Restriction map for
pF1KSDA0157
(Linear) (Six-base) MAPSORT of: KIAA0157.seq Check: 7226 from: 1 to: 1245
KIAA0157.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AclI AA'CG_TT
Cuts at: 0 109 1245
Size: 109 1136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 372 426 512 631 890 1066 1245
Size: 12 360 54 86 119 259 176 179
Fragments arranged by size:
360 259 179 176 119 86 54 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 823 1245
Size: 823 422
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 817 849 1245
Size: 817 32 396
Fragments arranged by size:
817 396 32
ApoI r'AATT_y
Cuts at: 0 145 1134 1245
Size: 145 989 111
Fragments arranged by size:
989 145 111
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1033 1066 1245
Size: 1033 33 179
Fragments arranged by size:
1033 179 33
BamHI G'GATC_C
Cuts at: 0 906 1245
Size: 906 339
BanII G_rGCy'C
Cuts at: 0 1015 1245
Size: 1015 230
BbsI GAAGACnn'nnnn_
Cuts at: 0 518 741 1245
Size: 518 223 504
Fragments arranged by size:
518 504 223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 884 918 1245
Size: 884 34 327
Fragments arranged by size:
884 327 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1061 1245
Size: 1061 184
Bme1580I G_kGCm'C
Cuts at: 0 368 1245
Size: 368 877
BmrI ACTGGGnnnn_n'
Cuts at: 0 94 1245
Size: 94 1151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 983 1245
Size: 983 262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1115 1245
Size: 1115 130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 986 1245
Size: 986 259
BsaHI Gr'CG_yC
Cuts at: 0 7 1245
Size: 7 1238
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 244 902 1067 1245
Size: 244 658 165 178
Fragments arranged by size:
658 244 178 165
BseYI C'CCAG_C
Cuts at: 0 828 1245
Size: 828 417
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 1245
Size: 692 553
BsiHKAI G_wGCw'C
Cuts at: 0 440 1245
Size: 440 805
Bsp1286I G_dGCh'C
Cuts at: 0 368 440 1015 1245
Size: 368 72 575 230
Fragments arranged by size:
575 368 230 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 318 1245
Size: 318 927
BstAPI GCAn_nnn'nTGC
Cuts at: 0 670 1245
Size: 670 575
BstYI r'GATC_y
Cuts at: 0 906 1245
Size: 906 339
BtgI C'CryG_G
Cuts at: 0 1196 1245
Size: 1196 49
BtsI GCAGTG_nn'
Cuts at: 0 401 671 797 1245
Size: 401 270 126 448
Fragments arranged by size:
448 401 270 126
EarI CTCTTCn'nnn_
Cuts at: 0 391 445 1020 1245
Size: 391 54 575 225
Fragments arranged by size:
575 391 225 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 372 426 512 631 890 1066 1115
Size: 12 360 54 86 119 259 176 49
Cuts at: 1115 1245
Size: 130
Fragments arranged by size:
360 259 176 130 119 86 54 49
12
EcoO109I rG'GnC_Cy
Cuts at: 0 1215 1245
Size: 1215 30
EcoRV GAT'ATC
Cuts at: 0 473 1245
Size: 473 772
HaeII r_GCGC'y
Cuts at: 0 53 1245
Size: 53 1192
HindIII A'AGCT_T
Cuts at: 0 819 1245
Size: 819 426
MspA1I CmG'CkG
Cuts at: 0 868 1093 1245
Size: 868 225 152
Fragments arranged by size:
868 225 152
NcoI C'CATG_G
Cuts at: 0 1196 1245
Size: 1196 49
PfoI T'CCnGG_A
Cuts at: 0 1053 1245
Size: 1053 192
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 314 346 1245
Size: 314 32 899
Fragments arranged by size:
899 314 32
PpuMI rG'GwC_Cy
Cuts at: 0 1215 1245
Size: 1215 30
PsiI TTA'TAA
Cuts at: 0 638 1245
Size: 638 607
PvuII CAG'CTG
Cuts at: 0 868 1093 1245
Size: 868 225 152
Fragments arranged by size:
868 225 152
SapI GCTCTTCn'nnn_
Cuts at: 0 445 1020 1245
Size: 445 575 225
Fragments arranged by size:
575 445 225
ScaI AGT'ACT
Cuts at: 0 954 1245
Size: 954 291
SfcI C'TryA_G
Cuts at: 0 318 1148 1245
Size: 318 830 97
Fragments arranged by size:
830 318 97
SmlI C'TyrA_G
Cuts at: 0 1001 1245
Size: 1001 244
StuI AGG'CCT
Cuts at: 0 901 1245
Size: 901 344
StyI C'CwwG_G
Cuts at: 0 1196 1245
Size: 1196 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 266 950 1245
Size: 266 684 295
Fragments arranged by size:
684 295 266
TatI w'GTAC_w
Cuts at: 0 515 880 952 1245
Size: 515 365 72 293
Fragments arranged by size:
515 365 293 72
Tth111I GACn'n_nGTC
Cuts at: 0 373 1245
Size: 373 872
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1090 1245
Size: 1090 155
Enzymes that do cut:
AclI AcuI AlfI AloI ApoI BaeI BamHI BanII
BbsI BcgI BciVI Bme1580I BmrI BplI BpmI BpuEI
BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BstAPI
BstYI BtgI BtsI EarI Eco57MI EcoO109I EcoRV HaeII
HindIII MspA1I NcoI PfoI PpiI PpuMI PsiI PvuII
SapI ScaI SfcI SmlI StuI StyI TaqII TatI
Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BanI BbeI BbvCI BclI BfrBI BglI
BglII BlpI BmgBI BmtI Bpu10I BsaI BsaAI BsaBI
BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI FalI
FseI FspI FspAI Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PshAI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TstI XbaI XhoI XmaI
XmnI ZraI