Restriction map for
pF1KA0177
(Linear) (Six-base) MAPSORT of: KIAA0177.seq Check: 5790 from: 1 to: 5172
KIAA0177.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AatII G_ACGT'C
Cuts at: 0 1406 5172
Size: 1406 3766
AccI GT'mk_AC
Cuts at: 0 2597 3334 3608 4734 5172
Size: 2597 737 274 1126 438
Fragments arranged by size:
2597 1126 737 438 274
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 502 644 685 725 1187 1583 2278
Size: 353 149 142 41 40 462 396 695
Cuts at: 2278 3331 3794 5057 5172
Size: 1053 463 1263 115
Fragments arranged by size:
1263 1053 695 463 462 396 353 149
142 115 41 40
AflII C'TTAA_G
Cuts at: 0 3036 5172
Size: 3036 2136
AflIII A'CryG_T
Cuts at: 0 3837 5172
Size: 3837 1335
AhdI GACnn_n'nnGTC
Cuts at: 0 3253 5172
Size: 3253 1919
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1062 5172
Size: 1062 4110
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2198 2230 5172
Size: 2198 32 2942
Fragments arranged by size:
2942 2198 32
AlwNI CAG_nnn'CTG
Cuts at: 0 542 1612 3892 4333 5109 5172
Size: 542 1070 2280 441 776 63
Fragments arranged by size:
2280 1070 776 542 441 63
ApaLI G'TGCA_C
Cuts at: 0 132 3296 4686 5172
Size: 132 3164 1390 486
Fragments arranged by size:
3164 1390 486 132
ApoI r'AATT_y
Cuts at: 0 183 422 762 1171 1639 1687 1770 2386
Size: 183 239 340 409 468 48 83 616
Cuts at: 2386 3316 3725 3822 5172
Size: 930 409 97 1350
Fragments arranged by size:
1350 930 616 468 409 409 340 239
183 97 83 48
AvaI C'yCGr_G
Cuts at: 0 1470 2897 3196 3386 5172
Size: 1470 1427 299 190 1786
Fragments arranged by size:
1786 1470 1427 299 190
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4885 4918 5172
Size: 4885 33 254
Fragments arranged by size:
4885 254 33
BamHI G'GATC_C
Cuts at: 0 4127 5172
Size: 4127 1045
BanI G'GyrC_C
Cuts at: 0 1486 2933 3058 3222 4279 5172
Size: 1486 1447 125 164 1057 893
Fragments arranged by size:
1486 1447 1057 893 164 125
BanII G_rGCy'C
Cuts at: 0 1222 3393 4213 5172
Size: 1222 2171 820 959
Fragments arranged by size:
2171 1222 959 820
BbsI GAAGACnn'nnnn_
Cuts at: 0 386 603 3125 3358 4489 5172
Size: 386 217 2522 233 1131 683
Fragments arranged by size:
2522 1131 683 386 233 217
BbvCI CC'TCA_GC
Cuts at: 0 56 5172
Size: 56 5116
BciVI GTATCCnnnnn_n'
Cuts at: 0 2292 2789 4654 5172
Size: 2292 497 1865 518
Fragments arranged by size:
2292 1865 518 497
BclI T'GATC_A
Cuts at: 0 312 1731 5172
Size: 312 1419 3441
Fragments arranged by size:
3441 1419 312
BfrBI ATG'CAT
Cuts at: 0 4081 5172
Size: 4081 1091
BglI GCCn_nnn'nGGC
Cuts at: 0 3208 5172
Size: 3208 1964
BglII A'GATC_T
Cuts at: 0 4488 5172
Size: 4488 684
Bme1580I G_kGCm'C
Cuts at: 0 136 1955 2224 2936 3197 3300 4690 5172
Size: 136 1819 269 712 261 103 1390 482
Fragments arranged by size:
1819 1390 712 482 269 261 136 103
BmgBI CAC'GTC
Cuts at: 0 3838 5172
Size: 3838 1334
BmrI ACTGGGnnnn_n'
Cuts at: 0 1216 3045 4477 4783 5083 5094 5172
Size: 1216 1829 1432 306 300 11 78
Fragments arranged by size:
1829 1432 1216 306 300 78 11
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4882 5172
Size: 4882 290
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 519 1721 1822 1888 2634 2923 4271 5101
Size: 519 1202 101 66 746 289 1348 830
Cuts at: 5101 5172
Size: 71
Fragments arranged by size:
1348 1202 830 746 519 289 101 71
66
Bpu10I CC'TnA_GC
Cuts at: 0 56 2174 2783 4289 5172
Size: 56 2118 609 1506 883
Fragments arranged by size:
2118 1506 883 609 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 832 5172
Size: 422 410 4340
Fragments arranged by size:
4340 422 410
BsaI GGTCTCn'nnnn_
Cuts at: 0 583 5172
Size: 583 4589
BsaAI yAC'GTr
Cuts at: 0 1950 2379 5172
Size: 1950 429 2793
Fragments arranged by size:
2793 1950 429
BsaBI GATnn'nnATC
Cuts at: 0 10 5172
Size: 10 5162
BsaHI Gr'CG_yC
Cuts at: 0 1403 4141 5172
Size: 1403 2738 1031
Fragments arranged by size:
2738 1403 1031
BsaWI w'CCGG_w
Cuts at: 0 2130 5172
Size: 2130 3042
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 291 321 505 535 554 584 3145 3175
Size: 291 30 184 30 19 30 2561 30
Cuts at: 3175 4143 4173 5172
Size: 968 30 999
Fragments arranged by size:
2561 999 968 291 184 30 30 30
30 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 388 2988 4052 4717 5172
Size: 388 2600 1064 665 455
Fragments arranged by size:
2600 1064 665 455 388
BseYI C'CCAG_C
Cuts at: 0 2066 3213 4304 5109 5172
Size: 2066 1147 1091 805 63
Fragments arranged by size:
2066 1147 1091 805 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3261 4163 4208 5172
Size: 3261 902 45 964
Fragments arranged by size:
3261 964 902 45
BsiEI CG_ry'CG
Cuts at: 0 3965 5172
Size: 3965 1207
BsiHKAI G_wGCw'C
Cuts at: 0 136 3300 3393 4213 4690 5172
Size: 136 3164 93 820 477 482
Fragments arranged by size:
3164 820 482 477 136 93
BsiWI C'GTAC_G
Cuts at: 0 2375 5172
Size: 2375 2797
BsmI GAATG_Cn'
Cuts at: 0 3416 4832 5172
Size: 3416 1416 340
Fragments arranged by size:
3416 1416 340
Bsp1286I G_dGCh'C
Cuts at: 0 136 1222 1955 2224 2936 3197 3300 3393
Size: 136 1086 733 269 712 261 103 93
Cuts at: 3393 4213 4690 5172
Size: 820 477 482
Fragments arranged by size:
1086 820 733 712 482 477 269 261
136 103 93
BspEI T'CCGG_A
Cuts at: 0 2130 5172
Size: 2130 3042
BspHI T'CATG_A
Cuts at: 0 758 2406 3441 5172
Size: 758 1648 1035 1731
Fragments arranged by size:
1731 1648 1035 758
BsrDI GCAATG_nn'
Cuts at: 0 739 3246 5172
Size: 739 2507 1926
Fragments arranged by size:
2507 1926 739
BsrFI r'CCGG_y
Cuts at: 0 1831 5172
Size: 1831 3341
BsrGI T'GTAC_A
Cuts at: 0 1323 3322 5172
Size: 1323 1999 1850
Fragments arranged by size:
1999 1850 1323
BssSI C'ACGA_G
Cuts at: 0 2965 5172
Size: 2965 2207
BstAPI GCAn_nnn'nTGC
Cuts at: 0 741 2432 4231 5172
Size: 741 1691 1799 941
Fragments arranged by size:
1799 1691 941 741
BstBI TT'CG_AA
Cuts at: 0 2009 5172
Size: 2009 3163
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1374 2101 2528 2658 4890 5172
Size: 1374 727 427 130 2232 282
Fragments arranged by size:
2232 1374 727 427 282 130
BstYI r'GATC_y
Cuts at: 0 4127 4488 5172
Size: 4127 361 684
Fragments arranged by size:
4127 684 361
Bsu36I CC'TnA_GG
Cuts at: 0 2168 3795 4423 4451 5172
Size: 2168 1627 628 28 721
Fragments arranged by size:
2168 1627 721 628 28
BtgI C'CryG_G
Cuts at: 0 2100 2453 2657 2804 2840 3919 5062 5172
Size: 2100 353 204 147 36 1079 1143 110
Fragments arranged by size:
2100 1143 1079 353 204 147 110 36
BtsI GCAGTG_nn'
Cuts at: 0 2484 3273 4237 5172
Size: 2484 789 964 935
Fragments arranged by size:
2484 964 935 789
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1608 1643 3506 3541 5172
Size: 1608 35 1863 35 1631
Fragments arranged by size:
1863 1631 1608 35 35
EaeI y'GGCC_r
Cuts at: 0 1115 3688 3962 5172
Size: 1115 2573 274 1210
Fragments arranged by size:
2573 1210 1115 274
EagI C'GGCC_G
Cuts at: 0 3962 5172
Size: 3962 1210
EarI CTCTTCn'nnn_
Cuts at: 0 2965 3779 4325 4664 4851 5147 5172
Size: 2965 814 546 339 187 296 25
Fragments arranged by size:
2965 814 546 339 296 187 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 116 5172
Size: 116 5056
EcoICRI GAG'CTC
Cuts at: 0 3391 4211 5172
Size: 3391 820 961
Fragments arranged by size:
3391 961 820
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 502 519 644 685 725 1187 1583
Size: 353 149 17 125 41 40 462 396
Cuts at: 1583 1721 1822 1888 2278 2634 2923 3331 3794
Size: 138 101 66 390 356 289 408 463
Cuts at: 3794 4271 5057 5101 5172
Size: 477 786 44 71
Fragments arranged by size:
786 477 463 462 408 396 390 356
353 289 149 138 125 101 71 66
44 41 40 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 989 2897 5172
Size: 989 1908 2275
Fragments arranged by size:
2275 1908 989
EcoO109I rG'GnC_Cy
Cuts at: 0 819 1301 3201 3210 4099 4156 5172
Size: 819 482 1900 9 889 57 1016
Fragments arranged by size:
1900 1016 889 819 482 57 9
EcoRI G'AATT_C
Cuts at: 0 183 762 2386 5172
Size: 183 579 1624 2786
Fragments arranged by size:
2786 1624 579 183
EcoRV GAT'ATC
Cuts at: 0 557 2875 5172
Size: 557 2318 2297
Fragments arranged by size:
2318 2297 557
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 129 273 760 2361 2366 2579 5172
Size: 129 144 487 1601 5 213 2593
Fragments arranged by size:
2593 1601 487 213 144 129 5
FseI GG_CCGG'CC
Cuts at: 0 1835 5172
Size: 1835 3337
HaeII r_GCGC'y
Cuts at: 0 2108 5172
Size: 2108 3064
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1484 1516 2474 2506 2622 2654 3239 3271
Size: 1484 32 958 32 116 32 585 32
Cuts at: 3271 3380 3412 3538 3570 4526 4558 4567 4599
Size: 109 32 126 32 956 32 9 32
Cuts at: 4599 5074 5106 5172
Size: 475 32 66
Fragments arranged by size:
1484 958 956 585 475 126 116 109
66 32 32 32 32 32 32 32
32 32 9
HincII GTy'rAC
Cuts at: 0 2598 3335 5172
Size: 2598 737 1837
Fragments arranged by size:
2598 1837 737
HindIII A'AGCT_T
Cuts at: 0 2343 3519 5172
Size: 2343 1176 1653
Fragments arranged by size:
2343 1653 1176
MfeI C'AATT_G
Cuts at: 0 730 946 5172
Size: 730 216 4226
Fragments arranged by size:
4226 730 216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1386 2019 2133 3082 3844 4898 5172
Size: 1386 633 114 949 762 1054 274
Fragments arranged by size:
1386 1054 949 762 633 274 114
MscI TGG'CCA
Cuts at: 0 1117 3690 5172
Size: 1117 2573 1482
Fragments arranged by size:
2573 1482 1117
MslI CAynn'nnrTG
Cuts at: 0 1727 3428 5172
Size: 1727 1701 1744
Fragments arranged by size:
1744 1727 1701
MspA1I CmG'CkG
Cuts at: 0 1996 2806 4057 4604 5172
Size: 1996 810 1251 547 568
Fragments arranged by size:
1996 1251 810 568 547
NaeI GCC'GGC
Cuts at: 0 1833 5172
Size: 1833 3339
NcoI C'CATG_G
Cuts at: 0 2100 2453 2657 2840 3919 5172
Size: 2100 353 204 183 1079 1253
Fragments arranged by size:
2100 1253 1079 353 204 183
NgoMIV G'CCGG_C
Cuts at: 0 1831 5172
Size: 1831 3341
NsiI A_TGCA'T
Cuts at: 0 4083 5172
Size: 4083 1089
NspI r_CATG'y
Cuts at: 0 843 1153 2571 2704 5172
Size: 843 310 1418 133 2468
Fragments arranged by size:
2468 1418 843 310 133
PasI CC'CwG_GG
Cuts at: 0 822 2094 4988 5172
Size: 822 1272 2894 184
Fragments arranged by size:
2894 1272 822 184
PflMI CCAn_nnn'nTGG
Cuts at: 0 3378 5172
Size: 3378 1794
PfoI T'CCnGG_A
Cuts at: 0 572 1838 2939 4248 4471 4868 4975 5172
Size: 572 1266 1101 1309 223 397 107 197
Fragments arranged by size:
1309 1266 1101 572 397 223 197 107
PmlI CAC'GTG
Cuts at: 0 1950 5172
Size: 1950 3222
PpuMI rG'GwC_Cy
Cuts at: 0 4156 5172
Size: 4156 1016
PsiI TTA'TAA
Cuts at: 0 284 2185 5172
Size: 284 1901 2987
Fragments arranged by size:
2987 1901 284
PspXI vC'TCGA_Gb
Cuts at: 0 2897 5172
Size: 2897 2275
PstI C_TGCA'G
Cuts at: 0 3209 5172
Size: 3209 1963
PvuII CAG'CTG
Cuts at: 0 4057 4604 5172
Size: 4057 547 568
Fragments arranged by size:
4057 568 547
SacI G_AGCT'C
Cuts at: 0 3393 4213 5172
Size: 3393 820 959
Fragments arranged by size:
3393 959 820
SacII CC_GC'GG
Cuts at: 0 2807 5172
Size: 2807 2365
SalI G'TCGA_C
Cuts at: 0 2596 3333 5172
Size: 2596 737 1839
Fragments arranged by size:
2596 1839 737
SbfI CC_TGCA'GG
Cuts at: 0 3209 5172
Size: 3209 1963
ScaI AGT'ACT
Cuts at: 0 49 1462 4592 5172
Size: 49 1413 3130 580
Fragments arranged by size:
3130 1413 580 49
SexAI A'CCwGG_T
Cuts at: 0 2989 4912 5172
Size: 2989 1923 260
Fragments arranged by size:
2989 1923 260
SfcI C'TryA_G
Cuts at: 0 1904 3017 3205 4325 4735 4897 5172
Size: 1904 1113 188 1120 410 162 275
Fragments arranged by size:
1904 1120 1113 410 275 188 162
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3208 5172
Size: 3208 1964
SmaI CCC'GGG
Cuts at: 0 1472 5172
Size: 1472 3700
SmlI C'TyrA_G
Cuts at: 0 437 847 2897 3036 5172
Size: 437 410 2050 139 2136
Fragments arranged by size:
2136 2050 437 410 139
SphI G_CATG'C
Cuts at: 0 2571 2704 5172
Size: 2571 133 2468
Fragments arranged by size:
2571 2468 133
SspI AAT'ATT
Cuts at: 0 2739 3076 4983 5172
Size: 2739 337 1907 189
Fragments arranged by size:
2739 1907 337 189
StuI AGG'CCT
Cuts at: 0 4269 5172
Size: 4269 903
StyI C'CwwG_G
Cuts at: 0 467 1413 1826 2100 2453 2657 2684 2711
Size: 467 946 413 274 353 204 27 27
Cuts at: 2711 2840 3919 4095 4714 5172
Size: 129 1079 176 619 458
Fragments arranged by size:
1079 946 619 467 458 413 353 274
204 176 129 27 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2707 5172
Size: 2707 2465
TatI w'GTAC_w
Cuts at: 0 47 1323 1447 1460 3322 3801 4590 5172
Size: 47 1276 124 13 1862 479 789 582
Fragments arranged by size:
1862 1276 789 582 479 124 47 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 291 323 505 537 554 586 2897 2929
Size: 291 32 182 32 17 32 2311 32
Cuts at: 2929 3145 3177 4143 4175 5172
Size: 216 32 966 32 997
Fragments arranged by size:
2311 997 966 291 216 182 32 32
32 32 32 32 17
Tth111I GACn'n_nGTC
Cuts at: 0 2358 3870 5172
Size: 2358 1512 1302
Fragments arranged by size:
2358 1512 1302
XbaI T'CTAG_A
Cuts at: 0 4500 5172
Size: 4500 672
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 227 1846 2838 3744 5106 5172
Size: 227 1619 992 906 1362 66
Fragments arranged by size:
1619 1362 992 906 227 66
XhoI C'TCGA_G
Cuts at: 0 2897 5172
Size: 2897 2275
XmaI C'CCGG_G
Cuts at: 0 1470 5172
Size: 1470 3702
XmnI GAAnn'nnTTC
Cuts at: 0 108 1172 2291 4592 5172
Size: 108 1064 1119 2301 580
Fragments arranged by size:
2301 1119 1064 580 108
ZraI GAC'GTC
Cuts at: 0 1404 5172
Size: 1404 3768
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AhdI AlfI AloI
AlwNI ApaLI ApoI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BciVI BclI BfrBI BglI BglII Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtsI CspCI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FseI HaeII Hin4I
HincII HindIII MfeI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NsiI NspI PasI PflMI PfoI PmlI
PpuMI PsiI PspXI PstI PvuII SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI SmaI SmlI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AgeI AleI ApaI AscI
AseI AsiSI AvrII BbeI BcgI BlpI BmtI BsmBI
BspMI BsrBI BssHII BstEII BstZ17I BtgZI ClaI DraI
DraIII DrdI FspI FspAI HpaI KasI KpnI MluI
NarI NdeI NheI NotI NruI PacI PciI PmeI
PpiI PshAI PspOMI PsrI PvuI RsrII SanDI SapI
SfoI SgfI SgrAI SnaBI SpeI SrfI SwaI