Restriction map for
pF1KA0010
(Linear) (Six-base) MAPSORT of: KIAA0010.seq Check: 4139 from: 1 to: 3249
KIAA0010.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3157 3249
Size: 3157 92
Acc65I G'GTAC_C
Cuts at: 0 1317 1425 3249
Size: 1317 108 1824
Fragments arranged by size:
1824 1317 108
AclI AA'CG_TT
Cuts at: 0 336 3249
Size: 336 2913
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1207 1420 2362 2638 3055 3184 3249
Size: 1207 213 942 276 417 129 65
Fragments arranged by size:
1207 942 417 276 213 129 65
AflIII A'CryG_T
Cuts at: 0 1944 3249
Size: 1944 1305
AleI CACnn'nnGTG
Cuts at: 0 2665 3249
Size: 2665 584
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 478 3175 3249
Size: 478 2697 74
Fragments arranged by size:
2697 478 74
AlwNI CAG_nnn'CTG
Cuts at: 0 303 3149 3249
Size: 303 2846 100
Fragments arranged by size:
2846 303 100
ApaI G_GGCC'C
Cuts at: 0 996 3249
Size: 996 2253
ApaLI G'TGCA_C
Cuts at: 0 2260 2897 3249
Size: 2260 637 352
Fragments arranged by size:
2260 637 352
ApoI r'AATT_y
Cuts at: 0 590 704 1552 1857 2604 2707 2876 3249
Size: 590 114 848 305 747 103 169 373
Fragments arranged by size:
848 747 590 373 305 169 114 103
AseI AT'TA_AT
Cuts at: 0 2168 2887 3249
Size: 2168 719 362
Fragments arranged by size:
2168 719 362
AvaI C'yCGr_G
Cuts at: 0 686 2845 3172 3249
Size: 686 2159 327 77
Fragments arranged by size:
2159 686 327 77
BamHI G'GATC_C
Cuts at: 0 2252 3249
Size: 2252 997
BanI G'GyrC_C
Cuts at: 0 1317 1425 3249
Size: 1317 108 1824
Fragments arranged by size:
1824 1317 108
BanII G_rGCy'C
Cuts at: 0 887 996 3249
Size: 887 109 2253
Fragments arranged by size:
2253 887 109
BbsI GAAGACnn'nnnn_
Cuts at: 0 332 1254 2636 2976 3249
Size: 332 922 1382 340 273
Fragments arranged by size:
1382 922 340 332 273
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1637 1671 3249
Size: 1637 34 1578
Fragments arranged by size:
1637 1578 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3098 3249
Size: 3098 151
BclI T'GATC_A
Cuts at: 0 1409 2865 3249
Size: 1409 1456 384
Fragments arranged by size:
1456 1409 384
BfrBI ATG'CAT
Cuts at: 0 1654 1769 3249
Size: 1654 115 1480
Fragments arranged by size:
1654 1480 115
BglII A'GATC_T
Cuts at: 0 2099 3249
Size: 2099 1150
BlpI GC'TnA_GC
Cuts at: 0 3244 3249
Size: 3244 5
Bme1580I G_kGCm'C
Cuts at: 0 996 2264 2901 3249
Size: 996 1268 637 348
Fragments arranged by size:
1268 996 637 348
BmgBI CAC'GTC
Cuts at: 0 2001 2559 3249
Size: 2001 558 690
Fragments arranged by size:
2001 690 558
BmtI G_CTAG'C
Cuts at: 0 566 3249
Size: 566 2683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2207 3009 3249
Size: 2207 802 240
Fragments arranged by size:
2207 802 240
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1119 3145 3249
Size: 1119 2026 104
Fragments arranged by size:
2026 1119 104
BsaI GGTCTCn'nnnn_
Cuts at: 0 632 3249
Size: 632 2617
BsaWI w'CCGG_w
Cuts at: 0 756 2744 3249
Size: 756 1988 505
Fragments arranged by size:
1988 756 505
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2563 2593 3100 3130 3249
Size: 2563 30 507 30 119
Fragments arranged by size:
2563 507 119 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 811 1120 1123 1270 1866 2656 3249
Size: 811 309 3 147 596 790 593
Fragments arranged by size:
811 790 596 593 309 147 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1321 3249
Size: 1321 1928
BsiHKAI G_wGCw'C
Cuts at: 0 945 2264 2901 3249
Size: 945 1319 637 348
Fragments arranged by size:
1319 945 637 348
BsmI GAATG_Cn'
Cuts at: 0 515 900 1186 2474 3249
Size: 515 385 286 1288 775
Fragments arranged by size:
1288 775 515 385 286
Bsp1286I G_dGCh'C
Cuts at: 0 887 945 996 2264 2901 3249
Size: 887 58 51 1268 637 348
Fragments arranged by size:
1268 887 637 348 58 51
BspEI T'CCGG_A
Cuts at: 0 756 3249
Size: 756 2493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 446 2682 3157 3167 3249
Size: 446 2236 475 10 82
Fragments arranged by size:
2236 475 446 82 10
BsrBI CCG'CTC
Cuts at: 0 3129 3249
Size: 3129 120
BsrDI GCAATG_nn'
Cuts at: 0 260 730 3249
Size: 260 470 2519
Fragments arranged by size:
2519 470 260
BssSI C'ACGA_G
Cuts at: 0 1848 3249
Size: 1848 1401
BstBI TT'CG_AA
Cuts at: 0 11 3249
Size: 11 3238
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1877 3249
Size: 1877 1372
BstYI r'GATC_y
Cuts at: 0 400 2099 2252 2792 3249
Size: 400 1699 153 540 457
Fragments arranged by size:
1699 540 457 400 153
Bsu36I CC'TnA_GG
Cuts at: 0 2591 3249
Size: 2591 658
BtgI C'CryG_G
Cuts at: 0 1271 2277 3249
Size: 1271 1006 972
Fragments arranged by size:
1271 1006 972
BtsI GCAGTG_nn'
Cuts at: 0 2802 3249
Size: 2802 447
DraI TTT'AAA
Cuts at: 0 2330 3249
Size: 2330 919
DraIII CAC_nnn'GTG
Cuts at: 0 2766 3249
Size: 2766 483
DrdI GACnn_nn'nnGTC
Cuts at: 0 2728 3249
Size: 2728 521
EarI CTCTTCn'nnn_
Cuts at: 0 1613 2614 3249
Size: 1613 1001 635
Fragments arranged by size:
1613 1001 635
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1977 2785 2806 3249
Size: 1977 808 21 443
Fragments arranged by size:
1977 808 443 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1207 1420 2207 2362 2638 3009 3055 3184
Size: 1207 213 787 155 276 371 46 129
Cuts at: 3184 3249
Size: 65
Fragments arranged by size:
1207 787 371 276 213 155 129 65
46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1000 2683 3249
Size: 1000 1683 566
Fragments arranged by size:
1683 1000 566
EcoO109I rG'GnC_Cy
Cuts at: 0 992 993 2823 2922 3249
Size: 992 1 1830 99 327
Fragments arranged by size:
1830 992 327 99 1
EcoRI G'AATT_C
Cuts at: 0 1552 3249
Size: 1552 1697
EcoRV GAT'ATC
Cuts at: 0 1445 3249
Size: 1445 1804
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1348 3249
Size: 1348 1901
FspI TGC'GCA
Cuts at: 0 863 3249
Size: 863 2386
HaeII r_GCGC'y
Cuts at: 0 254 853 2741 3249
Size: 254 599 1888 508
Fragments arranged by size:
1888 599 508 254
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1117 1149 2084 2116 2914 2946 3050 3082
Size: 1117 32 935 32 798 32 104 32
Cuts at: 3082 3249
Size: 167
Fragments arranged by size:
1117 935 798 167 104 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1400 3249
Size: 1400 1849
HindIII A'AGCT_T
Cuts at: 0 652 3249
Size: 652 2597
KpnI G_GTAC'C
Cuts at: 0 1321 1429 3249
Size: 1321 108 1820
Fragments arranged by size:
1820 1321 108
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 3143 3249
Size: 374 2769 106
Fragments arranged by size:
2769 374 106
MslI CAynn'nnrTG
Cuts at: 0 1270 1294 1774 2500 2665 3151 3249
Size: 1270 24 480 726 165 486 98
Fragments arranged by size:
1270 726 486 480 165 98 24
MspA1I CmG'CkG
Cuts at: 0 1983 2022 3232 3249
Size: 1983 39 1210 17
Fragments arranged by size:
1983 1210 39 17
NcoI C'CATG_G
Cuts at: 0 1271 2277 3249
Size: 1271 1006 972
Fragments arranged by size:
1271 1006 972
NheI G'CTAG_C
Cuts at: 0 562 3249
Size: 562 2687
NsiI A_TGCA'T
Cuts at: 0 1656 1771 3249
Size: 1656 115 1478
Fragments arranged by size:
1656 1478 115
NspI r_CATG'y
Cuts at: 0 419 1658 1948 2499 3249
Size: 419 1239 290 551 750
Fragments arranged by size:
1239 750 551 419 290
PacI TTA_AT'TAA
Cuts at: 0 365 3249
Size: 365 2884
PciI A'CATG_T
Cuts at: 0 1944 3249
Size: 1944 1305
PflMI CCAn_nnn'nTGG
Cuts at: 0 2283 2766 3249
Size: 2283 483 483
Fragments arranged by size:
2283 483 483
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 906 938 3249
Size: 906 32 2311
Fragments arranged by size:
2311 906 32
PpuMI rG'GwC_Cy
Cuts at: 0 2922 3249
Size: 2922 327
PspOMI G'GGCC_C
Cuts at: 0 992 3249
Size: 992 2257
PspXI vC'TCGA_Gb
Cuts at: 0 2845 3249
Size: 2845 404
PstI C_TGCA'G
Cuts at: 0 1037 1988 2964 3249
Size: 1037 951 976 285
Fragments arranged by size:
1037 976 951 285
SapI GCTCTTCn'nnn_
Cuts at: 0 1613 2614 3249
Size: 1613 1001 635
Fragments arranged by size:
1613 1001 635
SexAI A'CCwGG_T
Cuts at: 0 1229 3249
Size: 1229 2020
SfcI C'TryA_G
Cuts at: 0 174 695 1033 1338 1984 2775 2960 3249
Size: 174 521 338 305 646 791 185 289
Fragments arranged by size:
791 646 521 338 305 289 185 174
SmlI C'TyrA_G
Cuts at: 0 686 1134 2845 3124 3249
Size: 686 448 1711 279 125
Fragments arranged by size:
1711 686 448 279 125
SphI G_CATG'C
Cuts at: 0 1658 3249
Size: 1658 1591
SspI AAT'ATT
Cuts at: 0 196 673 3249
Size: 196 477 2576
Fragments arranged by size:
2576 477 196
StyI C'CwwG_G
Cuts at: 0 35 45 1271 2277 3249
Size: 35 10 1226 1006 972
Fragments arranged by size:
1226 1006 972 35 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2563 2595 3249
Size: 2563 32 654
Fragments arranged by size:
2563 654 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1998 2556 3249
Size: 1998 558 693
Fragments arranged by size:
1998 693 558
XhoI C'TCGA_G
Cuts at: 0 686 2845 3249
Size: 686 2159 404
Fragments arranged by size:
2159 686 404
XmnI GAAnn'nnTTC
Cuts at: 0 2393 3249
Size: 2393 856
Enzymes that do cut:
AarI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AseI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmtI BpmI BpuEI BsaI BsaWI BsaXI BseRI
BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI
BssSI BstBI BstXI BstYI Bsu36I BtgI BtsI DraI
DraIII DrdI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KpnI
MmeI MslI MspA1I NcoI NheI NsiI NspI PacI
PciI PflMI PpiI PpuMI PspOMI PspXI PstI SapI
SexAI SfcI SmlI SphI SspI StyI TstI XcmI
XhoI XmnI
Enzymes that do not cut:
AatII AccI AfeI AflII AgeI AhdI AloI AscI
AsiSI AvrII BaeI BbeI BbvCI BglI BmrI BplI
Bpu10I BsaAI BsaBI BsaHI BseYI BsiEI BsiWI BsmBI
BspHI BsrFI BsrGI BssHII BstAPI BstEII BstZ17I BtgZI
ClaI CspCI EaeI EagI EcoICRI FseI FspAI HpaI
KasI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NotI NruI PasI PfoI PmeI PmlI PshAI PsiI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI SwaI TaqII TatI Tth111I XbaI
XmaI ZraI