Restriction map for
pF1KSDA0091
(Linear) (Six-base) MAPSORT of: KIAA0091.seq Check: 8530 from: 1 to: 3156
KIAA0091.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 751 1252 3156
Size: 751 501 1904
Fragments arranged by size:
1904 751 501
AccI GT'mk_AC
Cuts at: 0 245 3156
Size: 245 2911
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 2132 2151 3156
Size: 250 1882 19 1005
Fragments arranged by size:
1882 1005 250 19
AflIII A'CryG_T
Cuts at: 0 905 1370 1487 2047 2351 3156
Size: 905 465 117 560 304 805
Fragments arranged by size:
905 805 560 465 304 117
AhdI GACnn_n'nnGTC
Cuts at: 0 1478 3156
Size: 1478 1678
AleI CACnn'nnGTG
Cuts at: 0 754 3156
Size: 754 2402
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 568 3156
Size: 568 2588
AlwNI CAG_nnn'CTG
Cuts at: 0 588 1267 1335 2001 2174 3156
Size: 588 679 68 666 173 982
Fragments arranged by size:
982 679 666 588 173 68
ApaI G_GGCC'C
Cuts at: 0 1227 2040 3156
Size: 1227 813 1116
Fragments arranged by size:
1227 1116 813
ApoI r'AATT_y
Cuts at: 0 136 207 3156
Size: 136 71 2949
Fragments arranged by size:
2949 136 71
AvaI C'yCGr_G
Cuts at: 0 1360 2032 3156
Size: 1360 672 1124
Fragments arranged by size:
1360 1124 672
BanI G'GyrC_C
Cuts at: 0 1217 2823 2941 3156
Size: 1217 1606 118 215
Fragments arranged by size:
1606 1217 215 118
BanII G_rGCy'C
Cuts at: 0 511 1227 1456 2040 3156
Size: 511 716 229 584 1116
Fragments arranged by size:
1116 716 584 511 229
BbeI G_GCGC'C
Cuts at: 0 2827 2945 3156
Size: 2827 118 211
Fragments arranged by size:
2827 211 118
BbvCI CC'TCA_GC
Cuts at: 0 916 2504 3156
Size: 916 1588 652
Fragments arranged by size:
1588 916 652
BciVI GTATCCnnnnn_n'
Cuts at: 0 178 2238 3156
Size: 178 2060 918
Fragments arranged by size:
2060 918 178
BclI T'GATC_A
Cuts at: 0 1050 1751 1962 3156
Size: 1050 701 211 1194
Fragments arranged by size:
1194 1050 701 211
BglI GCCn_nnn'nGGC
Cuts at: 0 1599 3156
Size: 1599 1557
BglII A'GATC_T
Cuts at: 0 1467 3156
Size: 1467 1689
BlpI GC'TnA_GC
Cuts at: 0 661 3156
Size: 661 2495
Bme1580I G_kGCm'C
Cuts at: 0 228 1227 2040 3156
Size: 228 999 813 1116
Fragments arranged by size:
1116 999 813 228
BmgBI CAC'GTC
Cuts at: 0 478 906 2139 3156
Size: 478 428 1233 1017
Fragments arranged by size:
1233 1017 478 428
BmrI ACTGGGnnnn_n'
Cuts at: 0 665 2660 2822 3156
Size: 665 1995 162 334
Fragments arranged by size:
1995 665 334 162
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 514 3156
Size: 514 2642
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 785 1235 2584 2672 2755 2951 2978 3156
Size: 785 450 1349 88 83 196 27 178
Fragments arranged by size:
1349 785 450 196 178 88 83 27
Bpu10I CC'TnA_GC
Cuts at: 0 916 1736 2504 3156
Size: 916 820 768 652
Fragments arranged by size:
916 820 768 652
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 2894 3156
Size: 391 2503 262
Fragments arranged by size:
2503 391 262
BsaI GGTCTCn'nnnn_
Cuts at: 0 2744 3156
Size: 2744 412
BsaAI yAC'GTr
Cuts at: 0 2048 3156
Size: 2048 1108
BsaHI Gr'CG_yC
Cuts at: 0 872 2399 2824 2942 3156
Size: 872 1527 425 118 214
Fragments arranged by size:
1527 872 425 214 118
BsaWI w'CCGG_w
Cuts at: 0 2129 2248 3156
Size: 2129 119 908
Fragments arranged by size:
2129 908 119
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3017 3047 3156
Size: 3017 30 109
Fragments arranged by size:
3017 109 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1644 2110 2113 2267 2587 3156
Size: 1644 466 3 154 320 569
Fragments arranged by size:
1644 569 466 320 154 3
BseYI C'CCAG_C
Cuts at: 0 1456 1505 1767 2265 3156
Size: 1456 49 262 498 891
Fragments arranged by size:
1456 891 498 262 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1814 2391 3156
Size: 1814 577 765
Fragments arranged by size:
1814 765 577
BsiEI CG_ry'CG
Cuts at: 0 1155 2899 3156
Size: 1155 1744 257
Fragments arranged by size:
1744 1155 257
BsiHKAI G_wGCw'C
Cuts at: 0 605 1288 3156
Size: 605 683 1868
Fragments arranged by size:
1868 683 605
BsmI GAATG_Cn'
Cuts at: 0 537 1532 3156
Size: 537 995 1624
Fragments arranged by size:
1624 995 537
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2812 3156
Size: 2812 344
Bsp1286I G_dGCh'C
Cuts at: 0 228 511 605 1227 1288 1456 2040 3156
Size: 228 283 94 622 61 168 584 1116
Fragments arranged by size:
1116 622 584 283 228 168 94 61
BspEI T'CCGG_A
Cuts at: 0 2129 3156
Size: 2129 1027
BspHI T'CATG_A
Cuts at: 0 1748 3156
Size: 1748 1408
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 751 1252 3117 3156
Size: 751 501 1865 39
Fragments arranged by size:
1865 751 501 39
BsrBI CCG'CTC
Cuts at: 0 2939 3156
Size: 2939 217
BsrDI GCAATG_nn'
Cuts at: 0 447 1017 1730 3156
Size: 447 570 713 1426
Fragments arranged by size:
1426 713 570 447
BssSI C'ACGA_G
Cuts at: 0 31 3156
Size: 31 3125
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3120 3156
Size: 3120 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2547 2733 2868 3156
Size: 2547 186 135 288
Fragments arranged by size:
2547 288 186 135
BstYI r'GATC_y
Cuts at: 0 1418 1467 3156
Size: 1418 49 1689
Fragments arranged by size:
1689 1418 49
BtgI C'CryG_G
Cuts at: 0 744 1386 3029 3156
Size: 744 642 1643 127
Fragments arranged by size:
1643 744 642 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1083 2324 2361 3156
Size: 1083 1241 37 795
Fragments arranged by size:
1241 1083 795 37
BtsI GCAGTG_nn'
Cuts at: 0 243 586 2236 3156
Size: 243 343 1650 920
Fragments arranged by size:
1650 920 343 243
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 134 169 3156
Size: 134 35 2987
Fragments arranged by size:
2987 134 35
DraI TTT'AAA
Cuts at: 0 893 2444 2813 3156
Size: 893 1551 369 343
Fragments arranged by size:
1551 893 369 343
EaeI y'GGCC_r
Cuts at: 0 1685 2339 2973 3156
Size: 1685 654 634 183
Fragments arranged by size:
1685 654 634 183
EarI CTCTTCn'nnn_
Cuts at: 0 226 483 2108 3156
Size: 226 257 1625 1048
Fragments arranged by size:
1625 1048 257 226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 785 1235 2132 2151 2584 2672 2755
Size: 250 535 450 897 19 433 88 83
Cuts at: 2755 2951 2978 3156
Size: 196 27 178
Fragments arranged by size:
897 535 450 433 250 196 178 88
83 27 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2872 3156
Size: 2872 284
EcoO109I rG'GnC_Cy
Cuts at: 0 96 1224 1332 2028 2036 2037 2918 3156
Size: 96 1128 108 696 8 1 881 238
Fragments arranged by size:
1128 881 696 238 108 96 8 1
EcoRI G'AATT_C
Cuts at: 0 136 3156
Size: 136 3020
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2592 3156
Size: 2592 564
HaeII r_GCGC'y
Cuts at: 0 2661 2827 2945 3111 3156
Size: 2661 166 118 166 45
Fragments arranged by size:
2661 166 166 118 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1464 1496 1765 1797 2246 2269 2278 2301
Size: 1464 32 269 32 449 23 9 23
Cuts at: 2301 3156
Size: 855
Fragments arranged by size:
1464 855 449 269 32 32 23 23
9
HincII GTy'rAC
Cuts at: 0 12 957 1368 3156
Size: 12 945 411 1788
Fragments arranged by size:
1788 945 411 12
HindIII A'AGCT_T
Cuts at: 0 4 3156
Size: 4 3152
KasI G'GCGC_C
Cuts at: 0 2823 2941 3156
Size: 2823 118 215
Fragments arranged by size:
2823 215 118
MfeI C'AATT_G
Cuts at: 0 249 2541 3156
Size: 249 2292 615
Fragments arranged by size:
2292 615 249
MscI TGG'CCA
Cuts at: 0 1687 2341 3156
Size: 1687 654 815
Fragments arranged by size:
1687 815 654
MslI CAynn'nnrTG
Cuts at: 0 754 1699 1828 3156
Size: 754 945 129 1328
Fragments arranged by size:
1328 945 754 129
MspA1I CmG'CkG
Cuts at: 0 2503 3156
Size: 2503 653
NarI GG'CG_CC
Cuts at: 0 2824 2942 3156
Size: 2824 118 214
Fragments arranged by size:
2824 214 118
NcoI C'CATG_G
Cuts at: 0 744 3029 3156
Size: 744 2285 127
Fragments arranged by size:
2285 744 127
NruI TCG'CGA
Cuts at: 0 2380 3156
Size: 2380 776
NspI r_CATG'y
Cuts at: 0 526 1374 1491 2355 3156
Size: 526 848 117 864 801
Fragments arranged by size:
864 848 801 526 117
PasI CC'CwG_GG
Cuts at: 0 504 1916 3098 3156
Size: 504 1412 1182 58
Fragments arranged by size:
1412 1182 504 58
PciI A'CATG_T
Cuts at: 0 1370 1487 2351 3156
Size: 1370 117 864 805
Fragments arranged by size:
1370 864 805 117
PflMI CCAn_nnn'nTGG
Cuts at: 0 106 2395 2433 2991 3156
Size: 106 2289 38 558 165
Fragments arranged by size:
2289 558 165 106 38
PfoI T'CCnGG_A
Cuts at: 0 2955 3156
Size: 2955 201
PmlI CAC'GTG
Cuts at: 0 2048 3156
Size: 2048 1108
PpuMI rG'GwC_Cy
Cuts at: 0 2028 3156
Size: 2028 1128
PspOMI G'GGCC_C
Cuts at: 0 1223 2036 3156
Size: 1223 813 1120
Fragments arranged by size:
1223 1120 813
PstI C_TGCA'G
Cuts at: 0 593 3156
Size: 593 2563
PvuI CG_AT'CG
Cuts at: 0 2899 3156
Size: 2899 257
PvuII CAG'CTG
Cuts at: 0 2503 3156
Size: 2503 653
SapI GCTCTTCn'nnn_
Cuts at: 0 226 483 3156
Size: 226 257 2673
Fragments arranged by size:
2673 257 226
ScaI AGT'ACT
Cuts at: 0 2104 3156
Size: 2104 1052
SfcI C'TryA_G
Cuts at: 0 589 3156
Size: 589 2567
SfoI GGC'GCC
Cuts at: 0 2825 2943 3156
Size: 2825 118 213
Fragments arranged by size:
2825 213 118
SmaI CCC'GGG
Cuts at: 0 1362 3156
Size: 1362 1794
SmlI C'TyrA_G
Cuts at: 0 406 2909 3156
Size: 406 2503 247
Fragments arranged by size:
2503 406 247
SphI G_CATG'C
Cuts at: 0 526 3156
Size: 526 2630
StyI C'CwwG_G
Cuts at: 0 744 789 1669 2427 2726 3029 3156
Size: 744 45 880 758 299 303 127
Fragments arranged by size:
880 758 744 303 299 127 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1647 3156
Size: 1647 1509
TatI w'GTAC_w
Cuts at: 0 2102 2523 2603 3156
Size: 2102 421 80 553
Fragments arranged by size:
2102 553 421 80
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3017 3049 3156
Size: 3017 32 107
Fragments arranged by size:
3017 107 32
Tth111I GACn'n_nGTC
Cuts at: 0 1171 1779 3156
Size: 1171 608 1377
Fragments arranged by size:
1377 1171 608
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 501 2789 3036 3156
Size: 501 2288 247 120
Fragments arranged by size:
2288 501 247 120
XmaI C'CCGG_G
Cuts at: 0 1360 3156
Size: 1360 1796
XmnI GAAnn'nnTTC
Cuts at: 0 537 3156
Size: 537 2619
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AlwNI
ApaI ApoI AvaI BanI BanII BbeI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BssSI BstAPI BstXI
BstYI BtgI BtgZI BtsI CspCI DraI EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII KasI MfeI MscI MslI MspA1I NarI NcoI
NruI NspI PasI PciI PflMI PfoI PmlI PpuMI
PspOMI PstI PvuI PvuII SapI ScaI SfcI SfoI
SmaI SmlI SphI StyI TaqII TatI TstI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI
BfrBI BmtI BsaBI BsiWI BsrFI BsrGI BssHII BstBI
BstEII BstZ17I Bsu36I ClaI DraIII DrdI EagI EciI
EcoICRI EcoRV FseI FspI FspAI HpaI KpnI MluI
MmeI NaeI NdeI NgoMIV NheI NotI NsiI PacI
PmeI PpiI PshAI PsiI PspXI PsrI RsrII SacI
SacII SalI SanDI SbfI SexAI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI
ZraI