nn'
Cuts at: 0 653 6189
Size: 653 5536
BsrFI r'CCGG_y
Cuts at: 0 2903 5417 6189
Size: 2903 2514 772
Fragments arranged by size:
2903 2514 772
BssSI C'ACGA_G
Cuts at: 0 5207 6189
Size: 5207 982
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1884 2115 2505 4333 6189
Size: 1884 231 390 1828 1856
Fragments arranged by size:
1884 1856 1828 390 231
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1752 2008 2401 4140 5619 6189
Size: 1752 256 393 1739 1479 570
Fragments arranged by size:
1752 1739 1479 570 393 256
BstYI r'GATC_y
Cuts at: 0 326 651 700 1312 2784 3123 4395 4533
Size: 326 325 49 612 1472 339 1272 138
Cuts at: 4533 4605 5863 6189
Size: 72 1258 326
Fragments arranged by size:
1472 1272 1258 612 339 326 326 325
138 72 49
Bsu36I CC'TnA_GG
Cuts at: 0 696 2102 4265 5318 6189
Size: 696 1406 2163 1053 871
Fragments arranged by size:
2163 1406 1053 871 696
BtgI C'CryG_G
Cuts at: 0 455 1091 1719 2544 6189
Size: 455 636 628 825 3645
Fragments arranged by size:
3645 825 636 628 455
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3494 6189
Size: 3494 2695
BtsI GCAGTG_nn'
Cuts at: 0 3228 3417 4597 5690 5935 6006 6189
Size: 3228 189 1180 1093 245 71 183
Fragments arranged by size:
3228 1180 1093 245 189 183 71
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 126 128 161 163 5793 5828 6189
Size: 126 2 33 2 5630 35 361
Fragments arranged by size:
5630 361 126 35 33 2 2
DraI TTT'AAA
Cuts at: 0 3797 6189
Size: 3797 2392
EaeI y'GGCC_r
Cuts at: 0 2365 2905 3579 4142 5469 6189
Size: 2365 540 674 563 1327 720
Fragments arranged by size:
2365 1327 720 674 563 540
EarI CTCTTCn'nnn_
Cuts at: 0 1207 2816 3757 4415 4758 5354 5361 6056
Size: 1207 1609 941 658 343 596 7 695
Cuts at: 6056 6189
Size: 133
Fragments arranged by size:
1609 1207 941 695 658 596 343 133
7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3394 5772 6189
Size: 3394 2378 417
Fragments arranged by size:
3394 2378 417
EcoICRI GAG'CTC
Cuts at: 0 2578 4498 5775 6189
Size: 2578 1920 1277 414
Fragments arranged by size:
2578 1920 1277 414
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 971 989 1054 2867 2932 3419 3686
Size: 929 42 18 65 1813 65 487 267
Cuts at: 3686 3974 4382 4514 4535 4712 4808 5139 5237
Size: 288 408 132 21 177 96 331 98
Cuts at: 5237 5576 5986 6080 6110 6117 6189
Size: 339 410 94 30 7 72
Fragments arranged by size:
1813 929 487 410 408 339 331 288
267 177 132 98 96 94 72 65
65 42 30 21 18 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 5848 6189
Size: 5848 341
EcoO109I rG'GnC_Cy
Cuts at: 0 592 1228 1229 1239 1845 1955 2012 2061
Size: 592 636 1 10 606 110 57 49
Cuts at: 2061 2098 2311 2358 2374 2878 3174 3175 3250
Size: 37 213 47 16 504 296 1 75
Cuts at: 3250 4569 5405 5704 6189
Size: 1319 836 299 485
Fragments arranged by size:
1319 836 636 606 592 504 485 299
296 213 110 75 57 49 47 37
16 10 1 1
EcoRI G'AATT_C
Cuts at: 0 902 1626 1757 3930 4197 4284 6189
Size: 902 724 131 2173 267 87 1905
Fragments arranged by size:
2173 1905 902 724 267 131 87
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1059 4269 6189
Size: 1059 3210 1920
Fragments arranged by size:
3210 1920 1059
HaeII r_GCGC'y
Cuts at: 0 2080 6189
Size: 2080 4109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 318 350 1497 1529 1989 2021
Size: 70 32 216 32 1147 32 460 32
Cuts at: 2021 2085 2117 2946 2978 3115 3147 4915 4947
Size: 64 32 829 32 137 32 1768 32
Cuts at: 4947 5180 5212 5382 5414 5593 5625 6189
Size: 233 32 170 32 179 32 564
Fragments arranged by size:
1768 1147 829 564 460 233 216 179
170 137 70 64 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1338 2664 4477 4877 5188 5366 5483 6189
Size: 1338 1326 1813 400 311 178 117 706
Fragments arranged by size:
1813 1338 1326 706 400 311 178 117
HpaI GTT'AAC
Cuts at: 0 1338 6189
Size: 1338 4851
KasI G'GCGC_C
Cuts at: 0 2076 6189
Size: 2076 4113
KpnI G_GTAC'C
Cuts at: 0 5767 6189
Size: 5767 422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1405 2145 3853 6189
Size: 1405 740 1708 2336
Fragments arranged by size:
2336 1708 1405 740
MscI TGG'CCA
Cuts at: 0 4144 5471 6189
Size: 4144 1327 718
Fragments arranged by size:
4144 1327 718
MslI CAynn'nnrTG
Cuts at: 0 544 1471 1673 4655 6189
Size: 544 927 202 2982 1534
Fragments arranged by size:
2982 1534 927 544 202
MspA1I CmG'CkG
Cuts at: 0 313 777 960 1644 1860 2812 4936 6189
Size: 313 464 183 684 216 952 2124 1253
Fragments arranged by size:
2124 1253 952 684 464 313 216 183
NarI GG'CG_CC
Cuts at: 0 2077 6189
Size: 2077 4112
NcoI C'CATG_G
Cuts at: 0 455 2544 6189
Size: 455 2089 3645
Fragments arranged by size:
3645 2089 455
NheI G'CTAG_C
Cuts at: 0 5311 6189
Size: 5311 878
NspI r_CATG'y
Cuts at: 0 1672 1827 1872 2253 2980 3450 3460 5495
Size: 1672 155 45 381 727 470 10 2035
Cuts at: 5495 6189
Size: 694
Fragments arranged by size:
2035 1672 727 694 470 381 155 45
10
PasI CC'CwG_GG
Cuts at: 0 993 1392 1621 1893 4030 4522 6189
Size: 993 399 229 272 2137 492 1667
Fragments arranged by size:
2137 1667 993 492 399 272 229
PciI A'CATG_T
Cuts at: 0 3456 6189
Size: 3456 2733
PflMI CCAn_nnn'nTGG
Cuts at: 0 1983 2094 3502 4030 6189
Size: 1983 111 1408 528 2159
Fragments arranged by size:
2159 1983 1408 528 111
PfoI T'CCnGG_A
Cuts at: 0 510 6189
Size: 510 5679
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3175 3207 6189
Size: 3175 32 2982
Fragments arranged by size:
3175 2982 32
PpuMI rG'GwC_Cy
Cuts at: 0 1239 2358 3250 4569 5405 5704 6189
Size: 1239 1119 892 1319 836 299 485
Fragments arranged by size:
1319 1239 1119 892 836 485 299
PshAI GACnn'nnGTC
Cuts at: 0 2283 6189
Size: 2283 3906
PspOMI G'GGCC_C
Cuts at: 0 1228 1845 1955 2011 2061 2374 3174 5682
Size: 1228 617 110 56 50 313 800 2508
Cuts at: 5682 6189
Size: 507
Fragments arranged by size:
2508 1228 800 617 507 313 110 56
50
PstI C_TGCA'G
Cuts at: 0 611 782 2691 4221 4604 5040 5942 6189
Size: 611 171 1909 1530 383 436 902 247
Fragments arranged by size:
1909 1530 902 611 436 383 247 171
PvuII CAG'CTG
Cuts at: 0 777 960 1644 1860 2812 4936 6189
Size: 777 183 684 216 952 2124 1253
Fragments arranged by size:
2124 1253 952 777 684 216 183
SacI G_AGCT'C
Cuts at: 0 2580 4500 5777 6189
Size: 2580 1920 1277 412
Fragments arranged by size:
2580 1920 1277 412
SalI G'TCGA_C
Cuts at: 0 5186 6189
Size: 5186 1003
SanDI GG'GwC_CC
Cuts at: 0 1239 6189
Size: 1239 4950
SapI GCTCTTCn'nnn_
Cuts at: 0 4758 6056 6189
Size: 4758 1298 133
Fragments arranged by size:
4758 1298 133
ScaI AGT'ACT
Cuts at: 0 3464 6189
Size: 3464 2725
SexAI A'CCwGG_T
Cuts at: 0 2910 6189
Size: 2910 3279
SfcI C'TryA_G
Cuts at: 0 607 778 2417 2687 4217 4600 5036 5938
Size: 607 171 1639 270 1530 383 436 902
Cuts at: 5938 6189
Size: 251
Fragments arranged by size:
1639 1530 902 607 436 383 270 251
171
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2373 6189
Size: 2373 3816
SfoI GGC'GCC
Cuts at: 0 2078 6189
Size: 2078 4111
SmaI CCC'GGG
Cuts at: 0 304 557 5687 6189
Size: 304 253 5130 502
Fragments arranged by size:
5130 502 304 253
SmlI C'TyrA_G
Cuts at: 0 1805 3527 5515 6189
Size: 1805 1722 1988 674
Fragments arranged by size:
1988 1805 1722 674
SphI G_CATG'C
Cuts at: 0 1672 1872 6189
Size: 1672 200 4317
Fragments arranged by size:
4317 1672 200
SrfI GCCC'GGGC
Cuts at: 0 5687 6189
Size: 5687 502
SspI AAT'ATT
Cuts at: 0 203 3771 6189
Size: 203 3568 2418
Fragments arranged by size:
3568 2418 203
StuI AGG'CCT
Cuts at: 0 1520 3821 5944 6189
Size: 1520 2301 2123 245
Fragments arranged by size:
2301 2123 1520 245
StyI C'CwwG_G
Cuts at: 0 455 1023 1363 1521 2544 3169 3311 3784
Size: 455 568 340 158 1023 625 142 473
Cuts at: 3784 5486 5562 6189
Size: 1702 76 627
Fragments arranged by size:
1702 1023 627 625 568 473 455 340
158 142 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 737 2052 3687 5664 6189
Size: 737 1315 1635 1977 525
Fragments arranged by size:
1977 1635 1315 737 525
TatI w'GTAC_w
Cuts at: 0 252 3462 6189
Size: 252 3210 2727
Fragments arranged by size:
3210 2727 252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 220 252 1152 1184 2249 2281 3410 3442
Size: 220 32 900 32 1065 32 1129 32
Cuts at: 3442 4706 4738 4794 4826 5565 5597 6189
Size: 1264 32 56 32 739 32 592
Fragments arranged by size:
1264 1129 1065 900 739 592 220 56
32 32 32 32 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 144 964 1672 2385 3372 5961 6189
Size: 144 820 708 713 987 2589 228
Fragments arranged by size:
2589 987 820 713 708 228 144
XmaI C'CCGG_G
Cuts at: 0 302 555 5685 6189
Size: 302 253 5130 504
Fragments arranged by size:
5130 504 302 253
XmnI GAAnn'nnTTC
Cuts at: 0 3321 6189
Size: 3321 2868
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlfI
AloI AlwNI ApaI ApoI AseI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI
BglI BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII HpaI KasI KpnI MmeI
MscI MslI MspA1I NarI NcoI NheI NspI PasI
PciI PflMI PfoI PpiI PpuMI PshAI PspOMI PstI
PvuII SacI SalI SanDI SapI ScaI SexAI SfcI
SfiI SfoI SmaI SmlI SphI SrfI SspI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI ApaLI AscI AsiSI
BciVI BfrBI BlpI BmgBI BsaI BsaAI BsaBI BsaWI
BsiEI BsiWI BsmBI BspEI BsrGI BssHII BstBI BstEII
BstZ17I ClaI DraIII DrdI EagI EcoRV FseI FspI
FspAI HindIII MfeI MluI NaeI NdeI NgoMIV NotI
NruI NsiI PacI PmeI PmlI PsiI PspXI PsrI
PvuI RsrII SacII SbfI SgfI SgrAI SnaBI SpeI
SwaI Tth111I XbaI XhoI ZraI