Restriction map for
pF1KSDA0014
(Linear) (Six-base) MAPSORT of: KIAA0014.seq Check: 2839 from: 1 to: 1479
KIAA0014.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 21 1479
Size: 21 1458
Acc65I G'GTAC_C
Cuts at: 0 1187 1479
Size: 1187 292
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1455 1479
Size: 1455 24
AflIII A'CryG_T
Cuts at: 0 155 726 1479
Size: 155 571 753
Fragments arranged by size:
753 571 155
AhdI GACnn_n'nnGTC
Cuts at: 0 1121 1479
Size: 1121 358
AleI CACnn'nnGTG
Cuts at: 0 10 1479
Size: 10 1469
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 592 1479
Size: 592 887
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 511 543 1479
Size: 511 32 936
Fragments arranged by size:
936 511 32
AlwNI CAG_nnn'CTG
Cuts at: 0 36 66 189 301 472 648 747 1074
Size: 36 30 123 112 171 176 99 327
Cuts at: 1074 1479
Size: 405
Fragments arranged by size:
405 327 176 171 123 112 99 36
30
ApaLI G'TGCA_C
Cuts at: 0 1279 1479
Size: 1279 200
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 142 175 1479
Size: 142 33 1304
Fragments arranged by size:
1304 142 33
BamHI G'GATC_C
Cuts at: 0 387 1479
Size: 387 1092
BanI G'GyrC_C
Cuts at: 0 394 1059 1187 1479
Size: 394 665 128 292
Fragments arranged by size:
665 394 292 128
BanII G_rGCy'C
Cuts at: 0 866 877 928 997 1479
Size: 866 11 51 69 482
Fragments arranged by size:
866 482 69 51 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 133 1479
Size: 133 1346
BbvCI CC'TCA_GC
Cuts at: 0 605 1479
Size: 605 874
BclI T'GATC_A
Cuts at: 0 716 1479
Size: 716 763
BfrBI ATG'CAT
Cuts at: 0 440 1479
Size: 440 1039
BglI GCCn_nnn'nGGC
Cuts at: 0 61 704 1202 1479
Size: 61 643 498 277
Fragments arranged by size:
643 498 277 61
BlpI GC'TnA_GC
Cuts at: 0 865 910 1262 1479
Size: 865 45 352 217
Fragments arranged by size:
865 352 217 45
Bme1580I G_kGCm'C
Cuts at: 0 207 222 1283 1422 1479
Size: 207 15 1061 139 57
Fragments arranged by size:
1061 207 139 57 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 952 961 1129 1363 1479
Size: 952 9 168 234 116
Fragments arranged by size:
952 234 168 116 9
Bpu10I CC'TnA_GC
Cuts at: 0 605 1479
Size: 605 874
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 997 1213 1479
Size: 39 958 216 266
Fragments arranged by size:
958 266 216 39
BsaAI yAC'GTr
Cuts at: 0 158 729 1479
Size: 158 571 750
Fragments arranged by size:
750 571 158
BsaWI w'CCGG_w
Cuts at: 0 1184 1479
Size: 1184 295
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 511 541 1479
Size: 511 30 938
Fragments arranged by size:
938 511 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 867 1375 1479
Size: 867 508 104
Fragments arranged by size:
867 508 104
BseYI C'CCAG_C
Cuts at: 0 446 1139 1479
Size: 446 693 340
Fragments arranged by size:
693 446 340
BsiHKAI G_wGCw'C
Cuts at: 0 25 866 960 1283 1430 1479
Size: 25 841 94 323 147 49
Fragments arranged by size:
841 323 147 94 49 25
Bsp1286I G_dGCh'C
Cuts at: 0 25 207 222 866 877 928 960 997
Size: 25 182 15 644 11 51 32 37
Cuts at: 997 1283 1422 1430 1479
Size: 286 139 8 49
Fragments arranged by size:
644 286 182 139 51 49 37 32
25 15 11 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 21 518 605 620 686 1479
Size: 21 497 87 15 66 793
Fragments arranged by size:
793 497 87 66 21 15
BsrBI CCG'CTC
Cuts at: 0 234 1479
Size: 234 1245
BsrDI GCAATG_nn'
Cuts at: 0 440 1479
Size: 440 1039
BstAPI GCAn_nnn'nTGC
Cuts at: 0 36 339 1398 1479
Size: 36 303 1059 81
Fragments arranged by size:
1059 303 81 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 492 1479
Size: 492 987
BstYI r'GATC_y
Cuts at: 0 387 1479
Size: 387 1092
Bsu36I CC'TnA_GG
Cuts at: 0 968 1410 1479
Size: 968 442 69
Fragments arranged by size:
968 442 69
BtgI C'CryG_G
Cuts at: 0 491 797 1217 1436 1479
Size: 491 306 420 219 43
Fragments arranged by size:
491 420 306 219 43
BtsI GCAGTG_nn'
Cuts at: 0 46 207 1479
Size: 46 161 1272
Fragments arranged by size:
1272 161 46
DraIII CAC_nnn'GTG
Cuts at: 0 771 1479
Size: 771 708
EaeI y'GGCC_r
Cuts at: 0 749 1148 1318 1479
Size: 749 399 170 161
Fragments arranged by size:
749 399 170 161
EarI CTCTTCn'nnn_
Cuts at: 0 94 1479
Size: 94 1385
EcoICRI GAG'CTC
Cuts at: 0 864 1479
Size: 864 615
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 952 961 1129 1363 1455 1479
Size: 952 9 168 234 92 24
Fragments arranged by size:
952 234 168 92 24 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 226 544 1342 1479
Size: 226 318 798 137
Fragments arranged by size:
798 318 226 137
EcoO109I rG'GnC_Cy
Cuts at: 0 63 594 609 972 1479
Size: 63 531 15 363 507
Fragments arranged by size:
531 507 363 63 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 129 1479
Size: 129 1350
FspI TGC'GCA
Cuts at: 0 622 1479
Size: 622 857
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1107 1139 1479
Size: 1107 32 340
Fragments arranged by size:
1107 340 32
KpnI G_GTAC'C
Cuts at: 0 1191 1479
Size: 1191 288
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1001 1479
Size: 1001 478
MscI TGG'CCA
Cuts at: 0 751 1150 1479
Size: 751 399 329
Fragments arranged by size:
751 399 329
MslI CAynn'nnrTG
Cuts at: 0 10 403 490 1479
Size: 10 393 87 989
Fragments arranged by size:
989 393 87 10
MspA1I CmG'CkG
Cuts at: 0 52 171 189 576 906 1056 1143 1398
Size: 52 119 18 387 330 150 87 255
Cuts at: 1398 1479
Size: 81
Fragments arranged by size:
387 330 255 150 119 87 81 52
18
NcoI C'CATG_G
Cuts at: 0 1217 1479
Size: 1217 262
NsiI A_TGCA'T
Cuts at: 0 442 1479
Size: 442 1037
NspI r_CATG'y
Cuts at: 0 255 337 408 440 1003 1479
Size: 255 82 71 32 563 476
Fragments arranged by size:
563 476 255 82 71 32
PflMI CCAn_nnn'nTGG
Cuts at: 0 747 1146 1479
Size: 747 399 333
Fragments arranged by size:
747 399 333
PmlI CAC'GTG
Cuts at: 0 158 729 1479
Size: 158 571 750
Fragments arranged by size:
750 571 158
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 511 543 1479
Size: 511 32 936
Fragments arranged by size:
936 511 32
PpuMI rG'GwC_Cy
Cuts at: 0 594 609 972 1479
Size: 594 15 363 507
Fragments arranged by size:
594 507 363 15
PstI C_TGCA'G
Cuts at: 0 41 214 230 328 644 923 1479
Size: 41 173 16 98 316 279 556
Fragments arranged by size:
556 316 279 173 98 41 16
PvuII CAG'CTG
Cuts at: 0 52 189 906 1056 1143 1398 1479
Size: 52 137 717 150 87 255 81
Fragments arranged by size:
717 255 150 137 87 81 52
SacI G_AGCT'C
Cuts at: 0 866 1479
Size: 866 613
SbfI CC_TGCA'GG
Cuts at: 0 230 1479
Size: 230 1249
SfcI C'TryA_G
Cuts at: 0 37 193 210 226 324 640 919 1479
Size: 37 156 17 16 98 316 279 560
Fragments arranged by size:
560 316 279 156 98 37 17 16
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 704 1479
Size: 704 775
SmlI C'TyrA_G
Cuts at: 0 18 1012 1228 1479
Size: 18 994 216 251
Fragments arranged by size:
994 251 216 18
SphI G_CATG'C
Cuts at: 0 255 337 1479
Size: 255 82 1142
Fragments arranged by size:
1142 255 82
StuI AGG'CCT
Cuts at: 0 1348 1479
Size: 1348 131
StyI C'CwwG_G
Cuts at: 0 1191 1217 1479
Size: 1191 26 262
Fragments arranged by size:
1191 262 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1153 1479
Size: 1153 326
TatI w'GTAC_w
Cuts at: 0 415 1479
Size: 415 1064
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1145 1177 1479
Size: 1145 32 302
Fragments arranged by size:
1145 302 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 454 772 1479
Size: 454 318 707
Fragments arranged by size:
707 454 318
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI AleI AlfI AloI
AlwNI ApaLI BaeI BamHI BanI BanII BbsI BbvCI
BclI BfrBI BglI BlpI Bme1580I BpmI Bpu10I BpuEI
BsaAI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BstAPI BstXI BstYI Bsu36I BtgI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI Hin4I KpnI MmeI MscI MslI MspA1I NcoI
NsiI NspI PflMI PmlI PpiI PpuMI PstI PvuII
SacI SbfI SfcI SfiI SmlI SphI StuI StyI
TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI ApaI ApoI
AscI AseI AsiSI AvaI AvrII BbeI BcgI BciVI
BglII BmgBI BmrI BmtI BplI BsaI BsaBI BsaHI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI DrdI EagI EciI EcoRI EcoRV FseI
FspAI HaeII HincII HindIII HpaI KasI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PfoI PmeI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI ScaI
SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI