Restriction map for
pF1KSDA0028
(Linear) (Six-base) MAPSORT of: KIAA0028.seq Check: 995 from: 1 to: 2709
KIAA0028.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 645 2709
Size: 645 2064
AccI GT'mk_AC
Cuts at: 0 151 311 2709
Size: 151 160 2398
Fragments arranged by size:
2398 160 151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 1000 1205 1478 1575 2709
Size: 929 71 205 273 97 1134
Fragments arranged by size:
1134 929 273 205 97 71
AflIII A'CryG_T
Cuts at: 0 526 761 2709
Size: 526 235 1948
Fragments arranged by size:
1948 526 235
AleI CACnn'nnGTG
Cuts at: 0 1411 1636 2709
Size: 1411 225 1073
Fragments arranged by size:
1411 1073 225
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2476 2508 2709
Size: 2476 32 201
Fragments arranged by size:
2476 201 32
AlwNI CAG_nnn'CTG
Cuts at: 0 229 2148 2709
Size: 229 1919 561
Fragments arranged by size:
1919 561 229
ApoI r'AATT_y
Cuts at: 0 218 247 1124 2247 2555 2602 2709
Size: 218 29 877 1123 308 47 107
Fragments arranged by size:
1123 877 308 218 107 47 29
AvaI C'yCGr_G
Cuts at: 0 1289 2323 2579 2673 2709
Size: 1289 1034 256 94 36
Fragments arranged by size:
1289 1034 256 94 36
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 691 724 1553 1586 2709
Size: 691 33 829 33 1123
Fragments arranged by size:
1123 829 691 33 33
BanI G'GyrC_C
Cuts at: 0 922 2415 2709
Size: 922 1493 294
Fragments arranged by size:
1493 922 294
BbvCI CC'TCA_GC
Cuts at: 0 2141 2168 2709
Size: 2141 27 541
Fragments arranged by size:
2141 541 27
BciVI GTATCCnnnnn_n'
Cuts at: 0 1554 2709
Size: 1554 1155
BclI T'GATC_A
Cuts at: 0 1737 2531 2709
Size: 1737 794 178
Fragments arranged by size:
1737 794 178
BglI GCCn_nnn'nGGC
Cuts at: 0 898 2709
Size: 898 1811
BglII A'GATC_T
Cuts at: 0 2393 2709
Size: 2393 316
Bme1580I G_kGCm'C
Cuts at: 0 971 1369 1418 2349 2436 2567 2709
Size: 971 398 49 931 87 131 142
Fragments arranged by size:
971 931 398 142 131 87 49
BmgBI CAC'GTC
Cuts at: 0 1314 1907 2709
Size: 1314 593 802
Fragments arranged by size:
1314 802 593
BmrI ACTGGGnnnn_n'
Cuts at: 0 619 627 1124 1371 1387 2709
Size: 619 8 497 247 16 1322
Fragments arranged by size:
1322 619 497 247 16 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2256 2709
Size: 2256 453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 1441 2449 2709
Size: 707 734 1008 260
Fragments arranged by size:
1008 734 707 260
Bpu10I CC'TnA_GC
Cuts at: 0 2141 2168 2709
Size: 2141 27 541
Fragments arranged by size:
2141 541 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1011 2709
Size: 1011 1698
BsaAI yAC'GTr
Cuts at: 0 255 527 933 2709
Size: 255 272 406 1776
Fragments arranged by size:
1776 406 272 255
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 680 710 2182 2212 2709
Size: 680 30 1472 30 497
Fragments arranged by size:
1472 680 497 30 30
BseYI C'CCAG_C
Cuts at: 0 506 583 946 1055 2135 2622 2709
Size: 506 77 363 109 1080 487 87
Fragments arranged by size:
1080 506 487 363 109 87 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 2709
Size: 274 2435
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1304 2709
Size: 1304 1405
Bsp1286I G_dGCh'C
Cuts at: 0 971 1369 1418 2349 2436 2567 2709
Size: 971 398 49 931 87 131 142
Fragments arranged by size:
971 931 398 142 131 87 49
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 351 645 2709
Size: 351 294 2064
Fragments arranged by size:
2064 351 294
BsrDI GCAATG_nn'
Cuts at: 0 154 2286 2709
Size: 154 2132 423
Fragments arranged by size:
2132 423 154
BsrGI T'GTAC_A
Cuts at: 0 1667 2709
Size: 1667 1042
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1019 2476 2709
Size: 1019 1457 233
Fragments arranged by size:
1457 1019 233
BstEII G'GTnAC_C
Cuts at: 0 2214 2709
Size: 2214 495
BstXI CCAn_nnnn'nTGG
Cuts at: 0 443 817 2709
Size: 443 374 1892
Fragments arranged by size:
1892 443 374
BstYI r'GATC_y
Cuts at: 0 1851 2393 2709
Size: 1851 542 316
Fragments arranged by size:
1851 542 316
BstZ17I GTA'TAC
Cuts at: 0 152 2709
Size: 152 2557
Bsu36I CC'TnA_GG
Cuts at: 0 2507 2709
Size: 2507 202
BtgI C'CryG_G
Cuts at: 0 128 374 872 1487 2709
Size: 128 246 498 615 1222
Fragments arranged by size:
1222 615 498 246 128
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1434 2709
Size: 1434 1275
BtsI GCAGTG_nn'
Cuts at: 0 1386 1643 2709
Size: 1386 257 1066
Fragments arranged by size:
1386 1066 257
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2065 2100 2425 2460 2709
Size: 2065 35 325 35 249
Fragments arranged by size:
2065 325 249 35 35
EaeI y'GGCC_r
Cuts at: 0 899 935 1082 1484 1640 2709
Size: 899 36 147 402 156 1069
Fragments arranged by size:
1069 899 402 156 147 36
EarI CTCTTCn'nnn_
Cuts at: 0 44 1291 2709
Size: 44 1247 1418
Fragments arranged by size:
1418 1247 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1801 2709
Size: 1801 908
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 929 1000 1205 1441 1478 1575 2449
Size: 707 222 71 205 236 37 97 874
Cuts at: 2449 2709
Size: 260
Fragments arranged by size:
874 707 260 236 222 205 97 71
37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1518 1795 2709
Size: 1518 277 914
Fragments arranged by size:
1518 914 277
EcoO109I rG'GnC_Cy
Cuts at: 0 606 621 1065 1474 2709
Size: 606 15 444 409 1235
Fragments arranged by size:
1235 606 444 409 15
EcoRI G'AATT_C
Cuts at: 0 1124 2247 2709
Size: 1124 1123 462
Fragments arranged by size:
1124 1123 462
EcoRV GAT'ATC
Cuts at: 0 2025 2709
Size: 2025 684
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1005 2043 2679 2709
Size: 1005 1038 636 30
Fragments arranged by size:
1038 1005 636 30
HaeII r_GCGC'y
Cuts at: 0 2413 2709
Size: 2413 296
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 543 575 2636 2668 2709
Size: 543 32 2061 32 41
Fragments arranged by size:
2061 543 41 32 32
HincII GTy'rAC
Cuts at: 0 615 2709
Size: 615 2094
HindIII A'AGCT_T
Cuts at: 0 2543 2709
Size: 2543 166
HpaI GTT'AAC
Cuts at: 0 615 2709
Size: 615 2094
MluI A'CGCG_T
Cuts at: 0 761 2709
Size: 761 1948
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 2709
Size: 424 2285
MscI TGG'CCA
Cuts at: 0 937 1084 1486 2709
Size: 937 147 402 1223
Fragments arranged by size:
1223 937 402 147
MslI CAynn'nnrTG
Cuts at: 0 305 1246 1411 1636 2709
Size: 305 941 165 225 1073
Fragments arranged by size:
1073 941 305 225 165
MspA1I CmG'CkG
Cuts at: 0 506 1356 2145 2265 2709
Size: 506 850 789 120 444
Fragments arranged by size:
850 789 506 444 120
NcoI C'CATG_G
Cuts at: 0 374 1487 2709
Size: 374 1113 1222
Fragments arranged by size:
1222 1113 374
NspI r_CATG'y
Cuts at: 0 804 1050 1690 2709
Size: 804 246 640 1019
Fragments arranged by size:
1019 804 640 246
PasI CC'CwG_GG
Cuts at: 0 1167 2056 2709
Size: 1167 889 653
Fragments arranged by size:
1167 889 653
PflMI CCAn_nnn'nTGG
Cuts at: 0 380 1167 1399 2709
Size: 380 787 232 1310
Fragments arranged by size:
1310 787 380 232
PfoI T'CCnGG_A
Cuts at: 0 99 2709
Size: 99 2610
PmlI CAC'GTG
Cuts at: 0 933 2709
Size: 933 1776
PpuMI rG'GwC_Cy
Cuts at: 0 621 1065 2709
Size: 621 444 1644
Fragments arranged by size:
1644 621 444
PstI C_TGCA'G
Cuts at: 0 2501 2709
Size: 2501 208
PvuII CAG'CTG
Cuts at: 0 506 2145 2265 2709
Size: 506 1639 120 444
Fragments arranged by size:
1639 506 444 120
SanDI GG'GwC_CC
Cuts at: 0 621 2709
Size: 621 2088
ScaI AGT'ACT
Cuts at: 0 1134 2709
Size: 1134 1575
SfcI C'TryA_G
Cuts at: 0 120 1834 2497 2709
Size: 120 1714 663 212
Fragments arranged by size:
1714 663 212 120
SmaI CCC'GGG
Cuts at: 0 1291 2581 2709
Size: 1291 1290 128
Fragments arranged by size:
1291 1290 128
SmlI C'TyrA_G
Cuts at: 0 990 2709
Size: 990 1719
SphI G_CATG'C
Cuts at: 0 804 2709
Size: 804 1905
SspI AAT'ATT
Cuts at: 0 459 2709
Size: 459 2250
StuI AGG'CCT
Cuts at: 0 211 1790 2405 2709
Size: 211 1579 615 304
Fragments arranged by size:
1579 615 304 211
StyI C'CwwG_G
Cuts at: 0 374 1487 1519 1785 2709
Size: 374 1113 32 266 924
Fragments arranged by size:
1113 924 374 266 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1294 1491 2236 2614 2709
Size: 1294 197 745 378 95
Fragments arranged by size:
1294 745 378 197 95
TatI w'GTAC_w
Cuts at: 0 1132 1667 2189 2709
Size: 1132 535 522 520
Fragments arranged by size:
1132 535 522 520
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 680 712 2014 2046 2326 2358 2415 2447
Size: 680 32 1302 32 280 32 57 32
Cuts at: 2447 2709
Size: 262
Fragments arranged by size:
1302 680 280 262 57 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 2593 2709
Size: 2593 116
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 654 1092 2709
Size: 654 438 1617
Fragments arranged by size:
1617 654 438
XmaI C'CCGG_G
Cuts at: 0 1289 2579 2709
Size: 1289 1290 130
Fragments arranged by size:
1290 1289 130
XmnI GAAnn'nnTTC
Cuts at: 0 2606 2662 2709
Size: 2606 56 47
Fragments arranged by size:
2606 56 47
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AloI AlwNI ApoI
AvaI BaeI BanI BbvCI BciVI BclI BglI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI
BsaXI BseYI BsgI BsmBI Bsp1286I BspMI BsrDI BsrGI
BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI EaeI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HincII HindIII HpaI
MluI MmeI MscI MslI MspA1I NcoI NspI PasI
PflMI PfoI PmlI PpuMI PstI PvuII SanDI ScaI
SfcI SmaI SmlI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
ApaI ApaLI AscI AseI AsiSI AvrII BamHI BanII
BbeI BbsI BcgI BfrBI BlpI BmtI BsaI BsaBI
BsaHI BsaWI BseRI BsiEI BsiHKAI BsiWI BsmI BspEI
BspHI BsrBI BsrFI BssHII BssSI BstBI ClaI DraI
DraIII DrdI EagI EcoICRI FseI FspI FspAI KasI
KpnI MfeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI SwaI XbaI XhoI ZraI