Restriction map for
pF1KA0018
(Linear) (Six-base) MAPSORT of: KIAA0018.seq Check: 7015 from: 1 to: 1548
KIAA0018.orf
With 182 enzymes: SgfI *
October 24, 2006 15:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 219 1548
Size: 219 1329
AccI GT'mk_AC
Cuts at: 0 1220 1548
Size: 1220 328
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 955 1117 1548
Size: 79 876 162 431
Fragments arranged by size:
876 431 162 79
AfeI AGC'GCT
Cuts at: 0 91 186 1548
Size: 91 95 1362
Fragments arranged by size:
1362 95 91
AflIII A'CryG_T
Cuts at: 0 306 1548
Size: 306 1242
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 613 1548
Size: 613 935
AlwNI CAG_nnn'CTG
Cuts at: 0 1191 1355 1407 1548
Size: 1191 164 52 141
Fragments arranged by size:
1191 164 141 52
ApaLI G'TGCA_C
Cuts at: 0 270 1270 1548
Size: 270 1000 278
Fragments arranged by size:
1000 278 270
AvaI C'yCGr_G
Cuts at: 0 1510 1548
Size: 1510 38
BanI G'GyrC_C
Cuts at: 0 278 1074 1164 1548
Size: 278 796 90 384
Fragments arranged by size:
796 384 278 90
BanII G_rGCy'C
Cuts at: 0 8 404 869 1016 1298 1548
Size: 8 396 465 147 282 250
Fragments arranged by size:
465 396 282 250 147 8
BbeI G_GCGC'C
Cuts at: 0 282 1548
Size: 282 1266
BbsI GAAGACnn'nnnn_
Cuts at: 0 331 943 1548
Size: 331 612 605
Fragments arranged by size:
612 605 331
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 498 532 1548
Size: 498 34 1016
Fragments arranged by size:
1016 498 34
BclI T'GATC_A
Cuts at: 0 344 500 1548
Size: 344 156 1048
Fragments arranged by size:
1048 344 156
BglI GCCn_nnn'nGGC
Cuts at: 0 1262 1548
Size: 1262 286
BglII A'GATC_T
Cuts at: 0 1085 1526 1548
Size: 1085 441 22
Fragments arranged by size:
1085 441 22
BlpI GC'TnA_GC
Cuts at: 0 178 1548
Size: 178 1370
Bme1580I G_kGCm'C
Cuts at: 0 274 511 1169 1274 1548
Size: 274 237 658 105 274
Fragments arranged by size:
658 274 274 237 105
BmgBI CAC'GTC
Cuts at: 0 1218 1548
Size: 1218 330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 763 976 1000 1390 1548
Size: 88 675 213 24 390 158
Fragments arranged by size:
675 390 213 158 88 24
Bpu10I CC'TnA_GC
Cuts at: 0 470 1548
Size: 470 1078
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 990 1548
Size: 990 558
BsaI GGTCTCn'nnnn_
Cuts at: 0 1108 1548
Size: 1108 440
BsaAI yAC'GTr
Cuts at: 0 153 307 1146 1548
Size: 153 154 839 402
Fragments arranged by size:
839 402 154 153
BsaBI GATnn'nnATC
Cuts at: 0 688 1548
Size: 688 860
BsaHI Gr'CG_yC
Cuts at: 0 279 1502 1548
Size: 279 1223 46
Fragments arranged by size:
1223 279 46
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1450 1548
Size: 1450 98
BseYI C'CCAG_C
Cuts at: 0 92 660 776 868 1252 1488 1548
Size: 92 568 116 92 384 236 60
Fragments arranged by size:
568 384 236 116 92 92 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 543 1171 1177 1548
Size: 181 362 628 6 371
Fragments arranged by size:
628 371 362 181 6
BsiHKAI G_wGCw'C
Cuts at: 0 80 274 1016 1274 1298 1548
Size: 80 194 742 258 24 250
Fragments arranged by size:
742 258 250 194 80 24
Bsp1286I G_dGCh'C
Cuts at: 0 8 80 274 404 511 869 1016 1169
Size: 8 72 194 130 107 358 147 153
Cuts at: 1169 1274 1298 1548
Size: 105 24 250
Fragments arranged by size:
358 250 194 153 147 130 107 105
72 24 8
BspHI T'CATG_A
Cuts at: 0 341 848 1548
Size: 341 507 700
Fragments arranged by size:
700 507 341
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 219 1548
Size: 219 1329
BsrBI CCG'CTC
Cuts at: 0 123 1548
Size: 123 1425
BssHII G'CGCG_C
Cuts at: 0 29 156 1548
Size: 29 127 1392
Fragments arranged by size:
1392 127 29
BssSI C'ACGA_G
Cuts at: 0 199 769 1548
Size: 199 570 779
Fragments arranged by size:
779 570 199
BstAPI GCAn_nnn'nTGC
Cuts at: 0 210 1158 1191 1548
Size: 210 948 33 357
Fragments arranged by size:
948 357 210 33
BstEII G'GTnAC_C
Cuts at: 0 408 1548
Size: 408 1140
BstYI r'GATC_y
Cuts at: 0 683 1085 1526 1548
Size: 683 402 441 22
Fragments arranged by size:
683 441 402 22
BtgI C'CryG_G
Cuts at: 0 413 904 1548
Size: 413 491 644
Fragments arranged by size:
644 491 413
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 615 1548
Size: 615 933
BtsI GCAGTG_nn'
Cuts at: 0 560 1548
Size: 560 988
DraIII CAC_nnn'GTG
Cuts at: 0 307 798 1146 1149 1548
Size: 307 491 348 3 399
Fragments arranged by size:
491 399 348 307 3
DrdI GACnn_nn'nnGTC
Cuts at: 0 1216 1548
Size: 1216 332
EaeI y'GGCC_r
Cuts at: 0 20 674 1548
Size: 20 654 874
Fragments arranged by size:
874 654 20
EarI CTCTTCn'nnn_
Cuts at: 0 43 115 1548
Size: 43 72 1433
Fragments arranged by size:
1433 72 43
EcoICRI GAG'CTC
Cuts at: 0 1014 1296 1548
Size: 1014 282 252
Fragments arranged by size:
1014 282 252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 88 763 955 976 1000 1117 1390
Size: 79 9 675 192 21 24 117 273
Cuts at: 1390 1548
Size: 158
Fragments arranged by size:
675 273 192 158 117 79 24 21
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 823 1183 1359 1548
Size: 823 360 176 189
Fragments arranged by size:
823 360 189 176
EcoO109I rG'GnC_Cy
Cuts at: 0 738 951 1188 1352 1548
Size: 738 213 237 164 196
Fragments arranged by size:
738 237 213 196 164
EcoRV GAT'ATC
Cuts at: 0 141 1548
Size: 141 1407
FspI TGC'GCA
Cuts at: 0 1123 1548
Size: 1123 425
HaeII r_GCGC'y
Cuts at: 0 93 188 282 1548
Size: 93 95 94 1266
Fragments arranged by size:
1266 95 94 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 511 543 1062 1094 1548
Size: 511 32 519 32 454
Fragments arranged by size:
519 511 454 32 32
KasI G'GCGC_C
Cuts at: 0 278 1548
Size: 278 1270
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 573 1548
Size: 573 975
MslI CAynn'nnrTG
Cuts at: 0 1162 1548
Size: 1162 386
MspA1I CmG'CkG
Cuts at: 0 679 780 1368 1548
Size: 679 101 588 180
Fragments arranged by size:
679 588 180 101
NarI GG'CG_CC
Cuts at: 0 279 1548
Size: 279 1269
NcoI C'CATG_G
Cuts at: 0 413 1548
Size: 413 1135
NdeI CA'TA_TG
Cuts at: 0 1317 1548
Size: 1317 231
NspI r_CATG'y
Cuts at: 0 922 1161 1548
Size: 922 239 387
Fragments arranged by size:
922 387 239
PasI CC'CwG_GG
Cuts at: 0 1107 1548
Size: 1107 441
PflMI CCAn_nnn'nTGG
Cuts at: 0 449 798 1548
Size: 449 349 750
Fragments arranged by size:
750 449 349
PfoI T'CCnGG_A
Cuts at: 0 1016 1548
Size: 1016 532
PmlI CAC'GTG
Cuts at: 0 1146 1548
Size: 1146 402
PpuMI rG'GwC_Cy
Cuts at: 0 1352 1548
Size: 1352 196
PshAI GACnn'nnGTC
Cuts at: 0 844 1548
Size: 844 704
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 529 561 1548
Size: 529 32 987
Fragments arranged by size:
987 529 32
PstI C_TGCA'G
Cuts at: 0 1184 1548
Size: 1184 364
PvuII CAG'CTG
Cuts at: 0 1368 1548
Size: 1368 180
SacI G_AGCT'C
Cuts at: 0 1016 1298 1548
Size: 1016 282 250
Fragments arranged by size:
1016 282 250
SapI GCTCTTCn'nnn_
Cuts at: 0 43 1548
Size: 43 1505
SfcI C'TryA_G
Cuts at: 0 1180 1548
Size: 1180 368
SfoI GGC'GCC
Cuts at: 0 280 1548
Size: 280 1268
SmlI C'TyrA_G
Cuts at: 0 969 1548
Size: 969 579
StuI AGG'CCT
Cuts at: 0 1265 1548
Size: 1265 283
StyI C'CwwG_G
Cuts at: 0 404 413 1548
Size: 404 9 1135
Fragments arranged by size:
1135 404 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 39 84 153 781 1548
Size: 39 45 69 628 767
Fragments arranged by size:
767 628 69 45 39
TatI w'GTAC_w
Cuts at: 0 317 959 1548
Size: 317 642 589
Fragments arranged by size:
642 589 317
XmnI GAAnn'nnTTC
Cuts at: 0 793 1548
Size: 793 755
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlfI AlwNI ApaLI
AvaI BanI BanII BbeI BbsI BcgI BclI BglI
BglII BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BssHII BssSI BstAPI BstEII BstYI
BtgI BtgZI BtsI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I KasI
MmeI MslI MspA1I NarI NcoI NdeI NspI PasI
PflMI PfoI PmlI PpuMI PshAI PsrI PstI PvuII
SacI SapI SfcI SfoI SmlI StuI StyI TaqII
TatI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AloI
ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BciVI BfrBI BmrI BmtI BplI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BsrDI BsrFI BsrGI
BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraI EagI
EciI EcoRI FalI FseI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI NaeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PpiI PsiI PspOMI
PspXI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI