Restriction map for
pF1KSDA0080
(Linear) (Six-base) MAPSORT of: KIAA0080.seq Check: 8984 from: 1 to: 1293
KIAA0080.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AccI GT'mk_AC
Cuts at: 0 1007 1293
Size: 1007 286
AgeI A'CCGG_T
Cuts at: 0 42 970 1293
Size: 42 928 323
Fragments arranged by size:
928 323 42
AlwNI CAG_nnn'CTG
Cuts at: 0 301 717 1293
Size: 301 416 576
Fragments arranged by size:
576 416 301
ApaLI G'TGCA_C
Cuts at: 0 1056 1293
Size: 1056 237
AvaI C'yCGr_G
Cuts at: 0 758 1293
Size: 758 535
BamHI G'GATC_C
Cuts at: 0 475 1293
Size: 475 818
BanI G'GyrC_C
Cuts at: 0 786 1293
Size: 786 507
BanII G_rGCy'C
Cuts at: 0 403 884 1252 1293
Size: 403 481 368 41
Fragments arranged by size:
481 403 368 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 637 655 1293
Size: 637 18 638
Fragments arranged by size:
638 637 18
BbvCI CC'TCA_GC
Cuts at: 0 205 739 1293
Size: 205 534 554
Fragments arranged by size:
554 534 205
BlpI GC'TnA_GC
Cuts at: 0 310 1293
Size: 310 983
Bme1580I G_kGCm'C
Cuts at: 0 1060 1200 1293
Size: 1060 140 93
Fragments arranged by size:
1060 140 93
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 898 1293
Size: 898 395
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 868 1251 1293
Size: 437 431 383 42
Fragments arranged by size:
437 431 383 42
Bpu10I CC'TnA_GC
Cuts at: 0 205 739 1293
Size: 205 534 554
Fragments arranged by size:
554 534 205
BsaI GGTCTCn'nnnn_
Cuts at: 0 193 667 979 1293
Size: 193 474 312 314
Fragments arranged by size:
474 314 312 193
BsaBI GATnn'nnATC
Cuts at: 0 964 1293
Size: 964 329
BsaWI w'CCGG_w
Cuts at: 0 42 970 1293
Size: 42 928 323
Fragments arranged by size:
928 323 42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 249 279 426 456 1293
Size: 249 30 147 30 837
Fragments arranged by size:
837 249 147 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 472 1293
Size: 472 821
BseYI C'CCAG_C
Cuts at: 0 808 1293
Size: 808 485
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 1293
Size: 709 584
BsiEI CG_ry'CG
Cuts at: 0 1155 1293
Size: 1155 138
BsiHKAI G_wGCw'C
Cuts at: 0 884 1060 1293
Size: 884 176 233
Fragments arranged by size:
884 233 176
Bsp1286I G_dGCh'C
Cuts at: 0 403 884 1060 1200 1252 1293
Size: 403 481 176 140 52 41
Fragments arranged by size:
481 403 176 140 52 41
BsrDI GCAATG_nn'
Cuts at: 0 1025 1293
Size: 1025 268
BsrFI r'CCGG_y
Cuts at: 0 42 52 970 1293
Size: 42 10 918 323
Fragments arranged by size:
918 323 42 10
BstEII G'GTnAC_C
Cuts at: 0 93 1293
Size: 93 1200
BstXI CCAn_nnnn'nTGG
Cuts at: 0 375 1293
Size: 375 918
BstYI r'GATC_y
Cuts at: 0 324 346 475 577 1293
Size: 324 22 129 102 716
Fragments arranged by size:
716 324 129 102 22
BtgI C'CryG_G
Cuts at: 0 543 1293
Size: 543 750
DraIII CAC_nnn'GTG
Cuts at: 0 1204 1293
Size: 1204 89
EaeI y'GGCC_r
Cuts at: 0 50 1265 1293
Size: 50 1215 28
Fragments arranged by size:
1215 50 28
EcoICRI GAG'CTC
Cuts at: 0 882 1293
Size: 882 411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 868 1251 1293
Size: 437 431 383 42
Fragments arranged by size:
437 431 383 42
EcoO109I rG'GnC_Cy
Cuts at: 0 65 334 714 1293
Size: 65 269 380 579
Fragments arranged by size:
579 380 269 65
EcoRV GAT'ATC
Cuts at: 0 966 1122 1293
Size: 966 156 171
Fragments arranged by size:
966 171 156
FseI GG_CCGG'CC
Cuts at: 0 56 1293
Size: 56 1237
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 739 771 1293
Size: 739 32 522
Fragments arranged by size:
739 522 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 1293
Size: 45 1248
MscI TGG'CCA
Cuts at: 0 1267 1293
Size: 1267 26
MslI CAynn'nnrTG
Cuts at: 0 229 373 595 1293
Size: 229 144 222 698
Fragments arranged by size:
698 229 222 144
MspA1I CmG'CkG
Cuts at: 0 708 1293
Size: 708 585
NaeI GCC'GGC
Cuts at: 0 54 1293
Size: 54 1239
NgoMIV G'CCGG_C
Cuts at: 0 52 1293
Size: 52 1241
NspI r_CATG'y
Cuts at: 0 174 1293
Size: 174 1119
PasI CC'CwG_GG
Cuts at: 0 329 421 573 1293
Size: 329 92 152 720
Fragments arranged by size:
720 329 152 92
PfoI T'CCnGG_A
Cuts at: 0 533 1293
Size: 533 760
PpuMI rG'GwC_Cy
Cuts at: 0 714 1293
Size: 714 579
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 139 171 994 1026 1293
Size: 139 32 823 32 267
Fragments arranged by size:
823 267 139 32 32
PstI C_TGCA'G
Cuts at: 0 713 1293
Size: 713 580
PvuI CG_AT'CG
Cuts at: 0 1155 1293
Size: 1155 138
PvuII CAG'CTG
Cuts at: 0 708 1293
Size: 708 585
SacI G_AGCT'C
Cuts at: 0 884 1293
Size: 884 409
SexAI A'CCwGG_T
Cuts at: 0 716 1293
Size: 716 577
SfcI C'TryA_G
Cuts at: 0 699 709 1293
Size: 699 10 584
Fragments arranged by size:
699 584 10
SgrAI Cr'CCGG_yG
Cuts at: 0 42 1293
Size: 42 1251
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 249 281 1293
Size: 249 32 1012
Fragments arranged by size:
1012 249 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1170 1293
Size: 1170 123
Enzymes that do cut:
AccI AgeI AlwNI ApaLI AvaI BamHI BanI BanII
BbsI BbvCI BlpI Bme1580I BplI BpmI Bpu10I BsaI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BsrDI BsrFI BstEII BstXI BstYI BtgI DraIII
EaeI EcoICRI Eco57MI EcoO109I EcoRV FseI Hin4I MmeI
MscI MslI MspA1I NaeI NgoMIV NspI PasI PfoI
PpuMI PsrI PstI PvuI PvuII SacI SexAI SfcI
SgrAI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmrI BmtI BpuEI BsaAI BsaHI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DrdI EagI EarI EciI EcoNI
EcoRI FalI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI RsrII SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TatI Tth111I XbaI XhoI XmaI XmnI
ZraI