Restriction map for
pF1KA0178
(Linear) (Six-base) MAPSORT of: KIAA0178.seq Check: 1966 from: 1 to: 3699
KIAA0178.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AccI GT'mk_AC
Cuts at: 0 51 248 2012 3532 3699
Size: 51 197 1764 1520 167
Fragments arranged by size:
1764 1520 197 167 51
Acc65I G'GTAC_C
Cuts at: 0 623 3699
Size: 623 3076
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 655 808 1783 1996 2503 2638 2989
Size: 554 101 153 975 213 507 135 351
Cuts at: 2989 3034 3329 3699
Size: 45 295 370
Fragments arranged by size:
975 554 507 370 351 295 213 153
135 101 45
AfeI AGC'GCT
Cuts at: 0 2038 3196 3699
Size: 2038 1158 503
Fragments arranged by size:
2038 1158 503
AflIII A'CryG_T
Cuts at: 0 2507 3699
Size: 2507 1192
AgeI A'CCGG_T
Cuts at: 0 620 1127 3699
Size: 620 507 2572
Fragments arranged by size:
2572 620 507
AhdI GACnn_n'nnGTC
Cuts at: 0 3381 3699
Size: 3381 318
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 835 3699
Size: 835 2864
AlwNI CAG_nnn'CTG
Cuts at: 0 633 1176 2631 3027 3699
Size: 633 543 1455 396 672
Fragments arranged by size:
1455 672 633 543 396
ApaI G_GGCC'C
Cuts at: 0 3298 3699
Size: 3298 401
ApoI r'AATT_y
Cuts at: 0 1183 2903 3699
Size: 1183 1720 796
Fragments arranged by size:
1720 1183 796
AseI AT'TA_AT
Cuts at: 0 2243 3699
Size: 2243 1456
AvaI C'yCGr_G
Cuts at: 0 2692 3699
Size: 2692 1007
BanI G'GyrC_C
Cuts at: 0 623 2698 2833 3699
Size: 623 2075 135 866
Fragments arranged by size:
2075 866 623 135
BanII G_rGCy'C
Cuts at: 0 204 657 3298 3317 3699
Size: 204 453 2641 19 382
Fragments arranged by size:
2641 453 382 204 19
BbeI G_GCGC'C
Cuts at: 0 2702 3699
Size: 2702 997
BbsI GAAGACnn'nnnn_
Cuts at: 0 1657 2446 3403 3699
Size: 1657 789 957 296
Fragments arranged by size:
1657 957 789 296
BbvCI CC'TCA_GC
Cuts at: 0 266 2984 3699
Size: 266 2718 715
Fragments arranged by size:
2718 715 266
BclI T'GATC_A
Cuts at: 0 3632 3699
Size: 3632 67
BglI GCCn_nnn'nGGC
Cuts at: 0 229 3169 3699
Size: 229 2940 530
Fragments arranged by size:
2940 530 229
BglII A'GATC_T
Cuts at: 0 3248 3699
Size: 3248 451
BlpI GC'TnA_GC
Cuts at: 0 2603 3699
Size: 2603 1096
Bme1580I G_kGCm'C
Cuts at: 0 3282 3298 3699
Size: 3282 16 401
Fragments arranged by size:
3282 401 16
BmtI G_CTAG'C
Cuts at: 0 2224 3699
Size: 2224 1475
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 473 879 930 2883 2947 3699
Size: 473 406 51 1953 64 752
Fragments arranged by size:
1953 752 473 406 64 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1027 1198 1333 1747 2230 3699
Size: 1027 171 135 414 483 1469
Fragments arranged by size:
1469 1027 483 414 171 135
Bpu10I CC'TnA_GC
Cuts at: 0 266 656 2984 3614 3699
Size: 266 390 2328 630 85
Fragments arranged by size:
2328 630 390 266 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2119 3550 3699
Size: 2119 1431 149
Fragments arranged by size:
2119 1431 149
BsaI GGTCTCn'nnnn_
Cuts at: 0 1187 1696 2587 2740 3112 3699
Size: 1187 509 891 153 372 587
Fragments arranged by size:
1187 891 587 509 372 153
BsaHI Gr'CG_yC
Cuts at: 0 2699 3699
Size: 2699 1000
BsaWI w'CCGG_w
Cuts at: 0 620 1127 3699
Size: 620 507 2572
Fragments arranged by size:
2572 620 507
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 191 221 1006 1036 3699
Size: 191 30 785 30 2663
Fragments arranged by size:
2663 785 191 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 1117 1396 2062 2689 3586 3699
Size: 271 846 279 666 627 897 113
Fragments arranged by size:
897 846 666 627 279 271 113
BseYI C'CCAG_C
Cuts at: 0 807 1994 3699
Size: 807 1187 1705
Fragments arranged by size:
1705 1187 807
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2122 3699
Size: 2122 1577
BsiEI CG_ry'CG
Cuts at: 0 1470 1551 3699
Size: 1470 81 2148
Fragments arranged by size:
2148 1470 81
BsiHKAI G_wGCw'C
Cuts at: 0 204 3699
Size: 204 3495
BsmI GAATG_Cn'
Cuts at: 0 374 966 2453 3172 3286 3699
Size: 374 592 1487 719 114 413
Fragments arranged by size:
1487 719 592 413 374 114
Bsp1286I G_dGCh'C
Cuts at: 0 204 657 3282 3298 3317 3699
Size: 204 453 2625 16 19 382
Fragments arranged by size:
2625 453 382 204 19 16
BspHI T'CATG_A
Cuts at: 0 2664 3699
Size: 2664 1035
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 173 3699
Size: 173 3526
BsrDI GCAATG_nn'
Cuts at: 0 1848 1880 3699
Size: 1848 32 1819
Fragments arranged by size:
1848 1819 32
BsrFI r'CCGG_y
Cuts at: 0 620 1127 3699
Size: 620 507 2572
Fragments arranged by size:
2572 620 507
BssSI C'ACGA_G
Cuts at: 0 2921 3699
Size: 2921 778
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1272 3045 3699
Size: 1272 1773 654
Fragments arranged by size:
1773 1272 654
BstBI TT'CG_AA
Cuts at: 0 3176 3699
Size: 3176 523
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1611 1638 3699
Size: 1611 27 2061
Fragments arranged by size:
2061 1611 27
BstYI r'GATC_y
Cuts at: 0 1221 2964 3248 3699
Size: 1221 1743 284 451
Fragments arranged by size:
1743 1221 451 284
BtgI C'CryG_G
Cuts at: 0 195 2115 2597 2837 3080 3699
Size: 195 1920 482 240 243 619
Fragments arranged by size:
1920 619 482 243 240 195
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 544 3699
Size: 544 3155
BtsI GCAGTG_nn'
Cuts at: 0 2775 3013 3282 3699
Size: 2775 238 269 417
Fragments arranged by size:
2775 417 269 238
EaeI y'GGCC_r
Cuts at: 0 1394 1548 2633 3699
Size: 1394 154 1085 1066
Fragments arranged by size:
1394 1085 1066 154
EagI C'GGCC_G
Cuts at: 0 1548 3699
Size: 1548 2151
EarI CTCTTCn'nnn_
Cuts at: 0 461 530 605 1061 1280 1343 2327 2852
Size: 461 69 75 456 219 63 984 525
Cuts at: 2852 2984 3305 3699
Size: 132 321 394
Fragments arranged by size:
984 525 461 456 394 321 219 132
75 69 63
EcoICRI GAG'CTC
Cuts at: 0 202 3699
Size: 202 3497
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 655 808 1027 1198 1333 1747 1783
Size: 554 101 153 219 171 135 414 36
Cuts at: 1783 1996 2230 2503 2638 2989 3034 3329 3699
Size: 213 234 273 135 351 45 295 370
Fragments arranged by size:
554 414 370 351 295 273 234 219
213 171 153 135 135 101 45 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1168 3617 3699
Size: 1168 2449 82
Fragments arranged by size:
2449 1168 82
EcoO109I rG'GnC_Cy
Cuts at: 0 186 1821 2928 3258 3295 3699
Size: 186 1635 1107 330 37 404
Fragments arranged by size:
1635 1107 404 330 186 37
EcoRV GAT'ATC
Cuts at: 0 2250 3699
Size: 2250 1449
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2055 2463 3213 3699
Size: 2055 408 750 486
Fragments arranged by size:
2055 750 486 408
FspI TGC'GCA
Cuts at: 0 2359 3699
Size: 2359 1340
HaeII r_GCGC'y
Cuts at: 0 633 1527 1905 1995 2040 2702 3198 3699
Size: 633 894 378 90 45 662 496 501
Fragments arranged by size:
894 662 633 501 496 378 90 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 44 76 1006 1038 1062 1094 2160 2192
Size: 44 32 930 32 24 32 1066 32
Cuts at: 2192 2586 2618 3367 3399 3699
Size: 394 32 749 32 300
Fragments arranged by size:
1066 930 749 394 300 44 32 32
32 32 32 32 24
HincII GTy'rAC
Cuts at: 0 52 3533 3699
Size: 52 3481 166
Fragments arranged by size:
3481 166 52
HindIII A'AGCT_T
Cuts at: 0 679 2752 3699
Size: 679 2073 947
Fragments arranged by size:
2073 947 679
KasI G'GCGC_C
Cuts at: 0 2698 3699
Size: 2698 1001
KpnI G_GTAC'C
Cuts at: 0 627 3699
Size: 627 3072
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 1079 2441 2624 3699
Size: 360 719 1362 183 1075
Fragments arranged by size:
1362 1075 719 360 183
MscI TGG'CCA
Cuts at: 0 1396 2635 3699
Size: 1396 1239 1064
Fragments arranged by size:
1396 1239 1064
MslI CAynn'nnrTG
Cuts at: 0 1636 3699
Size: 1636 2063
MspA1I CmG'CkG
Cuts at: 0 220 666 3699
Size: 220 446 3033
Fragments arranged by size:
3033 446 220
NarI GG'CG_CC
Cuts at: 0 2699 3699
Size: 2699 1000
NcoI C'CATG_G
Cuts at: 0 195 2115 2597 2837 3080 3699
Size: 195 1920 482 240 243 619
Fragments arranged by size:
1920 619 482 243 240 195
NheI G'CTAG_C
Cuts at: 0 2220 3699
Size: 2220 1479
NspI r_CATG'y
Cuts at: 0 2511 3699
Size: 2511 1188
PasI CC'CwG_GG
Cuts at: 0 2871 3699
Size: 2871 828
PciI A'CATG_T
Cuts at: 0 2507 3699
Size: 2507 1192
PflMI CCAn_nnn'nTGG
Cuts at: 0 1176 1440 3699
Size: 1176 264 2259
Fragments arranged by size:
2259 1176 264
PfoI T'CCnGG_A
Cuts at: 0 1754 2366 3113 3461 3699
Size: 1754 612 747 348 238
Fragments arranged by size:
1754 747 612 348 238
PpuMI rG'GwC_Cy
Cuts at: 0 186 3699
Size: 186 3513
PspOMI G'GGCC_C
Cuts at: 0 3294 3699
Size: 3294 405
PstI C_TGCA'G
Cuts at: 0 671 959 1829 2126 2195 3020 3699
Size: 671 288 870 297 69 825 679
Fragments arranged by size:
870 825 679 671 297 288 69
PvuII CAG'CTG
Cuts at: 0 220 666 3699
Size: 220 446 3033
Fragments arranged by size:
3033 446 220
SacI G_AGCT'C
Cuts at: 0 204 3699
Size: 204 3495
SalI G'TCGA_C
Cuts at: 0 50 3531 3699
Size: 50 3481 168
Fragments arranged by size:
3481 168 50
SapI GCTCTTCn'nnn_
Cuts at: 0 1343 3305 3699
Size: 1343 1962 394
Fragments arranged by size:
1962 1343 394
ScaI AGT'ACT
Cuts at: 0 2140 3699
Size: 2140 1559
SexAI A'CCwGG_T
Cuts at: 0 1433 2309 3699
Size: 1433 876 1390
Fragments arranged by size:
1433 1390 876
SfcI C'TryA_G
Cuts at: 0 667 955 1739 1825 2122 2191 2788 3016
Size: 667 288 784 86 297 69 597 228
Cuts at: 3016 3699
Size: 683
Fragments arranged by size:
784 683 667 597 297 288 228 86
69
SfoI GGC'GCC
Cuts at: 0 2700 3699
Size: 2700 999
SmlI C'TyrA_G
Cuts at: 0 2134 3565 3699
Size: 2134 1431 134
Fragments arranged by size:
2134 1431 134
SspI AAT'ATT
Cuts at: 0 573 3699
Size: 573 3126
StuI AGG'CCT
Cuts at: 0 2794 3699
Size: 2794 905
StyI C'CwwG_G
Cuts at: 0 195 902 1604 1982 2115 2597 2837 3080
Size: 195 707 702 378 133 482 240 243
Cuts at: 3080 3482 3584 3699
Size: 402 102 115
Fragments arranged by size:
707 702 482 402 378 243 240 195
133 115 102
TatI w'GTAC_w
Cuts at: 0 311 347 890 2138 2502 3699
Size: 311 36 543 1248 364 1197
Fragments arranged by size:
1248 1197 543 364 311 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 191 223 3699
Size: 191 32 3476
Fragments arranged by size:
3476 191 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2418 3087 3699
Size: 2418 669 612
Fragments arranged by size:
2418 669 612
XmnI GAAnn'nnTTC
Cuts at: 0 3361 3699
Size: 3361 338
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AgeI AhdI AlfI
AlwNI ApaI ApoI AseI AvaI BanI BanII BbeI
BbsI BbvCI BclI BglI BglII BlpI Bme1580I BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MmeI MscI MslI MspA1I NarI
NcoI NheI NspI PasI PciI PflMI PfoI PpuMI
PspOMI PstI PvuII SacI SalI SapI ScaI SexAI
SfcI SfoI SmlI SspI StuI StyI TatI TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AleI AloI ApaLI AscI
AsiSI AvrII BaeI BamHI BcgI BciVI BfrBI BmgBI
BmrI BsaAI BsaBI BsiWI BsmBI BspEI BsrBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EciI EcoRI FseI FspAI HpaI MfeI MluI
NaeI NdeI NgoMIV NotI NruI NsiI PacI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TaqII Tth111I XbaI XhoI
XmaI ZraI