Restriction map for
pF1KSDA0120
(Linear) (Six-base) MAPSORT of: KIAA0120.seq Check: 7599 from: 1 to: 597
KIAA0120.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AfeI AGC'GCT
Cuts at: 0 304 597
Size: 304 293
AlwNI CAG_nnn'CTG
Cuts at: 0 87 288 597
Size: 87 201 309
Fragments arranged by size:
309 201 87
AseI AT'TA_AT
Cuts at: 0 197 597
Size: 197 400
AvaI C'yCGr_G
Cuts at: 0 211 413 597
Size: 211 202 184
Fragments arranged by size:
211 202 184
BamHI G'GATC_C
Cuts at: 0 438 597
Size: 438 159
BanI G'GyrC_C
Cuts at: 0 535 597
Size: 535 62
BanII G_rGCy'C
Cuts at: 0 194 428 597
Size: 194 234 169
Fragments arranged by size:
234 194 169
BglII A'GATC_T
Cuts at: 0 72 275 597
Size: 72 203 322
Fragments arranged by size:
322 203 72
BlpI GC'TnA_GC
Cuts at: 0 299 597
Size: 299 298
Bme1580I G_kGCm'C
Cuts at: 0 538 597
Size: 538 59
BmrI ACTGGGnnnn_n'
Cuts at: 0 102 220 597
Size: 102 118 377
Fragments arranged by size:
377 118 102
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 294 597
Size: 294 303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 597
Size: 97 500
Bpu10I CC'TnA_GC
Cuts at: 0 28 597
Size: 28 569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 597
Size: 151 446
BsaBI GATnn'nnATC
Cuts at: 0 71 88 597
Size: 71 17 509
Fragments arranged by size:
509 71 17
BsaHI Gr'CG_yC
Cuts at: 0 550 597
Size: 550 47
BseYI C'CCAG_C
Cuts at: 0 134 597
Size: 134 463
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 394 597
Size: 61 333 203
Fragments arranged by size:
333 203 61
BsiHKAI G_wGCw'C
Cuts at: 0 194 597
Size: 194 403
BsmBI CGTCTCn'nnnn_
Cuts at: 0 557 597
Size: 557 40
Bsp1286I G_dGCh'C
Cuts at: 0 194 428 538 597
Size: 194 234 110 59
Fragments arranged by size:
234 194 110 59
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 24 597
Size: 24 573
BsrFI r'CCGG_y
Cuts at: 0 128 597
Size: 128 469
BstYI r'GATC_y
Cuts at: 0 72 83 236 275 438 590 597
Size: 72 11 153 39 163 152 7
Fragments arranged by size:
163 153 152 72 39 11 7
BtgI C'CryG_G
Cuts at: 0 251 543 597
Size: 251 292 54
Fragments arranged by size:
292 251 54
DraIII CAC_nnn'GTG
Cuts at: 0 110 597
Size: 110 487
EaeI y'GGCC_r
Cuts at: 0 2 597
Size: 2 595
EarI CTCTTCn'nnn_
Cuts at: 0 433 597
Size: 433 164
EcoICRI GAG'CTC
Cuts at: 0 192 597
Size: 192 405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 597
Size: 97 500
EcoO109I rG'GnC_Cy
Cuts at: 0 13 222 597
Size: 13 209 375
Fragments arranged by size:
375 209 13
FseI GG_CCGG'CC
Cuts at: 0 132 597
Size: 132 465
HaeII r_GCGC'y
Cuts at: 0 306 597
Size: 306 291
MscI TGG'CCA
Cuts at: 0 4 597
Size: 4 593
MspA1I CmG'CkG
Cuts at: 0 298 378 498 545 597
Size: 298 80 120 47 52
Fragments arranged by size:
298 120 80 52 47
NaeI GCC'GGC
Cuts at: 0 130 597
Size: 130 467
NcoI C'CATG_G
Cuts at: 0 251 597
Size: 251 346
NdeI CA'TA_TG
Cuts at: 0 21 597
Size: 21 576
NgoMIV G'CCGG_C
Cuts at: 0 128 597
Size: 128 469
PflMI CCAn_nnn'nTGG
Cuts at: 0 339 597
Size: 339 258
PpuMI rG'GwC_Cy
Cuts at: 0 13 597
Size: 13 584
PvuII CAG'CTG
Cuts at: 0 298 498 597
Size: 298 200 99
Fragments arranged by size:
298 200 99
SacI G_AGCT'C
Cuts at: 0 194 597
Size: 194 403
SacII CC_GC'GG
Cuts at: 0 546 597
Size: 546 51
SapI GCTCTTCn'nnn_
Cuts at: 0 433 597
Size: 433 164
SmlI C'TyrA_G
Cuts at: 0 166 597
Size: 166 431
StuI AGG'CCT
Cuts at: 0 244 597
Size: 244 353
StyI C'CwwG_G
Cuts at: 0 251 464 597
Size: 251 213 133
Fragments arranged by size:
251 213 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 121 385 597
Size: 121 264 212
Fragments arranged by size:
264 212 121
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 575 597
Size: 575 22
Enzymes that do cut:
AfeI AlwNI AseI AvaI BamHI BanI BanII BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaBI
BsaHI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrFI
BstYI BtgI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I
FseI HaeII MscI MspA1I NaeI NcoI NdeI NgoMIV
PflMI PpuMI PvuII SacI SacII SapI SmlI StuI
StyI TaqII TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BmgBI BmtI BsaI BsaAI
BsaWI BsaXI BseRI BsiEI BsiWI BsmI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DrdI EagI EciI EcoNI EcoRI EcoRV FalI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MslI NarI NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI