Restriction map for
pF1KA0110
(Linear) (Six-base) MAPSORT of: KIAA0110.seq Check: 6666 from: 1 to: 822
KIAA0110.orf
With 182 enzymes: SgfI *
November 13, 2006 11:20 ..
Acc65I G'GTAC_C
Cuts at: 0 165 822
Size: 165 657
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 592 822
Size: 346 246 230
Fragments arranged by size:
346 246 230
AflII C'TTAA_G
Cuts at: 0 280 822
Size: 280 542
AleI CACnn'nnGTG
Cuts at: 0 352 822
Size: 352 470
AlwNI CAG_nnn'CTG
Cuts at: 0 43 540 822
Size: 43 497 282
Fragments arranged by size:
497 282 43
AvaI C'yCGr_G
Cuts at: 0 338 822
Size: 338 484
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 422 455 822
Size: 422 33 367
Fragments arranged by size:
422 367 33
BanI G'GyrC_C
Cuts at: 0 6 165 628 792 822
Size: 6 159 463 164 30
Fragments arranged by size:
463 164 159 30 6
BbeI G_GCGC'C
Cuts at: 0 10 822
Size: 10 812
BbsI GAAGACnn'nnnn_
Cuts at: 0 779 822
Size: 779 43
BbvCI CC'TCA_GC
Cuts at: 0 30 403 822
Size: 30 373 419
Fragments arranged by size:
419 373 30
BglI GCCn_nnn'nGGC
Cuts at: 0 200 822
Size: 200 622
Bme1580I G_kGCm'C
Cuts at: 0 631 704 822
Size: 631 73 118
Fragments arranged by size:
631 118 73
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 126 822
Size: 126 696
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 433 822
Size: 412 21 389
Fragments arranged by size:
412 389 21
Bpu10I CC'TnA_GC
Cuts at: 0 30 403 475 541 822
Size: 30 373 72 66 281
Fragments arranged by size:
373 281 72 66 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 822
Size: 121 701
BsaHI Gr'CG_yC
Cuts at: 0 7 822
Size: 7 815
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 10 40 822
Size: 10 30 782
Fragments arranged by size:
782 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 331 605 822
Size: 331 274 217
Fragments arranged by size:
331 274 217
BseYI C'CCAG_C
Cuts at: 0 703 822
Size: 703 119
BsiHKAI G_wGCw'C
Cuts at: 0 547 822
Size: 547 275
BsmBI CGTCTCn'nnnn_
Cuts at: 0 571 822
Size: 571 251
Bsp1286I G_dGCh'C
Cuts at: 0 547 631 704 822
Size: 547 84 73 118
Fragments arranged by size:
547 118 84 73
BsrDI GCAATG_nn'
Cuts at: 0 474 822
Size: 474 348
BstAPI GCAn_nnn'nTGC
Cuts at: 0 368 822
Size: 368 454
BstXI CCAn_nnnn'nTGG
Cuts at: 0 585 822
Size: 585 237
BtgI C'CryG_G
Cuts at: 0 761 822
Size: 761 61
DraI TTT'AAA
Cuts at: 0 807 822
Size: 807 15
EarI CTCTTCn'nnn_
Cuts at: 0 574 822
Size: 574 248
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 31 822
Size: 31 791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 412 433 592 822
Size: 346 66 21 159 230
Fragments arranged by size:
346 230 159 66 21
HaeII r_GCGC'y
Cuts at: 0 10 822
Size: 10 812
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 671 703 737 769 822
Size: 10 32 629 32 34 32 53
Fragments arranged by size:
629 53 34 32 32 32 10
KasI G'GCGC_C
Cuts at: 0 6 822
Size: 6 816
KpnI G_GTAC'C
Cuts at: 0 169 822
Size: 169 653
MslI CAynn'nnrTG
Cuts at: 0 352 583 822
Size: 352 231 239
Fragments arranged by size:
352 239 231
NarI GG'CG_CC
Cuts at: 0 7 822
Size: 7 815
PflMI CCAn_nnn'nTGG
Cuts at: 0 767 822
Size: 767 55
SfcI C'TryA_G
Cuts at: 0 522 822
Size: 522 300
SfoI GGC'GCC
Cuts at: 0 8 822
Size: 8 814
SmlI C'TyrA_G
Cuts at: 0 100 280 822
Size: 100 180 542
Fragments arranged by size:
542 180 100
SpeI A'CTAG_T
Cuts at: 0 435 822
Size: 435 387
StuI AGG'CCT
Cuts at: 0 142 822
Size: 142 680
StyI C'CwwG_G
Cuts at: 0 459 482 822
Size: 459 23 340
Fragments arranged by size:
459 340 23
Tth111I GACn'n_nGTC
Cuts at: 0 732 822
Size: 732 90
XmnI GAAnn'nnTTC
Cuts at: 0 300 822
Size: 300 522
Enzymes that do cut:
Acc65I AcuI AflII AleI AlwNI AvaI BaeI BanI
BbeI BbsI BbvCI BglI Bme1580I BplI BpmI Bpu10I
BpuEI BsaHI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I
BsrDI BstAPI BstXI BtgI DraI EarI EciI Eco57MI
HaeII Hin4I KasI KpnI MslI NarI PflMI SfcI
SfoI SmlI SpeI StuI StyI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflIII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BanII BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BsaI BsaAI BsaBI BsaWI
BsgI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI EaeI
EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI MfeI MluI MmeI
MscI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SphI SrfI SspI SwaI TaqII TatI TstI
XbaI XcmI XhoI XmaI ZraI