Restriction map for
pF1KSDA0137
(Linear) (Six-base) MAPSORT of: KIAA0137.seq Check: 799 from: 1 to: 2298
KIAA0137.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AclI AA'CG_TT
Cuts at: 0 1266 2298
Size: 1266 1032
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1669 2213 2298
Size: 1669 544 85
Fragments arranged by size:
1669 544 85
AflII C'TTAA_G
Cuts at: 0 1759 2298
Size: 1759 539
AgeI A'CCGG_T
Cuts at: 0 2100 2298
Size: 2100 198
AleI CACnn'nnGTG
Cuts at: 0 1295 2298
Size: 1295 1003
AlwNI CAG_nnn'CTG
Cuts at: 0 693 2298
Size: 693 1605
ApaLI G'TGCA_C
Cuts at: 0 2173 2298
Size: 2173 125
ApoI r'AATT_y
Cuts at: 0 516 1155 1202 2223 2298
Size: 516 639 47 1021 75
Fragments arranged by size:
1021 639 516 75 47
AvaI C'yCGr_G
Cuts at: 0 72 898 2298
Size: 72 826 1400
Fragments arranged by size:
1400 826 72
BamHI G'GATC_C
Cuts at: 0 165 2298
Size: 165 2133
BanI G'GyrC_C
Cuts at: 0 1074 2298
Size: 1074 1224
BanII G_rGCy'C
Cuts at: 0 79 2298
Size: 79 2219
BciVI GTATCCnnnnn_n'
Cuts at: 0 1581 2298
Size: 1581 717
Bme1580I G_kGCm'C
Cuts at: 0 905 2177 2298
Size: 905 1272 121
Fragments arranged by size:
1272 905 121
BmgBI CAC'GTC
Cuts at: 0 62 424 2298
Size: 62 362 1874
Fragments arranged by size:
1874 362 62
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 1497 2298
Size: 224 1273 801
Fragments arranged by size:
1273 801 224
Bpu10I CC'TnA_GC
Cuts at: 0 727 2298
Size: 727 1571
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 456 2298
Size: 456 1842
BsaAI yAC'GTr
Cuts at: 0 1297 2298
Size: 1297 1001
BsaBI GATnn'nnATC
Cuts at: 0 1643 2298
Size: 1643 655
BsaWI w'CCGG_w
Cuts at: 0 333 2100 2298
Size: 333 1767 198
Fragments arranged by size:
1767 333 198
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 500 2298
Size: 500 1798
BseYI C'CCAG_C
Cuts at: 0 50 2248 2298
Size: 50 2198 50
Fragments arranged by size:
2198 50 50
BsiEI CG_ry'CG
Cuts at: 0 556 2298
Size: 556 1742
BsiHKAI G_wGCw'C
Cuts at: 0 223 2177 2298
Size: 223 1954 121
Fragments arranged by size:
1954 223 121
BsmBI CGTCTCn'nnnn_
Cuts at: 0 68 2298
Size: 68 2230
Bsp1286I G_dGCh'C
Cuts at: 0 79 223 905 2177 2298
Size: 79 144 682 1272 121
Fragments arranged by size:
1272 682 144 121 79
BspHI T'CATG_A
Cuts at: 0 1188 2298
Size: 1188 1110
BsrDI GCAATG_nn'
Cuts at: 0 149 1635 2298
Size: 149 1486 663
Fragments arranged by size:
1486 663 149
BsrFI r'CCGG_y
Cuts at: 0 2100 2298
Size: 2100 198
BsrGI T'GTAC_A
Cuts at: 0 603 1603 2298
Size: 603 1000 695
Fragments arranged by size:
1000 695 603
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1839 2298
Size: 1839 459
BstYI r'GATC_y
Cuts at: 0 165 304 1872 2298
Size: 165 139 1568 426
Fragments arranged by size:
1568 426 165 139
BtsI GCAGTG_nn'
Cuts at: 0 24 249 2118 2298
Size: 24 225 1869 180
Fragments arranged by size:
1869 225 180 24
ClaI AT'CG_AT
Cuts at: 0 888 2163 2298
Size: 888 1275 135
Fragments arranged by size:
1275 888 135
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1151 1186 2298
Size: 1151 35 1112
Fragments arranged by size:
1151 1112 35
DraI TTT'AAA
Cuts at: 0 627 2298
Size: 627 1671
EaeI y'GGCC_r
Cuts at: 0 89 426 2298
Size: 89 337 1872
Fragments arranged by size:
1872 337 89
EarI CTCTTCn'nnn_
Cuts at: 0 868 1232 2298
Size: 868 364 1066
Fragments arranged by size:
1066 868 364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 1497 1669 2213 2298
Size: 224 1273 172 544 85
Fragments arranged by size:
1273 544 224 172 85
EcoO109I rG'GnC_Cy
Cuts at: 0 95 690 2298
Size: 95 595 1608
Fragments arranged by size:
1608 595 95
EcoRV GAT'ATC
Cuts at: 0 1720 2298
Size: 1720 578
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2065 2298
Size: 2065 233
HaeII r_GCGC'y
Cuts at: 0 929 2298
Size: 929 1369
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 307 339 881 913 1564 1596 1957 1989
Size: 307 32 542 32 651 32 361 32
Cuts at: 1989 2298
Size: 309
Fragments arranged by size:
651 542 361 309 307 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1172 2298
Size: 1172 1126
HindIII A'AGCT_T
Cuts at: 0 297 2298
Size: 297 2001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 228 1911 1959 1979 2298
Size: 91 137 1683 48 20 319
Fragments arranged by size:
1683 319 137 91 48 20
MscI TGG'CCA
Cuts at: 0 428 2298
Size: 428 1870
MslI CAynn'nnrTG
Cuts at: 0 814 1295 1508 2298
Size: 814 481 213 790
Fragments arranged by size:
814 790 481 213
MspA1I CmG'CkG
Cuts at: 0 2181 2298
Size: 2181 117
NspI r_CATG'y
Cuts at: 0 1513 1799 2298
Size: 1513 286 499
Fragments arranged by size:
1513 499 286
PasI CC'CwG_GG
Cuts at: 0 131 1884 2298
Size: 131 1753 414
Fragments arranged by size:
1753 414 131
PflMI CCAn_nnn'nTGG
Cuts at: 0 693 2298
Size: 693 1605
PfoI T'CCnGG_A
Cuts at: 0 121 686 2298
Size: 121 565 1612
Fragments arranged by size:
1612 565 121
PpuMI rG'GwC_Cy
Cuts at: 0 95 690 2298
Size: 95 595 1608
Fragments arranged by size:
1608 595 95
PsiI TTA'TAA
Cuts at: 0 2131 2298
Size: 2131 167
PstI C_TGCA'G
Cuts at: 0 1519 2298
Size: 1519 779
PvuII CAG'CTG
Cuts at: 0 2181 2298
Size: 2181 117
SfcI C'TryA_G
Cuts at: 0 1515 2298
Size: 1515 783
SmaI CCC'GGG
Cuts at: 0 74 2298
Size: 74 2224
SmlI C'TyrA_G
Cuts at: 0 471 1759 2298
Size: 471 1288 539
Fragments arranged by size:
1288 539 471
SspI AAT'ATT
Cuts at: 0 2066 2298
Size: 2066 232
StyI C'CwwG_G
Cuts at: 0 1583 2120 2298
Size: 1583 537 178
Fragments arranged by size:
1583 537 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 570 981 1361 1673 2298
Size: 570 411 380 312 625
Fragments arranged by size:
625 570 411 380 312
TatI w'GTAC_w
Cuts at: 0 603 844 1603 2298
Size: 603 241 759 695
Fragments arranged by size:
759 695 603 241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 16 463 2298
Size: 16 447 1835
Fragments arranged by size:
1835 447 16
XmaI C'CCGG_G
Cuts at: 0 72 2298
Size: 72 2226
Enzymes that do cut:
AclI AcuI AflII AgeI AleI AlwNI ApaLI ApoI
AvaI BamHI BanI BanII BciVI Bme1580I BmgBI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BsrDI BsrFI BsrGI BstXI
BstYI BtsI ClaI CspCI DraI EaeI EarI Eco57MI
EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII MmeI
MscI MslI MspA1I NspI PasI PflMI PfoI PpuMI
PsiI PstI PvuII SfcI SmaI SmlI SspI StyI
TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflIII AhdI AlfI
AloI ApaI AscI AseI AsiSI AvrII BaeI BbeI
BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI
BmrI BmtI BplI BsaI BsaHI BsaXI BsgI BsiWI
BsmI BspEI BspMI BsrBI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI FseI FspI FspAI HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
StuI SwaI TstI Tth111I XbaI XhoI XmnI ZraI