Restriction map for
pF1KSDA0032
(Linear) (Six-base) MAPSORT of: KIAA0032.seq Check: 3855 from: 1 to: 3150
KIAA0032.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2013 3150
Size: 2013 1137
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1152 2519 2765 3150
Size: 1152 1367 246 385
Fragments arranged by size:
1367 1152 385 246
AflIII A'CryG_T
Cuts at: 0 169 2336 2338 3150
Size: 169 2167 2 812
Fragments arranged by size:
2167 812 169 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 67 3150
Size: 67 3083
AlwNI CAG_nnn'CTG
Cuts at: 0 628 2008 3150
Size: 628 1380 1142
Fragments arranged by size:
1380 1142 628
ApoI r'AATT_y
Cuts at: 0 2245 2662 3150
Size: 2245 417 488
Fragments arranged by size:
2245 488 417
AvaI C'yCGr_G
Cuts at: 0 1061 2533 3150
Size: 1061 1472 617
Fragments arranged by size:
1472 1061 617
BamHI G'GATC_C
Cuts at: 0 566 3150
Size: 566 2584
BanII G_rGCy'C
Cuts at: 0 74 240 600 671 2771 3150
Size: 74 166 360 71 2100 379
Fragments arranged by size:
2100 379 360 166 74 71
BbsI GAAGACnn'nnnn_
Cuts at: 0 1880 2049 2480 2682 3150
Size: 1880 169 431 202 468
Fragments arranged by size:
1880 468 431 202 169
BciVI GTATCCnnnnn_n'
Cuts at: 0 1602 1929 3150
Size: 1602 327 1221
Fragments arranged by size:
1602 1221 327
BfrBI ATG'CAT
Cuts at: 0 952 3150
Size: 952 2198
BglI GCCn_nnn'nGGC
Cuts at: 0 595 3150
Size: 595 2555
BglII A'GATC_T
Cuts at: 0 1097 3150
Size: 1097 2053
BlpI GC'TnA_GC
Cuts at: 0 68 2155 3150
Size: 68 2087 995
Fragments arranged by size:
2087 995 68
Bme1580I G_kGCm'C
Cuts at: 0 383 992 1012 3105 3150
Size: 383 609 20 2093 45
Fragments arranged by size:
2093 609 383 45 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 205 995 1041 1470 2832 3150
Size: 205 790 46 429 1362 318
Fragments arranged by size:
1362 790 429 318 205 46
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 543 3150
Size: 543 2607
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 1127 1531 2095 3150
Size: 580 547 404 564 1055
Fragments arranged by size:
1055 580 564 547 404
Bpu10I CC'TnA_GC
Cuts at: 0 510 578 3150
Size: 510 68 2572
Fragments arranged by size:
2572 510 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2785 3150
Size: 2785 365
BsaI GGTCTCn'nnnn_
Cuts at: 0 26 1106 1649 3150
Size: 26 1080 543 1501
Fragments arranged by size:
1501 1080 543 26
BsaAI yAC'GTr
Cuts at: 0 2339 3150
Size: 2339 811
BsaBI GATnn'nnATC
Cuts at: 0 2315 2971 3001 3150
Size: 2315 656 30 149
Fragments arranged by size:
2315 656 149 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 324 354 2618 2648 3150
Size: 324 30 2264 30 502
Fragments arranged by size:
2264 502 324 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1124 2563 3040 3150
Size: 1124 1439 477 110
Fragments arranged by size:
1439 1124 477 110
BseYI C'CCAG_C
Cuts at: 0 638 1618 3150
Size: 638 980 1532
Fragments arranged by size:
1532 980 638
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 1715 3150
Size: 347 1368 1435
Fragments arranged by size:
1435 1368 347
BsiEI CG_ry'CG
Cuts at: 0 2964 3150
Size: 2964 186
BsiHKAI G_wGCw'C
Cuts at: 0 240 2354 2771 3150
Size: 240 2114 417 379
Fragments arranged by size:
2114 417 379 240
BsmI GAATG_Cn'
Cuts at: 0 2159 3150
Size: 2159 991
Bsp1286I G_dGCh'C
Cuts at: 0 74 240 383 600 671 992 1012 2354
Size: 74 166 143 217 71 321 20 1342
Cuts at: 2354 2771 3105 3150
Size: 417 334 45
Fragments arranged by size:
1342 417 334 321 217 166 143 74
71 45 20
BspHI T'CATG_A
Cuts at: 0 353 1667 2706 2907 3150
Size: 353 1314 1039 201 243
Fragments arranged by size:
1314 1039 353 243 201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 59 2013 2054 3150
Size: 59 1954 41 1096
Fragments arranged by size:
1954 1096 59 41
BsrDI GCAATG_nn'
Cuts at: 0 441 3150
Size: 441 2709
BsrFI r'CCGG_y
Cuts at: 0 2227 3036 3150
Size: 2227 809 114
Fragments arranged by size:
2227 809 114
BssSI C'ACGA_G
Cuts at: 0 1858 3150
Size: 1858 1292
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2224 3150
Size: 2224 926
BstXI CCAn_nnnn'nTGG
Cuts at: 0 82 1860 3150
Size: 82 1778 1290
Fragments arranged by size:
1778 1290 82
BstYI r'GATC_y
Cuts at: 0 566 1097 3150
Size: 566 531 2053
Fragments arranged by size:
2053 566 531
BtgI C'CryG_G
Cuts at: 0 495 3150
Size: 495 2655
BtsI GCAGTG_nn'
Cuts at: 0 383 2225 3150
Size: 383 1842 925
Fragments arranged by size:
1842 925 383
DraI TTT'AAA
Cuts at: 0 1077 1347 3150
Size: 1077 270 1803
Fragments arranged by size:
1803 1077 270
DraIII CAC_nnn'GTG
Cuts at: 0 138 3150
Size: 138 3012
DrdI GACnn_nn'nnGTC
Cuts at: 0 103 3150
Size: 103 3047
EaeI y'GGCC_r
Cuts at: 0 465 1047 1592 2087 2867 2984 3150
Size: 465 582 545 495 780 117 166
Fragments arranged by size:
780 582 545 495 465 166 117
EarI CTCTTCn'nnn_
Cuts at: 0 852 2776 2831 3150
Size: 852 1924 55 319
Fragments arranged by size:
1924 852 319 55
EcoICRI GAG'CTC
Cuts at: 0 238 2769 3150
Size: 238 2531 381
Fragments arranged by size:
2531 381 238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 1127 1152 1531 2095 2519 2765 3150
Size: 580 547 25 379 564 424 246 385
Fragments arranged by size:
580 564 547 424 385 379 246 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1546 3150
Size: 1546 1604
EcoO109I rG'GnC_Cy
Cuts at: 0 554 3117 3150
Size: 554 2563 33
Fragments arranged by size:
2563 554 33
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1116 1746 2484 2958 3150
Size: 1116 630 738 474 192
Fragments arranged by size:
1116 738 630 474 192
HaeII r_GCGC'y
Cuts at: 0 803 3150
Size: 803 2347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 324 356 676 708 1223 1255 1366 1398
Size: 324 32 320 32 515 32 111 32
Cuts at: 1398 1839 1871 2378 2410 3150
Size: 441 32 507 32 740
Fragments arranged by size:
740 515 507 441 324 320 111 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 849 1164 3150
Size: 849 315 1986
Fragments arranged by size:
1986 849 315
HpaI GTT'AAC
Cuts at: 0 849 3150
Size: 849 2301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1285 2115 3150
Size: 1285 830 1035
Fragments arranged by size:
1285 1035 830
MscI TGG'CCA
Cuts at: 0 467 1049 1594 2089 2986 3150
Size: 467 582 545 495 897 164
Fragments arranged by size:
897 582 545 495 467 164
MslI CAynn'nnrTG
Cuts at: 0 262 1253 3150
Size: 262 991 1897
Fragments arranged by size:
1897 991 262
MspA1I CmG'CkG
Cuts at: 0 312 3020 3150
Size: 312 2708 130
Fragments arranged by size:
2708 312 130
NcoI C'CATG_G
Cuts at: 0 495 3150
Size: 495 2655
NsiI A_TGCA'T
Cuts at: 0 954 3150
Size: 954 2196
NspI r_CATG'y
Cuts at: 0 173 272 1819 3150
Size: 173 99 1547 1331
Fragments arranged by size:
1547 1331 173 99
PasI CC'CwG_GG
Cuts at: 0 1366 3150
Size: 1366 1784
PciI A'CATG_T
Cuts at: 0 169 3150
Size: 169 2981
PflMI CCAn_nnn'nTGG
Cuts at: 0 288 3150
Size: 288 2862
PfoI T'CCnGG_A
Cuts at: 0 1547 3150
Size: 1547 1603
PmlI CAC'GTG
Cuts at: 0 2339 3150
Size: 2339 811
PpuMI rG'GwC_Cy
Cuts at: 0 554 3150
Size: 554 2596
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2800 2832 3150
Size: 2800 32 318
Fragments arranged by size:
2800 318 32
PstI C_TGCA'G
Cuts at: 0 56 1299 2051 2996 3150
Size: 56 1243 752 945 154
Fragments arranged by size:
1243 945 752 154 56
PvuI CG_AT'CG
Cuts at: 0 2964 3150
Size: 2964 186
PvuII CAG'CTG
Cuts at: 0 312 3150
Size: 312 2838
SacI G_AGCT'C
Cuts at: 0 240 2771 3150
Size: 240 2531 379
Fragments arranged by size:
2531 379 240
SapI GCTCTTCn'nnn_
Cuts at: 0 2776 3150
Size: 2776 374
SbfI CC_TGCA'GG
Cuts at: 0 56 3150
Size: 56 3094
ScaI AGT'ACT
Cuts at: 0 1628 2762 3031 3150
Size: 1628 1134 269 119
Fragments arranged by size:
1628 1134 269 119
SexAI A'CCwGG_T
Cuts at: 0 33 3150
Size: 33 3117
SfcI C'TryA_G
Cuts at: 0 52 1295 2014 2047 2992 3150
Size: 52 1243 719 33 945 158
Fragments arranged by size:
1243 945 719 158 52 33
SmaI CCC'GGG
Cuts at: 0 2535 3150
Size: 2535 615
SmlI C'TyrA_G
Cuts at: 0 2764 3150
Size: 2764 386
SspI AAT'ATT
Cuts at: 0 416 1663 3150
Size: 416 1247 1487
Fragments arranged by size:
1487 1247 416
StyI C'CwwG_G
Cuts at: 0 185 495 1583 3150
Size: 185 310 1088 1567
Fragments arranged by size:
1567 1088 310 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 317 2508 3150
Size: 317 2191 642
Fragments arranged by size:
2191 642 317
TatI w'GTAC_w
Cuts at: 0 1125 1626 2760 3029 3077 3150
Size: 1125 501 1134 269 48 73
Fragments arranged by size:
1134 1125 501 269 73 48
XbaI T'CTAG_A
Cuts at: 0 864 1434 3150
Size: 864 570 1716
Fragments arranged by size:
1716 864 570
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 192 502 1602 2979 3150
Size: 192 310 1100 1377 171
Fragments arranged by size:
1377 1100 310 192 171
XmaI C'CCGG_G
Cuts at: 0 2533 3150
Size: 2533 617
XmnI GAAnn'nnTTC
Cuts at: 0 520 1663 2056 2249 2903 3150
Size: 520 1143 393 193 654 247
Fragments arranged by size:
1143 654 520 393 247 193
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApoI AvaI BamHI
BanII BbsI BciVI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BsrFI BssSI BstAPI BstXI BstYI
BtgI BtsI DraI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HpaI
MmeI MscI MslI MspA1I NcoI NsiI NspI PasI
PciI PflMI PfoI PmlI PpuMI PsrI PstI PvuI
PvuII SacI SapI SbfI ScaI SexAI SfcI SmaI
SmlI SspI StyI TaqII TatI XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbvCI BcgI BclI BmgBI BmtI
BsaHI BsaWI BsiWI BsmBI BspEI BsrBI BsrGI BssHII
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI EagI
EciI EcoRI EcoRV FseI FspI FspAI HindIII KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PmeI PpiI PshAI PsiI PspOMI
PspXI RsrII SacII SalI SanDI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI StuI SwaI TstI
Tth111I XhoI ZraI