Restriction map for
pF1KA0148
(Linear) (Six-base) MAPSORT of: KIAA0148.seq Check: 3965 from: 1 to: 2043
KIAA0148.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AatII G_ACGT'C
Cuts at: 0 1368 1879 2043
Size: 1368 511 164
Fragments arranged by size:
1368 511 164
Acc65I G'GTAC_C
Cuts at: 0 1632 2043
Size: 1632 411
AclI AA'CG_TT
Cuts at: 0 82 2043
Size: 82 1961
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 2043
Size: 166 1877
AflII C'TTAA_G
Cuts at: 0 1737 2043
Size: 1737 306
AflIII A'CryG_T
Cuts at: 0 1794 2043
Size: 1794 249
AhdI GACnn_n'nnGTC
Cuts at: 0 249 2043
Size: 249 1794
ApoI r'AATT_y
Cuts at: 0 313 1269 2043
Size: 313 956 774
Fragments arranged by size:
956 774 313
AvaI C'yCGr_G
Cuts at: 0 1092 1376 2043
Size: 1092 284 667
Fragments arranged by size:
1092 667 284
BamHI G'GATC_C
Cuts at: 0 51 2043
Size: 51 1992
BanI G'GyrC_C
Cuts at: 0 125 632 1632 2043
Size: 125 507 1000 411
Fragments arranged by size:
1000 507 411 125
BanII G_rGCy'C
Cuts at: 0 1118 1736 2043
Size: 1118 618 307
Fragments arranged by size:
1118 618 307
BbeI G_GCGC'C
Cuts at: 0 129 2043
Size: 129 1914
BbsI GAAGACnn'nnnn_
Cuts at: 0 273 1208 1708 1918 2043
Size: 273 935 500 210 125
Fragments arranged by size:
935 500 273 210 125
BciVI GTATCCnnnnn_n'
Cuts at: 0 1784 2043
Size: 1784 259
BglII A'GATC_T
Cuts at: 0 393 2043
Size: 393 1650
BlpI GC'TnA_GC
Cuts at: 0 769 2043
Size: 769 1274
Bme1580I G_kGCm'C
Cuts at: 0 635 2043
Size: 635 1408
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1243 1462 1528 2043
Size: 1243 219 66 515
Fragments arranged by size:
1243 515 219 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 187 1399 2043
Size: 187 1212 644
Fragments arranged by size:
1212 644 187
BsaAI yAC'GTr
Cuts at: 0 1797 2043
Size: 1797 246
BsaHI Gr'CG_yC
Cuts at: 0 126 1061 1365 1876 2043
Size: 126 935 304 511 167
Fragments arranged by size:
935 511 304 167 126
BsaWI w'CCGG_w
Cuts at: 0 14 957 1408 2043
Size: 14 943 451 635
Fragments arranged by size:
943 635 451 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1415 1445 2043
Size: 1415 30 598
Fragments arranged by size:
1415 598 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1083 2043
Size: 1083 960
BsiEI CG_ry'CG
Cuts at: 0 944 2043
Size: 944 1099
BsiHKAI G_wGCw'C
Cuts at: 0 471 1118 1736 2043
Size: 471 647 618 307
Fragments arranged by size:
647 618 471 307
BsiWI C'GTAC_G
Cuts at: 0 1982 2043
Size: 1982 61
BsmI GAATG_Cn'
Cuts at: 0 231 2043
Size: 231 1812
BsmBI CGTCTCn'nnnn_
Cuts at: 0 877 1356 2043
Size: 877 479 687
Fragments arranged by size:
877 687 479
Bsp1286I G_dGCh'C
Cuts at: 0 471 635 1118 1736 2043
Size: 471 164 483 618 307
Fragments arranged by size:
618 483 471 307 164
BspEI T'CCGG_A
Cuts at: 0 14 2043
Size: 14 2029
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1956 2043
Size: 1956 87
BsrFI r'CCGG_y
Cuts at: 0 1238 2043
Size: 1238 805
BssSI C'ACGA_G
Cuts at: 0 655 2043
Size: 655 1388
BstAPI GCAn_nnn'nTGC
Cuts at: 0 237 799 2043
Size: 237 562 1244
Fragments arranged by size:
1244 562 237
BstBI TT'CG_AA
Cuts at: 0 1273 2043
Size: 1273 770
BstEII G'GTnAC_C
Cuts at: 0 927 2013 2043
Size: 927 1086 30
Fragments arranged by size:
1086 927 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1560 2010 2043
Size: 1560 450 33
Fragments arranged by size:
1560 450 33
BstYI r'GATC_y
Cuts at: 0 51 393 2043
Size: 51 342 1650
Fragments arranged by size:
1650 342 51
Bsu36I CC'TnA_GG
Cuts at: 0 1279 2043
Size: 1279 764
BtgI C'CryG_G
Cuts at: 0 163 851 2043
Size: 163 688 1192
Fragments arranged by size:
1192 688 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 337 1974 2043
Size: 337 1637 69
Fragments arranged by size:
1637 337 69
BtsI GCAGTG_nn'
Cuts at: 0 111 175 1220 2043
Size: 111 64 1045 823
Fragments arranged by size:
1045 823 111 64
DraIII CAC_nnn'GTG
Cuts at: 0 164 2043
Size: 164 1879
EaeI y'GGCC_r
Cuts at: 0 1615 2043
Size: 1615 428
EarI CTCTTCn'nnn_
Cuts at: 0 1356 1454 2043
Size: 1356 98 589
Fragments arranged by size:
1356 589 98
EcoICRI GAG'CTC
Cuts at: 0 1116 1734 2043
Size: 1116 618 309
Fragments arranged by size:
1116 618 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 1243 1462 1528 2043
Size: 166 1077 219 66 515
Fragments arranged by size:
1077 515 219 166 66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 494 2043
Size: 494 1549
EcoO109I rG'GnC_Cy
Cuts at: 0 1926 2043
Size: 1926 117
EcoRI G'AATT_C
Cuts at: 0 313 2043
Size: 313 1730
HaeII r_GCGC'y
Cuts at: 0 129 654 923 2043
Size: 129 525 269 1120
Fragments arranged by size:
1120 525 269 129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 235 267 961 993 1396 1428 1918 1950
Size: 235 32 694 32 403 32 490 32
Cuts at: 1950 2043
Size: 93
Fragments arranged by size:
694 490 403 235 93 32 32 32
32
HincII GTy'rAC
Cuts at: 0 873 2043
Size: 873 1170
HindIII A'AGCT_T
Cuts at: 0 1246 2043
Size: 1246 797
KasI G'GCGC_C
Cuts at: 0 125 2043
Size: 125 1918
KpnI G_GTAC'C
Cuts at: 0 1636 2043
Size: 1636 407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 1367 2043
Size: 196 1171 676
Fragments arranged by size:
1171 676 196
MscI TGG'CCA
Cuts at: 0 1617 2043
Size: 1617 426
MslI CAynn'nnrTG
Cuts at: 0 725 856 1396 2043
Size: 725 131 540 647
Fragments arranged by size:
725 647 540 131
MspA1I CmG'CkG
Cuts at: 0 44 1953 2010 2043
Size: 44 1909 57 33
Fragments arranged by size:
1909 57 44 33
NarI GG'CG_CC
Cuts at: 0 126 2043
Size: 126 1917
NcoI C'CATG_G
Cuts at: 0 851 2043
Size: 851 1192
PasI CC'CwG_GG
Cuts at: 0 1921 2043
Size: 1921 122
PflMI CCAn_nnn'nTGG
Cuts at: 0 642 1624 2043
Size: 642 982 419
Fragments arranged by size:
982 642 419
PpuMI rG'GwC_Cy
Cuts at: 0 1926 2043
Size: 1926 117
PshAI GACnn'nnGTC
Cuts at: 0 940 1368 1896 2043
Size: 940 428 528 147
Fragments arranged by size:
940 528 428 147
PsiI TTA'TAA
Cuts at: 0 742 2043
Size: 742 1301
PstI C_TGCA'G
Cuts at: 0 43 106 1898 2043
Size: 43 63 1792 145
Fragments arranged by size:
1792 145 63 43
PvuII CAG'CTG
Cuts at: 0 1953 2010 2043
Size: 1953 57 33
Fragments arranged by size:
1953 57 33
SacI G_AGCT'C
Cuts at: 0 1118 1736 2043
Size: 1118 618 307
Fragments arranged by size:
1118 618 307
SanDI GG'GwC_CC
Cuts at: 0 1926 2043
Size: 1926 117
ScaI AGT'ACT
Cuts at: 0 973 2043
Size: 973 1070
SfcI C'TryA_G
Cuts at: 0 39 102 1894 2043
Size: 39 63 1792 149
Fragments arranged by size:
1792 149 63 39
SfoI GGC'GCC
Cuts at: 0 127 2043
Size: 127 1916
SmaI CCC'GGG
Cuts at: 0 1378 2043
Size: 1378 665
SmlI C'TyrA_G
Cuts at: 0 1737 2043
Size: 1737 306
StyI C'CwwG_G
Cuts at: 0 851 1994 2043
Size: 851 1143 49
Fragments arranged by size:
1143 851 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1953 2043
Size: 1953 90
TatI w'GTAC_w
Cuts at: 0 294 971 1328 2043
Size: 294 677 357 715
Fragments arranged by size:
715 677 357 294
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1845 2043
Size: 1845 198
XmaI C'CCGG_G
Cuts at: 0 1376 2043
Size: 1376 667
ZraI GAC'GTC
Cuts at: 0 1366 1877 2043
Size: 1366 511 166
Fragments arranged by size:
1366 511 166
Enzymes that do cut:
AatII Acc65I AclI AcuI AflII AflIII AhdI ApoI
AvaI BamHI BanI BanII BbeI BbsI BciVI BglII
BlpI Bme1580I BpmI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspMI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII HindIII
KasI KpnI MmeI MscI MslI MspA1I NarI NcoI
PasI PflMI PpuMI PshAI PsiI PstI PvuII SacI
SanDI ScaI SfcI SfoI SmaI SmlI StyI TaqII
TatI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI AfeI AgeI AleI AlfI AloI AlwNI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbvCI
BcgI BclI BfrBI BglI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaBI BseYI BsgI BspHI BsrBI BsrDI
BsrGI BssHII BstZ17I ClaI CspCI DraI DrdI EagI
EciI EcoRV FalI FseI FspI FspAI HpaI MfeI
MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PmlI PpiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SapI SbfI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XhoI XmnI