Restriction map for
pF1KA0072
(Linear) (Six-base) MAPSORT of: KIAA0072.seq Check: 3805 from: 1 to: 1512
KIAA0072.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AccI GT'mk_AC
Cuts at: 0 947 1512
Size: 947 565
AgeI A'CCGG_T
Cuts at: 0 228 1328 1512
Size: 228 1100 184
Fragments arranged by size:
1100 228 184
AlwNI CAG_nnn'CTG
Cuts at: 0 636 1512
Size: 636 876
ApoI r'AATT_y
Cuts at: 0 336 755 1512
Size: 336 419 757
Fragments arranged by size:
757 419 336
AvaI C'yCGr_G
Cuts at: 0 1175 1512
Size: 1175 337
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 591 624 1512
Size: 591 33 888
Fragments arranged by size:
888 591 33
BamHI G'GATC_C
Cuts at: 0 967 1179 1512
Size: 967 212 333
Fragments arranged by size:
967 333 212
BanII G_rGCy'C
Cuts at: 0 1193 1281 1512
Size: 1193 88 231
Fragments arranged by size:
1193 231 88
BciVI GTATCCnnnnn_n'
Cuts at: 0 204 1512
Size: 204 1308
BglII A'GATC_T
Cuts at: 0 863 1512
Size: 863 649
BmrI ACTGGGnnnn_n'
Cuts at: 0 1054 1512
Size: 1054 458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 223 496 1207 1512
Size: 76 147 273 711 305
Fragments arranged by size:
711 305 273 147 76
Bpu10I CC'TnA_GC
Cuts at: 0 1118 1512
Size: 1118 394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 719 1512
Size: 719 793
BsaAI yAC'GTr
Cuts at: 0 237 1512
Size: 237 1275
BsaWI w'CCGG_w
Cuts at: 0 228 1328 1512
Size: 228 1100 184
Fragments arranged by size:
1100 228 184
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 1512
Size: 73 1439
BseYI C'CCAG_C
Cuts at: 0 629 1512
Size: 629 883
BsiHKAI G_wGCw'C
Cuts at: 0 1193 1512
Size: 1193 319
BsmI GAATG_Cn'
Cuts at: 0 1059 1512
Size: 1059 453
Bsp1286I G_dGCh'C
Cuts at: 0 1193 1281 1512
Size: 1193 88 231
Fragments arranged by size:
1193 231 88
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 269 1512
Size: 269 1243
BsrBI CCG'CTC
Cuts at: 0 99 144 1512
Size: 99 45 1368
Fragments arranged by size:
1368 99 45
BsrDI GCAATG_nn'
Cuts at: 0 1259 1512
Size: 1259 253
BsrFI r'CCGG_y
Cuts at: 0 228 1328 1512
Size: 228 1100 184
Fragments arranged by size:
1100 228 184
BssHII G'CGCG_C
Cuts at: 0 68 1512
Size: 68 1444
BstAPI GCAn_nnn'nTGC
Cuts at: 0 93 1512
Size: 93 1419
BstEII G'GTnAC_C
Cuts at: 0 624 1512
Size: 624 888
BstYI r'GATC_y
Cuts at: 0 863 967 1179 1512
Size: 863 104 212 333
Fragments arranged by size:
863 333 212 104
Bsu36I CC'TnA_GG
Cuts at: 0 250 1512
Size: 250 1262
BtgI C'CryG_G
Cuts at: 0 1197 1512
Size: 1197 315
BtsI GCAGTG_nn'
Cuts at: 0 98 1512
Size: 98 1414
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 915 950 1512
Size: 915 35 562
Fragments arranged by size:
915 562 35
DraI TTT'AAA
Cuts at: 0 599 1512
Size: 599 913
DraIII CAC_nnn'GTG
Cuts at: 0 81 1512
Size: 81 1431
EaeI y'GGCC_r
Cuts at: 0 1256 1512
Size: 1256 256
EarI CTCTTCn'nnn_
Cuts at: 0 151 1106 1198 1512
Size: 151 955 92 314
Fragments arranged by size:
955 314 151 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 139 1512
Size: 139 1373
EcoICRI GAG'CTC
Cuts at: 0 1191 1512
Size: 1191 321
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 223 496 1207 1512
Size: 76 147 273 711 305
Fragments arranged by size:
711 305 273 147 76
EcoO109I rG'GnC_Cy
Cuts at: 0 921 1512
Size: 921 591
HaeII r_GCGC'y
Cuts at: 0 22 52 1512
Size: 22 30 1460
Fragments arranged by size:
1460 30 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 400 432 778 810 900 932 1512
Size: 400 32 346 32 90 32 580
Fragments arranged by size:
580 400 346 90 32 32 32
HincII GTy'rAC
Cuts at: 0 258 1512
Size: 258 1254
HindIII A'AGCT_T
Cuts at: 0 1349 1512
Size: 1349 163
MfeI C'AATT_G
Cuts at: 0 1403 1512
Size: 1403 109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 936 1512
Size: 936 576
MslI CAynn'nnrTG
Cuts at: 0 284 1512
Size: 284 1228
MspA1I CmG'CkG
Cuts at: 0 54 1512
Size: 54 1458
PasI CC'CwG_GG
Cuts at: 0 1274 1512
Size: 1274 238
PflMI CCAn_nnn'nTGG
Cuts at: 0 15 222 1512
Size: 15 207 1290
Fragments arranged by size:
1290 207 15
PmlI CAC'GTG
Cuts at: 0 237 1512
Size: 237 1275
PpuMI rG'GwC_Cy
Cuts at: 0 921 1512
Size: 921 591
PstI C_TGCA'G
Cuts at: 0 89 266 1512
Size: 89 177 1246
Fragments arranged by size:
1246 177 89
SacI G_AGCT'C
Cuts at: 0 1193 1512
Size: 1193 319
SapI GCTCTTCn'nnn_
Cuts at: 0 151 1198 1512
Size: 151 1047 314
Fragments arranged by size:
1047 314 151
SfcI C'TryA_G
Cuts at: 0 85 262 419 944 1512
Size: 85 177 157 525 568
Fragments arranged by size:
568 525 177 157 85
SmlI C'TyrA_G
Cuts at: 0 734 1512
Size: 734 778
SpeI A'CTAG_T
Cuts at: 0 1374 1512
Size: 1374 138
TatI w'GTAC_w
Cuts at: 0 1106 1512
Size: 1106 406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 618 1512
Size: 618 894
XmnI GAAnn'nnTTC
Cuts at: 0 230 1512
Size: 230 1282
Enzymes that do cut:
AccI AgeI AlwNI ApoI AvaI BaeI BamHI BanII
BciVI BglII BmrI BpmI Bpu10I BpuEI BsaAI BsaWI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssHII BstAPI BstEII BstYI Bsu36I BtgI BtsI
CspCI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoO109I HaeII Hin4I HincII HindIII MfeI MmeI MslI
MspA1I PasI PflMI PmlI PpuMI PstI SacI SapI
SfcI SmlI SpeI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BanI BbeI BbsI BbvCI BcgI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI BsaI
BsaBI BsaHI BsaXI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BsrGI BssSI BstBI BstXI BstZ17I BtgZI ClaI
DrdI EagI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HpaI KasI KpnI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PfoI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI SspI StuI StyI SwaI TaqII
TstI Tth111I XbaI XhoI XmaI ZraI