Restriction map for
pF1KSDA0086
(Linear) (Six-base) MAPSORT of: KIAA0086.seq Check: 3787 from: 1 to: 3120
KIAA0086.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 582 1242 1596 3120
Size: 582 660 354 1524
Fragments arranged by size:
1524 660 582 354
AclI AA'CG_TT
Cuts at: 0 2469 3120
Size: 2469 651
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 861 1075 2003 2430 3040 3120
Size: 746 115 214 928 427 610 80
Fragments arranged by size:
928 746 610 427 214 115 80
AfeI AGC'GCT
Cuts at: 0 3004 3120
Size: 3004 116
AflII C'TTAA_G
Cuts at: 0 1624 3120
Size: 1624 1496
AflIII A'CryG_T
Cuts at: 0 324 326 2098 2768 3120
Size: 324 2 1772 670 352
Fragments arranged by size:
1772 670 352 324 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1530 3120
Size: 1530 1590
AlwNI CAG_nnn'CTG
Cuts at: 0 884 1981 2389 3018 3120
Size: 884 1097 408 629 102
Fragments arranged by size:
1097 884 629 408 102
ApoI r'AATT_y
Cuts at: 0 240 852 1300 1831 1923 2963 3120
Size: 240 612 448 531 92 1040 157
Fragments arranged by size:
1040 612 531 448 240 157 92
AseI AT'TA_AT
Cuts at: 0 1970 2747 2810 3120
Size: 1970 777 63 310
Fragments arranged by size:
1970 777 310 63
AvaI C'yCGr_G
Cuts at: 0 1100 3120
Size: 1100 2020
BanI G'GyrC_C
Cuts at: 0 3055 3120
Size: 3055 65
BbsI GAAGACnn'nnnn_
Cuts at: 0 14 29 466 632 926 2004 2649 3120
Size: 14 15 437 166 294 1078 645 471
Fragments arranged by size:
1078 645 471 437 294 166 15 14
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 391 425 3120
Size: 391 34 2695
Fragments arranged by size:
2695 391 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 352 3120
Size: 352 2768
BclI T'GATC_A
Cuts at: 0 549 2205 3120
Size: 549 1656 915
Fragments arranged by size:
1656 915 549
BglI GCCn_nnn'nGGC
Cuts at: 0 2590 3120
Size: 2590 530
Bme1580I G_kGCm'C
Cuts at: 0 197 3058 3120
Size: 197 2861 62
Fragments arranged by size:
2861 197 62
BmtI G_CTAG'C
Cuts at: 0 532 3120
Size: 532 2588
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 533 3120
Size: 533 2587
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 3120
Size: 275 2845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 3120
Size: 646 2474
BsaAI yAC'GTr
Cuts at: 0 327 3120
Size: 327 2793
BsaBI GATnn'nnATC
Cuts at: 0 2372 3120
Size: 2372 748
BsaHI Gr'CG_yC
Cuts at: 0 611 3120
Size: 611 2509
BsaWI w'CCGG_w
Cuts at: 0 945 2564 3120
Size: 945 1619 556
Fragments arranged by size:
1619 945 556
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1575 1605 3120
Size: 1575 30 1515
Fragments arranged by size:
1575 1515 30
BseYI C'CCAG_C
Cuts at: 0 2458 3120
Size: 2458 662
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2793 3120
Size: 2793 327
BsiHKAI G_wGCw'C
Cuts at: 0 3076 3120
Size: 3076 44
BsmI GAATG_Cn'
Cuts at: 0 1488 3120
Size: 1488 1632
BsmBI CGTCTCn'nnnn_
Cuts at: 0 722 3120
Size: 722 2398
Bsp1286I G_dGCh'C
Cuts at: 0 197 3058 3076 3120
Size: 197 2861 18 44
Fragments arranged by size:
2861 197 44 18
BspHI T'CATG_A
Cuts at: 0 927 2393 3120
Size: 927 1466 727
Fragments arranged by size:
1466 927 727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 199 582 1242 1596 3120
Size: 199 383 660 354 1524
Fragments arranged by size:
1524 660 383 354 199
BsrDI GCAATG_nn'
Cuts at: 0 859 2314 2666 3120
Size: 859 1455 352 454
Fragments arranged by size:
1455 859 454 352
BsrFI r'CCGG_y
Cuts at: 0 2128 3120
Size: 2128 992
BstYI r'GATC_y
Cuts at: 0 2454 3120
Size: 2454 666
BtsI GCAGTG_nn'
Cuts at: 0 1798 1927 2579 3120
Size: 1798 129 652 541
Fragments arranged by size:
1798 652 541 129
ClaI AT'CG_AT
Cuts at: 0 935 3120
Size: 935 2185
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1220 1255 3120
Size: 1220 35 1865
Fragments arranged by size:
1865 1220 35
DraI TTT'AAA
Cuts at: 0 705 1032 1562 3120
Size: 705 327 530 1558
Fragments arranged by size:
1558 705 530 327
DrdI GACnn_nn'nnGTC
Cuts at: 0 2491 3120
Size: 2491 629
EarI CTCTTCn'nnn_
Cuts at: 0 1376 1706 1945 2480 3120
Size: 1376 330 239 535 640
Fragments arranged by size:
1376 640 535 330 239
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 746 861 1075 2003 2430 3040 3120
Size: 275 471 115 214 928 427 610 80
Fragments arranged by size:
928 610 471 427 275 214 115 80
EcoNI CCTnn'n_nnAGG
Cuts at: 0 578 3120
Size: 578 2542
EcoO109I rG'GnC_Cy
Cuts at: 0 1784 3120
Size: 1784 1336
EcoRI G'AATT_C
Cuts at: 0 240 2963 3120
Size: 240 2723 157
Fragments arranged by size:
2723 240 157
EcoRV GAT'ATC
Cuts at: 0 933 3120
Size: 933 2187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 214 656 1387 1444 1661 3120
Size: 214 442 731 57 217 1459
Fragments arranged by size:
1459 731 442 217 214 57
HaeII r_GCGC'y
Cuts at: 0 3006 3120
Size: 3006 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 589 621 732 764 1944 1976 3120
Size: 589 32 111 32 1180 32 1144
Fragments arranged by size:
1180 1144 589 111 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1249 2284 3120
Size: 1249 1035 836
Fragments arranged by size:
1249 1035 836
MslI CAynn'nnrTG
Cuts at: 0 411 3120
Size: 411 2709
MspA1I CmG'CkG
Cuts at: 0 1019 3120
Size: 1019 2101
NdeI CA'TA_TG
Cuts at: 0 2498 3120
Size: 2498 622
NheI G'CTAG_C
Cuts at: 0 528 3120
Size: 528 2592
NspI r_CATG'y
Cuts at: 0 421 1205 1511 2102 2614 2700 2772 3120
Size: 421 784 306 591 512 86 72 348
Fragments arranged by size:
784 591 512 421 348 306 86 72
PciI A'CATG_T
Cuts at: 0 2098 2768 3120
Size: 2098 670 352
Fragments arranged by size:
2098 670 352
PflMI CCAn_nnn'nTGG
Cuts at: 0 76 3120
Size: 76 3044
PfoI T'CCnGG_A
Cuts at: 0 291 3120
Size: 291 2829
PmlI CAC'GTG
Cuts at: 0 327 3120
Size: 327 2793
PpuMI rG'GwC_Cy
Cuts at: 0 1784 3120
Size: 1784 1336
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2118 2150 3120
Size: 2118 32 970
Fragments arranged by size:
2118 970 32
PstI C_TGCA'G
Cuts at: 0 1519 1934 3120
Size: 1519 415 1186
Fragments arranged by size:
1519 1186 415
PvuII CAG'CTG
Cuts at: 0 1019 3120
Size: 1019 2101
ScaI AGT'ACT
Cuts at: 0 1182 1845 3120
Size: 1182 663 1275
Fragments arranged by size:
1275 1182 663
SfcI C'TryA_G
Cuts at: 0 303 1515 1930 2722 2882 3120
Size: 303 1212 415 792 160 238
Fragments arranged by size:
1212 792 415 303 238 160
SmlI C'TyrA_G
Cuts at: 0 625 1624 3120
Size: 625 999 1496
Fragments arranged by size:
1496 999 625
SpeI A'CTAG_T
Cuts at: 0 807 3120
Size: 807 2313
SspI AAT'ATT
Cuts at: 0 90 1610 3088 3120
Size: 90 1520 1478 32
Fragments arranged by size:
1520 1478 90 32
StyI C'CwwG_G
Cuts at: 0 198 1652 3120
Size: 198 1454 1468
Fragments arranged by size:
1468 1454 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2327 3120
Size: 2327 793
TatI w'GTAC_w
Cuts at: 0 1180 1204 1582 1843 2504 3120
Size: 1180 24 378 261 661 616
Fragments arranged by size:
1180 661 616 378 261 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1927 1959 3120
Size: 1927 32 1161
Fragments arranged by size:
1927 1161 32
Tth111I GACn'n_nGTC
Cuts at: 0 603 1043 3120
Size: 603 440 2077
Fragments arranged by size:
2077 603 440
XmnI GAAnn'nnTTC
Cuts at: 0 1090 2656 2949 3120
Size: 1090 1566 293 171
Fragments arranged by size:
1566 1090 293 171
Enzymes that do cut:
AarI AclI AcuI AfeI AflII AflIII AlfI AlwNI
ApoI AseI AvaI BanI BbsI BcgI BciVI BclI
BglI Bme1580I BmtI BplI BpmI BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrFI BstYI BtsI ClaI
CspCI DraI DrdI EarI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HindIII MslI MspA1I NdeI
NheI NspI PciI PflMI PfoI PmlI PpuMI PsrI
PstI PvuII ScaI SfcI SmlI SpeI SspI StyI
TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AatII AccI Acc65I AgeI AhdI AleI AloI ApaI
ApaLI AscI AsiSI AvrII BaeI BamHI BanII BbeI
BbvCI BfrBI BglII BlpI BmgBI BmrI Bpu10I BsaI
BseRI BsiEI BsiWI BspEI BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
DraIII EaeI EagI EciI EcoICRI FseI FspI FspAI
HincII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NcoI NgoMIV NotI NruI NsiI PacI
PasI PmeI PpiI PshAI PsiI PspOMI PspXI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
StuI SwaI XbaI XcmI XhoI XmaI ZraI