Restriction map for
pF1KA0151
(Linear) (Six-base) MAPSORT of: KIAA0151.seq Check: 5883 from: 1 to: 2148
KIAA0151.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 144 383 730 805 1758 1868 2148
Size: 144 239 347 75 953 110 280
Fragments arranged by size:
953 347 280 239 144 110 75
AatII G_ACGT'C
Cuts at: 0 1196 2148
Size: 1196 952
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1128 1158 1486 1945 2148
Size: 1128 30 328 459 203
Fragments arranged by size:
1128 459 328 203 30
AflIII A'CryG_T
Cuts at: 0 548 2148
Size: 548 1600
AleI CACnn'nnGTG
Cuts at: 0 1073 2148
Size: 1073 1075
AlwNI CAG_nnn'CTG
Cuts at: 0 646 1275 1464 2148
Size: 646 629 189 684
Fragments arranged by size:
684 646 629 189
ApaI G_GGCC'C
Cuts at: 0 767 1293 2148
Size: 767 526 855
Fragments arranged by size:
855 767 526
ApaLI G'TGCA_C
Cuts at: 0 1015 1840 2148
Size: 1015 825 308
Fragments arranged by size:
1015 825 308
ApoI r'AATT_y
Cuts at: 0 1737 2148
Size: 1737 411
AvaI C'yCGr_G
Cuts at: 0 482 1081 2148
Size: 482 599 1067
Fragments arranged by size:
1067 599 482
BanI G'GyrC_C
Cuts at: 0 742 831 1258 2148
Size: 742 89 427 890
Fragments arranged by size:
890 742 427 89
BanII G_rGCy'C
Cuts at: 0 301 767 1088 1293 1983 2057 2148
Size: 301 466 321 205 690 74 91
Fragments arranged by size:
690 466 321 301 205 91 74
BbeI G_GCGC'C
Cuts at: 0 1262 2148
Size: 1262 886
BbsI GAAGACnn'nnnn_
Cuts at: 0 108 930 2148
Size: 108 822 1218
Fragments arranged by size:
1218 822 108
BbvCI CC'TCA_GC
Cuts at: 0 1402 2148
Size: 1402 746
BglI GCCn_nnn'nGGC
Cuts at: 0 1871 2148
Size: 1871 277
Bme1580I G_kGCm'C
Cuts at: 0 747 767 836 1019 1293 1844 2148
Size: 747 20 69 183 274 551 304
Fragments arranged by size:
747 551 304 274 183 69 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 820 1341 2148
Size: 820 521 807
Fragments arranged by size:
820 807 521
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 282 1533 2148
Size: 282 1251 615
Fragments arranged by size:
1251 615 282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 313 633 790 874 1333 1503 2148
Size: 252 61 320 157 84 459 170 645
Fragments arranged by size:
645 459 320 252 170 157 84 61
Bpu10I CC'TnA_GC
Cuts at: 0 1402 1558 1982 2148
Size: 1402 156 424 166
Fragments arranged by size:
1402 424 166 156
BsaI GGTCTCn'nnnn_
Cuts at: 0 895 1537 1840 2148
Size: 895 642 303 308
Fragments arranged by size:
895 642 308 303
BsaHI Gr'CG_yC
Cuts at: 0 1193 1259 2148
Size: 1193 66 889
Fragments arranged by size:
1193 889 66
BsaWI w'CCGG_w
Cuts at: 0 91 704 2148
Size: 91 613 1444
Fragments arranged by size:
1444 613 91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 86 116 685 715 1300 1330 2148
Size: 86 30 569 30 585 30 818
Fragments arranged by size:
818 585 569 86 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 235 454 544 1384 1723 1795 2148
Size: 235 219 90 840 339 72 353
Fragments arranged by size:
840 353 339 235 219 90 72
BseYI C'CCAG_C
Cuts at: 0 42 578 727 875 1087 2014 2130 2148
Size: 42 536 149 148 212 927 116 18
Fragments arranged by size:
927 536 212 149 148 116 42 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 946 1312 1810 2148
Size: 172 774 366 498 338
Fragments arranged by size:
774 498 366 338 172
BsiHKAI G_wGCw'C
Cuts at: 0 10 1019 1349 1844 2057 2148
Size: 10 1009 330 495 213 91
Fragments arranged by size:
1009 495 330 213 91 10
BsmI GAATG_Cn'
Cuts at: 0 312 2148
Size: 312 1836
BsmBI CGTCTCn'nnnn_
Cuts at: 0 217 516 1098 2148
Size: 217 299 582 1050
Fragments arranged by size:
1050 582 299 217
Bsp1286I G_dGCh'C
Cuts at: 0 10 301 747 767 836 1019 1088 1293
Size: 10 291 446 20 69 183 69 205
Cuts at: 1293 1349 1844 1983 2057 2148
Size: 56 495 139 74 91
Fragments arranged by size:
495 446 291 205 183 139 91 74
69 69 56 20 10
BspEI T'CCGG_A
Cuts at: 0 91 2148
Size: 91 2057
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 47 143 144 383 545 730 805 1229
Size: 47 96 1 239 162 185 75 424
Cuts at: 1229 1758 1868 2042 2148
Size: 529 110 174 106
Fragments arranged by size:
529 424 239 185 174 162 110 106
96 75 47 1
BsrBI CCG'CTC
Cuts at: 0 558 2148
Size: 558 1590
BsrDI GCAATG_nn'
Cuts at: 0 734 2148
Size: 734 1414
BsrFI r'CCGG_y
Cuts at: 0 236 361 723 1261 2148
Size: 236 125 362 538 887
Fragments arranged by size:
887 538 362 236 125
BsrGI T'GTAC_A
Cuts at: 0 68 2148
Size: 68 2080
BssSI C'ACGA_G
Cuts at: 0 1843 2148
Size: 1843 305
BstBI TT'CG_AA
Cuts at: 0 570 2148
Size: 570 1578
BstEII G'GTnAC_C
Cuts at: 0 1066 2148
Size: 1066 1082
BstXI CCAn_nnnn'nTGG
Cuts at: 0 852 2148
Size: 852 1296
BstYI r'GATC_y
Cuts at: 0 606 1454 2148
Size: 606 848 694
Fragments arranged by size:
848 694 606
Bsu36I CC'TnA_GG
Cuts at: 0 323 1160 2148
Size: 323 837 988
Fragments arranged by size:
988 837 323
BtgI C'CryG_G
Cuts at: 0 679 713 2148
Size: 679 34 1435
Fragments arranged by size:
1435 679 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 382 1092 2148
Size: 382 710 1056
Fragments arranged by size:
1056 710 382
BtsI GCAGTG_nn'
Cuts at: 0 56 1238 2148
Size: 56 1182 910
Fragments arranged by size:
1182 910 56
EaeI y'GGCC_r
Cuts at: 0 359 842 2148
Size: 359 483 1306
Fragments arranged by size:
1306 483 359
EarI CTCTTCn'nnn_
Cuts at: 0 1147 1579 1934 2148
Size: 1147 432 355 214
Fragments arranged by size:
1147 432 355 214
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 245 699 1243 2148
Size: 245 454 544 905
Fragments arranged by size:
905 544 454 245
EcoICRI GAG'CTC
Cuts at: 0 2055 2148
Size: 2055 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 313 633 790 874 1128 1158 1333
Size: 252 61 320 157 84 254 30 175
Cuts at: 1333 1486 1503 1945 2148
Size: 153 17 442 203
Fragments arranged by size:
442 320 254 252 203 175 157 153
84 61 30 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 949 1060 2148
Size: 949 111 1088
Fragments arranged by size:
1088 949 111
EcoO109I rG'GnC_Cy
Cuts at: 0 171 764 1179 1272 1290 1512 2031 2148
Size: 171 593 415 93 18 222 519 117
Fragments arranged by size:
593 519 415 222 171 117 93 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1668 2148
Size: 1668 480
HaeII r_GCGC'y
Cuts at: 0 479 1262 2148
Size: 479 783 886
Fragments arranged by size:
886 783 479
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 591 623 914 946 1578 1610 2148
Size: 591 32 291 32 632 32 538
Fragments arranged by size:
632 591 538 291 32 32 32
KasI G'GCGC_C
Cuts at: 0 1258 2148
Size: 1258 890
MscI TGG'CCA
Cuts at: 0 844 2148
Size: 844 1304
MslI CAynn'nnrTG
Cuts at: 0 32 622 1073 1342 2148
Size: 32 590 451 269 806
Fragments arranged by size:
806 590 451 269 32
MspA1I CmG'CkG
Cuts at: 0 804 828 2148
Size: 804 24 1320
Fragments arranged by size:
1320 804 24
NaeI GCC'GGC
Cuts at: 0 238 363 725 1263 2148
Size: 238 125 362 538 885
Fragments arranged by size:
885 538 362 238 125
NarI GG'CG_CC
Cuts at: 0 1259 2148
Size: 1259 889
NgoMIV G'CCGG_C
Cuts at: 0 236 361 723 1261 2148
Size: 236 125 362 538 887
Fragments arranged by size:
887 538 362 236 125
NruI TCG'CGA
Cuts at: 0 401 2148
Size: 401 1747
NspI r_CATG'y
Cuts at: 0 552 1361 2049 2148
Size: 552 809 688 99
Fragments arranged by size:
809 688 552 99
PasI CC'CwG_GG
Cuts at: 0 1905 2148
Size: 1905 243
PciI A'CATG_T
Cuts at: 0 548 2148
Size: 548 1600
PflMI CCAn_nnn'nTGG
Cuts at: 0 875 1696 2148
Size: 875 821 452
Fragments arranged by size:
875 821 452
PfoI T'CCnGG_A
Cuts at: 0 851 1004 1457 1775 1913 1934 2090 2148
Size: 851 153 453 318 138 21 156 58
Fragments arranged by size:
851 453 318 156 153 138 58 21
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 316 348 2148
Size: 316 32 1800
Fragments arranged by size:
1800 316 32
PpuMI rG'GwC_Cy
Cuts at: 0 171 1179 1512 2031 2148
Size: 171 1008 333 519 117
Fragments arranged by size:
1008 519 333 171 117
PspOMI G'GGCC_C
Cuts at: 0 763 1289 2148
Size: 763 526 859
Fragments arranged by size:
859 763 526
PspXI vC'TCGA_Gb
Cuts at: 0 1081 2148
Size: 1081 1067
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1642 1674 2148
Size: 1642 32 474
Fragments arranged by size:
1642 474 32
PstI C_TGCA'G
Cuts at: 0 821 920 1226 1479 1769 2148
Size: 821 99 306 253 290 379
Fragments arranged by size:
821 379 306 290 253 99
PvuII CAG'CTG
Cuts at: 0 804 828 2148
Size: 804 24 1320
Fragments arranged by size:
1320 804 24
SacI G_AGCT'C
Cuts at: 0 2057 2148
Size: 2057 91
SanDI GG'GwC_CC
Cuts at: 0 1179 2148
Size: 1179 969
SapI GCTCTTCn'nnn_
Cuts at: 0 1579 1934 2148
Size: 1579 355 214
Fragments arranged by size:
1579 355 214
SbfI CC_TGCA'GG
Cuts at: 0 1226 1769 2148
Size: 1226 543 379
Fragments arranged by size:
1226 543 379
ScaI AGT'ACT
Cuts at: 0 262 2148
Size: 262 1886
SfcI C'TryA_G
Cuts at: 0 817 916 1222 1475 1765 2148
Size: 817 99 306 253 290 383
Fragments arranged by size:
817 383 306 290 253 99
SfoI GGC'GCC
Cuts at: 0 1260 2148
Size: 1260 888
SmaI CCC'GGG
Cuts at: 0 484 2148
Size: 484 1664
SmlI C'TyrA_G
Cuts at: 0 1081 2148
Size: 1081 1067
StuI AGG'CCT
Cuts at: 0 1987 2148
Size: 1987 161
StyI C'CwwG_G
Cuts at: 0 1244 2148
Size: 1244 904
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 504 1982 2148
Size: 504 1478 166
Fragments arranged by size:
1478 504 166
TatI w'GTAC_w
Cuts at: 0 68 260 2148
Size: 68 192 1888
Fragments arranged by size:
1888 192 68
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 643 1251 2148
Size: 643 608 897
Fragments arranged by size:
897 643 608
XhoI C'TCGA_G
Cuts at: 0 1081 2148
Size: 1081 1067
XmaI C'CCGG_G
Cuts at: 0 482 2148
Size: 482 1666
XmnI GAAnn'nnTTC
Cuts at: 0 115 2148
Size: 115 2033
ZraI GAC'GTC
Cuts at: 0 1194 2148
Size: 1194 954
Enzymes that do cut:
AarI AatII AcuI AflIII AleI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BglI
Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I KasI MscI MslI MspA1I NaeI NarI NgoMIV
NruI NspI PasI PciI PflMI PfoI PpiI PpuMI
PspOMI PspXI PsrI PstI PvuII SacI SanDI SapI
SbfI ScaI SfcI SfoI SmaI SmlI StuI StyI
TaqII TatI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI BpuEI
BsaAI BsaBI BsiEI BsiWI BspHI BssHII BstAPI BstZ17I
ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI NcoI NdeI NheI NotI NsiI PacI
PmeI PmlI PshAI PsiI PvuI RsrII SacII SalI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI