Restriction map for
pF1KA0058
(Linear) (Six-base) MAPSORT of: KIAA0058.seq Check: 2196 from: 1 to: 504
KIAA0058.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 126 325 504
Size: 126 199 179
Fragments arranged by size:
199 179 126
AccI GT'mk_AC
Cuts at: 0 65 504
Size: 65 439
AlwNI CAG_nnn'CTG
Cuts at: 0 163 504
Size: 163 341
ApaLI G'TGCA_C
Cuts at: 0 151 504
Size: 151 353
BanI G'GyrC_C
Cuts at: 0 337 504
Size: 337 167
BanII G_rGCy'C
Cuts at: 0 134 504
Size: 134 370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 132 166 504
Size: 132 34 338
Fragments arranged by size:
338 132 34
BlpI GC'TnA_GC
Cuts at: 0 410 504
Size: 410 94
Bme1580I G_kGCm'C
Cuts at: 0 155 504
Size: 155 349
BmrI ACTGGGnnnn_n'
Cuts at: 0 219 504
Size: 219 285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 504
Size: 218 286
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 96 126 422 452 504
Size: 96 30 296 30 52
Fragments arranged by size:
296 96 52 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 368 504
Size: 368 136
BseYI C'CCAG_C
Cuts at: 0 352 504
Size: 352 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 504
Size: 64 440
BsiHKAI G_wGCw'C
Cuts at: 0 134 155 504
Size: 134 21 349
Fragments arranged by size:
349 134 21
Bsp1286I G_dGCh'C
Cuts at: 0 134 155 504
Size: 134 21 349
Fragments arranged by size:
349 134 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 126 325 504
Size: 126 199 179
Fragments arranged by size:
199 179 126
BstXI CCAn_nnnn'nTGG
Cuts at: 0 312 504
Size: 312 192
BstZ17I GTA'TAC
Cuts at: 0 66 504
Size: 66 438
Bsu36I CC'TnA_GG
Cuts at: 0 89 504
Size: 89 415
BtgI C'CryG_G
Cuts at: 0 218 263 504
Size: 218 45 241
Fragments arranged by size:
241 218 45
EcoICRI GAG'CTC
Cuts at: 0 132 504
Size: 132 372
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 504
Size: 218 286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 480 504
Size: 480 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 66 98 422 454 504
Size: 66 32 324 32 50
Fragments arranged by size:
324 66 50 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 504
Size: 46 458
MspA1I CmG'CkG
Cuts at: 0 453 504
Size: 453 51
NcoI C'CATG_G
Cuts at: 0 218 263 504
Size: 218 45 241
Fragments arranged by size:
241 218 45
PasI CC'CwG_GG
Cuts at: 0 157 504
Size: 157 347
PflMI CCAn_nnn'nTGG
Cuts at: 0 231 388 504
Size: 231 157 116
Fragments arranged by size:
231 157 116
PfoI T'CCnGG_A
Cuts at: 0 195 387 504
Size: 195 192 117
Fragments arranged by size:
195 192 117
SacI G_AGCT'C
Cuts at: 0 134 504
Size: 134 370
StyI C'CwwG_G
Cuts at: 0 218 263 504
Size: 218 45 241
Fragments arranged by size:
241 218 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 170 271 463 504
Size: 170 101 192 41
Fragments arranged by size:
192 170 101 41
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 261 312 504
Size: 261 51 192
Fragments arranged by size:
261 192 51
XmnI GAAnn'nnTTC
Cuts at: 0 466 504
Size: 466 38
Enzymes that do cut:
AarI AccI AlwNI ApaLI BanI BanII BcgI BlpI
Bme1580I BmrI BpmI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BstXI BstZ17I Bsu36I BtgI EcoICRI Eco57MI
FalI Hin4I MmeI MspA1I NcoI PasI PflMI PfoI
SacI StyI TaqII XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BmgBI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI MslI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XhoI XmaI ZraI