Restriction map for
pF1KA0092
(Linear) (Six-base) MAPSORT of: KIAA0092.seq Check: 5010 from: 1 to: 1422
KIAA0092.orf
With 182 enzymes: SgfI *
October 24, 2006 15:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1339 1422
Size: 1339 83
AflII C'TTAA_G
Cuts at: 0 217 1422
Size: 217 1205
AflIII A'CryG_T
Cuts at: 0 540 1422
Size: 540 882
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 629 1422
Size: 629 793
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 68 100 1422
Size: 68 32 1322
Fragments arranged by size:
1322 68 32
ApoI r'AATT_y
Cuts at: 0 348 1030 1386 1422
Size: 348 682 356 36
Fragments arranged by size:
682 356 348 36
AseI AT'TA_AT
Cuts at: 0 989 1422
Size: 989 433
BclI T'GATC_A
Cuts at: 0 531 1050 1422
Size: 531 519 372
Fragments arranged by size:
531 519 372
BlpI GC'TnA_GC
Cuts at: 0 56 1422
Size: 56 1366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1216 1352 1422
Size: 1216 136 70
Fragments arranged by size:
1216 136 70
Bpu10I CC'TnA_GC
Cuts at: 0 686 1422
Size: 686 736
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 1422
Size: 274 1148
BsaI GGTCTCn'nnnn_
Cuts at: 0 1320 1422
Size: 1320 102
BsaHI Gr'CG_yC
Cuts at: 0 10 1422
Size: 10 1412
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 966 1009 1422
Size: 966 43 413
Fragments arranged by size:
966 413 43
BseYI C'CCAG_C
Cuts at: 0 1190 1422
Size: 1190 232
BsmI GAATG_Cn'
Cuts at: 0 90 1422
Size: 90 1332
BsrDI GCAATG_nn'
Cuts at: 0 894 1422
Size: 894 528
BssSI C'ACGA_G
Cuts at: 0 935 1422
Size: 935 487
BstBI TT'CG_AA
Cuts at: 0 1176 1422
Size: 1176 246
BstYI r'GATC_y
Cuts at: 0 567 1422
Size: 567 855
BtgI C'CryG_G
Cuts at: 0 827 1422
Size: 827 595
DraIII CAC_nnn'GTG
Cuts at: 0 940 1422
Size: 940 482
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1216 1339 1352 1422
Size: 1216 123 13 70
Fragments arranged by size:
1216 123 70 13
EcoO109I rG'GnC_Cy
Cuts at: 0 609 1422
Size: 609 813
EcoRI G'AATT_C
Cuts at: 0 348 1386 1422
Size: 348 1038 36
Fragments arranged by size:
1038 348 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 469 501 985 1017 1035 1067 1335 1367
Size: 469 32 484 32 18 32 268 32
Cuts at: 1367 1422
Size: 55
Fragments arranged by size:
484 469 268 55 32 32 32 32
18
HindIII A'AGCT_T
Cuts at: 0 878 1422
Size: 878 544
MfeI C'AATT_G
Cuts at: 0 302 436 1422
Size: 302 134 986
Fragments arranged by size:
986 302 134
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 1004 1091 1422
Size: 617 387 87 331
Fragments arranged by size:
617 387 331 87
MspA1I CmG'CkG
Cuts at: 0 694 1194 1422
Size: 694 500 228
Fragments arranged by size:
694 500 228
NdeI CA'TA_TG
Cuts at: 0 105 1422
Size: 105 1317
NspI r_CATG'y
Cuts at: 0 450 469 544 681 1374 1422
Size: 450 19 75 137 693 48
Fragments arranged by size:
693 450 137 75 48 19
PciI A'CATG_T
Cuts at: 0 540 1422
Size: 540 882
PflMI CCAn_nnn'nTGG
Cuts at: 0 828 1422
Size: 828 594
PfoI T'CCnGG_A
Cuts at: 0 1076 1422
Size: 1076 346
PshAI GACnn'nnGTC
Cuts at: 0 487 1422
Size: 487 935
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 824 856 1422
Size: 824 32 566
Fragments arranged by size:
824 566 32
PstI C_TGCA'G
Cuts at: 0 408 1422
Size: 408 1014
PvuII CAG'CTG
Cuts at: 0 694 1194 1422
Size: 694 500 228
Fragments arranged by size:
694 500 228
ScaI AGT'ACT
Cuts at: 0 320 1422
Size: 320 1102
SfcI C'TryA_G
Cuts at: 0 404 1422
Size: 404 1018
SmlI C'TyrA_G
Cuts at: 0 217 253 1422
Size: 217 36 1169
Fragments arranged by size:
1169 217 36
SphI G_CATG'C
Cuts at: 0 469 681 1422
Size: 469 212 741
Fragments arranged by size:
741 469 212
TatI w'GTAC_w
Cuts at: 0 318 1422
Size: 318 1104
XbaI T'CTAG_A
Cuts at: 0 292 711 1422
Size: 292 419 711
Fragments arranged by size:
711 419 292
Enzymes that do cut:
AcuI AflII AflIII AlfI AloI ApoI AseI BclI
BlpI BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI
BsmI BsrDI BssSI BstBI BstYI BtgI DraIII Eco57MI
EcoO109I EcoRI Hin4I HindIII MfeI MmeI MspA1I NdeI
NspI PciI PflMI PfoI PshAI PsrI PstI PvuII
ScaI SfcI SmlI SphI TatI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI
BplI BsaAI BsaBI BsaWI BsaXI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MluI MscI MslI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PasI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI StyI SwaI TaqII TstI
Tth111I XcmI XhoI XmaI XmnI ZraI