Restriction map for
pF1KSDA0127
(Linear) (Six-base) MAPSORT of: KIAA0127.seq Check: 2889 from: 1 to: 942
KIAA0127.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AatII G_ACGT'C
Cuts at: 0 700 942
Size: 700 242
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 942
Size: 303 639
AflIII A'CryG_T
Cuts at: 0 200 422 942
Size: 200 222 520
Fragments arranged by size:
520 222 200
ApoI r'AATT_y
Cuts at: 0 681 942
Size: 681 261
AvaI C'yCGr_G
Cuts at: 0 406 942
Size: 406 536
BamHI G'GATC_C
Cuts at: 0 212 942
Size: 212 730
BanI G'GyrC_C
Cuts at: 0 610 942
Size: 610 332
BanII G_rGCy'C
Cuts at: 0 170 287 536 942
Size: 170 117 249 406
Fragments arranged by size:
406 249 170 117
BglI GCCn_nnn'nGGC
Cuts at: 0 313 942
Size: 313 629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 461 582 942
Size: 397 64 121 360
Fragments arranged by size:
397 360 121 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 942
Size: 617 325
BsaHI Gr'CG_yC
Cuts at: 0 331 697 942
Size: 331 366 245
Fragments arranged by size:
366 331 245
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 490 520 834 864 942
Size: 490 30 314 30 78
Fragments arranged by size:
490 314 78 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 255 498 544 826 942
Size: 255 243 46 282 116
Fragments arranged by size:
282 255 243 116 46
BseYI C'CCAG_C
Cuts at: 0 169 269 286 942
Size: 169 100 17 656
Fragments arranged by size:
656 169 100 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 765 942
Size: 765 177
BsiHKAI G_wGCw'C
Cuts at: 0 536 942
Size: 536 406
Bsp1286I G_dGCh'C
Cuts at: 0 170 287 536 942
Size: 170 117 249 406
Fragments arranged by size:
406 249 170 117
BsrFI r'CCGG_y
Cuts at: 0 312 942
Size: 312 630
BstYI r'GATC_y
Cuts at: 0 212 942
Size: 212 730
Bsu36I CC'TnA_GG
Cuts at: 0 244 942
Size: 244 698
BtgI C'CryG_G
Cuts at: 0 452 566 701 942
Size: 452 114 135 241
Fragments arranged by size:
452 241 135 114
BtsI GCAGTG_nn'
Cuts at: 0 400 942
Size: 400 542
EaeI y'GGCC_r
Cuts at: 0 563 942
Size: 563 379
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 225 942
Size: 225 717
EcoICRI GAG'CTC
Cuts at: 0 534 942
Size: 534 408
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 397 461 582 942
Size: 303 94 64 121 360
Fragments arranged by size:
360 303 121 94 64
EcoO109I rG'GnC_Cy
Cuts at: 0 155 306 593 942
Size: 155 151 287 349
Fragments arranged by size:
349 287 155 151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 902 942
Size: 902 40
HaeII r_GCGC'y
Cuts at: 0 111 942
Size: 111 831
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 67 99 490 522 654 686 942
Size: 67 32 391 32 132 32 256
Fragments arranged by size:
391 256 132 67 32 32 32
MslI CAynn'nnrTG
Cuts at: 0 41 925 942
Size: 41 884 17
Fragments arranged by size:
884 41 17
NaeI GCC'GGC
Cuts at: 0 314 942
Size: 314 628
NgoMIV G'CCGG_C
Cuts at: 0 312 942
Size: 312 630
NspI r_CATG'y
Cuts at: 0 204 942
Size: 204 738
PacI TTA_AT'TAA
Cuts at: 0 194 942
Size: 194 748
PciI A'CATG_T
Cuts at: 0 200 942
Size: 200 742
PflMI CCAn_nnn'nTGG
Cuts at: 0 921 942
Size: 921 21
PfoI T'CCnGG_A
Cuts at: 0 215 942
Size: 215 727
PpuMI rG'GwC_Cy
Cuts at: 0 593 942
Size: 593 349
PspXI vC'TCGA_Gb
Cuts at: 0 406 942
Size: 406 536
SacI G_AGCT'C
Cuts at: 0 536 942
Size: 536 406
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 313 942
Size: 313 629
SmlI C'TyrA_G
Cuts at: 0 406 632 942
Size: 406 226 310
Fragments arranged by size:
406 310 226
StuI AGG'CCT
Cuts at: 0 382 942
Size: 382 560
StyI C'CwwG_G
Cuts at: 0 86 515 942
Size: 86 429 427
Fragments arranged by size:
429 427 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 628 942
Size: 628 314
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 316 348 942
Size: 316 32 594
Fragments arranged by size:
594 316 32
XhoI C'TCGA_G
Cuts at: 0 406 942
Size: 406 536
ZraI GAC'GTC
Cuts at: 0 698 942
Size: 698 244
Enzymes that do cut:
AatII AcuI AflIII ApoI AvaI BamHI BanI BanII
BglI BpmI BpuEI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BsrFI BstYI Bsu36I BtgI BtsI EaeI
EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I MslI
NaeI NgoMIV NspI PacI PciI PflMI PfoI PpuMI
PspXI SacI SfiI SmlI StuI StyI TaqII TstI
XhoI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EarI EcoNI
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MspA1I NarI
NcoI NdeI NheI NotI NruI NsiI PasI PmeI
PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XcmI
XmaI XmnI