Restriction map for
pF1KA0156
(Linear) (Six-base) MAPSORT of: KIAA0156.seq Check: 1525 from: 1 to: 2889
KIAA0156.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
Acc65I G'GTAC_C
Cuts at: 0 1151 2889
Size: 1151 1738
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 455 1387 2083 2199 2263 2889
Size: 11 444 932 696 116 64 626
Fragments arranged by size:
932 696 626 444 116 64 11
AflIII A'CryG_T
Cuts at: 0 503 1526 2889
Size: 503 1023 1363
Fragments arranged by size:
1363 1023 503
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1480 2889
Size: 1480 1409
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1653 1685 2889
Size: 1653 32 1204
Fragments arranged by size:
1653 1204 32
AlwNI CAG_nnn'CTG
Cuts at: 0 873 2286 2889
Size: 873 1413 603
Fragments arranged by size:
1413 873 603
ApaI G_GGCC'C
Cuts at: 0 59 1997 2889
Size: 59 1938 892
Fragments arranged by size:
1938 892 59
ApaLI G'TGCA_C
Cuts at: 0 1608 2889
Size: 1608 1281
AvaI C'yCGr_G
Cuts at: 0 34 238 1475 1811 2889
Size: 34 204 1237 336 1078
Fragments arranged by size:
1237 1078 336 204 34
BamHI G'GATC_C
Cuts at: 0 1040 2889
Size: 1040 1849
BanI G'GyrC_C
Cuts at: 0 918 1151 2473 2673 2841 2889
Size: 918 233 1322 200 168 48
Fragments arranged by size:
1322 918 233 200 168 48
BanII G_rGCy'C
Cuts at: 0 59 235 443 639 1560 1812 1997 2030
Size: 59 176 208 196 921 252 185 33
Cuts at: 2030 2889
Size: 859
Fragments arranged by size:
921 859 252 208 196 185 176 59
33
BbsI GAAGACnn'nnnn_
Cuts at: 0 827 2889
Size: 827 2062
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1456 1490 1920 1954 2225 2259 2889
Size: 1456 34 430 34 271 34 630
Fragments arranged by size:
1456 630 430 271 34 34 34
BclI T'GATC_A
Cuts at: 0 353 1182 2889
Size: 353 829 1707
Fragments arranged by size:
1707 829 353
BglI GCCn_nnn'nGGC
Cuts at: 0 2281 2839 2889
Size: 2281 558 50
Fragments arranged by size:
2281 558 50
Bme1580I G_kGCm'C
Cuts at: 0 59 1612 1997 2757 2889
Size: 59 1553 385 760 132
Fragments arranged by size:
1553 760 385 132 59
BmgBI CAC'GTC
Cuts at: 0 1632 2889
Size: 1632 1257
BmrI ACTGGGnnnn_n'
Cuts at: 0 1440 2889
Size: 1440 1449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 322 469 895 1296 1345 1982 2308
Size: 307 15 147 426 401 49 637 326
Cuts at: 2308 2889
Size: 581
Fragments arranged by size:
637 581 426 401 326 307 147 49
15
Bpu10I CC'TnA_GC
Cuts at: 0 364 2164 2889
Size: 364 1800 725
Fragments arranged by size:
1800 725 364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 751 2889
Size: 628 123 2138
Fragments arranged by size:
2138 628 123
BsaI GGTCTCn'nnnn_
Cuts at: 0 2656 2889
Size: 2656 233
BsaAI yAC'GTr
Cuts at: 0 504 2250 2535 2889
Size: 504 1746 285 354
Fragments arranged by size:
1746 504 354 285
BsaHI Gr'CG_yC
Cuts at: 0 2560 2889
Size: 2560 329
BsaWI w'CCGG_w
Cuts at: 0 2297 2889
Size: 2297 592
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 235 265 295 298 325 328 2889
Size: 235 30 30 3 27 3 2561
Fragments arranged by size:
2561 235 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 283 286 319 1299 1342 2624 2771
Size: 88 195 3 33 980 43 1282 147
Cuts at: 2771 2889
Size: 118
Fragments arranged by size:
1282 980 195 147 118 88 43 33
3
BseYI C'CCAG_C
Cuts at: 0 52 1571 1999 2826 2889
Size: 52 1519 428 827 63
Fragments arranged by size:
1519 827 428 63 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1576 1792 2071 2889
Size: 1576 216 279 818
Fragments arranged by size:
1576 818 279 216
BsiEI CG_ry'CG
Cuts at: 0 14 2889
Size: 14 2875
BsiHKAI G_wGCw'C
Cuts at: 0 235 443 503 1560 1612 1631 1812 2889
Size: 235 208 60 1057 52 19 181 1077
Fragments arranged by size:
1077 1057 235 208 181 60 52 19
BsmI GAATG_Cn'
Cuts at: 0 983 1227 2889
Size: 983 244 1662
Fragments arranged by size:
1662 983 244
Bsp1286I G_dGCh'C
Cuts at: 0 59 235 443 503 639 1560 1612 1631
Size: 59 176 208 60 136 921 52 19
Cuts at: 1631 1812 1997 2030 2757 2889
Size: 181 185 33 727 132
Fragments arranged by size:
921 727 208 185 181 176 136 132
60 59 52 33 19
BspHI T'CATG_A
Cuts at: 0 1496 2889
Size: 1496 1393
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2138 2889
Size: 2138 751
BsrFI r'CCGG_y
Cuts at: 0 1225 2889
Size: 1225 1664
BsrGI T'GTAC_A
Cuts at: 0 1098 2889
Size: 1098 1791
BssHII G'CGCG_C
Cuts at: 0 1002 2889
Size: 1002 1887
BssSI C'ACGA_G
Cuts at: 0 1349 1721 2750 2889
Size: 1349 372 1029 139
Fragments arranged by size:
1349 1029 372 139
BstBI TT'CG_AA
Cuts at: 0 2035 2889
Size: 2035 854
BstEII G'GTnAC_C
Cuts at: 0 2496 2889
Size: 2496 393
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1037 2889
Size: 1037 1852
BstYI r'GATC_y
Cuts at: 0 1040 2201 2889
Size: 1040 1161 688
Fragments arranged by size:
1161 1040 688
Bsu36I CC'TnA_GG
Cuts at: 0 1282 2488 2783 2889
Size: 1282 1206 295 106
Fragments arranged by size:
1282 1206 295 106
BtgI C'CryG_G
Cuts at: 0 149 212 224 1163 1652 2223 2804 2889
Size: 149 63 12 939 489 571 581 85
Fragments arranged by size:
939 581 571 489 149 85 63 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 209 1916 2889
Size: 209 1707 973
Fragments arranged by size:
1707 973 209
BtsI GCAGTG_nn'
Cuts at: 0 1575 2889
Size: 1575 1314
ClaI AT'CG_AT
Cuts at: 0 950 2889
Size: 950 1939
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1380 1415 2889
Size: 1380 35 1474
Fragments arranged by size:
1474 1380 35
DraI TTT'AAA
Cuts at: 0 2259 2889
Size: 2259 630
DraIII CAC_nnn'GTG
Cuts at: 0 2680 2889
Size: 2680 209
DrdI GACnn_nn'nnGTC
Cuts at: 0 2736 2889
Size: 2736 153
EaeI y'GGCC_r
Cuts at: 0 11 128 567 1160 2889
Size: 11 117 439 593 1729
Fragments arranged by size:
1729 593 439 117 11
EagI C'GGCC_G
Cuts at: 0 11 2889
Size: 11 2878
EarI CTCTTCn'nnn_
Cuts at: 0 260 431 1376 1907 2059 2179 2258 2889
Size: 260 171 945 531 152 120 79 631
Fragments arranged by size:
945 631 531 260 171 152 120 79
EcoICRI GAG'CTC
Cuts at: 0 233 441 1558 1810 2889
Size: 233 208 1117 252 1079
Fragments arranged by size:
1117 1079 252 233 208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 307 322 455 469 895 1296 1345
Size: 11 296 15 133 14 426 401 49
Cuts at: 1345 1387 1982 2083 2199 2263 2308 2889
Size: 42 595 101 116 64 45 581
Fragments arranged by size:
595 581 426 401 296 133 116 101
64 49 45 42 15 14 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 682 2889
Size: 682 2207
EcoO109I rG'GnC_Cy
Cuts at: 0 1993 2484 2523 2889
Size: 1993 491 39 366
Fragments arranged by size:
1993 491 366 39
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 397 622 1783 2889
Size: 397 225 1161 1106
Fragments arranged by size:
1161 1106 397 225
HaeII r_GCGC'y
Cuts at: 0 2766 2889
Size: 2766 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 298 330 662 694 1389 1421
Size: 28 32 238 32 332 32 695 32
Cuts at: 1421 1431 1463 2889
Size: 10 32 1426
Fragments arranged by size:
1426 695 332 238 32 32 32 32
32 28 10
HindIII A'AGCT_T
Cuts at: 0 1214 2889
Size: 1214 1675
KpnI G_GTAC'C
Cuts at: 0 1155 2889
Size: 1155 1734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2229 2889
Size: 2229 660
MscI TGG'CCA
Cuts at: 0 569 1162 2889
Size: 569 593 1727
Fragments arranged by size:
1727 593 569
MslI CAynn'nnrTG
Cuts at: 0 506 2614 2889
Size: 506 2108 275
Fragments arranged by size:
2108 506 275
MspA1I CmG'CkG
Cuts at: 0 133 226 285 873 1960 1999 2736 2806
Size: 133 93 59 588 1087 39 737 70
Cuts at: 2806 2889
Size: 83
Fragments arranged by size:
1087 737 588 133 93 83 70 59
39
NaeI GCC'GGC
Cuts at: 0 1227 2889
Size: 1227 1662
NcoI C'CATG_G
Cuts at: 0 149 212 1163 1652 2889
Size: 149 63 951 489 1237
Fragments arranged by size:
1237 951 489 149 63
NdeI CA'TA_TG
Cuts at: 0 659 2889
Size: 659 2230
NgoMIV G'CCGG_C
Cuts at: 0 1225 2889
Size: 1225 1664
NspI r_CATG'y
Cuts at: 0 1467 1530 2076 2145 2889
Size: 1467 63 546 69 744
Fragments arranged by size:
1467 744 546 69 63
PciI A'CATG_T
Cuts at: 0 1526 2889
Size: 1526 1363
PflMI CCAn_nnn'nTGG
Cuts at: 0 1529 2889
Size: 1529 1360
PmlI CAC'GTG
Cuts at: 0 504 2889
Size: 504 2385
PpuMI rG'GwC_Cy
Cuts at: 0 2484 2889
Size: 2484 405
PspOMI G'GGCC_C
Cuts at: 0 55 1993 2889
Size: 55 1938 896
Fragments arranged by size:
1938 896 55
PstI C_TGCA'G
Cuts at: 0 1752 1959 2762 2778 2817 2889
Size: 1752 207 803 16 39 72
Fragments arranged by size:
1752 803 207 72 39 16
PvuII CAG'CTG
Cuts at: 0 285 873 1960 2736 2889
Size: 285 588 1087 776 153
Fragments arranged by size:
1087 776 588 285 153
SacI G_AGCT'C
Cuts at: 0 235 443 1560 1812 2889
Size: 235 208 1117 252 1077
Fragments arranged by size:
1117 1077 252 235 208
SacII CC_GC'GG
Cuts at: 0 227 2807 2889
Size: 227 2580 82
Fragments arranged by size:
2580 227 82
SapI GCTCTTCn'nnn_
Cuts at: 0 431 1376 1907 2889
Size: 431 945 531 982
Fragments arranged by size:
982 945 531 431
ScaI AGT'ACT
Cuts at: 0 574 2889
Size: 574 2315
SexAI A'CCwGG_T
Cuts at: 0 2676 2889
Size: 2676 213
SfcI C'TryA_G
Cuts at: 0 865 1748 1955 2136 2758 2774 2813 2889
Size: 865 883 207 181 622 16 39 76
Fragments arranged by size:
883 865 622 207 181 76 39 16
SmaI CCC'GGG
Cuts at: 0 240 2889
Size: 240 2649
SmlI C'TyrA_G
Cuts at: 0 607 730 2889
Size: 607 123 2159
Fragments arranged by size:
2159 607 123
SphI G_CATG'C
Cuts at: 0 2145 2889
Size: 2145 744
StuI AGG'CCT
Cuts at: 0 2182 2420 2889
Size: 2182 238 469
Fragments arranged by size:
2182 469 238
StyI C'CwwG_G
Cuts at: 0 47 59 149 212 386 922 1163 1355
Size: 47 12 90 63 174 536 241 192
Cuts at: 1355 1652 2078 2889
Size: 297 426 811
Fragments arranged by size:
811 536 426 297 241 192 174 90
63 47 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1585 2859 2889
Size: 1585 1274 30
Fragments arranged by size:
1585 1274 30
TatI w'GTAC_w
Cuts at: 0 572 1098 2434 2889
Size: 572 526 1336 455
Fragments arranged by size:
1336 572 526 455
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 235 267 2889
Size: 235 32 2622
Fragments arranged by size:
2622 235 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 393 577 1248 1320 2889
Size: 393 184 671 72 1569
Fragments arranged by size:
1569 671 393 184 72
XmaI C'CCGG_G
Cuts at: 0 238 2889
Size: 238 2651
XmnI GAAnn'nnTTC
Cuts at: 0 422 2889
Size: 422 2467
Enzymes that do cut:
Acc65I AcuI AflIII AlfI AloI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbsI BcgI BclI BglI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII
KpnI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NspI PciI PflMI PmlI PpuMI PspOMI PstI
PvuII SacI SacII SapI ScaI SexAI SfcI SmaI
SmlI SphI StuI StyI TaqII TatI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BciVI BfrBI BglII BlpI BmtI BplI BsaBI
BsiWI BsmBI BspEI BsrBI BsrDI BstAPI BstZ17I EciI
EcoRI EcoRV FseI FspI FspAI HincII HpaI KasI
MfeI MluI NarI NheI NotI NruI NsiI PacI
PasI PfoI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SanDI SbfI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
XhoI ZraI