Restriction map for
pF1KSDA0037
(Linear) (Six-base) MAPSORT of: KIAA0037.seq Check: 3059 from: 1 to: 3240
KIAA0037.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 363 1068 2176 3240
Size: 363 705 1108 1064
Fragments arranged by size:
1108 1064 705 363
Acc65I G'GTAC_C
Cuts at: 0 1457 2609 3240
Size: 1457 1152 631
Fragments arranged by size:
1457 1152 631
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 408 1447 2404 2779 3240
Size: 144 264 1039 957 375 461
Fragments arranged by size:
1039 957 461 375 264 144
AflIII A'CryG_T
Cuts at: 0 1058 1178 1193 3240
Size: 1058 120 15 2047
Fragments arranged by size:
2047 1058 120 15
AhdI GACnn_n'nnGTC
Cuts at: 0 1182 3240
Size: 1182 2058
AleI CACnn'nnGTG
Cuts at: 0 1096 2882 3240
Size: 1096 1786 358
Fragments arranged by size:
1786 1096 358
AlwNI CAG_nnn'CTG
Cuts at: 0 540 881 1995 2859 3228 3240
Size: 540 341 1114 864 369 12
Fragments arranged by size:
1114 864 540 369 341 12
ApaI G_GGCC'C
Cuts at: 0 46 1779 3012 3240
Size: 46 1733 1233 228
Fragments arranged by size:
1733 1233 228 46
ApaLI G'TGCA_C
Cuts at: 0 1240 3240
Size: 1240 2000
AvaI C'yCGr_G
Cuts at: 0 614 1128 1462 1772 1932 1949 3130 3240
Size: 614 514 334 310 160 17 1181 110
Fragments arranged by size:
1181 614 514 334 310 160 110 17
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3126 3159 3240
Size: 3126 33 81
Fragments arranged by size:
3126 81 33
BanI G'GyrC_C
Cuts at: 0 375 580 821 1457 1761 2344 2356 2609
Size: 375 205 241 636 304 583 12 253
Cuts at: 2609 3240
Size: 631
Fragments arranged by size:
636 631 583 375 304 253 241 205
12
BanII G_rGCy'C
Cuts at: 0 46 920 1667 1779 2142 2153 2753 2828
Size: 46 874 747 112 363 11 600 75
Cuts at: 2828 3012 3240
Size: 184 228
Fragments arranged by size:
874 747 600 363 228 184 112 75
46 11
BbeI G_GCGC'C
Cuts at: 0 584 3240
Size: 584 2656
BbsI GAAGACnn'nnnn_
Cuts at: 0 390 2196 2392 3240
Size: 390 1806 196 848
Fragments arranged by size:
1806 848 390 196
BbvCI CC'TCA_GC
Cuts at: 0 1999 3184 3240
Size: 1999 1185 56
Fragments arranged by size:
1999 1185 56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1806 1840 3240
Size: 1806 34 1400
Fragments arranged by size:
1806 1400 34
BclI T'GATC_A
Cuts at: 0 3158 3240
Size: 3158 82
BglI GCCn_nnn'nGGC
Cuts at: 0 139 451 2257 2569 3240
Size: 139 312 1806 312 671
Fragments arranged by size:
1806 671 312 312 139
BlpI GC'TnA_GC
Cuts at: 0 1666 2827 3240
Size: 1666 1161 413
Fragments arranged by size:
1666 1161 413
Bme1580I G_kGCm'C
Cuts at: 0 46 380 1244 1301 1779 2846 3012 3240
Size: 46 334 864 57 478 1067 166 228
Fragments arranged by size:
1067 864 478 334 228 166 57 46
BmgBI CAC'GTC
Cuts at: 0 1179 1194 1536 3240
Size: 1179 15 342 1704
Fragments arranged by size:
1704 1179 342 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 509 1870 1903 3240
Size: 509 1361 33 1337
Fragments arranged by size:
1361 1337 509 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1644 3240
Size: 1644 1596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 1750 2242 2424 2566 2725 2881 3017
Size: 162 1588 492 182 142 159 156 136
Cuts at: 3017 3095 3106 3240
Size: 78 11 134
Fragments arranged by size:
1588 492 182 162 159 156 142 136
134 78 11
Bpu10I CC'TnA_GC
Cuts at: 0 277 1999 3184 3240
Size: 277 1722 1185 56
Fragments arranged by size:
1722 1185 277 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 1309 3240
Size: 742 567 1931
Fragments arranged by size:
1931 742 567
BsaI GGTCTCn'nnnn_
Cuts at: 0 1522 2509 3100 3240
Size: 1522 987 591 140
Fragments arranged by size:
1522 987 591 140
BsaAI yAC'GTr
Cuts at: 0 2568 2807 3240
Size: 2568 239 433
Fragments arranged by size:
2568 433 239
BsaBI GATnn'nnATC
Cuts at: 0 1336 3240
Size: 1336 1904
BsaHI Gr'CG_yC
Cuts at: 0 581 1438 3240
Size: 581 857 1802
Fragments arranged by size:
1802 857 581
BsaWI w'CCGG_w
Cuts at: 0 1208 3240
Size: 1208 2032
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2926 2956 3240
Size: 2926 30 284
Fragments arranged by size:
2926 284 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 2521 3118 3240
Size: 52 2469 597 122
Fragments arranged by size:
2469 597 122 52
BseYI C'CCAG_C
Cuts at: 0 318 1387 1568 1905 1944 3228 3240
Size: 318 1069 181 337 39 1284 12
Fragments arranged by size:
1284 1069 337 318 181 39 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1947 3096 3240
Size: 1947 1149 144
Fragments arranged by size:
1947 1149 144
BsiEI CG_ry'CG
Cuts at: 0 1236 1618 3240
Size: 1236 382 1622
Fragments arranged by size:
1622 1236 382
BsiHKAI G_wGCw'C
Cuts at: 0 485 920 1244 2273 2504 2753 3240
Size: 485 435 324 1029 231 249 487
Fragments arranged by size:
1029 487 485 435 324 249 231
BsiWI C'GTAC_G
Cuts at: 0 1280 1631 3240
Size: 1280 351 1609
Fragments arranged by size:
1609 1280 351
BsmI GAATG_Cn'
Cuts at: 0 3151 3240
Size: 3151 89
BsmBI CGTCTCn'nnnn_
Cuts at: 0 462 3240
Size: 462 2778
Bsp1286I G_dGCh'C
Cuts at: 0 46 380 485 920 1244 1301 1667 1779
Size: 46 334 105 435 324 57 366 112
Cuts at: 1779 2142 2153 2273 2504 2753 2828 2846 3012
Size: 363 11 120 231 249 75 18 166
Cuts at: 3012 3240
Size: 228
Fragments arranged by size:
435 366 363 334 324 249 231 228
166 120 112 105 75 57 46 18
11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 363 701 1068 2066 2176 3118 3240
Size: 363 338 367 998 110 942 122
Fragments arranged by size:
998 942 367 363 338 122 110
BsrBI CCG'CTC
Cuts at: 0 2865 3240
Size: 2865 375
BsrDI GCAATG_nn'
Cuts at: 0 152 2106 2732 3240
Size: 152 1954 626 508
Fragments arranged by size:
1954 626 508 152
BsrGI T'GTAC_A
Cuts at: 0 407 3240
Size: 407 2833
BssSI C'ACGA_G
Cuts at: 0 1674 2503 2845 3240
Size: 1674 829 342 395
Fragments arranged by size:
1674 829 395 342
BstAPI GCAn_nnn'nTGC
Cuts at: 0 600 3240
Size: 600 2640
BstEII G'GTnAC_C
Cuts at: 0 769 3096 3240
Size: 769 2327 144
Fragments arranged by size:
2327 769 144
BstXI CCAn_nnnn'nTGG
Cuts at: 0 897 1098 1212 1728 3063 3240
Size: 897 201 114 516 1335 177
Fragments arranged by size:
1335 897 516 201 177 114
BstYI r'GATC_y
Cuts at: 0 650 971 3240
Size: 650 321 2269
Fragments arranged by size:
2269 650 321
Bsu36I CC'TnA_GG
Cuts at: 0 2209 2838 3240
Size: 2209 629 402
Fragments arranged by size:
2209 629 402
BtgI C'CryG_G
Cuts at: 0 725 1091 2519 2979 3150 3240
Size: 725 366 1428 460 171 90
Fragments arranged by size:
1428 725 460 366 171 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2175 2699 2889 3240
Size: 2175 524 190 351
Fragments arranged by size:
2175 524 351 190
BtsI GCAGTG_nn'
Cuts at: 0 128 461 1527 2302 3209 3240
Size: 128 333 1066 775 907 31
Fragments arranged by size:
1066 907 775 333 128 31
DraIII CAC_nnn'GTG
Cuts at: 0 517 1194 1864 2681 3240
Size: 517 677 670 817 559
Fragments arranged by size:
817 677 670 559 517
EaeI y'GGCC_r
Cuts at: 0 122 542 740 872 1202 1233 2012 2045
Size: 122 420 198 132 330 31 779 33
Cuts at: 2045 2117 2310 3053 3240
Size: 72 193 743 187
Fragments arranged by size:
779 743 420 330 198 193 187 132
122 72 33 31
EagI C'GGCC_G
Cuts at: 0 1233 3240
Size: 1233 2007
EarI CTCTTCn'nnn_
Cuts at: 0 628 2278 3240
Size: 628 1650 962
Fragments arranged by size:
1650 962 628
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 643 1563 2947 3240
Size: 643 920 1384 293
Fragments arranged by size:
1384 920 643 293
EcoICRI GAG'CTC
Cuts at: 0 918 2751 3240
Size: 918 1833 489
Fragments arranged by size:
1833 918 489
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 162 408 1447 1750 2242 2404 2424
Size: 144 18 246 1039 303 492 162 20
Cuts at: 2424 2566 2725 2779 2881 3017 3095 3106 3240
Size: 142 159 54 102 136 78 11 134
Fragments arranged by size:
1039 492 303 246 162 159 144 142
136 134 102 78 54 20 18 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 28 1273 1732 3240
Size: 28 1245 459 1508
Fragments arranged by size:
1508 1245 459 28
EcoO109I rG'GnC_Cy
Cuts at: 0 42 43 171 281 618 1314 1679 2379
Size: 42 1 128 110 337 696 365 700
Cuts at: 2379 3008 3126 3240
Size: 629 118 114
Fragments arranged by size:
700 696 629 365 337 128 118 114
110 42 1
FspI TGC'GCA
Cuts at: 0 667 1162 1447 3240
Size: 667 495 285 1793
Fragments arranged by size:
1793 667 495 285
FspAI rTGC'GCAy
Cuts at: 0 1447 3240
Size: 1447 1793
HaeII r_GCGC'y
Cuts at: 0 18 273 292 319 440 584 681 1575
Size: 18 255 19 27 121 144 97 894
Cuts at: 1575 3240
Size: 1665
Fragments arranged by size:
1665 894 255 144 121 97 27 19
18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1168 1200 1633 1665 3240
Size: 1168 32 433 32 1575
Fragments arranged by size:
1575 1168 433 32 32
HincII GTy'rAC
Cuts at: 0 2691 3240
Size: 2691 549
KasI G'GCGC_C
Cuts at: 0 580 3240
Size: 580 2660
KpnI G_GTAC'C
Cuts at: 0 1461 2613 3240
Size: 1461 1152 627
Fragments arranged by size:
1461 1152 627
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1598 3240
Size: 1598 1642
MscI TGG'CCA
Cuts at: 0 544 742 874 1204 2047 3055 3240
Size: 544 198 132 330 843 1008 185
Fragments arranged by size:
1008 843 544 330 198 185 132
MslI CAynn'nnrTG
Cuts at: 0 724 730 827 1096 1108 1114 2809 2882
Size: 724 6 97 269 12 6 1695 73
Cuts at: 2882 3240
Size: 358
Fragments arranged by size:
1695 724 358 269 97 73 12 6
6
MspA1I CmG'CkG
Cuts at: 0 99 537 870 1311 2174 2774 2818 3240
Size: 99 438 333 441 863 600 44 422
Fragments arranged by size:
863 600 441 438 422 333 99 44
NarI GG'CG_CC
Cuts at: 0 581 3240
Size: 581 2659
NcoI C'CATG_G
Cuts at: 0 725 2519 2979 3240
Size: 725 1794 460 261
Fragments arranged by size:
1794 725 460 261
NspI r_CATG'y
Cuts at: 0 432 786 963 1062 1113 1602 2875 3240
Size: 432 354 177 99 51 489 1273 365
Fragments arranged by size:
1273 489 432 365 354 177 99 51
PciI A'CATG_T
Cuts at: 0 1058 3240
Size: 1058 2182
PflMI CCAn_nnn'nTGG
Cuts at: 0 477 2319 2859 3240
Size: 477 1842 540 381
Fragments arranged by size:
1842 540 477 381
PfoI T'CCnGG_A
Cuts at: 0 1727 2371 3240
Size: 1727 644 869
Fragments arranged by size:
1727 869 644
PmlI CAC'GTG
Cuts at: 0 2568 3240
Size: 2568 672
PpuMI rG'GwC_Cy
Cuts at: 0 171 618 1314 2379 3240
Size: 171 447 696 1065 861
Fragments arranged by size:
1065 861 696 447 171
PshAI GACnn'nnGTC
Cuts at: 0 1534 3240
Size: 1534 1706
PspOMI G'GGCC_C
Cuts at: 0 42 1775 3008 3240
Size: 42 1733 1233 232
Fragments arranged by size:
1733 1233 232 42
PspXI vC'TCGA_Gb
Cuts at: 0 1462 3240
Size: 1462 1778
PstI C_TGCA'G
Cuts at: 0 536 2173 2823 3240
Size: 536 1637 650 417
Fragments arranged by size:
1637 650 536 417
PvuII CAG'CTG
Cuts at: 0 537 870 2174 2818 3240
Size: 537 333 1304 644 422
Fragments arranged by size:
1304 644 537 422 333
SacI G_AGCT'C
Cuts at: 0 920 2753 3240
Size: 920 1833 487
Fragments arranged by size:
1833 920 487
SanDI GG'GwC_CC
Cuts at: 0 171 1314 3240
Size: 171 1143 1926
Fragments arranged by size:
1926 1143 171
SapI GCTCTTCn'nnn_
Cuts at: 0 2278 3240
Size: 2278 962
SexAI A'CCwGG_T
Cuts at: 0 1349 2264 2405 3240
Size: 1349 915 141 835
Fragments arranged by size:
1349 915 835 141
SfcI C'TryA_G
Cuts at: 0 532 2169 2819 3240
Size: 532 1637 650 421
Fragments arranged by size:
1637 650 532 421
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2257 3240
Size: 2257 983
SfoI GGC'GCC
Cuts at: 0 582 3240
Size: 582 2658
SmaI CCC'GGG
Cuts at: 0 616 1774 3240
Size: 616 1158 1466
Fragments arranged by size:
1466 1158 616
SmlI C'TyrA_G
Cuts at: 0 757 1324 1462 1932 3240
Size: 757 567 138 470 1308
Fragments arranged by size:
1308 757 567 470 138
SphI G_CATG'C
Cuts at: 0 432 786 963 2875 3240
Size: 432 354 177 1912 365
Fragments arranged by size:
1912 432 365 354 177
StuI AGG'CCT
Cuts at: 0 1554 2111 2318 3240
Size: 1554 557 207 922
Fragments arranged by size:
1554 922 557 207
StyI C'CwwG_G
Cuts at: 0 8 284 725 890 944 1409 1607 2313
Size: 8 276 441 165 54 465 198 706
Cuts at: 2313 2519 2979 3240
Size: 206 460 261
Fragments arranged by size:
706 465 460 441 276 261 206 198
165 54 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 528 1779 1852 2190 3240
Size: 528 1251 73 338 1050
Fragments arranged by size:
1251 1050 528 338 73
TatI w'GTAC_w
Cuts at: 0 407 1890 3240
Size: 407 1483 1350
Fragments arranged by size:
1483 1350 407
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 503 535 1180 1212 3240
Size: 503 32 645 32 2028
Fragments arranged by size:
2028 645 503 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2629 3240
Size: 2629 611
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 135 198 522 897 2294 2403 3240
Size: 135 63 324 375 1397 109 837
Fragments arranged by size:
1397 837 375 324 135 109 63
XhoI C'TCGA_G
Cuts at: 0 1462 1932 3240
Size: 1462 470 1308
Fragments arranged by size:
1462 1308 470
XmaI C'CCGG_G
Cuts at: 0 614 1772 3240
Size: 614 1158 1468
Fragments arranged by size:
1468 1158 614
XmnI GAAnn'nnTTC
Cuts at: 0 2488 3240
Size: 2488 752
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI AleI AlwNI ApaI
ApaLI AvaI BaeI BanI BanII BbeI BbsI BbvCI
BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrGI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FspI FspAI HaeII Hin4I HincII KasI KpnI MmeI
MscI MslI MspA1I NarI NcoI NspI PciI PflMI
PfoI PmlI PpuMI PshAI PspOMI PspXI PstI PvuII
SacI SanDI SapI SexAI SfcI SfiI SfoI SmaI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AlfI AloI
ApoI AscI AseI AsiSI AvrII BamHI BciVI BfrBI
BglII BmtI BspEI BspHI BsrFI BssHII BstBI BstZ17I
ClaI CspCI DraI DrdI EcoRI EcoRV FalI FseI
HindIII HpaI MfeI MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PpiI PsiI
PsrI PvuI RsrII SacII SalI SbfI ScaI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI XbaI ZraI