Restriction map for
pF1KSDA0135
(Linear) (Six-base) MAPSORT of: KIAA0135.seq Check: 6551 from: 1 to: 3990
KIAA0135.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AatII G_ACGT'C
Cuts at: 0 1232 3990
Size: 1232 2758
AccI GT'mk_AC
Cuts at: 0 2721 3990
Size: 2721 1269
Acc65I G'GTAC_C
Cuts at: 0 916 3990
Size: 916 3074
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 1667 3990
Size: 215 1452 2323
Fragments arranged by size:
2323 1452 215
AfeI AGC'GCT
Cuts at: 0 188 3990
Size: 188 3802
AflIII A'CryG_T
Cuts at: 0 788 2535 2570 3990
Size: 788 1747 35 1420
Fragments arranged by size:
1747 1420 788 35
AgeI A'CCGG_T
Cuts at: 0 976 3990
Size: 976 3014
AhdI GACnn_n'nnGTC
Cuts at: 0 2262 3990
Size: 2262 1728
AleI CACnn'nnGTG
Cuts at: 0 598 2623 3990
Size: 598 2025 1367
Fragments arranged by size:
2025 1367 598
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 284 1550 3990
Size: 284 1266 2440
Fragments arranged by size:
2440 1266 284
AlwNI CAG_nnn'CTG
Cuts at: 0 170 483 526 640 1512 2921 3727 3990
Size: 170 313 43 114 872 1409 806 263
Fragments arranged by size:
1409 872 806 313 263 170 114 43
ApaI G_GGCC'C
Cuts at: 0 85 2758 3922 3990
Size: 85 2673 1164 68
Fragments arranged by size:
2673 1164 85 68
ApaLI G'TGCA_C
Cuts at: 0 1495 3527 3990
Size: 1495 2032 463
Fragments arranged by size:
2032 1495 463
ApoI r'AATT_y
Cuts at: 0 2208 3990
Size: 2208 1782
AseI AT'TA_AT
Cuts at: 0 1316 3990
Size: 1316 2674
AvaI C'yCGr_G
Cuts at: 0 1349 3964 3990
Size: 1349 2615 26
Fragments arranged by size:
2615 1349 26
BamHI G'GATC_C
Cuts at: 0 1305 3126 3969 3990
Size: 1305 1821 843 21
Fragments arranged by size:
1821 1305 843 21
BanI G'GyrC_C
Cuts at: 0 742 916 2404 2674 3990
Size: 742 174 1488 270 1316
Fragments arranged by size:
1488 1316 742 270 174
BanII G_rGCy'C
Cuts at: 0 85 110 211 461 701 1127 1573 2022
Size: 85 25 101 250 240 426 446 449
Cuts at: 2022 2329 2750 2758 2882 2936 3010 3308 3922
Size: 307 421 8 124 54 74 298 614
Cuts at: 3922 3990
Size: 68
Fragments arranged by size:
614 449 446 426 421 307 298 250
240 124 101 85 74 68 54 25
8
BbsI GAAGACnn'nnnn_
Cuts at: 0 203 3990
Size: 203 3787
BbvCI CC'TCA_GC
Cuts at: 0 126 544 1292 1448 1664 2136 3431 3990
Size: 126 418 748 156 216 472 1295 559
Fragments arranged by size:
1295 748 559 472 418 216 156 126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1889 1923 3508 3542 3990
Size: 1889 34 1585 34 448
Fragments arranged by size:
1889 1585 448 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3364 3990
Size: 3364 626
BglI GCCn_nnn'nGGC
Cuts at: 0 2852 3014 3990
Size: 2852 162 976
Fragments arranged by size:
2852 976 162
BlpI GC'TnA_GC
Cuts at: 0 104 2876 3990
Size: 104 2772 1114
Fragments arranged by size:
2772 1114 104
Bme1580I G_kGCm'C
Cuts at: 0 85 1349 1499 2578 2758 3531 3922 3990
Size: 85 1264 150 1079 180 773 391 68
Fragments arranged by size:
1264 1079 773 391 180 150 85 68
BmgBI CAC'GTC
Cuts at: 0 752 804 3990
Size: 752 52 3186
Fragments arranged by size:
3186 752 52
BmrI ACTGGGnnnn_n'
Cuts at: 0 1995 3990
Size: 1995 1995
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 729 1444 2325 3990
Size: 729 715 881 1665
Fragments arranged by size:
1665 881 729 715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 1439 1442 1637 2335 2734 2974 3592
Size: 715 724 3 195 698 399 240 618
Cuts at: 3592 3658 3883 3990
Size: 66 225 107
Fragments arranged by size:
724 715 698 618 399 240 225 195
107 66 3
Bpu10I CC'TnA_GC
Cuts at: 0 126 142 278 544 1292 1448 1664 2136
Size: 126 16 136 266 748 156 216 472
Cuts at: 2136 3431 3902 3990
Size: 1295 471 88
Fragments arranged by size:
1295 748 472 471 266 216 156 136
126 88 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 2212 2497 3990
Size: 865 1347 285 1493
Fragments arranged by size:
1493 1347 865 285
BsaI GGTCTCn'nnnn_
Cuts at: 0 717 2099 3587 3637 3990
Size: 717 1382 1488 50 353
Fragments arranged by size:
1488 1382 717 353 50
BsaAI yAC'GTr
Cuts at: 0 789 2573 3990
Size: 789 1784 1417
Fragments arranged by size:
1784 1417 789
BsaHI Gr'CG_yC
Cuts at: 0 1229 3990
Size: 1229 2761
BsaWI w'CCGG_w
Cuts at: 0 976 1361 2884 3531 3990
Size: 976 385 1523 647 459
Fragments arranged by size:
1523 976 647 459 385
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1981 2011 3565 3595 3870 3900 3990
Size: 1981 30 1554 30 275 30 90
Fragments arranged by size:
1981 1554 275 90 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 565 979 2124 2602 2785 2944 3631 3655
Size: 565 414 1145 478 183 159 687 24
Cuts at: 3655 3990
Size: 335
Fragments arranged by size:
1145 687 565 478 414 335 183 159
24
BseYI C'CCAG_C
Cuts at: 0 84 109 808 955 1287 1659 1712 1741
Size: 84 25 699 147 332 372 53 29
Cuts at: 1741 1762 1882 1941 2890 3012 3990
Size: 21 120 59 949 122 978
Fragments arranged by size:
978 949 699 372 332 147 122 120
84 59 53 29 25 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 244 862 1011 2106 3990
Size: 74 170 618 149 1095 1884
Fragments arranged by size:
1884 1095 618 170 149 74
BsiEI CG_ry'CG
Cuts at: 0 1959 3285 3470 3990
Size: 1959 1326 185 520
Fragments arranged by size:
1959 1326 520 185
BsiHKAI G_wGCw'C
Cuts at: 0 211 284 557 1127 1499 2750 2882 2909
Size: 211 73 273 570 372 1251 132 27
Cuts at: 2909 3182 3531 3990
Size: 273 349 459
Fragments arranged by size:
1251 570 459 372 349 273 273 211
132 73 27
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2696 3726 3990
Size: 2696 1030 264
Fragments arranged by size:
2696 1030 264
Bsp1286I G_dGCh'C
Cuts at: 0 85 110 211 284 461 557 701 1127
Size: 85 25 101 73 177 96 144 426
Cuts at: 1127 1349 1499 1573 2022 2329 2578 2750 2758
Size: 222 150 74 449 307 249 172 8
Cuts at: 2758 2882 2909 2936 3010 3182 3308 3531 3922
Size: 124 27 27 74 172 126 223 391
Cuts at: 3922 3990
Size: 68
Fragments arranged by size:
449 426 391 307 249 223 222 177
172 172 150 144 126 124 101 96
85 74 74 73 68 27 27 25
8
BspEI T'CCGG_A
Cuts at: 0 1361 3990
Size: 1361 2629
BspHI T'CATG_A
Cuts at: 0 3692 3990
Size: 3692 298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 104 1589 1984 3383 3990
Size: 104 1485 395 1399 607
Fragments arranged by size:
1485 1399 607 395 104
BsrBI CCG'CTC
Cuts at: 0 998 1722 2074 3990
Size: 998 724 352 1916
Fragments arranged by size:
1916 998 724 352
BsrDI GCAATG_nn'
Cuts at: 0 1242 3990
Size: 1242 2748
BsrFI r'CCGG_y
Cuts at: 0 976 2401 3990
Size: 976 1425 1589
Fragments arranged by size:
1589 1425 976
BsrGI T'GTAC_A
Cuts at: 0 247 3599 3990
Size: 247 3352 391
Fragments arranged by size:
3352 391 247
BstEII G'GTnAC_C
Cuts at: 0 2610 3990
Size: 2610 1380
BstXI CCAn_nnnn'nTGG
Cuts at: 0 739 1224 2898 2946 3990
Size: 739 485 1674 48 1044
Fragments arranged by size:
1674 1044 739 485 48
BstYI r'GATC_y
Cuts at: 0 428 1305 1358 1581 2663 3126 3969 3990
Size: 428 877 53 223 1082 463 843 21
Fragments arranged by size:
1082 877 843 463 428 223 53 21
BstZ17I GTA'TAC
Cuts at: 0 2722 3990
Size: 2722 1268
Bsu36I CC'TnA_GG
Cuts at: 0 345 2564 3990
Size: 345 2219 1426
Fragments arranged by size:
2219 1426 345
BtgI C'CryG_G
Cuts at: 0 301 701 1071 1270 1772 1936 2156 3647
Size: 301 400 370 199 502 164 220 1491
Cuts at: 3647 3766 3990
Size: 119 224
Fragments arranged by size:
1491 502 400 370 301 224 220 199
164 119
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 752 3990
Size: 752 3238
BtsI GCAGTG_nn'
Cuts at: 0 98 261 315 1532 1683 1824 2189 2327
Size: 98 163 54 1217 151 141 365 138
Cuts at: 2327 3024 3371 3990
Size: 697 347 619
Fragments arranged by size:
1217 697 619 365 347 163 151 141
138 98 54
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1224 1259 3990
Size: 1224 35 2731
Fragments arranged by size:
2731 1224 35
DraIII CAC_nnn'GTG
Cuts at: 0 1344 3990
Size: 1344 2646
EaeI y'GGCC_r
Cuts at: 0 489 570 858 1214 1335 1775 2153 2421
Size: 489 81 288 356 121 440 378 268
Cuts at: 2421 3467 3945 3990
Size: 1046 478 45
Fragments arranged by size:
1046 489 478 440 378 356 288 268
121 81 45
EagI C'GGCC_G
Cuts at: 0 3467 3990
Size: 3467 523
EarI CTCTTCn'nnn_
Cuts at: 0 23 646 897 1958 2316 3274 3803 3990
Size: 23 623 251 1061 358 958 529 187
Fragments arranged by size:
1061 958 623 529 358 251 187 23
EcoICRI GAG'CTC
Cuts at: 0 209 1125 2748 2880 3990
Size: 209 916 1623 132 1110
Fragments arranged by size:
1623 1110 916 209 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 715 1439 1442 1637 1667 2335 2734
Size: 215 500 724 3 195 30 668 399
Cuts at: 2734 2974 3592 3658 3883 3990
Size: 240 618 66 225 107
Fragments arranged by size:
724 668 618 500 399 240 225 215
195 107 66 30 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 547 1457 3990
Size: 547 910 2533
Fragments arranged by size:
2533 910 547
EcoO109I rG'GnC_Cy
Cuts at: 0 81 480 540 1284 2172 2754 2755 2825
Size: 81 399 60 744 888 582 1 70
Cuts at: 2825 3023 3918 3919 3990
Size: 198 895 1 71
Fragments arranged by size:
895 888 744 582 399 198 81 71
70 60 1 1
EcoRI G'AATT_C
Cuts at: 0 2208 3990
Size: 2208 1782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 417 2253 3990
Size: 417 1836 1737
Fragments arranged by size:
1836 1737 417
HaeII r_GCGC'y
Cuts at: 0 190 672 1513 3990
Size: 190 482 841 2477
Fragments arranged by size:
2477 841 482 190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1343 1375 1908 1940 1981 2013 2248 2280
Size: 1343 32 533 32 41 32 235 32
Cuts at: 2280 3111 3143 3448 3480 3565 3567 3597 3599
Size: 831 32 305 32 85 2 30 2
Cuts at: 3599 3990
Size: 391
Fragments arranged by size:
1343 831 533 391 305 235 85 41
32 32 32 32 32 32 30 2
2
HincII GTy'rAC
Cuts at: 0 2415 3990
Size: 2415 1575
HindIII A'AGCT_T
Cuts at: 0 449 3990
Size: 449 3541
KpnI G_GTAC'C
Cuts at: 0 920 3990
Size: 920 3070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 879 2074 2488 2531 3097 3351 3990
Size: 879 1195 414 43 566 254 639
Fragments arranged by size:
1195 879 639 566 414 254 43
MscI TGG'CCA
Cuts at: 0 491 860 1216 1337 1777 2155 2423 3947
Size: 491 369 356 121 440 378 268 1524
Cuts at: 3947 3990
Size: 43
Fragments arranged by size:
1524 491 440 378 369 356 268 121
43
MslI CAynn'nnrTG
Cuts at: 0 598 2623 3990
Size: 598 2025 1367
Fragments arranged by size:
2025 1367 598
MspA1I CmG'CkG
Cuts at: 0 88 303 1220 1291 1663 1745 1800 1995
Size: 88 215 917 71 372 82 55 195
Cuts at: 1995 2658 2872 3012 3853 3990
Size: 663 214 140 841 137
Fragments arranged by size:
917 841 663 372 215 214 195 140
137 88 82 71 55
NspI r_CATG'y
Cuts at: 0 1692 3990
Size: 1692 2298
PasI CC'CwG_GG
Cuts at: 0 2167 3990
Size: 2167 1823
PflMI CCAn_nnn'nTGG
Cuts at: 0 2921 3838 3990
Size: 2921 917 152
Fragments arranged by size:
2921 917 152
PfoI T'CCnGG_A
Cuts at: 0 692 2218 2351 2372 2438 2666 2683 3990
Size: 692 1526 133 21 66 228 17 1307
Fragments arranged by size:
1526 1307 692 228 133 66 21 17
PmlI CAC'GTG
Cuts at: 0 2573 3990
Size: 2573 1417
PpuMI rG'GwC_Cy
Cuts at: 0 480 1284 2825 3990
Size: 480 804 1541 1165
Fragments arranged by size:
1541 1165 804 480
PshAI GACnn'nnGTC
Cuts at: 0 721 3990
Size: 721 3269
PspOMI G'GGCC_C
Cuts at: 0 81 2754 3918 3990
Size: 81 2673 1164 72
Fragments arranged by size:
2673 1164 81 72
PstI C_TGCA'G
Cuts at: 0 1973 2657 3719 3990
Size: 1973 684 1062 271
Fragments arranged by size:
1973 1062 684 271
PvuII CAG'CTG
Cuts at: 0 88 1220 1291 1663 1800 1995 3990
Size: 88 1132 71 372 137 195 1995
Fragments arranged by size:
1995 1132 372 195 137 88 71
SacI G_AGCT'C
Cuts at: 0 211 1127 2750 2882 3990
Size: 211 916 1623 132 1108
Fragments arranged by size:
1623 1108 916 211 132
SacII CC_GC'GG
Cuts at: 0 304 3990
Size: 304 3686
SapI GCTCTTCn'nnn_
Cuts at: 0 897 1958 3990
Size: 897 1061 2032
Fragments arranged by size:
2032 1061 897
ScaI AGT'ACT
Cuts at: 0 2983 3523 3990
Size: 2983 540 467
Fragments arranged by size:
2983 540 467
SexAI A'CCwGG_T
Cuts at: 0 3674 3990
Size: 3674 316
SfcI C'TryA_G
Cuts at: 0 1164 1969 2653 3555 3715 3990
Size: 1164 805 684 902 160 275
Fragments arranged by size:
1164 902 805 684 275 160
SgrAI Cr'CCGG_yG
Cuts at: 0 976 3990
Size: 976 3014
SmaI CCC'GGG
Cuts at: 0 3966 3990
Size: 3966 24
SmlI C'TyrA_G
Cuts at: 0 880 2227 2476 3990
Size: 880 1347 249 1514
Fragments arranged by size:
1514 1347 880 249
SspI AAT'ATT
Cuts at: 0 243 3990
Size: 243 3747
StuI AGG'CCT
Cuts at: 0 1904 3990
Size: 1904 2086
StyI C'CwwG_G
Cuts at: 0 1262 1309 1517 1538 1790 2150 2315 2418
Size: 1262 47 208 21 252 360 165 103
Cuts at: 2418 2424 2593 2939 3219 3743 3948 3990
Size: 6 169 346 280 524 205 42
Fragments arranged by size:
1262 524 360 346 280 252 208 205
169 165 103 47 42 21 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1008 1235 1973 2875 3990
Size: 1008 227 738 902 1115
Fragments arranged by size:
1115 1008 902 738 227
TatI w'GTAC_w
Cuts at: 0 247 2981 2993 3521 3599 3990
Size: 247 2734 12 528 78 391
Fragments arranged by size:
2734 528 391 247 78 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2527 2559 3799 3831 3870 3902 3990
Size: 2527 32 1240 32 39 32 88
Fragments arranged by size:
2527 1240 88 39 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1471 3990
Size: 1471 2519
XbaI T'CTAG_A
Cuts at: 0 1926 3990
Size: 1926 2064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 405 484 641 1797 3009 3990
Size: 405 79 157 1156 1212 981
Fragments arranged by size:
1212 1156 981 405 157 79
XmaI C'CCGG_G
Cuts at: 0 3964 3990
Size: 3964 26
XmnI GAAnn'nnTTC
Cuts at: 0 2045 3625 3990
Size: 2045 1580 365
Fragments arranged by size:
2045 1580 365
ZraI GAC'GTC
Cuts at: 0 1230 3990
Size: 1230 2760
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI DraIII EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII KpnI MmeI MscI MslI MspA1I NspI
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI
PvuII SacI SacII SapI ScaI SexAI SfcI SgrAI
SmaI SmlI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AflII AloI AscI AsiSI AvrII BaeI
BbeI BclI BfrBI BglII BmtI BsaBI BsiWI BsmI
BssHII BssSI BstAPI BstBI ClaI DraI DrdI EciI
EcoRV FseI FspI FspAI HpaI KasI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PpiI PsiI PspXI PsrI
PvuI RsrII SalI SanDI SbfI SfiI SfoI SgfI
SnaBI SpeI SphI SrfI SwaI XhoI