Restriction map for
pF1KSDA0171
(Linear) (Six-base) MAPSORT of: KIAA0171.seq Check: 3913 from: 1 to: 1875
KIAA0171.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 917 929 1244 1875
Size: 917 12 315 631
Fragments arranged by size:
917 631 315 12
AflIII A'CryG_T
Cuts at: 0 8 1875
Size: 8 1867
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1432 1875
Size: 1432 443
AlwNI CAG_nnn'CTG
Cuts at: 0 1060 1153 1875
Size: 1060 93 722
Fragments arranged by size:
1060 722 93
ApoI r'AATT_y
Cuts at: 0 409 745 1254 1320 1854 1875
Size: 409 336 509 66 534 21
Fragments arranged by size:
534 509 409 336 66 21
BanI G'GyrC_C
Cuts at: 0 994 1675 1875
Size: 994 681 200
Fragments arranged by size:
994 681 200
BanII G_rGCy'C
Cuts at: 0 539 1285 1875
Size: 539 746 590
Fragments arranged by size:
746 590 539
BbsI GAAGACnn'nnnn_
Cuts at: 0 680 923 1875
Size: 680 243 952
Fragments arranged by size:
952 680 243
BglII A'GATC_T
Cuts at: 0 730 1875
Size: 730 1145
BlpI GC'TnA_GC
Cuts at: 0 1053 1875
Size: 1053 822
BmrI ACTGGGnnnn_n'
Cuts at: 0 1433 1875
Size: 1433 442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 1143 1875
Size: 665 478 732
Fragments arranged by size:
732 665 478
Bpu10I CC'TnA_GC
Cuts at: 0 533 1875
Size: 533 1342
BsaI GGTCTCn'nnnn_
Cuts at: 0 799 1875
Size: 799 1076
BsaWI w'CCGG_w
Cuts at: 0 656 1875
Size: 656 1219
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 571 775 1875
Size: 571 204 1100
Fragments arranged by size:
1100 571 204
BseYI C'CCAG_C
Cuts at: 0 1493 1762 1875
Size: 1493 269 113
Fragments arranged by size:
1493 269 113
BsiHKAI G_wGCw'C
Cuts at: 0 1339 1875
Size: 1339 536
BsmI GAATG_Cn'
Cuts at: 0 912 1875
Size: 912 963
Bsp1286I G_dGCh'C
Cuts at: 0 539 1285 1339 1875
Size: 539 746 54 536
Fragments arranged by size:
746 539 536 54
BspEI T'CCGG_A
Cuts at: 0 656 1875
Size: 656 1219
BstEII G'GTnAC_C
Cuts at: 0 970 1875
Size: 970 905
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1686 1875
Size: 1686 189
BstYI r'GATC_y
Cuts at: 0 730 1875
Size: 730 1145
BtgI C'CryG_G
Cuts at: 0 1679 1875
Size: 1679 196
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 716 1875
Size: 716 1159
BtsI GCAGTG_nn'
Cuts at: 0 1188 1875
Size: 1188 687
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 30 65 1875
Size: 30 35 1810
Fragments arranged by size:
1810 35 30
EarI CTCTTCn'nnn_
Cuts at: 0 225 434 717 752 1875
Size: 225 209 283 35 1123
Fragments arranged by size:
1123 283 225 209 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 917 929 1143 1244 1875
Size: 665 252 12 214 101 631
Fragments arranged by size:
665 631 252 214 101 12
EcoO109I rG'GnC_Cy
Cuts at: 0 112 1150 1639 1875
Size: 112 1038 489 236
Fragments arranged by size:
1038 489 236 112
EcoRI G'AATT_C
Cuts at: 0 745 1875
Size: 745 1130
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 310 342 526 558 1036 1068
Size: 58 32 220 32 184 32 478 32
Cuts at: 1068 1875
Size: 807
Fragments arranged by size:
807 478 220 184 58 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1007 1875
Size: 1007 868
MfeI C'AATT_G
Cuts at: 0 632 1875
Size: 632 1243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 1875
Size: 480 1395
MslI CAynn'nnrTG
Cuts at: 0 314 1648 1684 1738 1750 1810 1875
Size: 314 1334 36 54 12 60 65
Fragments arranged by size:
1334 314 65 60 54 36 12
MspA1I CmG'CkG
Cuts at: 0 1021 1057 1198 1759 1875
Size: 1021 36 141 561 116
Fragments arranged by size:
1021 561 141 116 36
NcoI C'CATG_G
Cuts at: 0 1679 1875
Size: 1679 196
NspI r_CATG'y
Cuts at: 0 12 189 1602 1659 1800 1875
Size: 12 177 1413 57 141 75
Fragments arranged by size:
1413 177 141 75 57 12
PciI A'CATG_T
Cuts at: 0 8 1875
Size: 8 1867
PpuMI rG'GwC_Cy
Cuts at: 0 112 1639 1875
Size: 112 1527 236
Fragments arranged by size:
1527 236 112
PsiI TTA'TAA
Cuts at: 0 239 1875
Size: 239 1636
PvuII CAG'CTG
Cuts at: 0 1021 1057 1875
Size: 1021 36 818
Fragments arranged by size:
1021 818 36
SanDI GG'GwC_CC
Cuts at: 0 1639 1875
Size: 1639 236
SapI GCTCTTCn'nnn_
Cuts at: 0 434 1875
Size: 434 1441
SexAI A'CCwGG_T
Cuts at: 0 1470 1875
Size: 1470 405
SphI G_CATG'C
Cuts at: 0 1659 1800 1875
Size: 1659 141 75
Fragments arranged by size:
1659 141 75
SspI AAT'ATT
Cuts at: 0 381 1875
Size: 381 1494
StyI C'CwwG_G
Cuts at: 0 106 143 1679 1875
Size: 106 37 1536 196
Fragments arranged by size:
1536 196 106 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 544 576 822 854 1875
Size: 544 32 246 32 1021
Fragments arranged by size:
1021 544 246 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 167 1875
Size: 167 1708
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApoI BanI BanII BbsI
BglII BlpI BmrI BpmI Bpu10I BsaI BsaWI BseRI
BseYI BsiHKAI BsmI Bsp1286I BspEI BstEII BstXI BstYI
BtgI BtgZI BtsI CspCI EarI Eco57MI EcoO109I EcoRI
Hin4I HincII MfeI MmeI MslI MspA1I NcoI NspI
PciI PpuMI PsiI PvuII SanDI SapI SexAI SphI
SspI StyI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI Bme1580I BmgBI BmtI BplI BpuEI
BsaAI BsaBI BsaHI BsaXI BsgI BsiEI BsiWI BsmBI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstZ17I Bsu36I ClaI DraI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MluI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI StuI SwaI
TaqII TatI Tth111I XbaI XcmI XhoI XmaI ZraI