Restriction map for
pF1KA0113
(Linear) (Six-base) MAPSORT of: KIAA0113.seq Check: 5001 from: 1 to: 1908
KIAA0113.orf
With 182 enzymes: SgfI *
October 24, 2006 15:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 488 1032 1383 1908
Size: 488 544 351 525
Fragments arranged by size:
544 525 488 351
AccI GT'mk_AC
Cuts at: 0 1797 1908
Size: 1797 111
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 685 1489 1908
Size: 124 561 804 419
Fragments arranged by size:
804 561 419 124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 487 952 1908
Size: 487 465 956
Fragments arranged by size:
956 487 465
AlwNI CAG_nnn'CTG
Cuts at: 0 307 489 630 832 1023 1362 1908
Size: 307 182 141 202 191 339 546
Fragments arranged by size:
546 339 307 202 191 182 141
ApaI G_GGCC'C
Cuts at: 0 445 1344 1908
Size: 445 899 564
Fragments arranged by size:
899 564 445
ApaLI G'TGCA_C
Cuts at: 0 592 1908
Size: 592 1316
ApoI r'AATT_y
Cuts at: 0 93 370 399 729 1908
Size: 93 277 29 330 1179
Fragments arranged by size:
1179 330 277 93 29
BanI G'GyrC_C
Cuts at: 0 358 1674 1908
Size: 358 1316 234
Fragments arranged by size:
1316 358 234
BanII G_rGCy'C
Cuts at: 0 244 266 445 740 1210 1327 1344 1616
Size: 244 22 179 295 470 117 17 272
Cuts at: 1616 1834 1908
Size: 218 74
Fragments arranged by size:
470 295 272 244 218 179 117 74
22 17
BglII A'GATC_T
Cuts at: 0 1400 1908
Size: 1400 508
BlpI GC'TnA_GC
Cuts at: 0 260 1204 1908
Size: 260 944 704
Fragments arranged by size:
944 704 260
Bme1580I G_kGCm'C
Cuts at: 0 50 445 596 1344 1908
Size: 50 395 151 748 564
Fragments arranged by size:
748 564 395 151 50
BmrI ACTGGGnnnn_n'
Cuts at: 0 311 1908
Size: 311 1597
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1576 1908
Size: 1576 332
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 586 937 1075 1288 1908
Size: 511 75 351 138 213 620
Fragments arranged by size:
620 511 351 213 138 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 1908
Size: 724 1184
BsaI GGTCTCn'nnnn_
Cuts at: 0 487 1126 1908
Size: 487 639 782
Fragments arranged by size:
782 639 487
BsaHI Gr'CG_yC
Cuts at: 0 1794 1908
Size: 1794 114
BsaWI w'CCGG_w
Cuts at: 0 20 971 1908
Size: 20 951 937
Fragments arranged by size:
951 937 20
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 279 309 1908
Size: 279 30 1599
Fragments arranged by size:
1599 279 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 354 406 532 1090 1144 1423 1468
Size: 205 149 52 126 558 54 279 45
Cuts at: 1468 1555 1908
Size: 87 353
Fragments arranged by size:
558 353 279 205 149 126 87 54
52 45
BseYI C'CCAG_C
Cuts at: 0 438 1517 1908
Size: 438 1079 391
Fragments arranged by size:
1079 438 391
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 1908
Size: 460 1448
BsiHKAI G_wGCw'C
Cuts at: 0 266 596 740 1742 1834 1908
Size: 266 330 144 1002 92 74
Fragments arranged by size:
1002 330 266 144 92 74
BsmI GAATG_Cn'
Cuts at: 0 1528 1908
Size: 1528 380
Bsp1286I G_dGCh'C
Cuts at: 0 50 244 266 445 596 740 1210 1327
Size: 50 194 22 179 151 144 470 117
Cuts at: 1327 1344 1616 1742 1834 1908
Size: 17 272 126 92 74
Fragments arranged by size:
470 272 194 179 151 144 126 117
92 74 50 22 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 488 850 1032 1199 1383 1908
Size: 488 362 182 167 184 525
Fragments arranged by size:
525 488 362 184 182 167
BsrBI CCG'CTC
Cuts at: 0 1065 1908
Size: 1065 843
BsrDI GCAATG_nn'
Cuts at: 0 765 1908
Size: 765 1143
BstBI TT'CG_AA
Cuts at: 0 1815 1908
Size: 1815 93
BstEII G'GTnAC_C
Cuts at: 0 1500 1908
Size: 1500 408
BstXI CCAn_nnnn'nTGG
Cuts at: 0 432 1734 1908
Size: 432 1302 174
Fragments arranged by size:
1302 432 174
BstYI r'GATC_y
Cuts at: 0 23 1244 1400 1706 1908
Size: 23 1221 156 306 202
Fragments arranged by size:
1221 306 202 156 23
Bsu36I CC'TnA_GG
Cuts at: 0 51 389 1348 1590 1908
Size: 51 338 959 242 318
Fragments arranged by size:
959 338 318 242 51
BtgI C'CryG_G
Cuts at: 0 528 1670 1683 1727 1733 1908
Size: 528 1142 13 44 6 175
Fragments arranged by size:
1142 528 175 44 13 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 445 1908
Size: 445 1463
BtsI GCAGTG_nn'
Cuts at: 0 833 936 961 1437 1908
Size: 833 103 25 476 471
Fragments arranged by size:
833 476 471 103 25
DrdI GACnn_nn'nnGTC
Cuts at: 0 283 1908
Size: 283 1625
EaeI y'GGCC_r
Cuts at: 0 531 787 1106 1730 1908
Size: 531 256 319 624 178
Fragments arranged by size:
624 531 319 256 178
EarI CTCTTCn'nnn_
Cuts at: 0 143 544 1457 1567 1774 1908
Size: 143 401 913 110 207 134
Fragments arranged by size:
913 401 207 143 134 110
EcoICRI GAG'CTC
Cuts at: 0 264 738 1832 1908
Size: 264 474 1094 76
Fragments arranged by size:
1094 474 264 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 511 586 685 937 1075 1288 1489
Size: 124 387 75 99 252 138 213 201
Cuts at: 1489 1908
Size: 419
Fragments arranged by size:
419 387 252 213 201 138 124 99
75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 85 1908
Size: 85 1823
EcoO109I rG'GnC_Cy
Cuts at: 0 442 684 867 1263 1340 1341 1637 1900
Size: 442 242 183 396 77 1 296 263
Cuts at: 1900 1908
Size: 8
Fragments arranged by size:
442 396 296 263 242 183 77 8
1
EcoRI G'AATT_C
Cuts at: 0 93 399 1908
Size: 93 306 1509
Fragments arranged by size:
1509 306 93
HaeII r_GCGC'y
Cuts at: 0 81 439 489 1908
Size: 81 358 50 1419
Fragments arranged by size:
1419 358 81 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 763 795 1684 1716 1833 1865 1908
Size: 763 32 889 32 117 32 43
Fragments arranged by size:
889 763 117 43 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 1908
Size: 656 1252
MscI TGG'CCA
Cuts at: 0 1108 1732 1908
Size: 1108 624 176
Fragments arranged by size:
1108 624 176
MslI CAynn'nnrTG
Cuts at: 0 425 599 1732 1908
Size: 425 174 1133 176
Fragments arranged by size:
1133 425 176 174
MspA1I CmG'CkG
Cuts at: 0 696 1149 1203 1251 1908
Size: 696 453 54 48 657
Fragments arranged by size:
696 657 453 54 48
NcoI C'CATG_G
Cuts at: 0 1670 1683 1727 1733 1908
Size: 1670 13 44 6 175
Fragments arranged by size:
1670 175 44 13 6
NspI r_CATG'y
Cuts at: 0 314 1908
Size: 314 1594
PasI CC'CwG_GG
Cuts at: 0 34 1908
Size: 34 1874
PflMI CCAn_nnn'nTGG
Cuts at: 0 355 1684 1842 1908
Size: 355 1329 158 66
Fragments arranged by size:
1329 355 158 66
PpuMI rG'GwC_Cy
Cuts at: 0 867 1908
Size: 867 1041
PspOMI G'GGCC_C
Cuts at: 0 441 1340 1908
Size: 441 899 568
Fragments arranged by size:
899 568 441
PstI C_TGCA'G
Cuts at: 0 485 626 1196 1493 1908
Size: 485 141 570 297 415
Fragments arranged by size:
570 485 415 297 141
PvuII CAG'CTG
Cuts at: 0 1149 1203 1908
Size: 1149 54 705
Fragments arranged by size:
1149 705 54
SacI G_AGCT'C
Cuts at: 0 266 740 1834 1908
Size: 266 474 1094 74
Fragments arranged by size:
1094 474 266 74
SapI GCTCTTCn'nnn_
Cuts at: 0 544 1457 1908
Size: 544 913 451
Fragments arranged by size:
913 544 451
SbfI CC_TGCA'GG
Cuts at: 0 1196 1908
Size: 1196 712
SfcI C'TryA_G
Cuts at: 0 481 622 1192 1489 1865 1908
Size: 481 141 570 297 376 43
Fragments arranged by size:
570 481 376 297 141 43
SmlI C'TyrA_G
Cuts at: 0 739 1908
Size: 739 1169
SphI G_CATG'C
Cuts at: 0 314 1908
Size: 314 1594
StuI AGG'CCT
Cuts at: 0 1588 1863 1908
Size: 1588 275 45
Fragments arranged by size:
1588 275 45
StyI C'CwwG_G
Cuts at: 0 236 587 884 1163 1670 1683 1727 1733
Size: 236 351 297 279 507 13 44 6
Cuts at: 1733 1908
Size: 175
Fragments arranged by size:
507 351 297 279 236 175 44 13
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 559 1150 1634 1908
Size: 559 591 484 274
Fragments arranged by size:
591 559 484 274
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 279 311 1268 1300 1908
Size: 279 32 957 32 608
Fragments arranged by size:
957 608 279 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1122 1668 1908
Size: 1122 546 240
Fragments arranged by size:
1122 546 240
XmnI GAAnn'nnTTC
Cuts at: 0 1402 1908
Size: 1402 506
Enzymes that do cut:
AarI AccI AcuI AlfI AlwNI ApaI ApaLI ApoI
BanI BanII BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
MmeI MscI MslI MspA1I NcoI NspI PasI PflMI
PpuMI PspOMI PstI PvuII SacI SapI SbfI SfcI
SmlI SphI StuI StyI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BmgBI BmtI Bpu10I BsaAI BsaBI BsiEI BsiWI
BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI BstAPI
BstZ17I ClaI CspCI DraI DraIII EagI EciI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TatI Tth111I
XbaI XhoI XmaI ZraI