Restriction map for
pF1KA0090
(Linear) (Six-base) MAPSORT of: KIAA0090.seq Check: 6902 from: 1 to: 2976
KIAA0090.orf
With 182 enzymes: SgfI *
September 25, 2009 11:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2585 2976
Size: 2585 391
AccI GT'mk_AC
Cuts at: 0 65 2837 2976
Size: 65 2772 139
Fragments arranged by size:
2772 139 65
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 564 1061 1873 2445 2884 2976
Size: 457 107 497 812 572 439 92
Fragments arranged by size:
812 572 497 457 439 107 92
AflIII A'CryG_T
Cuts at: 0 2882 2976
Size: 2882 94
AhdI GACnn_n'nnGTC
Cuts at: 0 1779 2976
Size: 1779 1197
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 611 643 2976
Size: 611 32 2333
Fragments arranged by size:
2333 611 32
AlwNI CAG_nnn'CTG
Cuts at: 0 666 2976
Size: 666 2310
ApaI G_GGCC'C
Cuts at: 0 564 867 2976
Size: 564 303 2109
Fragments arranged by size:
2109 564 303
ApaLI G'TGCA_C
Cuts at: 0 1755 2976
Size: 1755 1221
ApoI r'AATT_y
Cuts at: 0 136 198 799 1411 1582 2976
Size: 136 62 601 612 171 1394
Fragments arranged by size:
1394 612 601 171 136 62
AvaI C'yCGr_G
Cuts at: 0 717 821 860 2644 2775 2831 2976
Size: 717 104 39 1784 131 56 145
Fragments arranged by size:
1784 717 145 131 104 56 39
BamHI G'GATC_C
Cuts at: 0 148 2631 2976
Size: 148 2483 345
Fragments arranged by size:
2483 345 148
BanI G'GyrC_C
Cuts at: 0 224 476 1654 2976
Size: 224 252 1178 1322
Fragments arranged by size:
1322 1178 252 224
BanII G_rGCy'C
Cuts at: 0 564 724 867 2426 2976
Size: 564 160 143 1559 550
Fragments arranged by size:
1559 564 550 160 143
BbeI G_GCGC'C
Cuts at: 0 228 2976
Size: 228 2748
BbsI GAAGACnn'nnnn_
Cuts at: 0 77 448 994 1797 2976
Size: 77 371 546 803 1179
Fragments arranged by size:
1179 803 546 371 77
BclI T'GATC_A
Cuts at: 0 290 2927 2976
Size: 290 2637 49
Fragments arranged by size:
2637 290 49
BglI GCCn_nnn'nGGC
Cuts at: 0 1206 2539 2976
Size: 1206 1333 437
Fragments arranged by size:
1333 1206 437
BglII A'GATC_T
Cuts at: 0 215 2976
Size: 215 2761
Bme1580I G_kGCm'C
Cuts at: 0 244 479 564 867 1392 1759 2437 2976
Size: 244 235 85 303 525 367 678 539
Fragments arranged by size:
678 539 525 367 303 244 235 85
BmrI ACTGGGnnnn_n'
Cuts at: 0 218 363 841 861 989 1394 1840 1879
Size: 218 145 478 20 128 405 446 39
Cuts at: 1879 2303 2373 2976
Size: 424 70 603
Fragments arranged by size:
603 478 446 424 405 218 145 128
70 39 20
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1211 1665 2976
Size: 1211 454 1311
Fragments arranged by size:
1311 1211 454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 692 769 1576 2440 2482 2618 2678
Size: 453 239 77 807 864 42 136 60
Cuts at: 2678 2803 2976
Size: 125 173
Fragments arranged by size:
864 807 453 239 173 136 125 77
60 42
Bpu10I CC'TnA_GC
Cuts at: 0 1202 2976
Size: 1202 1774
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 2976
Size: 466 2510
BsaAI yAC'GTr
Cuts at: 0 2883 2976
Size: 2883 93
BsaHI Gr'CG_yC
Cuts at: 0 225 2288 2976
Size: 225 2063 688
Fragments arranged by size:
2063 688 225
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 205 235 440 470 611 641 716 746
Size: 205 30 205 30 141 30 75 30
Cuts at: 746 2976
Size: 2230
Fragments arranged by size:
2230 205 205 141 75 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1348 1689 2301 2686 2976
Size: 1348 341 612 385 290
Fragments arranged by size:
1348 612 385 341 290
BseYI C'CCAG_C
Cuts at: 0 836 1437 2480 2976
Size: 836 601 1043 496
Fragments arranged by size:
1043 836 601 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 862 1285 2338 2976
Size: 259 603 423 1053 638
Fragments arranged by size:
1053 638 603 423 259
BsiEI CG_ry'CG
Cuts at: 0 59 2976
Size: 59 2917
BsiHKAI G_wGCw'C
Cuts at: 0 724 1759 2150 2192 2239 2426 2976
Size: 724 1035 391 42 47 187 550
Fragments arranged by size:
1035 724 550 391 187 47 42
BsmI GAATG_Cn'
Cuts at: 0 2352 2756 2976
Size: 2352 404 220
Fragments arranged by size:
2352 404 220
BsmBI CGTCTCn'nnnn_
Cuts at: 0 745 1469 2976
Size: 745 724 1507
Fragments arranged by size:
1507 745 724
Bsp1286I G_dGCh'C
Cuts at: 0 244 479 564 724 867 1392 1759 2150
Size: 244 235 85 160 143 525 367 391
Cuts at: 2150 2192 2239 2426 2437 2976
Size: 42 47 187 11 539
Fragments arranged by size:
539 525 391 367 244 235 187 160
143 85 47 42 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 624 2222 2585 2976
Size: 624 1598 363 391
Fragments arranged by size:
1598 624 391 363
BsrBI CCG'CTC
Cuts at: 0 1190 1206 2976
Size: 1190 16 1770
Fragments arranged by size:
1770 1190 16
BsrGI T'GTAC_A
Cuts at: 0 2700 2976
Size: 2700 276
BssSI C'ACGA_G
Cuts at: 0 1132 2976
Size: 1132 1844
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1875 2976
Size: 1875 1101
BstBI TT'CG_AA
Cuts at: 0 2055 2976
Size: 2055 921
BstXI CCAn_nnnn'nTGG
Cuts at: 0 159 391 849 2217 2796 2976
Size: 159 232 458 1368 579 180
Fragments arranged by size:
1368 579 458 232 180 159
BstYI r'GATC_y
Cuts at: 0 148 215 773 2061 2631 2648 2976
Size: 148 67 558 1288 570 17 328
Fragments arranged by size:
1288 570 558 328 148 67 17
Bsu36I CC'TnA_GG
Cuts at: 0 1620 2618 2976
Size: 1620 998 358
Fragments arranged by size:
1620 998 358
BtgI C'CryG_G
Cuts at: 0 59 652 2222 2534 2976
Size: 59 593 1570 312 442
Fragments arranged by size:
1570 593 442 312 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 411 2976
Size: 411 2565
BtsI GCAGTG_nn'
Cuts at: 0 778 2148 2190 2976
Size: 778 1370 42 786
Fragments arranged by size:
1370 786 778 42
DraI TTT'AAA
Cuts at: 0 1707 2976
Size: 1707 1269
EaeI y'GGCC_r
Cuts at: 0 56 1571 2219 2976
Size: 56 1515 648 757
Fragments arranged by size:
1515 757 648 56
EagI C'GGCC_G
Cuts at: 0 56 2976
Size: 56 2920
EcoICRI GAG'CTC
Cuts at: 0 722 2424 2976
Size: 722 1702 552
Fragments arranged by size:
1702 722 552
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 457 564 692 769 1061 1576 1873
Size: 453 4 107 128 77 292 515 297
Cuts at: 1873 2440 2445 2482 2618 2678 2803 2884 2976
Size: 567 5 37 136 60 125 81 92
Fragments arranged by size:
567 515 453 297 292 136 128 125
107 92 81 77 60 37 5 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2617 2976
Size: 2617 359
EcoO109I rG'GnC_Cy
Cuts at: 0 345 561 825 2335 2493 2976
Size: 345 216 264 1510 158 483
Fragments arranged by size:
1510 483 345 264 216 158
EcoRI G'AATT_C
Cuts at: 0 1582 2976
Size: 1582 1394
EcoRV GAT'ATC
Cuts at: 0 2047 2976
Size: 2047 929
HaeII r_GCGC'y
Cuts at: 0 228 1860 2976
Size: 228 1632 1116
Fragments arranged by size:
1632 1116 228
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 299 331 706 738 1260 1292 1765 1797
Size: 299 32 375 32 522 32 473 32
Cuts at: 1797 2730 2762 2976
Size: 933 32 214
Fragments arranged by size:
933 522 473 375 299 214 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 234 2976
Size: 234 2742
HindIII A'AGCT_T
Cuts at: 0 1627 2976
Size: 1627 1349
KasI G'GCGC_C
Cuts at: 0 224 2976
Size: 224 2752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 840 994 2976
Size: 840 154 1982
Fragments arranged by size:
1982 840 154
MscI TGG'CCA
Cuts at: 0 1573 2976
Size: 1573 1403
MspA1I CmG'CkG
Cuts at: 0 61 2112 2484 2976
Size: 61 2051 372 492
Fragments arranged by size:
2051 492 372 61
NarI GG'CG_CC
Cuts at: 0 225 2976
Size: 225 2751
NcoI C'CATG_G
Cuts at: 0 2534 2976
Size: 2534 442
NotI GC'GGCC_GC
Cuts at: 0 56 2976
Size: 56 2920
PasI CC'CwG_GG
Cuts at: 0 2160 2976
Size: 2160 816
PflMI CCAn_nnn'nTGG
Cuts at: 0 741 1664 2976
Size: 741 923 1312
Fragments arranged by size:
1312 923 741
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 611 643 711 743 1716 1748 2976
Size: 611 32 68 32 973 32 1228
Fragments arranged by size:
1228 973 611 68 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 825 2493 2976
Size: 825 1668 483
Fragments arranged by size:
1668 825 483
PspOMI G'GGCC_C
Cuts at: 0 560 863 2976
Size: 560 303 2113
Fragments arranged by size:
2113 560 303
PspXI vC'TCGA_Gb
Cuts at: 0 2831 2976
Size: 2831 145
PstI C_TGCA'G
Cuts at: 0 410 662 785 911 1002 1298 2976
Size: 410 252 123 126 91 296 1678
Fragments arranged by size:
1678 410 296 252 126 123 91
PvuII CAG'CTG
Cuts at: 0 2484 2976
Size: 2484 492
SacI G_AGCT'C
Cuts at: 0 724 2426 2976
Size: 724 1702 550
Fragments arranged by size:
1702 724 550
SacII CC_GC'GG
Cuts at: 0 62 2976
Size: 62 2914
SbfI CC_TGCA'GG
Cuts at: 0 410 2976
Size: 410 2566
ScaI AGT'ACT
Cuts at: 0 2380 2976
Size: 2380 596
SfcI C'TryA_G
Cuts at: 0 406 658 781 907 998 1294 2976
Size: 406 252 123 126 91 296 1682
Fragments arranged by size:
1682 406 296 252 126 123 91
SfoI GGC'GCC
Cuts at: 0 226 2976
Size: 226 2750
SmaI CCC'GGG
Cuts at: 0 823 862 2976
Size: 823 39 2114
Fragments arranged by size:
2114 823 39
SmlI C'TyrA_G
Cuts at: 0 481 2831 2976
Size: 481 2350 145
Fragments arranged by size:
2350 481 145
StyI C'CwwG_G
Cuts at: 0 131 1907 2390 2534 2976
Size: 131 1776 483 144 442
Fragments arranged by size:
1776 483 442 144 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 477 2355 2976
Size: 477 1878 621
Fragments arranged by size:
1878 621 477
TatI w'GTAC_w
Cuts at: 0 2106 2378 2700 2976
Size: 2106 272 322 276
Fragments arranged by size:
2106 322 276 272
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 205 237 272 304 440 472 716 748
Size: 205 32 35 32 136 32 244 32
Cuts at: 748 1935 1967 2140 2172 2976
Size: 1187 32 173 32 804
Fragments arranged by size:
1187 804 244 205 173 136 35 32
32 32 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2532 2976
Size: 2532 444
XhoI C'TCGA_G
Cuts at: 0 2831 2976
Size: 2831 145
XmaI C'CCGG_G
Cuts at: 0 821 860 2976
Size: 821 39 2116
Fragments arranged by size:
2116 821 39
XmnI GAAnn'nnTTC
Cuts at: 0 1791 2976
Size: 1791 1185
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AloI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BclI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV HaeII Hin4I HincII HindIII KasI MmeI MscI
MspA1I NarI NcoI NotI PasI PflMI PpiI PpuMI
PspOMI PspXI PstI PvuII SacI SacII SbfI ScaI
SfcI SfoI SmaI SmlI StyI TaqII TatI TstI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AlfI
AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI
BfrBI BlpI BmgBI BmtI BsaI BsaBI BsaWI BsiWI
BspEI BspHI BsrDI BsrFI BssHII BstEII BstZ17I ClaI
CspCI DraIII DrdI EarI EciI FalI FseI FspI
FspAI HpaI KpnI MfeI MluI MslI NaeI NdeI
NgoMIV NheI NruI NsiI NspI PacI PciI PfoI
PmeI PmlI PshAI PsiI PsrI PvuI RsrII SalI
SanDI SapI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI ZraI