Restriction map for
pF1KSDA0140
(Linear) (Six-base) MAPSORT of: KIAA0140.seq Check: 2263 from: 1 to: 1266
KIAA0140.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 610 756 1266
Size: 610 146 510
Fragments arranged by size:
610 510 146
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 157 459 486 875 903 1266
Size: 48 109 302 27 389 28 363
Fragments arranged by size:
389 363 302 109 48 28 27
AfeI AGC'GCT
Cuts at: 0 460 1266
Size: 460 806
AlwNI CAG_nnn'CTG
Cuts at: 0 200 1004 1220 1266
Size: 200 804 216 46
Fragments arranged by size:
804 216 200 46
ApaI G_GGCC'C
Cuts at: 0 1206 1266
Size: 1206 60
AvaI C'yCGr_G
Cuts at: 0 32 515 1266
Size: 32 483 751
Fragments arranged by size:
751 483 32
BanI G'GyrC_C
Cuts at: 0 236 591 848 1266
Size: 236 355 257 418
Fragments arranged by size:
418 355 257 236
BanII G_rGCy'C
Cuts at: 0 403 637 973 1206 1266
Size: 403 234 336 233 60
Fragments arranged by size:
403 336 234 233 60
BbeI G_GCGC'C
Cuts at: 0 852 1266
Size: 852 414
BbvCI CC'TCA_GC
Cuts at: 0 25 286 315 732 928 1266
Size: 25 261 29 417 196 338
Fragments arranged by size:
417 338 261 196 29 25
BclI T'GATC_A
Cuts at: 0 275 1266
Size: 275 991
BglI GCCn_nnn'nGGC
Cuts at: 0 898 1037 1266
Size: 898 139 229
Fragments arranged by size:
898 229 139
BlpI GC'TnA_GC
Cuts at: 0 600 1266
Size: 600 666
Bme1580I G_kGCm'C
Cuts at: 0 1206 1266
Size: 1206 60
BmgBI CAC'GTC
Cuts at: 0 987 1266
Size: 987 279
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 896 1266
Size: 896 370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 651 762 1266
Size: 651 111 504
Fragments arranged by size:
651 504 111
Bpu10I CC'TnA_GC
Cuts at: 0 25 286 315 732 928 937 1067 1266
Size: 25 261 29 417 196 9 130 199
Fragments arranged by size:
417 261 199 196 130 29 25 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 143 1266
Size: 143 1123
BsaHI Gr'CG_yC
Cuts at: 0 849 1266
Size: 849 417
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 514 544 1102 1132 1266
Size: 514 30 558 30 134
Fragments arranged by size:
558 514 134 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 504 522 671 1141 1266
Size: 504 18 149 470 125
Fragments arranged by size:
504 470 149 125 18
BseYI C'CCAG_C
Cuts at: 0 803 1051 1266
Size: 803 248 215
Fragments arranged by size:
803 248 215
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 628 1266
Size: 61 567 638
Fragments arranged by size:
638 567 61
BsiHKAI G_wGCw'C
Cuts at: 0 193 530 1266
Size: 193 337 736
Fragments arranged by size:
736 337 193
BsmI GAATG_Cn'
Cuts at: 0 211 1266
Size: 211 1055
BsmBI CGTCTCn'nnnn_
Cuts at: 0 423 765 1266
Size: 423 342 501
Fragments arranged by size:
501 423 342
Bsp1286I G_dGCh'C
Cuts at: 0 193 403 530 637 973 1206 1266
Size: 193 210 127 107 336 233 60
Fragments arranged by size:
336 233 210 193 127 107 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 610 650 680 756 1266
Size: 610 40 30 76 510
Fragments arranged by size:
610 510 76 40 30
BsrBI CCG'CTC
Cuts at: 0 344 800 1171 1266
Size: 344 456 371 95
Fragments arranged by size:
456 371 344 95
BsrDI GCAATG_nn'
Cuts at: 0 47 1266
Size: 47 1219
BsrFI r'CCGG_y
Cuts at: 0 662 1176 1266
Size: 662 514 90
Fragments arranged by size:
662 514 90
BstXI CCAn_nnnn'nTGG
Cuts at: 0 568 1266
Size: 568 698
BtgI C'CryG_G
Cuts at: 0 419 650 1197 1266
Size: 419 231 547 69
Fragments arranged by size:
547 419 231 69
BtsI GCAGTG_nn'
Cuts at: 0 254 1266
Size: 254 1012
DraIII CAC_nnn'GTG
Cuts at: 0 651 1266
Size: 651 615
DrdI GACnn_nn'nnGTC
Cuts at: 0 829 1099 1266
Size: 829 270 167
Fragments arranged by size:
829 270 167
EarI CTCTTCn'nnn_
Cuts at: 0 176 1079 1266
Size: 176 903 187
Fragments arranged by size:
903 187 176
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 157 459 486 651 762 875 903
Size: 48 109 302 27 165 111 113 28
Cuts at: 903 1266
Size: 363
Fragments arranged by size:
363 302 165 113 111 109 48 28
27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1150 1266
Size: 1150 116
EcoO109I rG'GnC_Cy
Cuts at: 0 59 103 392 1001 1043 1055 1113 1202
Size: 59 44 289 609 42 12 58 89
Cuts at: 1202 1203 1266
Size: 1 63
Fragments arranged by size:
609 289 89 63 59 58 44 42
12 1
HaeII r_GCGC'y
Cuts at: 0 336 462 852 1266
Size: 336 126 390 414
Fragments arranged by size:
414 390 336 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 352 384 1102 1134 1266
Size: 352 32 718 32 132
Fragments arranged by size:
718 352 132 32 32
KasI G'GCGC_C
Cuts at: 0 848 1266
Size: 848 418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 1220 1266
Size: 491 729 46
Fragments arranged by size:
729 491 46
MspA1I CmG'CkG
Cuts at: 0 443 678 782 932 1051 1139 1199 1266
Size: 443 235 104 150 119 88 60 67
Fragments arranged by size:
443 235 150 119 104 88 67 60
NaeI GCC'GGC
Cuts at: 0 664 1178 1266
Size: 664 514 88
Fragments arranged by size:
664 514 88
NarI GG'CG_CC
Cuts at: 0 849 1266
Size: 849 417
NgoMIV G'CCGG_C
Cuts at: 0 662 1176 1266
Size: 662 514 90
Fragments arranged by size:
662 514 90
NspI r_CATG'y
Cuts at: 0 56 1266
Size: 56 1210
PasI CC'CwG_GG
Cuts at: 0 1033 1207 1266
Size: 1033 174 59
Fragments arranged by size:
1033 174 59
PflMI CCAn_nnn'nTGG
Cuts at: 0 200 410 1004 1266
Size: 200 210 594 262
Fragments arranged by size:
594 262 210 200
PpuMI rG'GwC_Cy
Cuts at: 0 59 103 1043 1055 1113 1266
Size: 59 44 940 12 58 153
Fragments arranged by size:
940 153 59 58 44 12
PshAI GACnn'nnGTC
Cuts at: 0 959 1060 1266
Size: 959 101 206
Fragments arranged by size:
959 206 101
PspOMI G'GGCC_C
Cuts at: 0 1202 1266
Size: 1202 64
PstI C_TGCA'G
Cuts at: 0 677 1185 1266
Size: 677 508 81
Fragments arranged by size:
677 508 81
PvuII CAG'CTG
Cuts at: 0 932 1139 1266
Size: 932 207 127
Fragments arranged by size:
932 207 127
SacII CC_GC'GG
Cuts at: 0 1200 1266
Size: 1200 66
SanDI GG'GwC_CC
Cuts at: 0 1055 1266
Size: 1055 211
SfcI C'TryA_G
Cuts at: 0 438 673 1181 1266
Size: 438 235 508 85
Fragments arranged by size:
508 438 235 85
SfoI GGC'GCC
Cuts at: 0 850 1266
Size: 850 416
SmaI CCC'GGG
Cuts at: 0 34 517 1266
Size: 34 483 749
Fragments arranged by size:
749 483 34
StuI AGG'CCT
Cuts at: 0 876 1266
Size: 876 390
StyI C'CwwG_G
Cuts at: 0 395 585 1266
Size: 395 190 681
Fragments arranged by size:
681 395 190
XbaI T'CTAG_A
Cuts at: 0 882 1266
Size: 882 384
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 565 1266
Size: 565 701
XmaI C'CCGG_G
Cuts at: 0 32 515 1266
Size: 32 483 751
Fragments arranged by size:
751 483 32
Enzymes that do cut:
AarI AcuI AfeI AlwNI ApaI AvaI BanI BanII
BbeI BbvCI BclI BglI BlpI Bme1580I BmgBI BplI
BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BstXI BtgI BtsI DraIII DrdI EarI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI MmeI MspA1I NaeI NarI
NgoMIV NspI PasI PflMI PpuMI PshAI PspOMI PstI
PvuII SacII SanDI SfcI SfoI SmaI StuI StyI
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BcgI BciVI BfrBI BglII
BmrI BmtI BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI
BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI EaeI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MscI
MslI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PsiI PspXI PsrI
PvuI RsrII SacI SalI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TatI TstI Tth111I XhoI XmnI
ZraI