Restriction map for
pF1KSDA0065
(Linear) (Six-base) MAPSORT of: KIAA0065.seq Check: 303 from: 1 to: 2430
KIAA0065.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 937 2085 2430
Size: 168 769 1148 345
Fragments arranged by size:
1148 769 345 168
AleI CACnn'nnGTG
Cuts at: 0 1388 2430
Size: 1388 1042
AlwNI CAG_nnn'CTG
Cuts at: 0 84 2430
Size: 84 2346
ApaLI G'TGCA_C
Cuts at: 0 1045 2430
Size: 1045 1385
ApoI r'AATT_y
Cuts at: 0 226 502 748 823 1463 1675 1883 2095
Size: 226 276 246 75 640 212 208 212
Cuts at: 2095 2346 2430
Size: 251 84
Fragments arranged by size:
640 276 251 246 226 212 212 208
84 75
BanII G_rGCy'C
Cuts at: 0 105 2139 2430
Size: 105 2034 291
Fragments arranged by size:
2034 291 105
BbsI GAAGACnn'nnnn_
Cuts at: 0 2310 2430
Size: 2310 120
BclI T'GATC_A
Cuts at: 0 261 2430
Size: 261 2169
BfrBI ATG'CAT
Cuts at: 0 1498 2430
Size: 1498 932
Bme1580I G_kGCm'C
Cuts at: 0 1049 2430
Size: 1049 1381
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 2430
Size: 145 2285
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 1432 2430
Size: 542 890 998
Fragments arranged by size:
998 890 542
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 291 622 2430
Size: 85 206 331 1808
Fragments arranged by size:
1808 331 206 85
BsiHKAI G_wGCw'C
Cuts at: 0 647 1049 2430
Size: 647 402 1381
Fragments arranged by size:
1381 647 402
BsmI GAATG_Cn'
Cuts at: 0 1159 2083 2430
Size: 1159 924 347
Fragments arranged by size:
1159 924 347
Bsp1286I G_dGCh'C
Cuts at: 0 105 647 1049 2139 2430
Size: 105 542 402 1090 291
Fragments arranged by size:
1090 542 402 291 105
BspHI T'CATG_A
Cuts at: 0 603 1746 2430
Size: 603 1143 684
Fragments arranged by size:
1143 684 603
BsrDI GCAATG_nn'
Cuts at: 0 1164 2088 2430
Size: 1164 924 342
Fragments arranged by size:
1164 924 342
BsrGI T'GTAC_A
Cuts at: 0 1956 2292 2430
Size: 1956 336 138
Fragments arranged by size:
1956 336 138
BtgI C'CryG_G
Cuts at: 0 208 2430
Size: 208 2222
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1146 1181 2070 2105 2430
Size: 1146 35 889 35 325
Fragments arranged by size:
1146 889 325 35 35
DraI TTT'AAA
Cuts at: 0 42 2430
Size: 42 2388
DraIII CAC_nnn'GTG
Cuts at: 0 2395 2430
Size: 2395 35
EarI CTCTTCn'nnn_
Cuts at: 0 197 703 719 2430
Size: 197 506 16 1711
Fragments arranged by size:
1711 506 197 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 168 937 2085 2430
Size: 145 23 769 1148 345
Fragments arranged by size:
1148 769 345 145 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 685 2430
Size: 685 1745
EcoRI G'AATT_C
Cuts at: 0 226 1463 1883 2430
Size: 226 1237 420 547
Fragments arranged by size:
1237 547 420 226
EcoRV GAT'ATC
Cuts at: 0 797 2430
Size: 797 1633
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 19 51 246 278 760 792 2430
Size: 19 32 195 32 482 32 1638
Fragments arranged by size:
1638 482 195 32 32 32 19
HincII GTy'rAC
Cuts at: 0 1694 2430
Size: 1694 736
HindIII A'AGCT_T
Cuts at: 0 240 1004 1088 1928 2430
Size: 240 764 84 840 502
Fragments arranged by size:
840 764 502 240 84
MslI CAynn'nnrTG
Cuts at: 0 1388 1751 2430
Size: 1388 363 679
Fragments arranged by size:
1388 679 363
MspA1I CmG'CkG
Cuts at: 0 259 2430
Size: 259 2171
NcoI C'CATG_G
Cuts at: 0 208 2430
Size: 208 2222
NsiI A_TGCA'T
Cuts at: 0 1500 2430
Size: 1500 930
NspI r_CATG'y
Cuts at: 0 1502 1661 2258 2430
Size: 1502 159 597 172
Fragments arranged by size:
1502 597 172 159
PshAI GACnn'nnGTC
Cuts at: 0 92 2430
Size: 92 2338
PsiI TTA'TAA
Cuts at: 0 743 2430
Size: 743 1687
PvuII CAG'CTG
Cuts at: 0 259 2430
Size: 259 2171
SapI GCTCTTCn'nnn_
Cuts at: 0 197 2430
Size: 197 2233
SfcI C'TryA_G
Cuts at: 0 132 2430
Size: 132 2298
SmlI C'TyrA_G
Cuts at: 0 557 1411 2430
Size: 557 854 1019
Fragments arranged by size:
1019 854 557
SspI AAT'ATT
Cuts at: 0 534 2430
Size: 534 1896
StyI C'CwwG_G
Cuts at: 0 208 2430
Size: 208 2222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 79 2430
Size: 79 2351
TatI w'GTAC_w
Cuts at: 0 1368 1620 1956 2124 2292 2430
Size: 1368 252 336 168 168 138
Fragments arranged by size:
1368 336 252 168 168 138
XmnI GAAnn'nnTTC
Cuts at: 0 922 1006 1510 1594 2098 2266 2430
Size: 922 84 504 84 504 168 164
Fragments arranged by size:
922 504 504 168 164 84 84
Enzymes that do cut:
AcuI AleI AlwNI ApaLI ApoI BanII BbsI BclI
BfrBI Bme1580I BpmI BpuEI BseRI BsiHKAI BsmI Bsp1286I
BspHI BsrDI BsrGI BtgI CspCI DraI DraIII EarI
Eco57MI EcoNI EcoRI EcoRV Hin4I HincII HindIII MslI
MspA1I NcoI NsiI NspI PshAI PsiI PvuII SapI
SfcI SmlI SspI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BglI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI DrdI EaeI EagI EciI
EcoICRI EcoO109I FalI FseI FspI FspAI HaeII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI