Restriction map for
pF1KA0179
(Linear) (Six-base) MAPSORT of: KIAA0179.seq Check: 4878 from: 1 to: 2220
KIAA0179.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1178 2151 2220
Size: 1178 973 69
Fragments arranged by size:
1178 973 69
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 971 1282 1858 1896 2143 2220
Size: 650 321 311 576 38 247 77
Fragments arranged by size:
650 576 321 311 247 77 38
AflIII A'CryG_T
Cuts at: 0 1269 1433 2220
Size: 1269 164 787
Fragments arranged by size:
1269 787 164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1669 2220
Size: 1669 551
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1815 1847 2220
Size: 1815 32 373
Fragments arranged by size:
1815 373 32
AlwNI CAG_nnn'CTG
Cuts at: 0 45 1809 1851 1954 2220
Size: 45 1764 42 103 266
Fragments arranged by size:
1764 266 103 45 42
ApaI G_GGCC'C
Cuts at: 0 827 1392 1676 2074 2220
Size: 827 565 284 398 146
Fragments arranged by size:
827 565 398 284 146
ApoI r'AATT_y
Cuts at: 0 934 2029 2220
Size: 934 1095 191
Fragments arranged by size:
1095 934 191
AvaI C'yCGr_G
Cuts at: 0 584 1237 1383 1449 2078 2220
Size: 584 653 146 66 629 142
Fragments arranged by size:
653 629 584 146 142 66
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2034 2067 2220
Size: 2034 33 153
Fragments arranged by size:
2034 153 33
BanII G_rGCy'C
Cuts at: 0 167 827 1238 1384 1392 1500 1676 1691
Size: 167 660 411 146 8 108 176 15
Cuts at: 1691 2074 2220
Size: 383 146
Fragments arranged by size:
660 411 383 176 167 146 146 108
15 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 118 585 658 742 1123 2044 2131 2220
Size: 118 467 73 84 381 921 87 89
Fragments arranged by size:
921 467 381 118 89 87 84 73
BbvCI CC'TCA_GC
Cuts at: 0 683 1852 2220
Size: 683 1169 368
Fragments arranged by size:
1169 683 368
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1267 1301 2220
Size: 1267 34 919
Fragments arranged by size:
1267 919 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 717 2220
Size: 717 1503
BglI GCCn_nnn'nGGC
Cuts at: 0 1264 1454 1569 2220
Size: 1264 190 115 651
Fragments arranged by size:
1264 651 190 115
Bme1580I G_kGCm'C
Cuts at: 0 827 1392 1538 1676 2074 2220
Size: 827 565 146 138 398 146
Fragments arranged by size:
827 565 398 146 146 138
BmgBI CAC'GTC
Cuts at: 0 1298 2220
Size: 1298 922
BmrI ACTGGGnnnn_n'
Cuts at: 0 754 1316 2220
Size: 754 562 904
Fragments arranged by size:
904 754 562
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 440 1338 1519 2220
Size: 440 898 181 701
Fragments arranged by size:
898 701 440 181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 1319 1705 1801 1931 2220
Size: 817 502 386 96 130 289
Fragments arranged by size:
817 502 386 289 130 96
Bpu10I CC'TnA_GC
Cuts at: 0 683 1852 2220
Size: 683 1169 368
Fragments arranged by size:
1169 683 368
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 732 2220
Size: 499 233 1488
Fragments arranged by size:
1488 499 233
BsaAI yAC'GTr
Cuts at: 0 1270 2220
Size: 1270 950
BsaBI GATnn'nnATC
Cuts at: 0 509 2220
Size: 509 1711
BsaHI Gr'CG_yC
Cuts at: 0 49 2220
Size: 49 2171
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 391 421 1929 1959 2220
Size: 391 30 1508 30 261
Fragments arranged by size:
1508 391 261 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 700 1327 2220
Size: 700 627 893
Fragments arranged by size:
893 700 627
BseYI C'CCAG_C
Cuts at: 0 38 353 1640 1702 1792 2220
Size: 38 315 1287 62 90 428
Fragments arranged by size:
1287 428 315 90 62 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1220 1307 2220
Size: 1220 87 913
Fragments arranged by size:
1220 913 87
BsiEI CG_ry'CG
Cuts at: 0 23 2220
Size: 23 2197
BsiHKAI G_wGCw'C
Cuts at: 0 167 1691 1769 2220
Size: 167 1524 78 451
Fragments arranged by size:
1524 451 167 78
BsmBI CGTCTCn'nnnn_
Cuts at: 0 628 2220
Size: 628 1592
Bsp1286I G_dGCh'C
Cuts at: 0 167 827 1238 1384 1392 1500 1538 1676
Size: 167 660 411 146 8 108 38 138
Cuts at: 1676 1691 1769 2074 2220
Size: 15 78 305 146
Fragments arranged by size:
660 411 305 167 146 146 138 108
78 38 15 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1178 2151 2220
Size: 1178 973 69
Fragments arranged by size:
1178 973 69
BsrBI CCG'CTC
Cuts at: 0 79 2220
Size: 79 2141
BsrDI GCAATG_nn'
Cuts at: 0 176 1576 2220
Size: 176 1400 644
Fragments arranged by size:
1400 644 176
BsrFI r'CCGG_y
Cuts at: 0 18 1563 2220
Size: 18 1545 657
Fragments arranged by size:
1545 657 18
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1170 2220
Size: 1170 1050
BstBI TT'CG_AA
Cuts at: 0 596 2220
Size: 596 1624
BstEII G'GTnAC_C
Cuts at: 0 670 2220
Size: 670 1550
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2208 2220
Size: 2208 12
BstYI r'GATC_y
Cuts at: 0 26 2220
Size: 26 2194
Bsu36I CC'TnA_GG
Cuts at: 0 319 1466 2220
Size: 319 1147 754
Fragments arranged by size:
1147 754 319
BtgI C'CryG_G
Cuts at: 0 1369 2066 2112 2220
Size: 1369 697 46 108
Fragments arranged by size:
1369 697 108 46
BtsI GCAGTG_nn'
Cuts at: 0 1421 1554 1824 1931 2220
Size: 1421 133 270 107 289
Fragments arranged by size:
1421 289 270 133 107
ClaI AT'CG_AT
Cuts at: 0 524 2220
Size: 524 1696
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1530 1565 1742 1777 2220
Size: 1530 35 177 35 443
Fragments arranged by size:
1530 443 177 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 2118 2220
Size: 2118 102
EaeI y'GGCC_r
Cuts at: 0 20 1274 1366 2162 2220
Size: 20 1254 92 796 58
Fragments arranged by size:
1254 796 92 58 20
EagI C'GGCC_G
Cuts at: 0 20 2220
Size: 20 2200
EarI CTCTTCn'nnn_
Cuts at: 0 155 626 711 786 1106 1372 1911 2220
Size: 155 471 85 75 320 266 539 309
Fragments arranged by size:
539 471 320 309 266 155 85 75
EcoICRI GAG'CTC
Cuts at: 0 165 1689 2220
Size: 165 1524 531
Fragments arranged by size:
1524 531 165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 817 971 1282 1319 1705 1801 1858
Size: 650 167 154 311 37 386 96 57
Cuts at: 1858 1896 1931 2143 2220
Size: 38 35 212 77
Fragments arranged by size:
650 386 311 212 167 154 96 77
57 38 37 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 394 1598 2220
Size: 394 1204 622
Fragments arranged by size:
1204 622 394
EcoO109I rG'GnC_Cy
Cuts at: 0 402 823 824 1388 1471 1481 1591 1603
Size: 402 421 1 564 83 10 110 12
Cuts at: 1603 1672 1951 2071 2220
Size: 69 279 120 149
Fragments arranged by size:
564 421 402 279 149 120 110 83
69 12 10 1
EcoRI G'AATT_C
Cuts at: 0 2029 2220
Size: 2029 191
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 396 2070 2220
Size: 396 1674 150
Fragments arranged by size:
1674 396 150
HaeII r_GCGC'y
Cuts at: 0 915 2220
Size: 915 1305
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 406 438 457 489 567 599 1282 1314
Size: 406 32 19 32 78 32 683 32
Cuts at: 1314 1420 1443 1452 1475 2220
Size: 106 23 9 23 745
Fragments arranged by size:
745 683 406 106 78 32 32 32
32 23 23 19 9
HincII GTy'rAC
Cuts at: 0 201 2220
Size: 201 2019
HpaI GTT'AAC
Cuts at: 0 201 2220
Size: 201 2019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1385 2220
Size: 1385 835
MscI TGG'CCA
Cuts at: 0 2164 2220
Size: 2164 56
MslI CAynn'nnrTG
Cuts at: 0 106 2220
Size: 106 2114
MspA1I CmG'CkG
Cuts at: 0 42 211 2220
Size: 42 169 2009
Fragments arranged by size:
2009 169 42
NaeI GCC'GGC
Cuts at: 0 20 1565 2220
Size: 20 1545 655
Fragments arranged by size:
1545 655 20
NgoMIV G'CCGG_C
Cuts at: 0 18 1563 2220
Size: 18 1545 657
Fragments arranged by size:
1545 657 18
NspI r_CATG'y
Cuts at: 0 1437 2220
Size: 1437 783
PasI CC'CwG_GG
Cuts at: 0 1192 2201 2220
Size: 1192 1009 19
Fragments arranged by size:
1192 1009 19
PciI A'CATG_T
Cuts at: 0 1433 2220
Size: 1433 787
PflMI CCAn_nnn'nTGG
Cuts at: 0 45 567 1809 2220
Size: 45 522 1242 411
Fragments arranged by size:
1242 522 411 45
PfoI T'CCnGG_A
Cuts at: 0 68 866 2220
Size: 68 798 1354
Fragments arranged by size:
1354 798 68
PmlI CAC'GTG
Cuts at: 0 1270 2220
Size: 1270 950
PpuMI rG'GwC_Cy
Cuts at: 0 402 1471 1481 2220
Size: 402 1069 10 739
Fragments arranged by size:
1069 739 402 10
PspOMI G'GGCC_C
Cuts at: 0 823 1388 1672 2070 2220
Size: 823 565 284 398 150
Fragments arranged by size:
823 565 398 284 150
PstI C_TGCA'G
Cuts at: 0 1175 1658 2220
Size: 1175 483 562
Fragments arranged by size:
1175 562 483
SacI G_AGCT'C
Cuts at: 0 167 1691 2220
Size: 167 1524 529
Fragments arranged by size:
1524 529 167
SanDI GG'GwC_CC
Cuts at: 0 1481 2220
Size: 1481 739
SapI GCTCTTCn'nnn_
Cuts at: 0 155 711 1372 1911 2220
Size: 155 556 661 539 309
Fragments arranged by size:
661 556 539 309 155
SfcI C'TryA_G
Cuts at: 0 154 1171 1654 2220
Size: 154 1017 483 566
Fragments arranged by size:
1017 566 483 154
SmlI C'TyrA_G
Cuts at: 0 514 711 2078 2220
Size: 514 197 1367 142
Fragments arranged by size:
1367 514 197 142
StuI AGG'CCT
Cuts at: 0 1243 2220
Size: 1243 977
StyI C'CwwG_G
Cuts at: 0 2188 2220
Size: 2188 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 89 652 1954 2220
Size: 89 563 1302 266
Fragments arranged by size:
1302 563 266 89
TatI w'GTAC_w
Cuts at: 0 98 2220
Size: 98 2122
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 173 205 391 423 1929 1961 2220
Size: 173 32 186 32 1506 32 259
Fragments arranged by size:
1506 259 186 173 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2154 2220
Size: 2154 66
XhoI C'TCGA_G
Cuts at: 0 2078 2220
Size: 2078 142
XmnI GAAnn'nnTTC
Cuts at: 0 149 2220
Size: 149 2071
Enzymes that do cut:
AarI AcuI AflIII AlfI AloI AlwNI ApaI ApoI
AvaI BaeI BanII BbsI BbvCI BcgI BciVI BglI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI CspCI
DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII HpaI MmeI MscI
MslI MspA1I NaeI NgoMIV NspI PasI PciI PflMI
PfoI PmlI PpuMI PspOMI PstI SacI SanDI SapI
SfcI SmlI StuI StyI TaqII TatI TstI XcmI
XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI ApaLI AscI AseI AsiSI AvrII BamHI BanI
BbeI BclI BfrBI BglII BlpI BmtI BsaI BsaWI
BsiWI BsmI BspEI BspHI BsrGI BssHII BssSI BstZ17I
BtgZI DraI DrdI EciI EcoRV FseI FspI FspAI
HindIII KasI KpnI MfeI MluI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XmaI ZraI