Restriction map for
pF1KSDA0111
(Linear) (Six-base) MAPSORT of: KIAA0111.seq Check: 2588 from: 1 to: 1233
KIAA0111.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AccI GT'mk_AC
Cuts at: 0 611 1233
Size: 611 622
Acc65I G'GTAC_C
Cuts at: 0 617 1233
Size: 617 616
AclI AA'CG_TT
Cuts at: 0 1217 1233
Size: 1217 16
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 1233
Size: 258 975
AflIII A'CryG_T
Cuts at: 0 532 1233
Size: 532 701
AleI CACnn'nnGTG
Cuts at: 0 637 1233
Size: 637 596
ApoI r'AATT_y
Cuts at: 0 79 790 1233
Size: 79 711 443
Fragments arranged by size:
711 443 79
AvaI C'yCGr_G
Cuts at: 0 27 1233
Size: 27 1206
BanI G'GyrC_C
Cuts at: 0 427 617 976 1233
Size: 427 190 359 257
Fragments arranged by size:
427 359 257 190
BanII G_rGCy'C
Cuts at: 0 34 1233
Size: 34 1199
BbeI G_GCGC'C
Cuts at: 0 980 1233
Size: 980 253
BbsI GAAGACnn'nnnn_
Cuts at: 0 68 1233
Size: 68 1165
BciVI GTATCCnnnnn_n'
Cuts at: 0 921 1233
Size: 921 312
BfrBI ATG'CAT
Cuts at: 0 921 1233
Size: 921 312
BglI GCCn_nnn'nGGC
Cuts at: 0 19 1233
Size: 19 1214
Bme1580I G_kGCm'C
Cuts at: 0 487 1233
Size: 487 746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 473 611 697 1233
Size: 274 199 138 86 536
Fragments arranged by size:
536 274 199 138 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 1233
Size: 305 928
BsaI GGTCTCn'nnnn_
Cuts at: 0 79 1233
Size: 79 1154
BsaAI yAC'GTr
Cuts at: 0 535 1233
Size: 535 698
BsaHI Gr'CG_yC
Cuts at: 0 977 1233
Size: 977 256
BsaWI w'CCGG_w
Cuts at: 0 968 1233
Size: 968 265
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 955 985 1089 1119 1233
Size: 955 30 104 30 114
Fragments arranged by size:
955 114 104 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 1233
Size: 109 1124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 1233
Size: 379 854
BsiEI CG_ry'CG
Cuts at: 0 973 1116 1233
Size: 973 143 117
Fragments arranged by size:
973 143 117
Bsp1286I G_dGCh'C
Cuts at: 0 34 487 1233
Size: 34 453 746
Fragments arranged by size:
746 453 34
BspHI T'CATG_A
Cuts at: 0 695 956 1233
Size: 695 261 277
Fragments arranged by size:
695 277 261
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 152 629 1233
Size: 152 477 604
Fragments arranged by size:
604 477 152
BsrBI CCG'CTC
Cuts at: 0 945 1233
Size: 945 288
BsrGI T'GTAC_A
Cuts at: 0 1079 1233
Size: 1079 154
BssSI C'ACGA_G
Cuts at: 0 667 1233
Size: 667 566
BstXI CCAn_nnnn'nTGG
Cuts at: 0 639 1020 1233
Size: 639 381 213
Fragments arranged by size:
639 381 213
BstYI r'GATC_y
Cuts at: 0 362 1233
Size: 362 871
BstZ17I GTA'TAC
Cuts at: 0 612 1233
Size: 612 621
Bsu36I CC'TnA_GG
Cuts at: 0 526 1031 1233
Size: 526 505 202
Fragments arranged by size:
526 505 202
BtgI C'CryG_G
Cuts at: 0 8 125 1233
Size: 8 117 1108
Fragments arranged by size:
1108 117 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 219 1233
Size: 219 1014
BtsI GCAGTG_nn'
Cuts at: 0 776 1233
Size: 776 457
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1179 1214 1233
Size: 1179 35 19
Fragments arranged by size:
1179 35 19
DraIII CAC_nnn'GTG
Cuts at: 0 803 1233
Size: 803 430
EaeI y'GGCC_r
Cuts at: 0 11 1113 1127 1233
Size: 11 1102 14 106
Fragments arranged by size:
1102 106 14 11
EagI C'GGCC_G
Cuts at: 0 1113 1233
Size: 1113 120
EarI CTCTTCn'nnn_
Cuts at: 0 776 1233
Size: 776 457
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 274 473 611 697 1233
Size: 258 16 199 138 86 536
Fragments arranged by size:
536 258 199 138 86 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 136 1233
Size: 136 1097
EcoO109I rG'GnC_Cy
Cuts at: 0 141 1233
Size: 141 1092
EcoRI G'AATT_C
Cuts at: 0 79 1233
Size: 79 1154
EcoRV GAT'ATC
Cuts at: 0 1173 1233
Size: 1173 60
HaeII r_GCGC'y
Cuts at: 0 980 1233
Size: 980 253
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 218 250 321 353 816 848 934 955
Size: 218 32 71 32 463 32 86 21
Cuts at: 955 966 987 1089 1121 1233
Size: 11 21 102 32 112
Fragments arranged by size:
463 218 112 102 86 71 32 32
32 32 21 21 11
HindIII A'AGCT_T
Cuts at: 0 323 1233
Size: 323 910
KasI G'GCGC_C
Cuts at: 0 976 1233
Size: 976 257
KpnI G_GTAC'C
Cuts at: 0 621 1233
Size: 621 612
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1001 1233
Size: 1001 232
MscI TGG'CCA
Cuts at: 0 1129 1233
Size: 1129 104
MslI CAynn'nnrTG
Cuts at: 0 637 718 1233
Size: 637 81 515
Fragments arranged by size:
637 515 81
NarI GG'CG_CC
Cuts at: 0 977 1233
Size: 977 256
NcoI C'CATG_G
Cuts at: 0 125 1233
Size: 125 1108
NsiI A_TGCA'T
Cuts at: 0 923 1233
Size: 923 310
NspI r_CATG'y
Cuts at: 0 475 933 1233
Size: 475 458 300
Fragments arranged by size:
475 458 300
PflMI CCAn_nnn'nTGG
Cuts at: 0 297 1233
Size: 297 936
PmlI CAC'GTG
Cuts at: 0 535 1233
Size: 535 698
PpuMI rG'GwC_Cy
Cuts at: 0 141 1233
Size: 141 1092
ScaI AGT'ACT
Cuts at: 0 1183 1233
Size: 1183 50
SfcI C'TryA_G
Cuts at: 0 998 1233
Size: 998 235
SfoI GGC'GCC
Cuts at: 0 978 1233
Size: 978 255
SmlI C'TyrA_G
Cuts at: 0 320 1233
Size: 320 913
StyI C'CwwG_G
Cuts at: 0 125 1233
Size: 125 1108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 715 1233
Size: 715 518
TatI w'GTAC_w
Cuts at: 0 1079 1181 1233
Size: 1079 102 52
Fragments arranged by size:
1079 102 52
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 15 96 348 418 864 1233
Size: 15 81 252 70 446 369
Fragments arranged by size:
446 369 252 81 70 15
XmnI GAAnn'nnTTC
Cuts at: 0 964 1233
Size: 964 269
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI ApoI AvaI
BanI BanII BbeI BbsI BciVI BfrBI BglI Bme1580I
BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BsgI BsiEI Bsp1286I BspHI BspMI BsrBI BsrGI BssSI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraIII EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI
EcoRV HaeII Hin4I HindIII KasI KpnI MmeI MscI
MslI NarI NcoI NsiI NspI PflMI PmlI PpuMI
ScaI SfcI SfoI SmlI StyI TaqII TatI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BclI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BsaBI BseYI BsiHKAI BsiWI BsmI
BsmBI BspEI BsrDI BsrFI BssHII BstAPI BstBI BstEII
ClaI DraI DrdI EciI EcoICRI FalI FseI FspI
FspAI HincII HpaI MfeI MluI MspA1I NaeI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PfoI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XhoI XmaI ZraI