Restriction map for
pF1KA0029
(Linear) (Six-base) MAPSORT of: KIAA0029.seq Check: 3687 from: 1 to: 2913
KIAA0029.orf
With 182 enzymes: SgfI *
October 24, 2006 15:05 ..
AccI GT'mk_AC
Cuts at: 0 2231 2913
Size: 2231 682
AclI AA'CG_TT
Cuts at: 0 624 2913
Size: 624 2289
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 860 996 1324 1784 1958 2842 2913
Size: 860 136 328 460 174 884 71
Fragments arranged by size:
884 860 460 328 174 136 71
AhdI GACnn_n'nnGTC
Cuts at: 0 2377 2679 2913
Size: 2377 302 234
Fragments arranged by size:
2377 302 234
AlwNI CAG_nnn'CTG
Cuts at: 0 2424 2913
Size: 2424 489
ApoI r'AATT_y
Cuts at: 0 176 331 398 439 577 1844 2218 2913
Size: 176 155 67 41 138 1267 374 695
Fragments arranged by size:
1267 695 374 176 155 138 67 41
AvaI C'yCGr_G
Cuts at: 0 2383 2913
Size: 2383 530
AvrII C'CTAG_G
Cuts at: 0 1245 2913
Size: 1245 1668
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1573 1606 2913
Size: 1573 33 1307
Fragments arranged by size:
1573 1307 33
BanI G'GyrC_C
Cuts at: 0 845 2650 2913
Size: 845 1805 263
Fragments arranged by size:
1805 845 263
BbeI G_GCGC'C
Cuts at: 0 849 2654 2913
Size: 849 1805 259
Fragments arranged by size:
1805 849 259
BbsI GAAGACnn'nnnn_
Cuts at: 0 617 2913
Size: 617 2296
BciVI GTATCCnnnnn_n'
Cuts at: 0 2414 2913
Size: 2414 499
BclI T'GATC_A
Cuts at: 0 516 570 2190 2913
Size: 516 54 1620 723
Fragments arranged by size:
1620 723 516 54
BglII A'GATC_T
Cuts at: 0 321 883 2913
Size: 321 562 2030
Fragments arranged by size:
2030 562 321
BlpI GC'TnA_GC
Cuts at: 0 2303 2913
Size: 2303 610
Bme1580I G_kGCm'C
Cuts at: 0 2151 2913
Size: 2151 762
BmgBI CAC'GTC
Cuts at: 0 2692 2913
Size: 2692 221
BmrI ACTGGGnnnn_n'
Cuts at: 0 1591 2673 2747 2913
Size: 1591 1082 74 166
Fragments arranged by size:
1591 1082 166 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 237 1292 2913
Size: 237 1055 1621
Fragments arranged by size:
1621 1055 237
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 966 1486 1544 1560 2913
Size: 966 520 58 16 1353
Fragments arranged by size:
1353 966 520 58 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 1814 1823 2913
Size: 619 1195 9 1090
Fragments arranged by size:
1195 1090 619 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 743 1256 2913
Size: 743 513 1657
Fragments arranged by size:
1657 743 513
BsaAI yAC'GTr
Cuts at: 0 433 2913
Size: 433 2480
BsaHI Gr'CG_yC
Cuts at: 0 846 2651 2913
Size: 846 1805 262
Fragments arranged by size:
1805 846 262
BsaWI w'CCGG_w
Cuts at: 0 2660 2913
Size: 2660 253
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1558 1588 2543 2573 2749 2779 2913
Size: 1558 30 955 30 176 30 134
Fragments arranged by size:
1558 955 176 134 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1385 1409 1412 1415 2913
Size: 1385 24 3 3 1498
Fragments arranged by size:
1498 1385 24 3 3
BseYI C'CCAG_C
Cuts at: 0 836 2045 2913
Size: 836 1209 868
Fragments arranged by size:
1209 868 836
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2434 2792 2913
Size: 2434 358 121
Fragments arranged by size:
2434 358 121
BsiHKAI G_wGCw'C
Cuts at: 0 1502 2913
Size: 1502 1411
BsmI GAATG_Cn'
Cuts at: 0 1925 2820 2913
Size: 1925 895 93
Fragments arranged by size:
1925 895 93
Bsp1286I G_dGCh'C
Cuts at: 0 1502 2151 2913
Size: 1502 649 762
Fragments arranged by size:
1502 762 649
BspHI T'CATG_A
Cuts at: 0 1367 2913
Size: 1367 1546
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 996 1656 1994 2913
Size: 996 660 338 919
Fragments arranged by size:
996 919 660 338
BsrGI T'GTAC_A
Cuts at: 0 2068 2913
Size: 2068 845
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2815 2913
Size: 2815 98
BstBI TT'CG_AA
Cuts at: 0 975 2913
Size: 975 1938
BstEII G'GTnAC_C
Cuts at: 0 1981 2913
Size: 1981 932
BstXI CCAn_nnnn'nTGG
Cuts at: 0 944 1168 2760 2913
Size: 944 224 1592 153
Fragments arranged by size:
1592 944 224 153
BstYI r'GATC_y
Cuts at: 0 182 321 883 1156 1900 2657 2913
Size: 182 139 562 273 744 757 256
Fragments arranged by size:
757 744 562 273 256 182 139
BtsI GCAGTG_nn'
Cuts at: 0 1636 1822 2170 2232 2422 2721 2726 2913
Size: 1636 186 348 62 190 299 5 187
Fragments arranged by size:
1636 348 299 190 187 186 62 5
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2523 2558 2913
Size: 2523 35 355
Fragments arranged by size:
2523 355 35
DraI TTT'AAA
Cuts at: 0 2643 2913
Size: 2643 270
DraIII CAC_nnn'GTG
Cuts at: 0 2095 2913
Size: 2095 818
DrdI GACnn_nn'nnGTC
Cuts at: 0 2239 2913
Size: 2239 674
EaeI y'GGCC_r
Cuts at: 0 1746 2415 2913
Size: 1746 669 498
Fragments arranged by size:
1746 669 498
EarI CTCTTCn'nnn_
Cuts at: 0 206 725 977 1977 2913
Size: 206 519 252 1000 936
Fragments arranged by size:
1000 936 519 252 206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 860 966 996 1324 1486 1544 1560 1784
Size: 860 106 30 328 162 58 16 224
Cuts at: 1784 1958 2842 2913
Size: 174 884 71
Fragments arranged by size:
884 860 328 224 174 162 106 71
58 30 16
EcoO109I rG'GnC_Cy
Cuts at: 0 2673 2913
Size: 2673 240
EcoRI G'AATT_C
Cuts at: 0 1844 2913
Size: 1844 1069
EcoRV GAT'ATC
Cuts at: 0 1465 2913
Size: 1465 1448
FspI TGC'GCA
Cuts at: 0 1652 2913
Size: 1652 1261
HaeII r_GCGC'y
Cuts at: 0 849 2654 2913
Size: 849 1805 259
Fragments arranged by size:
1805 849 259
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1650 1682 1772 1804 2363 2395 2665 2697
Size: 1650 32 90 32 559 32 270 32
Cuts at: 2697 2749 2781 2913
Size: 52 32 132
Fragments arranged by size:
1650 559 270 132 90 52 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 191 1954 2844 2913
Size: 191 1763 890 69
Fragments arranged by size:
1763 890 191 69
HindIII A'AGCT_T
Cuts at: 0 2623 2913
Size: 2623 290
HpaI GTT'AAC
Cuts at: 0 191 2844 2913
Size: 191 2653 69
Fragments arranged by size:
2653 191 69
KasI G'GCGC_C
Cuts at: 0 845 2650 2913
Size: 845 1805 263
Fragments arranged by size:
1805 845 263
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1689 2913
Size: 1689 1224
MscI TGG'CCA
Cuts at: 0 1748 2417 2913
Size: 1748 669 496
Fragments arranged by size:
1748 669 496
MspA1I CmG'CkG
Cuts at: 0 2074 2307 2806 2913
Size: 2074 233 499 107
Fragments arranged by size:
2074 499 233 107
NarI GG'CG_CC
Cuts at: 0 846 2651 2913
Size: 846 1805 262
Fragments arranged by size:
1805 846 262
PasI CC'CwG_GG
Cuts at: 0 362 2913
Size: 362 2551
PflMI CCAn_nnn'nTGG
Cuts at: 0 2095 2413 2913
Size: 2095 318 500
Fragments arranged by size:
2095 500 318
PfoI T'CCnGG_A
Cuts at: 0 1995 2669 2913
Size: 1995 674 244
Fragments arranged by size:
1995 674 244
PpuMI rG'GwC_Cy
Cuts at: 0 2673 2913
Size: 2673 240
PstI C_TGCA'G
Cuts at: 0 2429 2913
Size: 2429 484
PvuII CAG'CTG
Cuts at: 0 2074 2307 2806 2913
Size: 2074 233 499 107
Fragments arranged by size:
2074 499 233 107
SanDI GG'GwC_CC
Cuts at: 0 2673 2913
Size: 2673 240
SapI GCTCTTCn'nnn_
Cuts at: 0 1977 2913
Size: 1977 936
ScaI AGT'ACT
Cuts at: 0 928 2913
Size: 928 1985
SexAI A'CCwGG_T
Cuts at: 0 671 1947 2091 2913
Size: 671 1276 144 822
Fragments arranged by size:
1276 822 671 144
SfcI C'TryA_G
Cuts at: 0 713 1932 2425 2913
Size: 713 1219 493 488
Fragments arranged by size:
1219 713 493 488
SfoI GGC'GCC
Cuts at: 0 847 2652 2913
Size: 847 1805 261
Fragments arranged by size:
1805 847 261
SmaI CCC'GGG
Cuts at: 0 2385 2913
Size: 2385 528
SmlI C'TyrA_G
Cuts at: 0 634 1829 1838 2913
Size: 634 1195 9 1075
Fragments arranged by size:
1195 1075 634 9
SspI AAT'ATT
Cuts at: 0 758 2182 2238 2913
Size: 758 1424 56 675
Fragments arranged by size:
1424 758 675 56
StyI C'CwwG_G
Cuts at: 0 1245 1698 2541 2913
Size: 1245 453 843 372
Fragments arranged by size:
1245 843 453 372
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 767 2913
Size: 767 2146
TatI w'GTAC_w
Cuts at: 0 926 2010 2068 2429 2913
Size: 926 1084 58 361 484
Fragments arranged by size:
1084 926 484 361 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1558 1590 2913
Size: 1558 32 1323
Fragments arranged by size:
1558 1323 32
XbaI T'CTAG_A
Cuts at: 0 2368 2913
Size: 2368 545
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1705 2071 2913
Size: 1705 366 842
Fragments arranged by size:
1705 842 366
XmaI C'CCGG_G
Cuts at: 0 2383 2913
Size: 2383 530
XmnI GAAnn'nnTTC
Cuts at: 0 614 759 2028 2913
Size: 614 145 1269 885
Fragments arranged by size:
1269 885 614 145
Enzymes that do cut:
AccI AclI AcuI AhdI AlwNI ApoI AvaI AvrII
BaeI BanI BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrGI BstAPI BstBI BstEII BstXI
BstYI BtsI CspCI DraI DraIII DrdI EaeI EarI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI MmeI MscI MspA1I NarI PasI
PflMI PfoI PpuMI PstI PvuII SanDI SapI ScaI
SexAI SfcI SfoI SmaI SmlI SspI StyI TaqII
TatI TstI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI BamHI
BanII BbvCI BcgI BfrBI BglI BmtI Bpu10I BsaBI
BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BssHII
BssSI BstZ17I Bsu36I BtgI BtgZI ClaI EagI EciI
EcoICRI EcoNI FalI FseI FspAI KpnI MfeI MluI
MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI StuI SwaI Tth111I XhoI ZraI