Restriction map for
pF1KA0082
(Linear) (Six-base) MAPSORT of: KIAA0082.seq Check: 2974 from: 1 to: 2505
KIAA0082.orf
With 182 enzymes: SgfI *
October 24, 2006 15:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 450 2505
Size: 450 2055
AatII G_ACGT'C
Cuts at: 0 1430 2477 2505
Size: 1430 1047 28
Fragments arranged by size:
1430 1047 28
AccI GT'mk_AC
Cuts at: 0 1250 2505
Size: 1250 1255
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 1366 1692 1900 2505
Size: 282 1084 326 208 605
Fragments arranged by size:
1084 605 326 282 208
AhdI GACnn_n'nnGTC
Cuts at: 0 2460 2505
Size: 2460 45
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1162 2440 2505
Size: 1162 1278 65
Fragments arranged by size:
1278 1162 65
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1405 1437 2505
Size: 1405 32 1068
Fragments arranged by size:
1405 1068 32
AlwNI CAG_nnn'CTG
Cuts at: 0 414 2505
Size: 414 2091
ApaI G_GGCC'C
Cuts at: 0 913 2505
Size: 913 1592
ApoI r'AATT_y
Cuts at: 0 491 1038 1471 2036 2053 2505
Size: 491 547 433 565 17 452
Fragments arranged by size:
565 547 491 452 433 17
AvaI C'yCGr_G
Cuts at: 0 656 1895 2505
Size: 656 1239 610
Fragments arranged by size:
1239 656 610
BamHI G'GATC_C
Cuts at: 0 1619 2505
Size: 1619 886
BanI G'GyrC_C
Cuts at: 0 2002 2185 2505
Size: 2002 183 320
Fragments arranged by size:
2002 320 183
BanII G_rGCy'C
Cuts at: 0 446 651 913 2179 2270 2505
Size: 446 205 262 1266 91 235
Fragments arranged by size:
1266 446 262 235 205 91
BbsI GAAGACnn'nnnn_
Cuts at: 0 681 1888 2505
Size: 681 1207 617
Fragments arranged by size:
1207 681 617
BbvCI CC'TCA_GC
Cuts at: 0 79 1135 2505
Size: 79 1056 1370
Fragments arranged by size:
1370 1056 79
BclI T'GATC_A
Cuts at: 0 1454 2505
Size: 1454 1051
BfrBI ATG'CAT
Cuts at: 0 208 2505
Size: 208 2297
BglI GCCn_nnn'nGGC
Cuts at: 0 842 2082 2505
Size: 842 1240 423
Fragments arranged by size:
1240 842 423
BglII A'GATC_T
Cuts at: 0 1829 2505
Size: 1829 676
BlpI GC'TnA_GC
Cuts at: 0 803 2197 2505
Size: 803 1394 308
Fragments arranged by size:
1394 803 308
Bme1580I G_kGCm'C
Cuts at: 0 913 1693 2505
Size: 913 780 812
Fragments arranged by size:
913 812 780
BmgBI CAC'GTC
Cuts at: 0 89 2505
Size: 89 2416
BmrI ACTGGGnnnn_n'
Cuts at: 0 440 2505
Size: 440 2065
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1041 1300 1592 2505
Size: 1041 259 292 913
Fragments arranged by size:
1041 913 292 259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 1468 1765 2163 2505
Size: 949 519 297 398 342
Fragments arranged by size:
949 519 398 342 297
Bpu10I CC'TnA_GC
Cuts at: 0 73 79 1135 2505
Size: 73 6 1056 1370
Fragments arranged by size:
1370 1056 73 6
BsaI GGTCTCn'nnnn_
Cuts at: 0 160 1457 2505
Size: 160 1297 1048
Fragments arranged by size:
1297 1048 160
BsaBI GATnn'nnATC
Cuts at: 0 1702 2164 2505
Size: 1702 462 341
Fragments arranged by size:
1702 462 341
BsaHI Gr'CG_yC
Cuts at: 0 1427 2474 2505
Size: 1427 1047 31
Fragments arranged by size:
1427 1047 31
BsaWI w'CCGG_w
Cuts at: 0 1184 2505
Size: 1184 1321
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2464 2494 2505
Size: 2464 30 11
Fragments arranged by size:
2464 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 21 99 698 2505
Size: 21 78 599 1807
Fragments arranged by size:
1807 599 78 21
BsiHKAI G_wGCw'C
Cuts at: 0 651 2505
Size: 651 1854
BsmI GAATG_Cn'
Cuts at: 0 28 208 2417 2505
Size: 28 180 2209 88
Fragments arranged by size:
2209 180 88 28
BsmBI CGTCTCn'nnnn_
Cuts at: 0 273 2505
Size: 273 2232
Bsp1286I G_dGCh'C
Cuts at: 0 446 651 913 1693 2179 2270 2505
Size: 446 205 262 780 486 91 235
Fragments arranged by size:
780 486 446 262 235 205 91
BspEI T'CCGG_A
Cuts at: 0 1184 2505
Size: 1184 1321
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 450 1130 1262 2505
Size: 450 680 132 1243
Fragments arranged by size:
1243 680 450 132
BsrDI GCAATG_nn'
Cuts at: 0 716 2360 2505
Size: 716 1644 145
Fragments arranged by size:
1644 716 145
BsrFI r'CCGG_y
Cuts at: 0 2219 2390 2505
Size: 2219 171 115
Fragments arranged by size:
2219 171 115
BsrGI T'GTAC_A
Cuts at: 0 2117 2505
Size: 2117 388
BssSI C'ACGA_G
Cuts at: 0 1634 2505
Size: 1634 871
BstAPI GCAn_nnn'nTGC
Cuts at: 0 67 2505
Size: 67 2438
BstBI TT'CG_AA
Cuts at: 0 962 2505
Size: 962 1543
BstYI r'GATC_y
Cuts at: 0 1130 1619 1751 1829 2505
Size: 1130 489 132 78 676
Fragments arranged by size:
1130 676 489 132 78
BtgI C'CryG_G
Cuts at: 0 760 2234 2505
Size: 760 1474 271
Fragments arranged by size:
1474 760 271
BtsI GCAGTG_nn'
Cuts at: 0 26 607 1391 1723 1797 2505
Size: 26 581 784 332 74 708
Fragments arranged by size:
784 708 581 332 74 26
DraIII CAC_nnn'GTG
Cuts at: 0 594 1570 2505
Size: 594 976 935
Fragments arranged by size:
976 935 594
EaeI y'GGCC_r
Cuts at: 0 284 1845 2388 2505
Size: 284 1561 543 117
Fragments arranged by size:
1561 543 284 117
EarI CTCTTCn'nnn_
Cuts at: 0 369 572 629 964 1291 1384 2123 2401
Size: 369 203 57 335 327 93 739 278
Cuts at: 2401 2505
Size: 104
Fragments arranged by size:
739 369 335 327 278 203 104 93
57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1744 2505
Size: 1744 761
EcoICRI GAG'CTC
Cuts at: 0 649 2505
Size: 649 1856
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 949 1366 1468 1692 1765 1900 2163
Size: 282 667 417 102 224 73 135 263
Cuts at: 2163 2505
Size: 342
Fragments arranged by size:
667 417 342 282 263 224 135 102
73
EcoO109I rG'GnC_Cy
Cuts at: 0 909 910 1341 2505
Size: 909 1 431 1164
Fragments arranged by size:
1164 909 431 1
EcoRI G'AATT_C
Cuts at: 0 2036 2505
Size: 2036 469
EcoRV GAT'ATC
Cuts at: 0 1005 2505
Size: 1005 1500
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 312 601 823 2325 2505
Size: 312 289 222 1502 180
Fragments arranged by size:
1502 312 289 222 180
FspI TGC'GCA
Cuts at: 0 831 2505
Size: 831 1674
HaeII r_GCGC'y
Cuts at: 0 1174 2505
Size: 1174 1331
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 448 480 925 957 1512 1544 1648 1680
Size: 448 32 445 32 555 32 104 32
Cuts at: 1680 2446 2464 2478 2496 2505
Size: 766 18 14 18 9
Fragments arranged by size:
766 555 448 445 104 32 32 32
32 18 18 14 9
HincII GTy'rAC
Cuts at: 0 1431 2505
Size: 1431 1074
HindIII A'AGCT_T
Cuts at: 0 277 2292 2505
Size: 277 2015 213
Fragments arranged by size:
2015 277 213
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 1448 1632 1679 2135 2505
Size: 316 1132 184 47 456 370
Fragments arranged by size:
1132 456 370 316 184 47
MscI TGG'CCA
Cuts at: 0 286 2505
Size: 286 2219
MslI CAynn'nnrTG
Cuts at: 0 2104 2422 2491 2505
Size: 2104 318 69 14
Fragments arranged by size:
2104 318 69 14
MspA1I CmG'CkG
Cuts at: 0 762 1146 1407 1775 1862 2505
Size: 762 384 261 368 87 643
Fragments arranged by size:
762 643 384 368 261 87
NaeI GCC'GGC
Cuts at: 0 2392 2505
Size: 2392 113
NcoI C'CATG_G
Cuts at: 0 2234 2505
Size: 2234 271
NgoMIV G'CCGG_C
Cuts at: 0 2390 2505
Size: 2390 115
NsiI A_TGCA'T
Cuts at: 0 210 2505
Size: 210 2295
NspI r_CATG'y
Cuts at: 0 750 883 2505
Size: 750 133 1622
Fragments arranged by size:
1622 750 133
PfoI T'CCnGG_A
Cuts at: 0 1046 2505
Size: 1046 1459
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 228 260 1786 1818 2505
Size: 228 32 1526 32 687
Fragments arranged by size:
1526 687 228 32 32
PspOMI G'GGCC_C
Cuts at: 0 909 2505
Size: 909 1596
PstI C_TGCA'G
Cuts at: 0 1127 1520 2355 2505
Size: 1127 393 835 150
Fragments arranged by size:
1127 835 393 150
PvuII CAG'CTG
Cuts at: 0 1146 1407 2505
Size: 1146 261 1098
Fragments arranged by size:
1146 1098 261
SacI G_AGCT'C
Cuts at: 0 651 2505
Size: 651 1854
SacII CC_GC'GG
Cuts at: 0 763 2505
Size: 763 1742
SapI GCTCTTCn'nnn_
Cuts at: 0 572 2401 2505
Size: 572 1829 104
Fragments arranged by size:
1829 572 104
SbfI CC_TGCA'GG
Cuts at: 0 1127 2505
Size: 1127 1378
SfcI C'TryA_G
Cuts at: 0 1123 1509 1516 2351 2505
Size: 1123 386 7 835 154
Fragments arranged by size:
1123 835 386 154 7
SmaI CCC'GGG
Cuts at: 0 1897 2505
Size: 1897 608
SphI G_CATG'C
Cuts at: 0 883 2505
Size: 883 1622
SspI AAT'ATT
Cuts at: 0 1392 2505
Size: 1392 1113
StyI C'CwwG_G
Cuts at: 0 2234 2327 2465 2505
Size: 2234 93 138 40
Fragments arranged by size:
2234 138 93 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 184 2505
Size: 184 2321
TatI w'GTAC_w
Cuts at: 0 812 1259 2117 2505
Size: 812 447 858 388
Fragments arranged by size:
858 812 447 388
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 477 509 1950 1982 2505
Size: 477 32 1441 32 523
Fragments arranged by size:
1441 523 477 32 32
XmaI C'CCGG_G
Cuts at: 0 1895 2505
Size: 1895 610
XmnI GAAnn'nnTTC
Cuts at: 0 240 2305 2308 2505
Size: 240 2065 3 197
Fragments arranged by size:
2065 240 197 3
ZraI GAC'GTC
Cuts at: 0 1428 2475 2505
Size: 1428 1047 30
Fragments arranged by size:
1428 1047 30
Enzymes that do cut:
AarI AatII AccI AcuI AhdI AlfI AloI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtsI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI
PfoI PpiI PspOMI PstI PvuII SacI SacII SapI
SbfI SfcI SmaI SphI SspI StyI TaqII TatI
TstI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AleI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI
BmtI BpuEI BsaAI BseYI BsgI BsiEI BsiWI BspHI
BsrBI BssHII BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EagI EcoNI FseI FspAI HpaI
KasI KpnI MfeI MluI NarI NdeI NheI NotI
NruI PacI PasI PciI PflMI PmeI PmlI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SrfI StuI SwaI Tth111I XbaI XcmI XhoI