Restriction map for
pF1KSDA0043
(Linear) (Six-base) MAPSORT of: KIAA0043.seq Check: 1640 from: 1 to: 2178
KIAA0043.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 941 2178
Size: 941 1237
AatII G_ACGT'C
Cuts at: 0 760 2178
Size: 760 1418
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1429 1669 2178
Size: 1429 240 509
Fragments arranged by size:
1429 509 240
AflIII A'CryG_T
Cuts at: 0 1217 2178
Size: 1217 961
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1688 2178
Size: 1688 490
ApaI G_GGCC'C
Cuts at: 0 52 2095 2178
Size: 52 2043 83
Fragments arranged by size:
2043 83 52
ApaLI G'TGCA_C
Cuts at: 0 1417 1786 2178
Size: 1417 369 392
Fragments arranged by size:
1417 392 369
ApoI r'AATT_y
Cuts at: 0 392 2178
Size: 392 1786
AvaI C'yCGr_G
Cuts at: 0 45 1762 1802 2178
Size: 45 1717 40 376
Fragments arranged by size:
1717 376 45 40
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 584 617 2178
Size: 584 33 1561
Fragments arranged by size:
1561 584 33
BamHI G'GATC_C
Cuts at: 0 32 2178
Size: 32 2146
BanI G'GyrC_C
Cuts at: 0 891 1263 2178
Size: 891 372 915
Fragments arranged by size:
915 891 372
BanII G_rGCy'C
Cuts at: 0 52 489 1256 1339 1811 2095 2178
Size: 52 437 767 83 472 284 83
Fragments arranged by size:
767 472 437 284 83 83 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 148 2178
Size: 148 2030
BbvCI CC'TCA_GC
Cuts at: 0 1072 2178
Size: 1072 1106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1946 1980 2178
Size: 1946 34 198
Fragments arranged by size:
1946 198 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1285 1567 1585 1615 2178
Size: 1285 282 18 30 563
Fragments arranged by size:
1285 563 282 30 18
BlpI GC'TnA_GC
Cuts at: 0 1589 1738 2035 2076 2104 2178
Size: 1589 149 297 41 28 74
Fragments arranged by size:
1589 297 149 74 41 28
Bme1580I G_kGCm'C
Cuts at: 0 52 560 896 1421 1790 2095 2178
Size: 52 508 336 525 369 305 83
Fragments arranged by size:
525 508 369 336 305 83 52
BmgBI CAC'GTC
Cuts at: 0 626 1218 2178
Size: 626 592 960
Fragments arranged by size:
960 626 592
BmrI ACTGGGnnnn_n'
Cuts at: 0 628 1444 2178
Size: 628 816 734
Fragments arranged by size:
816 734 628
BmtI G_CTAG'C
Cuts at: 0 1981 2178
Size: 1981 197
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1353 2178
Size: 1353 825
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1048 1195 1906 2127 2178
Size: 898 150 147 711 221 51
Fragments arranged by size:
898 711 221 150 147 51
Bpu10I CC'TnA_GC
Cuts at: 0 1072 1816 1982 2178
Size: 1072 744 166 196
Fragments arranged by size:
1072 744 196 166
BsaI GGTCTCn'nnnn_
Cuts at: 0 81 2178
Size: 81 2097
BsaHI Gr'CG_yC
Cuts at: 0 577 757 2178
Size: 577 180 1421
Fragments arranged by size:
1421 577 180
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 732 762 2178
Size: 732 30 1416
Fragments arranged by size:
1416 732 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 650 1345 1381 1384 1711 1714 1987 2109
Size: 650 695 36 3 327 3 273 122
Cuts at: 2109 2178
Size: 69
Fragments arranged by size:
695 650 327 273 122 69 36 3
3
BseYI C'CCAG_C
Cuts at: 0 85 534 642 896 1773 2178
Size: 85 449 108 254 877 405
Fragments arranged by size:
877 449 405 254 108 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1018 2178
Size: 1018 1160
BsiEI CG_ry'CG
Cuts at: 0 99 518 1637 2178
Size: 99 419 1119 541
Fragments arranged by size:
1119 541 419 99
BsiHKAI G_wGCw'C
Cuts at: 0 932 1339 1421 1790 2178
Size: 932 407 82 369 388
Fragments arranged by size:
932 407 388 369 82
BsmBI CGTCTCn'nnnn_
Cuts at: 0 573 2178
Size: 573 1605
Bsp1286I G_dGCh'C
Cuts at: 0 52 489 560 896 932 1256 1339 1421
Size: 52 437 71 336 36 324 83 82
Cuts at: 1421 1790 1811 2095 2178
Size: 369 21 284 83
Fragments arranged by size:
437 369 336 324 284 83 83 82
71 52 36 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 215 487 941 2178
Size: 215 272 454 1237
Fragments arranged by size:
1237 454 272 215
BsrBI CCG'CTC
Cuts at: 0 1374 2178
Size: 1374 804
BsrFI r'CCGG_y
Cuts at: 0 474 1266 1636 1754 2178
Size: 474 792 370 118 424
Fragments arranged by size:
792 474 424 370 118
BssSI C'ACGA_G
Cuts at: 0 1183 1420 2178
Size: 1183 237 758
Fragments arranged by size:
1183 758 237
BstAPI GCAn_nnn'nTGC
Cuts at: 0 123 2178
Size: 123 2055
BstXI CCAn_nnnn'nTGG
Cuts at: 0 825 1386 2178
Size: 825 561 792
Fragments arranged by size:
825 792 561
BstYI r'GATC_y
Cuts at: 0 32 2178
Size: 32 2146
Bsu36I CC'TnA_GG
Cuts at: 0 955 2085 2178
Size: 955 1130 93
Fragments arranged by size:
1130 955 93
BtgI C'CryG_G
Cuts at: 0 26 185 683 1196 1256 1283 1292 1631
Size: 26 159 498 513 60 27 9 339
Cuts at: 1631 2178
Size: 547
Fragments arranged by size:
547 513 498 339 159 60 27 26
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 570 2178
Size: 570 1608
BtsI GCAGTG_nn'
Cuts at: 0 611 767 1332 2172 2178
Size: 611 156 565 840 6
Fragments arranged by size:
840 611 565 156 6
DraIII CAC_nnn'GTG
Cuts at: 0 1536 2178
Size: 1536 642
EaeI y'GGCC_r
Cuts at: 0 96 515 761 846 1098 1193 1430 1634
Size: 96 419 246 85 252 95 237 204
Cuts at: 1634 2178
Size: 544
Fragments arranged by size:
544 419 252 246 237 204 96 95
85
EagI C'GGCC_G
Cuts at: 0 96 515 1634 2178
Size: 96 419 1119 544
Fragments arranged by size:
1119 544 419 96
EarI CTCTTCn'nnn_
Cuts at: 0 256 1344 1544 1688 2178
Size: 256 1088 200 144 490
Fragments arranged by size:
1088 490 256 200 144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1405 1562 2178
Size: 1405 157 616
Fragments arranged by size:
1405 616 157
EcoICRI GAG'CTC
Cuts at: 0 1337 2178
Size: 1337 841
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1048 1195 1429 1669 1906 2127 2178
Size: 898 150 147 234 240 237 221 51
Fragments arranged by size:
898 240 237 234 221 150 147 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1441 1815 2178
Size: 1441 374 363
Fragments arranged by size:
1441 374 363
EcoO109I rG'GnC_Cy
Cuts at: 0 49 873 1023 1446 1704 2091 2178
Size: 49 824 150 423 258 387 87
Fragments arranged by size:
824 423 387 258 150 87 49
HaeII r_GCGC'y
Cuts at: 0 1273 1310 1734 2178
Size: 1273 37 424 444
Fragments arranged by size:
1273 444 424 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 952 984 2178
Size: 952 32 1194
Fragments arranged by size:
1194 952 32
HindIII A'AGCT_T
Cuts at: 0 380 2178
Size: 380 1798
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 448 905 1850 2178
Size: 104 344 457 945 328
Fragments arranged by size:
945 457 344 328 104
MscI TGG'CCA
Cuts at: 0 1195 2178
Size: 1195 983
MspA1I CmG'CkG
Cuts at: 0 28 114 646 1285 1407 1428 1647 2027
Size: 28 86 532 639 122 21 219 380
Cuts at: 2027 2034 2147 2178
Size: 7 113 31
Fragments arranged by size:
639 532 380 219 122 113 86 31
28 21 7
NaeI GCC'GGC
Cuts at: 0 476 1638 2178
Size: 476 1162 540
Fragments arranged by size:
1162 540 476
NcoI C'CATG_G
Cuts at: 0 1196 1292 2178
Size: 1196 96 886
Fragments arranged by size:
1196 886 96
NgoMIV G'CCGG_C
Cuts at: 0 474 1636 2178
Size: 474 1162 542
Fragments arranged by size:
1162 542 474
NheI G'CTAG_C
Cuts at: 0 1977 2178
Size: 1977 201
NspI r_CATG'y
Cuts at: 0 126 2178
Size: 126 2052
PflMI CCAn_nnn'nTGG
Cuts at: 0 186 2178
Size: 186 1992
PfoI T'CCnGG_A
Cuts at: 0 1211 2178
Size: 1211 967
PpuMI rG'GwC_Cy
Cuts at: 0 873 2178
Size: 873 1305
PshAI GACnn'nnGTC
Cuts at: 0 580 2178
Size: 580 1598
PsiI TTA'TAA
Cuts at: 0 344 2178
Size: 344 1834
PspOMI G'GGCC_C
Cuts at: 0 48 2091 2178
Size: 48 2043 87
Fragments arranged by size:
2043 87 48
PstI C_TGCA'G
Cuts at: 0 119 1400 2018 2178
Size: 119 1281 618 160
Fragments arranged by size:
1281 618 160 119
PvuII CAG'CTG
Cuts at: 0 114 646 1407 1428 1647 2034 2178
Size: 114 532 761 21 219 387 144
Fragments arranged by size:
761 532 387 219 144 114 21
SacI G_AGCT'C
Cuts at: 0 1339 2178
Size: 1339 839
SacII CC_GC'GG
Cuts at: 0 29 1286 2178
Size: 29 1257 892
Fragments arranged by size:
1257 892 29
SapI GCTCTTCn'nnn_
Cuts at: 0 1344 2178
Size: 1344 834
SfcI C'TryA_G
Cuts at: 0 115 1396 2014 2178
Size: 115 1281 618 164
Fragments arranged by size:
1281 618 164 115
SgrAI Cr'CCGG_yG
Cuts at: 0 1266 2178
Size: 1266 912
SmaI CCC'GGG
Cuts at: 0 47 1764 2178
Size: 47 1717 414
Fragments arranged by size:
1717 414 47
StyI C'CwwG_G
Cuts at: 0 1196 1292 1562 2178
Size: 1196 96 270 616
Fragments arranged by size:
1196 616 270 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 618 687 2178
Size: 618 69 1491
Fragments arranged by size:
1491 618 69
TatI w'GTAC_w
Cuts at: 0 119 2178
Size: 119 2059
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 662 694 732 764 2178
Size: 662 32 38 32 1414
Fragments arranged by size:
1414 662 38 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 681 873 2178
Size: 681 192 1305
Fragments arranged by size:
1305 681 192
XmaI C'CCGG_G
Cuts at: 0 45 1762 2178
Size: 45 1717 416
Fragments arranged by size:
1717 416 45
XmnI GAAnn'nnTTC
Cuts at: 0 1930 2178
Size: 1930 248
ZraI GAC'GTC
Cuts at: 0 758 2178
Size: 758 1420
Enzymes that do cut:
AarI AatII AcuI AflIII AlfI ApaI ApaLI ApoI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I HindIII MmeI MscI MspA1I NaeI NcoI NgoMIV
NheI NspI PflMI PfoI PpuMI PshAI PsiI PspOMI
PstI PvuII SacI SacII SapI SfcI SgrAI SmaI
StyI TaqII TatI TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI AlwNI AscI AseI AsiSI AvrII BbeI BciVI
BclI BfrBI BglII BpuEI BsaAI BsaBI BsaWI BsiWI
BsmI BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII
BstZ17I ClaI CspCI DraI DrdI EcoRI EcoRV FalI
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI MslI NarI NdeI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PspXI PsrI PvuI
RsrII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XhoI