Restriction map for
pF1KA0121
(Linear) (Six-base) MAPSORT of: KIAA0121.seq Check: 5893 from: 1 to: 870
KIAA0121.orf
With 182 enzymes: SgfI *
October 24, 2006 15:05 ..
AfeI AGC'GCT
Cuts at: 0 600 870
Size: 600 270
AflIII A'CryG_T
Cuts at: 0 740 870
Size: 740 130
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 469 501 870
Size: 469 32 369
Fragments arranged by size:
469 369 32
AlwNI CAG_nnn'CTG
Cuts at: 0 102 557 870
Size: 102 455 313
Fragments arranged by size:
455 313 102
AvaI C'yCGr_G
Cuts at: 0 290 459 568 670 870
Size: 290 169 109 102 200
Fragments arranged by size:
290 200 169 109 102
BanI G'GyrC_C
Cuts at: 0 511 678 870
Size: 511 167 192
Fragments arranged by size:
511 192 167
BanII G_rGCy'C
Cuts at: 0 671 706 870
Size: 671 35 164
Fragments arranged by size:
671 164 35
BbeI G_GCGC'C
Cuts at: 0 515 870
Size: 515 355
BbvCI CC'TCA_GC
Cuts at: 0 121 870
Size: 121 749
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 294 328 703 737 870
Size: 294 34 375 34 133
Fragments arranged by size:
375 294 133 34 34
BclI T'GATC_A
Cuts at: 0 491 870
Size: 491 379
BglI GCCn_nnn'nGGC
Cuts at: 0 347 870
Size: 347 523
BmgBI CAC'GTC
Cuts at: 0 219 870
Size: 219 651
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 813 870
Size: 813 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 733 870
Size: 406 327 137
Fragments arranged by size:
406 327 137
Bpu10I CC'TnA_GC
Cuts at: 0 121 870
Size: 121 749
BsaI GGTCTCn'nnnn_
Cuts at: 0 278 870
Size: 278 592
BsaAI yAC'GTr
Cuts at: 0 743 870
Size: 743 127
BsaHI Gr'CG_yC
Cuts at: 0 7 422 512 870
Size: 7 415 90 358
Fragments arranged by size:
415 358 90 7
BsaWI w'CCGG_w
Cuts at: 0 584 870
Size: 584 286
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 126 156 205 235 469 499 835 865
Size: 126 30 49 30 234 30 336 30
Cuts at: 865 870
Size: 5
Fragments arranged by size:
336 234 126 49 30 30 30 30
5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 643 870
Size: 643 227
BseYI C'CCAG_C
Cuts at: 0 157 373 870
Size: 157 216 497
Fragments arranged by size:
497 216 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 870
Size: 328 542
BsmI GAATG_Cn'
Cuts at: 0 98 870
Size: 98 772
BsmBI CGTCTCn'nnnn_
Cuts at: 0 225 870
Size: 225 645
Bsp1286I G_dGCh'C
Cuts at: 0 671 706 870
Size: 671 35 164
Fragments arranged by size:
671 164 35
BsrBI CCG'CTC
Cuts at: 0 297 468 870
Size: 297 171 402
Fragments arranged by size:
402 297 171
BsrFI r'CCGG_y
Cuts at: 0 105 136 436 479 675 870
Size: 105 31 300 43 196 195
Fragments arranged by size:
300 196 195 105 43 31
BsrGI T'GTAC_A
Cuts at: 0 359 870
Size: 359 511
BssHII G'CGCG_C
Cuts at: 0 318 870
Size: 318 552
BstAPI GCAn_nnn'nTGC
Cuts at: 0 102 870
Size: 102 768
BstXI CCAn_nnnn'nTGG
Cuts at: 0 732 870
Size: 732 138
BtgI C'CryG_G
Cuts at: 0 620 707 870
Size: 620 87 163
Fragments arranged by size:
620 163 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 528 870
Size: 528 342
BtsI GCAGTG_nn'
Cuts at: 0 534 870
Size: 534 336
EaeI y'GGCC_r
Cuts at: 0 761 870
Size: 761 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 783 870
Size: 783 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 733 870
Size: 406 327 137
Fragments arranged by size:
406 327 137
EcoNI CCTnn'n_nnAGG
Cuts at: 0 801 870
Size: 801 69
EcoO109I rG'GnC_Cy
Cuts at: 0 195 870
Size: 195 675
HaeII r_GCGC'y
Cuts at: 0 515 602 870
Size: 515 87 268
Fragments arranged by size:
515 268 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 205 237 760 792 870
Size: 7 32 166 32 523 32 78
Fragments arranged by size:
523 166 78 32 32 32 7
KasI G'GCGC_C
Cuts at: 0 511 870
Size: 511 359
MslI CAynn'nnrTG
Cuts at: 0 50 870
Size: 50 820
MspA1I CmG'CkG
Cuts at: 0 554 870
Size: 554 316
NaeI GCC'GGC
Cuts at: 0 438 677 870
Size: 438 239 193
Fragments arranged by size:
438 239 193
NarI GG'CG_CC
Cuts at: 0 512 870
Size: 512 358
NgoMIV G'CCGG_C
Cuts at: 0 436 675 870
Size: 436 239 195
Fragments arranged by size:
436 239 195
NspI r_CATG'y
Cuts at: 0 49 870
Size: 49 821
PmlI CAC'GTG
Cuts at: 0 743 870
Size: 743 127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 469 501 870
Size: 469 32 369
Fragments arranged by size:
469 369 32
PpuMI rG'GwC_Cy
Cuts at: 0 195 870
Size: 195 675
SfoI GGC'GCC
Cuts at: 0 513 870
Size: 513 357
SmaI CCC'GGG
Cuts at: 0 292 461 570 870
Size: 292 169 109 300
Fragments arranged by size:
300 292 169 109
SphI G_CATG'C
Cuts at: 0 49 870
Size: 49 821
SrfI GCCC'GGGC
Cuts at: 0 570 870
Size: 570 300
StyI C'CwwG_G
Cuts at: 0 378 764 870
Size: 378 386 106
Fragments arranged by size:
386 378 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 696 724 870
Size: 696 28 146
Fragments arranged by size:
696 146 28
TatI w'GTAC_w
Cuts at: 0 359 870
Size: 359 511
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 126 158 835 867 870
Size: 126 32 677 32 3
Fragments arranged by size:
677 126 32 32 3
Tth111I GACn'n_nGTC
Cuts at: 0 217 870
Size: 217 653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 381 414 870
Size: 381 33 456
Fragments arranged by size:
456 381 33
XmaI C'CCGG_G
Cuts at: 0 290 459 568 870
Size: 290 169 109 302
Fragments arranged by size:
302 290 169 109
Enzymes that do cut:
AfeI AflIII AloI AlwNI AvaI BanI BanII BbeI
BbvCI BcgI BclI BglI BmgBI BplI BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsmI BsmBI Bsp1286I BsrBI BsrFI BsrGI BssHII BstAPI
BstXI BtgI BtgZI BtsI EaeI EciI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI MslI MspA1I NaeI NarI
NgoMIV NspI PmlI PpiI PpuMI SfoI SmaI SphI
SrfI StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AlfI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BciVI BfrBI BglII
BlpI Bme1580I BmrI BmtI BpuEI BsaBI BsiEI BsiHKAI
BsiWI BspEI BspHI BspMI BsrDI BssSI BstBI BstEII
BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EagI EarI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmlI SnaBI SpeI SspI StuI SwaI
XbaI XhoI XmnI ZraI