Restriction map for
pF1KSDA0126
(Linear) (Six-base) MAPSORT of: KIAA0126.seq Check: 3425 from: 1 to: 3219
KIAA0126.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1773 3219
Size: 1773 1446
Acc65I G'GTAC_C
Cuts at: 0 2584 3219
Size: 2584 635
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 1195 1369 2706 3219
Size: 127 1068 174 1337 513
Fragments arranged by size:
1337 1068 513 174 127
AflIII A'CryG_T
Cuts at: 0 2139 2282 3219
Size: 2139 143 937
Fragments arranged by size:
2139 937 143
AleI CACnn'nnGTG
Cuts at: 0 3021 3219
Size: 3021 198
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 618 1265 3219
Size: 618 647 1954
Fragments arranged by size:
1954 647 618
AlwNI CAG_nnn'CTG
Cuts at: 0 1173 3219
Size: 1173 2046
ApoI r'AATT_y
Cuts at: 0 178 1897 2209 2343 2728 2799 3219
Size: 178 1719 312 134 385 71 420
Fragments arranged by size:
1719 420 385 312 178 134 71
BamHI G'GATC_C
Cuts at: 0 3000 3219
Size: 3000 219
BanI G'GyrC_C
Cuts at: 0 2584 2701 3219
Size: 2584 117 518
Fragments arranged by size:
2584 518 117
BanII G_rGCy'C
Cuts at: 0 720 875 1834 3219
Size: 720 155 959 1385
Fragments arranged by size:
1385 959 720 155
BbsI GAAGACnn'nnnn_
Cuts at: 0 1006 3219
Size: 1006 2213
BciVI GTATCCnnnnn_n'
Cuts at: 0 2127 2276 3219
Size: 2127 149 943
Fragments arranged by size:
2127 943 149
BclI T'GATC_A
Cuts at: 0 1599 3219
Size: 1599 1620
BfrBI ATG'CAT
Cuts at: 0 895 3219
Size: 895 2324
BglII A'GATC_T
Cuts at: 0 653 737 864 1160 3219
Size: 653 84 127 296 2059
Fragments arranged by size:
2059 653 296 127 84
BlpI GC'TnA_GC
Cuts at: 0 1658 2642 3219
Size: 1658 984 577
Fragments arranged by size:
1658 984 577
Bme1580I G_kGCm'C
Cuts at: 0 1419 2819 3219
Size: 1419 1400 400
Fragments arranged by size:
1419 1400 400
BmtI G_CTAG'C
Cuts at: 0 137 3219
Size: 137 3082
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1171 1181 2002 2480 3219
Size: 1171 10 821 478 739
Fragments arranged by size:
1171 821 739 478 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 1405 3219
Size: 15 1390 1814
Fragments arranged by size:
1814 1390 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 2213 3219
Size: 2213 1006
BsaBI GATnn'nnATC
Cuts at: 0 1717 2300 3219
Size: 1717 583 919
Fragments arranged by size:
1717 919 583
BsaWI w'CCGG_w
Cuts at: 0 2132 3219
Size: 2132 1087
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 517 547 1501 1531 2357 2387 3219
Size: 517 30 954 30 826 30 832
Fragments arranged by size:
954 832 826 517 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1381 2809 3219
Size: 1381 1428 410
Fragments arranged by size:
1428 1381 410
BseYI C'CCAG_C
Cuts at: 0 129 301 1008 1497 2744 3219
Size: 129 172 707 489 1247 475
Fragments arranged by size:
1247 707 489 475 172 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 624 1525 2751 3219
Size: 619 5 901 1226 468
Fragments arranged by size:
1226 901 619 468 5
BsiHKAI G_wGCw'C
Cuts at: 0 875 3016 3219
Size: 875 2141 203
Fragments arranged by size:
2141 875 203
Bsp1286I G_dGCh'C
Cuts at: 0 720 875 1419 1834 2819 3016 3219
Size: 720 155 544 415 985 197 203
Fragments arranged by size:
985 720 544 415 203 197 155
BspHI T'CATG_A
Cuts at: 0 824 3219
Size: 824 2395
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 590 1629 1773 2396 3219
Size: 590 1039 144 623 823
Fragments arranged by size:
1039 823 623 590 144
BsrBI CCG'CTC
Cuts at: 0 209 3219
Size: 209 3010
BsrDI GCAATG_nn'
Cuts at: 0 402 1830 3074 3219
Size: 402 1428 1244 145
Fragments arranged by size:
1428 1244 402 145
BsrGI T'GTAC_A
Cuts at: 0 1458 3219
Size: 1458 1761
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1635 3219
Size: 1635 1584
BstYI r'GATC_y
Cuts at: 0 269 435 653 737 864 881 1160 1378
Size: 269 166 218 84 127 17 279 218
Cuts at: 1378 3000 3067 3105 3140 3219
Size: 1622 67 38 35 79
Fragments arranged by size:
1622 279 269 218 218 166 127 84
79 67 38 35 17
Bsu36I CC'TnA_GG
Cuts at: 0 2047 3219
Size: 2047 1172
BtgI C'CryG_G
Cuts at: 0 1790 3131 3219
Size: 1790 1341 88
Fragments arranged by size:
1790 1341 88
BtsI GCAGTG_nn'
Cuts at: 0 602 607 2084 3219
Size: 602 5 1477 1135
Fragments arranged by size:
1477 1135 602 5
EaeI y'GGCC_r
Cuts at: 0 1814 3145 3219
Size: 1814 1331 74
Fragments arranged by size:
1814 1331 74
EarI CTCTTCn'nnn_
Cuts at: 0 418 551 764 2957 3219
Size: 418 133 213 2193 262
Fragments arranged by size:
2193 418 262 213 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1927 3219
Size: 1927 1292
EcoICRI GAG'CTC
Cuts at: 0 873 3219
Size: 873 2346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 1171 1181 1195 1369 2002 2480 2706
Size: 127 1044 10 14 174 633 478 226
Cuts at: 2706 3219
Size: 513
Fragments arranged by size:
1044 633 513 478 226 174 127 14
10
EcoO109I rG'GnC_Cy
Cuts at: 0 408 972 3219
Size: 408 564 2247
Fragments arranged by size:
2247 564 408
EcoRI G'AATT_C
Cuts at: 0 178 3219
Size: 178 3041
EcoRV GAT'ATC
Cuts at: 0 2763 3219
Size: 2763 456
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 400 2637 3219
Size: 400 2237 582
Fragments arranged by size:
2237 582 400
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1707 1739 2091 2123 2320 2352 2599 2631
Size: 1707 32 352 32 197 32 247 32
Cuts at: 2631 3074 3106 3219
Size: 443 32 113
Fragments arranged by size:
1707 443 352 247 197 113 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 663 3219
Size: 663 2556
KpnI G_GTAC'C
Cuts at: 0 2588 3219
Size: 2588 631
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2051 2976 3198 3219
Size: 2051 925 222 21
Fragments arranged by size:
2051 925 222 21
MscI TGG'CCA
Cuts at: 0 1816 3219
Size: 1816 1403
MslI CAynn'nnrTG
Cuts at: 0 1247 2656 3019 3021 3219
Size: 1247 1409 363 2 198
Fragments arranged by size:
1409 1247 363 198 2
MspA1I CmG'CkG
Cuts at: 0 105 1501 2544 3219
Size: 105 1396 1043 675
Fragments arranged by size:
1396 1043 675 105
NcoI C'CATG_G
Cuts at: 0 1790 3131 3219
Size: 1790 1341 88
Fragments arranged by size:
1790 1341 88
NheI G'CTAG_C
Cuts at: 0 133 3219
Size: 133 3086
NsiI A_TGCA'T
Cuts at: 0 897 3219
Size: 897 2322
NspI r_CATG'y
Cuts at: 0 1246 1990 2286 3219
Size: 1246 744 296 933
Fragments arranged by size:
1246 933 744 296
PacI TTA_AT'TAA
Cuts at: 0 455 3219
Size: 455 2764
PciI A'CATG_T
Cuts at: 0 2282 3219
Size: 2282 937
PflMI CCAn_nnn'nTGG
Cuts at: 0 1268 3219
Size: 1268 1951
PfoI T'CCnGG_A
Cuts at: 0 2996 3219
Size: 2996 223
PpuMI rG'GwC_Cy
Cuts at: 0 972 3219
Size: 972 2247
PstI C_TGCA'G
Cuts at: 0 1640 3219
Size: 1640 1579
PvuII CAG'CTG
Cuts at: 0 105 1501 2544 3219
Size: 105 1396 1043 675
Fragments arranged by size:
1396 1043 675 105
SacI G_AGCT'C
Cuts at: 0 875 3219
Size: 875 2344
SexAI A'CCwGG_T
Cuts at: 0 2681 3219
Size: 2681 538
SfcI C'TryA_G
Cuts at: 0 1636 1872 2527 3219
Size: 1636 236 655 692
Fragments arranged by size:
1636 692 655 236
SmlI C'TyrA_G
Cuts at: 0 30 1420 3219
Size: 30 1390 1799
Fragments arranged by size:
1799 1390 30
SphI G_CATG'C
Cuts at: 0 1246 3219
Size: 1246 1973
StyI C'CwwG_G
Cuts at: 0 551 968 1790 2111 2819 3131 3219
Size: 551 417 822 321 708 312 88
Fragments arranged by size:
822 708 551 417 321 312 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1324 1906 2689 3219
Size: 1324 582 783 530
Fragments arranged by size:
1324 783 582 530
TatI w'GTAC_w
Cuts at: 0 725 1113 1458 1565 1574 3219
Size: 725 388 345 107 9 1645
Fragments arranged by size:
1645 725 388 345 107 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1386 1418 2357 2389 3219
Size: 1386 32 939 32 830
Fragments arranged by size:
1386 939 830 32 32
XbaI T'CTAG_A
Cuts at: 0 867 3219
Size: 867 2352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2412 3057 3219
Size: 2412 645 162
Fragments arranged by size:
2412 645 162
XmnI GAAnn'nnTTC
Cuts at: 0 959 2668 3219
Size: 959 1709 551
Fragments arranged by size:
1709 959 551
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlfI AlwNI ApoI
BamHI BanI BanII BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmtI BpmI BpuEI BsaI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrGI BstAPI BstYI Bsu36I BtgI BtsI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI Hin4I HincII KpnI MmeI MscI MslI MspA1I
NcoI NheI NsiI NspI PacI PciI PflMI PfoI
PpuMI PstI PvuII SacI SexAI SfcI SmlI SphI
StyI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BbvCI BcgI BglI BmgBI BmrI BplI Bpu10I
BsaAI BsaHI BsiEI BsiWI BsmI BsmBI BspEI BsrFI
BssHII BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EcoNI FseI FspI
FspAI HaeII HindIII HpaI KasI MfeI MluI NaeI
NarI NdeI NgoMIV NotI NruI PasI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI Tth111I XhoI XmaI ZraI