Restriction map for
pF1KSDA0152
(Linear) (Six-base) MAPSORT of: KIAA0152.seq Check: 4462 from: 1 to: 876
KIAA0152.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AccI GT'mk_AC
Cuts at: 0 389 876
Size: 389 487
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 876
Size: 646 230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 271 876
Size: 271 605
AlwNI CAG_nnn'CTG
Cuts at: 0 291 876
Size: 291 585
ApaLI G'TGCA_C
Cuts at: 0 184 617 876
Size: 184 433 259
Fragments arranged by size:
433 259 184
ApoI r'AATT_y
Cuts at: 0 376 876
Size: 376 500
AvaI C'yCGr_G
Cuts at: 0 85 133 876
Size: 85 48 743
Fragments arranged by size:
743 85 48
BamHI G'GATC_C
Cuts at: 0 191 876
Size: 191 685
BanII G_rGCy'C
Cuts at: 0 92 876
Size: 92 784
BbsI GAAGACnn'nnnn_
Cuts at: 0 834 876
Size: 834 42
BglI GCCn_nnn'nGGC
Cuts at: 0 106 115 227 876
Size: 106 9 112 649
Fragments arranged by size:
649 112 106 9
Bme1580I G_kGCm'C
Cuts at: 0 188 341 621 876
Size: 188 153 280 255
Fragments arranged by size:
280 255 188 153
BmgBI CAC'GTC
Cuts at: 0 183 441 876
Size: 183 258 435
Fragments arranged by size:
435 258 183
BsaI GGTCTCn'nnnn_
Cuts at: 0 313 876
Size: 313 563
BsaAI yAC'GTr
Cuts at: 0 363 876
Size: 363 513
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 331 364 876
Size: 331 33 512
Fragments arranged by size:
512 331 33
BseYI C'CCAG_C
Cuts at: 0 3 404 634 876
Size: 3 401 230 242
Fragments arranged by size:
401 242 230 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 876
Size: 781 95
BsiHKAI G_wGCw'C
Cuts at: 0 188 621 876
Size: 188 433 255
Fragments arranged by size:
433 255 188
Bsp1286I G_dGCh'C
Cuts at: 0 92 188 341 621 876
Size: 92 96 153 280 255
Fragments arranged by size:
280 255 153 96 92
BsrBI CCG'CTC
Cuts at: 0 306 876
Size: 306 570
BsrFI r'CCGG_y
Cuts at: 0 69 100 109 869 876
Size: 69 31 9 760 7
Fragments arranged by size:
760 69 31 9 7
BstXI CCAn_nnnn'nTGG
Cuts at: 0 828 876
Size: 828 48
BstYI r'GATC_y
Cuts at: 0 191 287 876
Size: 191 96 589
Fragments arranged by size:
589 191 96
Bsu36I CC'TnA_GG
Cuts at: 0 278 876
Size: 278 598
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 144 179 876
Size: 144 35 697
Fragments arranged by size:
697 144 35
DraIII CAC_nnn'GTG
Cuts at: 0 444 876
Size: 444 432
EaeI y'GGCC_r
Cuts at: 0 98 107 435 876
Size: 98 9 328 441
Fragments arranged by size:
441 328 98 9
EarI CTCTTCn'nnn_
Cuts at: 0 686 862 876
Size: 686 176 14
Fragments arranged by size:
686 176 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 876
Size: 646 230
EcoNI CCTnn'n_nnAGG
Cuts at: 0 215 876
Size: 215 661
EcoO109I rG'GnC_Cy
Cuts at: 0 82 207 769 876
Size: 82 125 562 107
Fragments arranged by size:
562 125 107 82
EcoRV GAT'ATC
Cuts at: 0 462 876
Size: 462 414
HaeII r_GCGC'y
Cuts at: 0 40 876
Size: 40 836
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 74 106 279 311 709 741 876
Size: 74 32 173 32 398 32 135
Fragments arranged by size:
398 173 135 74 32 32 32
HincII GTy'rAC
Cuts at: 0 156 876
Size: 156 720
HindIII A'AGCT_T
Cuts at: 0 658 876
Size: 658 218
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 874 876
Size: 296 578 2
Fragments arranged by size:
578 296 2
MscI TGG'CCA
Cuts at: 0 437 876
Size: 437 439
MslI CAynn'nnrTG
Cuts at: 0 826 876
Size: 826 50
MspA1I CmG'CkG
Cuts at: 0 31 876
Size: 31 845
NaeI GCC'GGC
Cuts at: 0 71 102 111 876
Size: 71 31 9 765
Fragments arranged by size:
765 71 31 9
NgoMIV G'CCGG_C
Cuts at: 0 69 100 109 876
Size: 69 31 9 767
Fragments arranged by size:
767 69 31 9
NspI r_CATG'y
Cuts at: 0 178 876
Size: 178 698
PflMI CCAn_nnn'nTGG
Cuts at: 0 444 876
Size: 444 432
PfoI T'CCnGG_A
Cuts at: 0 672 876
Size: 672 204
PpuMI rG'GwC_Cy
Cuts at: 0 82 207 876
Size: 82 125 669
Fragments arranged by size:
669 125 82
SanDI GG'GwC_CC
Cuts at: 0 82 876
Size: 82 794
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 106 227 876
Size: 106 121 649
Fragments arranged by size:
649 121 106
SgrAI Cr'CCGG_yG
Cuts at: 0 69 876
Size: 69 807
SmaI CCC'GGG
Cuts at: 0 87 876
Size: 87 789
StyI C'CwwG_G
Cuts at: 0 608 876
Size: 608 268
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 795 876
Size: 795 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 17 49 519 551 876
Size: 17 32 470 32 325
Fragments arranged by size:
470 325 32 32 17
XmaI C'CCGG_G
Cuts at: 0 85 876
Size: 85 791
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApaLI ApoI AvaI BamHI
BanII BbsI BglI Bme1580I BmgBI BsaI BsaAI BseRI
BseYI BsgI BsiHKAI Bsp1286I BsrBI BsrFI BstXI BstYI
Bsu36I CspCI DraIII EaeI EarI Eco57MI EcoNI EcoO109I
EcoRV HaeII Hin4I HincII HindIII MmeI MscI MslI
MspA1I NaeI NgoMIV NspI PflMI PfoI PpuMI SanDI
SfiI SgrAI SmaI StyI TaqII TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgI BtgZI BtsI ClaI DraI
DrdI EagI EciI EcoICRI EcoRI FalI FseI FspI
FspAI HpaI KasI KpnI MfeI MluI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI Tth111I
XbaI XcmI XhoI XmnI ZraI