Restriction map for
pF1KSDA0155
(Linear) (Six-base) MAPSORT of: KIAA0155.seq Check: 8726 from: 1 to: 3519
KIAA0155.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AatII G_ACGT'C
Cuts at: 0 2955 3519
Size: 2955 564
AccI GT'mk_AC
Cuts at: 0 349 3519
Size: 349 3170
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1306 1634 1657 2171 2242 2347 2361 2705
Size: 1306 328 23 514 71 105 14 344
Cuts at: 2705 3519
Size: 814
Fragments arranged by size:
1306 814 514 344 328 105 71 23
14
AflIII A'CryG_T
Cuts at: 0 1826 2232 3519
Size: 1826 406 1287
Fragments arranged by size:
1826 1287 406
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 939 1101 3519
Size: 939 162 2418
Fragments arranged by size:
2418 939 162
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 701 733 3519
Size: 701 32 2786
Fragments arranged by size:
2786 701 32
AlwNI CAG_nnn'CTG
Cuts at: 0 870 3519
Size: 870 2649
ApaI G_GGCC'C
Cuts at: 0 2178 2637 3519
Size: 2178 459 882
Fragments arranged by size:
2178 882 459
ApoI r'AATT_y
Cuts at: 0 1136 1534 2755 3519
Size: 1136 398 1221 764
Fragments arranged by size:
1221 1136 764 398
AseI AT'TA_AT
Cuts at: 0 1679 3519
Size: 1679 1840
AvaI C'yCGr_G
Cuts at: 0 1856 2171 2486 3519
Size: 1856 315 315 1033
Fragments arranged by size:
1856 1033 315 315
AvrII C'CTAG_G
Cuts at: 0 1398 1608 3519
Size: 1398 210 1911
Fragments arranged by size:
1911 1398 210
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2364 2397 3519
Size: 2364 33 1122
Fragments arranged by size:
2364 1122 33
BamHI G'GATC_C
Cuts at: 0 3463 3519
Size: 3463 56
BanI G'GyrC_C
Cuts at: 0 1285 1372 2238 3519
Size: 1285 87 866 1281
Fragments arranged by size:
1285 1281 866 87
BanII G_rGCy'C
Cuts at: 0 13 546 672 2178 2637 3519
Size: 13 533 126 1506 459 882
Fragments arranged by size:
1506 882 533 459 126 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 2656 2889 3237 3519
Size: 2656 233 348 282
Fragments arranged by size:
2656 348 282 233
BciVI GTATCCnnnnn_n'
Cuts at: 0 252 1232 3519
Size: 252 980 2287
Fragments arranged by size:
2287 980 252
BclI T'GATC_A
Cuts at: 0 378 3519
Size: 378 3141
BfrBI ATG'CAT
Cuts at: 0 883 1078 3519
Size: 883 195 2441
Fragments arranged by size:
2441 883 195
Bme1580I G_kGCm'C
Cuts at: 0 1290 1377 2178 2493 2637 3519
Size: 1290 87 801 315 144 882
Fragments arranged by size:
1290 882 801 315 144 87
BmgBI CAC'GTC
Cuts at: 0 1496 3519
Size: 1496 2023
BmrI ACTGGGnnnn_n'
Cuts at: 0 2237 2466 2631 3519
Size: 2237 229 165 888
Fragments arranged by size:
2237 888 229 165
BmtI G_CTAG'C
Cuts at: 0 937 1816 3519
Size: 937 879 1703
Fragments arranged by size:
1703 937 879
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3375 3519
Size: 3375 144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1334 2377 3519
Size: 1334 1043 1142
Fragments arranged by size:
1334 1142 1043
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 3519
Size: 439 3080
BsaI GGTCTCn'nnnn_
Cuts at: 0 221 3519
Size: 221 3298
BsaAI yAC'GTr
Cuts at: 0 202 3519
Size: 202 3317
BsaHI Gr'CG_yC
Cuts at: 0 1426 2952 3519
Size: 1426 1526 567
Fragments arranged by size:
1526 1426 567
BsaWI w'CCGG_w
Cuts at: 0 72 3289 3519
Size: 72 3217 230
Fragments arranged by size:
3217 230 72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 71 101 689 719 3519
Size: 71 30 588 30 2800
Fragments arranged by size:
2800 588 71 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 554 2756 2863 2892 3519
Size: 554 2202 107 29 627
Fragments arranged by size:
2202 627 554 107 29
BseYI C'CCAG_C
Cuts at: 0 3366 3519
Size: 3366 153
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 1354 3340 3519
Size: 106 1248 1986 179
Fragments arranged by size:
1986 1248 179 106
BsiHKAI G_wGCw'C
Cuts at: 0 546 1025 1896 3519
Size: 546 479 871 1623
Fragments arranged by size:
1623 871 546 479
BsmI GAATG_Cn'
Cuts at: 0 658 883 2209 3519
Size: 658 225 1326 1310
Fragments arranged by size:
1326 1310 658 225
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2596 3519
Size: 2596 923
Bsp1286I G_dGCh'C
Cuts at: 0 13 546 672 1025 1290 1377 1896 2178
Size: 13 533 126 353 265 87 519 282
Cuts at: 2178 2493 2637 3519
Size: 315 144 882
Fragments arranged by size:
882 533 519 353 315 282 265 144
126 87 13
BsrBI CCG'CTC
Cuts at: 0 2514 3519
Size: 2514 1005
BsrDI GCAATG_nn'
Cuts at: 0 565 615 3519
Size: 565 50 2904
Fragments arranged by size:
2904 565 50
BsrFI r'CCGG_y
Cuts at: 0 3208 3519
Size: 3208 311
BsrGI T'GTAC_A
Cuts at: 0 279 3519
Size: 279 3240
BssSI C'ACGA_G
Cuts at: 0 1997 3519
Size: 1997 1522
BstXI CCAn_nnnn'nTGG
Cuts at: 0 693 1700 3439 3519
Size: 693 1007 1739 80
Fragments arranged by size:
1739 1007 693 80
BstYI r'GATC_y
Cuts at: 0 321 2905 3409 3463 3519
Size: 321 2584 504 54 56
Fragments arranged by size:
2584 504 321 56 54
BstZ17I GTA'TAC
Cuts at: 0 350 3519
Size: 350 3169
BtgI C'CryG_G
Cuts at: 0 1616 3519
Size: 1616 1903
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1540 3519
Size: 1540 1979
BtsI GCAGTG_nn'
Cuts at: 0 2452 2832 3519
Size: 2452 380 687
Fragments arranged by size:
2452 687 380
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 984 1019 3519
Size: 984 35 2500
Fragments arranged by size:
2500 984 35
DraIII CAC_nnn'GTG
Cuts at: 0 1795 2245 3519
Size: 1795 450 1274
Fragments arranged by size:
1795 1274 450
EaeI y'GGCC_r
Cuts at: 0 356 1898 3429 3519
Size: 356 1542 1531 90
Fragments arranged by size:
1542 1531 356 90
EarI CTCTTCn'nnn_
Cuts at: 0 167 393 1676 2185 2504 2576 2600 2800
Size: 167 226 1283 509 319 72 24 200
Cuts at: 2800 3063 3519
Size: 263 456
Fragments arranged by size:
1283 509 456 319 263 226 200 167
72 24
EcoICRI GAG'CTC
Cuts at: 0 544 3519
Size: 544 2975
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1306 1334 1634 1657 2171 2242 2347 2361
Size: 1306 28 300 23 514 71 105 14
Cuts at: 2361 2377 2705 3519
Size: 16 328 814
Fragments arranged by size:
1306 814 514 328 300 105 71 28
23 16 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 418 1402 3519
Size: 418 984 2117
Fragments arranged by size:
2117 984 418
EcoO109I rG'GnC_Cy
Cuts at: 0 1744 2175 3284 3519
Size: 1744 431 1109 235
Fragments arranged by size:
1744 1109 431 235
EcoRI G'AATT_C
Cuts at: 0 1534 3519
Size: 1534 1985
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1653 3519
Size: 1653 1866
HaeII r_GCGC'y
Cuts at: 0 145 2099 3519
Size: 145 1954 1420
Fragments arranged by size:
1954 1420 145
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 912 944 1687 1701 1719 1733 2871 2903
Size: 912 32 743 14 18 14 1138 32
Cuts at: 2903 3235 3253 3267 3285 3298 3330 3382 3394
Size: 332 18 14 18 13 32 52 12
Cuts at: 3394 3414 3426 3475 3507 3519
Size: 20 12 49 32 12
Fragments arranged by size:
1138 912 743 332 52 49 32 32
32 32 20 18 18 18 14 14
14 13 12 12 12
HincII GTy'rAC
Cuts at: 0 1593 3519
Size: 1593 1926
HindIII A'AGCT_T
Cuts at: 0 506 971 1106 1238 3519
Size: 506 465 135 132 2281
Fragments arranged by size:
2281 506 465 135 132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 687 3491 3519
Size: 170 517 2804 28
Fragments arranged by size:
2804 517 170 28
MscI TGG'CCA
Cuts at: 0 1900 3431 3519
Size: 1900 1531 88
Fragments arranged by size:
1900 1531 88
MslI CAynn'nnrTG
Cuts at: 0 1083 1694 2069 2071 3519
Size: 1083 611 375 2 1448
Fragments arranged by size:
1448 1083 611 375 2
MspA1I CmG'CkG
Cuts at: 0 592 2631 3237 3519
Size: 592 2039 606 282
Fragments arranged by size:
2039 606 592 282
NaeI GCC'GGC
Cuts at: 0 3210 3519
Size: 3210 309
NcoI C'CATG_G
Cuts at: 0 1616 3519
Size: 1616 1903
NdeI CA'TA_TG
Cuts at: 0 127 3519
Size: 127 3392
NgoMIV G'CCGG_C
Cuts at: 0 3208 3519
Size: 3208 311
NheI G'CTAG_C
Cuts at: 0 933 1812 3519
Size: 933 879 1707
Fragments arranged by size:
1707 933 879
NsiI A_TGCA'T
Cuts at: 0 885 1080 3519
Size: 885 195 2439
Fragments arranged by size:
2439 885 195
NspI r_CATG'y
Cuts at: 0 2236 3519
Size: 2236 1283
PciI A'CATG_T
Cuts at: 0 2232 3519
Size: 2232 1287
PflMI CCAn_nnn'nTGG
Cuts at: 0 393 870 1033 2643 3519
Size: 393 477 163 1610 876
Fragments arranged by size:
1610 876 477 393 163
PfoI T'CCnGG_A
Cuts at: 0 29 3519
Size: 29 3490
PpuMI rG'GwC_Cy
Cuts at: 0 1744 3519
Size: 1744 1775
PshAI GACnn'nnGTC
Cuts at: 0 3237 3519
Size: 3237 282
PspOMI G'GGCC_C
Cuts at: 0 2174 2633 3519
Size: 2174 459 886
Fragments arranged by size:
2174 886 459
SacI G_AGCT'C
Cuts at: 0 546 3519
Size: 546 2973
SapI GCTCTTCn'nnn_
Cuts at: 0 393 1676 2504 2600 3519
Size: 393 1283 828 96 919
Fragments arranged by size:
1283 919 828 393 96
ScaI AGT'ACT
Cuts at: 0 982 1916 2336 3519
Size: 982 934 420 1183
Fragments arranged by size:
1183 982 934 420
SfcI C'TryA_G
Cuts at: 0 222 2432 3519
Size: 222 2210 1087
Fragments arranged by size:
2210 1087 222
SmaI CCC'GGG
Cuts at: 0 2173 2488 3519
Size: 2173 315 1031
Fragments arranged by size:
2173 1031 315
SmlI C'TyrA_G
Cuts at: 0 418 3519
Size: 418 3101
SrfI GCCC'GGGC
Cuts at: 0 2173 2488 3519
Size: 2173 315 1031
Fragments arranged by size:
2173 1031 315
SspI AAT'ATT
Cuts at: 0 486 1417 2661 3519
Size: 486 931 1244 858
Fragments arranged by size:
1244 931 858 486
StuI AGG'CCT
Cuts at: 0 3478 3519
Size: 3478 41
StyI C'CwwG_G
Cuts at: 0 1190 1398 1608 1616 1818 2273 3432 3519
Size: 1190 208 210 8 202 455 1159 87
Fragments arranged by size:
1190 1159 455 210 208 202 87 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1360 2256 3519
Size: 1360 896 1263
Fragments arranged by size:
1360 1263 896
TatI w'GTAC_w
Cuts at: 0 279 462 980 1914 2087 2157 2334 3519
Size: 279 183 518 934 173 70 177 1185
Fragments arranged by size:
1185 934 518 279 183 177 173 70
Tth111I GACn'n_nGTC
Cuts at: 0 43 3519
Size: 43 3476
XbaI T'CTAG_A
Cuts at: 0 720 3519
Size: 720 2799
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1387 3193 3519
Size: 1387 1806 326
Fragments arranged by size:
1806 1387 326
XmaI C'CCGG_G
Cuts at: 0 2171 2486 3519
Size: 2171 315 1033
Fragments arranged by size:
2171 1033 315
XmnI GAAnn'nnTTC
Cuts at: 0 1670 3519
Size: 1670 1849
ZraI GAC'GTC
Cuts at: 0 2953 3519
Size: 2953 566
Enzymes that do cut:
AatII AccI AcuI AflIII AlfI AloI AlwNI ApaI
ApoI AseI AvaI AvrII BaeI BamHI BanI BanII
BbsI BciVI BclI BfrBI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BsrBI
BsrDI BsrFI BsrGI BssSI BstXI BstYI BstZ17I BtgI
BtgZI BtsI CspCI DraIII EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NsiI NspI PciI PflMI PfoI PpuMI PshAI
PspOMI SacI SapI ScaI SfcI SmaI SmlI SrfI
SspI StuI StyI TaqII TatI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
ApaLI AscI AsiSI BbeI BbvCI BcgI BglI BglII
BlpI Bpu10I BsaBI BsiEI BsiWI BspEI BspHI BspMI
BssHII BstAPI BstBI BstEII Bsu36I ClaI DraI DrdI
EagI EciI EcoRV FseI FspI FspAI HpaI KasI
KpnI MfeI MluI NarI NotI NruI PacI PasI
PmeI PmlI PpiI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SwaI TstI
XhoI