Restriction map for
pF1KSDA0123
(Linear) (Six-base) MAPSORT of: KIAA0123.seq Check: 3668 from: 1 to: 1575
KIAA0123.orf
With 182 enzymes: SgfI *
August 5, 2008 16:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1344 1575
Size: 1344 231
AccI GT'mk_AC
Cuts at: 0 355 1575
Size: 355 1220
AflIII A'CryG_T
Cuts at: 0 908 1575
Size: 908 667
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1303 1335 1575
Size: 1303 32 240
Fragments arranged by size:
1303 240 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1359 1575
Size: 1359 216
ApaI G_GGCC'C
Cuts at: 0 931 1064 1575
Size: 931 133 511
Fragments arranged by size:
931 511 133
ApaLI G'TGCA_C
Cuts at: 0 1395 1575
Size: 1395 180
ApoI r'AATT_y
Cuts at: 0 272 383 1575
Size: 272 111 1192
Fragments arranged by size:
1192 272 111
AvaI C'yCGr_G
Cuts at: 0 42 943 1575
Size: 42 901 632
Fragments arranged by size:
901 632 42
BanI G'GyrC_C
Cuts at: 0 109 1109 1544 1575
Size: 109 1000 435 31
Fragments arranged by size:
1000 435 109 31
BanII G_rGCy'C
Cuts at: 0 43 931 950 1064 1575
Size: 43 888 19 114 511
Fragments arranged by size:
888 511 114 43 19
BbeI G_GCGC'C
Cuts at: 0 1548 1575
Size: 1548 27
BbvCI CC'TCA_GC
Cuts at: 0 67 1575
Size: 67 1508
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 12 46 1575
Size: 12 34 1529
Fragments arranged by size:
1529 34 12
BglI GCCn_nnn'nGGC
Cuts at: 0 499 1575
Size: 499 1076
BglII A'GATC_T
Cuts at: 0 862 1575
Size: 862 713
Bme1580I G_kGCm'C
Cuts at: 0 801 931 1064 1399 1575
Size: 801 130 133 335 176
Fragments arranged by size:
801 335 176 133 130
BmrI ACTGGGnnnn_n'
Cuts at: 0 867 890 1575
Size: 867 23 685
Fragments arranged by size:
867 685 23
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 996 1575
Size: 996 579
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 595 991 1009 1065 1356 1575
Size: 167 428 396 18 56 291 219
Fragments arranged by size:
428 396 291 219 167 56 18
Bpu10I CC'TnA_GC
Cuts at: 0 67 1575
Size: 67 1508
BsaAI yAC'GTr
Cuts at: 0 1505 1575
Size: 1505 70
BsaHI Gr'CG_yC
Cuts at: 0 1545 1575
Size: 1545 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1117 1147 1575
Size: 1117 30 428
Fragments arranged by size:
1117 428 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1006 1575
Size: 1006 569
BsiEI CG_ry'CG
Cuts at: 0 747 1575
Size: 747 828
BsiHKAI G_wGCw'C
Cuts at: 0 779 950 1103 1399 1514 1575
Size: 779 171 153 296 115 61
Fragments arranged by size:
779 296 171 153 115 61
BsmI GAATG_Cn'
Cuts at: 0 714 1575
Size: 714 861
Bsp1286I G_dGCh'C
Cuts at: 0 43 779 801 931 950 1064 1103 1399
Size: 43 736 22 130 19 114 39 296
Cuts at: 1399 1514 1575
Size: 115 61
Fragments arranged by size:
736 296 130 115 114 61 43 39
22 19
BspHI T'CATG_A
Cuts at: 0 627 1304 1575
Size: 627 677 271
Fragments arranged by size:
677 627 271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 596 1344 1505 1575
Size: 596 748 161 70
Fragments arranged by size:
748 596 161 70
BsrFI r'CCGG_y
Cuts at: 0 760 825 1464 1562 1575
Size: 760 65 639 98 13
Fragments arranged by size:
760 639 98 65 13
BsrGI T'GTAC_A
Cuts at: 0 262 1575
Size: 262 1313
BssSI C'ACGA_G
Cuts at: 0 778 1387 1575
Size: 778 609 188
Fragments arranged by size:
778 609 188
BstEII G'GTnAC_C
Cuts at: 0 1483 1575
Size: 1483 92
BstXI CCAn_nnnn'nTGG
Cuts at: 0 880 1575
Size: 880 695
BstYI r'GATC_y
Cuts at: 0 862 1575
Size: 862 713
Bsu36I CC'TnA_GG
Cuts at: 0 724 1575
Size: 724 851
BtgI C'CryG_G
Cuts at: 0 848 1253 1575
Size: 848 405 322
Fragments arranged by size:
848 405 322
BtsI GCAGTG_nn'
Cuts at: 0 1019 1475 1575
Size: 1019 456 100
Fragments arranged by size:
1019 456 100
DrdI GACnn_nn'nnGTC
Cuts at: 0 1527 1575
Size: 1527 48
EaeI y'GGCC_r
Cuts at: 0 758 1361 1472 1575
Size: 758 603 111 103
Fragments arranged by size:
758 603 111 103
EarI CTCTTCn'nnn_
Cuts at: 0 138 1426 1573 1575
Size: 138 1288 147 2
Fragments arranged by size:
1288 147 138 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1049 1262 1575
Size: 1049 213 313
Fragments arranged by size:
1049 313 213
EcoICRI GAG'CTC
Cuts at: 0 948 1575
Size: 948 627
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 595 991 1009 1065 1356 1575
Size: 167 428 396 18 56 291 219
Fragments arranged by size:
428 396 291 219 167 56 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 723 991 1575
Size: 723 268 584
Fragments arranged by size:
723 584 268
EcoO109I rG'GnC_Cy
Cuts at: 0 579 1575
Size: 579 996
EcoRI G'AATT_C
Cuts at: 0 272 1575
Size: 272 1303
HaeII r_GCGC'y
Cuts at: 0 1187 1548 1575
Size: 1187 361 27
Fragments arranged by size:
1187 361 27
KasI G'GCGC_C
Cuts at: 0 1544 1575
Size: 1544 31
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 945 1575
Size: 377 568 630
Fragments arranged by size:
630 568 377
MscI TGG'CCA
Cuts at: 0 1363 1575
Size: 1363 212
MslI CAynn'nnrTG
Cuts at: 0 445 619 1027 1575
Size: 445 174 408 548
Fragments arranged by size:
548 445 408 174
MspA1I CmG'CkG
Cuts at: 0 1290 1575
Size: 1290 285
NaeI GCC'GGC
Cuts at: 0 762 827 1466 1575
Size: 762 65 639 109
Fragments arranged by size:
762 639 109 65
NarI GG'CG_CC
Cuts at: 0 1545 1575
Size: 1545 30
NgoMIV G'CCGG_C
Cuts at: 0 760 825 1464 1575
Size: 760 65 639 111
Fragments arranged by size:
760 639 111 65
NspI r_CATG'y
Cuts at: 0 912 1077 1575
Size: 912 165 498
Fragments arranged by size:
912 498 165
PasI CC'CwG_GG
Cuts at: 0 1479 1575
Size: 1479 96
PciI A'CATG_T
Cuts at: 0 908 1575
Size: 908 667
PfoI T'CCnGG_A
Cuts at: 0 986 1575
Size: 986 589
PpuMI rG'GwC_Cy
Cuts at: 0 579 1575
Size: 579 996
PspOMI G'GGCC_C
Cuts at: 0 927 1060 1575
Size: 927 133 515
Fragments arranged by size:
927 515 133
PstI C_TGCA'G
Cuts at: 0 518 1575
Size: 518 1057
PvuII CAG'CTG
Cuts at: 0 1290 1575
Size: 1290 285
SacI G_AGCT'C
Cuts at: 0 950 1575
Size: 950 625
SapI GCTCTTCn'nnn_
Cuts at: 0 1573 1575
Size: 1573 2
SfcI C'TryA_G
Cuts at: 0 170 514 1575
Size: 170 344 1061
Fragments arranged by size:
1061 344 170
SfoI GGC'GCC
Cuts at: 0 1546 1575
Size: 1546 29
StuI AGG'CCT
Cuts at: 0 1326 1575
Size: 1326 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1471 1575
Size: 1471 104
TatI w'GTAC_w
Cuts at: 0 262 875 1575
Size: 262 613 700
Fragments arranged by size:
700 613 262
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 921 1575
Size: 921 654
Enzymes that do cut:
AarI AccI AflIII AloI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbeI BbvCI BcgI BglI BglII
Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaHI BsaXI
BseRI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI
BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI HaeII KasI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PfoI PpuMI PspOMI
PstI PvuII SacI SapI SfcI SfoI StuI TaqII
TatI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BciVI BclI BfrBI BlpI BmgBI BmtI BpuEI
BsaI BsaBI BsaWI BseYI BsgI BsiWI BsmBI BspEI
BsrBI BsrDI BssHII BstAPI BstBI BstZ17I BtgZI ClaI
CspCI DraI DraIII EagI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StyI SwaI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI