Restriction map for
pF1KSDF0187
(Linear) (Six-base) MAPSORT of: FLJ00187.seq Check: 3708 from: 1 to: 1689
FLJ00187.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 250 1056 1121 1224 1308 1392 1689
Size: 159 91 806 65 103 84 84 297
Fragments arranged by size:
806 297 159 103 91 84 84 65
AflIII A'CryG_T
Cuts at: 0 107 1689
Size: 107 1582
AhdI GACnn_n'nnGTC
Cuts at: 0 424 1689
Size: 424 1265
AleI CACnn'nnGTG
Cuts at: 0 1133 1689
Size: 1133 556
AlwNI CAG_nnn'CTG
Cuts at: 0 252 285 735 1689
Size: 252 33 450 954
Fragments arranged by size:
954 450 252 33
ApaI G_GGCC'C
Cuts at: 0 209 1689
Size: 209 1480
ApaLI G'TGCA_C
Cuts at: 0 1554 1689
Size: 1554 135
ApoI r'AATT_y
Cuts at: 0 1442 1526 1689
Size: 1442 84 163
Fragments arranged by size:
1442 163 84
AvaI C'yCGr_G
Cuts at: 0 209 498 1535 1689
Size: 209 289 1037 154
Fragments arranged by size:
1037 289 209 154
BamHI G'GATC_C
Cuts at: 0 480 1274 1689
Size: 480 794 415
Fragments arranged by size:
794 480 415
BanI G'GyrC_C
Cuts at: 0 471 1689
Size: 471 1218
BanII G_rGCy'C
Cuts at: 0 209 335 582 1084 1420 1689
Size: 209 126 247 502 336 269
Fragments arranged by size:
502 336 269 247 209 126
BbsI GAAGACnn'nnnn_
Cuts at: 0 107 401 1689
Size: 107 294 1288
Fragments arranged by size:
1288 294 107
BfrBI ATG'CAT
Cuts at: 0 1660 1689
Size: 1660 29
Bme1580I G_kGCm'C
Cuts at: 0 209 1558 1689
Size: 209 1349 131
Fragments arranged by size:
1349 209 131
BmrI ACTGGGnnnn_n'
Cuts at: 0 522 1689
Size: 522 1167
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 235 849 1647 1689
Size: 235 614 798 42
Fragments arranged by size:
798 614 235 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 307 319 733 1220 1388 1689
Size: 131 176 12 414 487 168 301
Fragments arranged by size:
487 414 301 176 168 131 12
BsaI GGTCTCn'nnnn_
Cuts at: 0 623 1689
Size: 623 1066
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 129 159 1689
Size: 129 30 1530
Fragments arranged by size:
1530 129 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 134 388 838 973 1689
Size: 134 254 450 135 716
Fragments arranged by size:
716 450 254 135 134
BseYI C'CCAG_C
Cuts at: 0 1644 1689
Size: 1644 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 587 632 1689
Size: 32 555 45 1057
Fragments arranged by size:
1057 555 45 32
BsiHKAI G_wGCw'C
Cuts at: 0 335 582 1084 1420 1558 1689
Size: 335 247 502 336 138 131
Fragments arranged by size:
502 336 335 247 138 131
BsmBI CGTCTCn'nnnn_
Cuts at: 0 554 1689
Size: 554 1135
Bsp1286I G_dGCh'C
Cuts at: 0 209 335 582 1084 1420 1558 1689
Size: 209 126 247 502 336 138 131
Fragments arranged by size:
502 336 247 209 138 131 126
BspHI T'CATG_A
Cuts at: 0 612 1296 1689
Size: 612 684 393
Fragments arranged by size:
684 612 393
BsrDI GCAATG_nn'
Cuts at: 0 680 1689
Size: 680 1009
BssSI C'ACGA_G
Cuts at: 0 517 575 1158 1689
Size: 517 58 583 531
Fragments arranged by size:
583 531 517 58
BstYI r'GATC_y
Cuts at: 0 281 480 886 1274 1689
Size: 281 199 406 388 415
Fragments arranged by size:
415 406 388 281 199
Bsu36I CC'TnA_GG
Cuts at: 0 956 1689
Size: 956 733
BtgI C'CryG_G
Cuts at: 0 26 1112 1689
Size: 26 1086 577
Fragments arranged by size:
1086 577 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 652 1689
Size: 652 1037
DraIII CAC_nnn'GTG
Cuts at: 0 1562 1689
Size: 1562 127
EaeI y'GGCC_r
Cuts at: 0 256 1689
Size: 256 1433
EarI CTCTTCn'nnn_
Cuts at: 0 80 587 1689
Size: 80 507 1102
Fragments arranged by size:
1102 507 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 154 1168 1689
Size: 154 1014 521
Fragments arranged by size:
1014 521 154
EcoICRI GAG'CTC
Cuts at: 0 333 580 1082 1418 1689
Size: 333 247 502 336 271
Fragments arranged by size:
502 336 333 271 247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 159 250 307 319 733 1056 1121
Size: 131 28 91 57 12 414 323 65
Cuts at: 1121 1220 1224 1308 1388 1392 1689
Size: 99 4 84 80 4 297
Fragments arranged by size:
414 323 297 131 99 91 84 80
65 57 28 12 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 325 1689
Size: 325 1364
EcoO109I rG'GnC_Cy
Cuts at: 0 206 426 1689
Size: 206 220 1263
Fragments arranged by size:
1263 220 206
EcoRI G'AATT_C
Cuts at: 0 1442 1526 1689
Size: 1442 84 163
Fragments arranged by size:
1442 163 84
HaeII r_GCGC'y
Cuts at: 0 183 1689
Size: 183 1506
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 410 442 459 491 847 879 1503 1535
Size: 410 32 17 32 356 32 624 32
Cuts at: 1535 1689
Size: 154
Fragments arranged by size:
624 410 356 154 32 32 32 32
17
HincII GTy'rAC
Cuts at: 0 830 1689
Size: 830 859
HindIII A'AGCT_T
Cuts at: 0 1068 1487 1689
Size: 1068 419 202
Fragments arranged by size:
1068 419 202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1109 1277 1689
Size: 1109 168 412
Fragments arranged by size:
1109 412 168
MslI CAynn'nnrTG
Cuts at: 0 697 1133 1689
Size: 697 436 556
Fragments arranged by size:
697 556 436
MspA1I CmG'CkG
Cuts at: 0 661 1689
Size: 661 1028
NsiI A_TGCA'T
Cuts at: 0 1662 1689
Size: 1662 27
NspI r_CATG'y
Cuts at: 0 111 1689
Size: 111 1578
PasI CC'CwG_GG
Cuts at: 0 795 1689
Size: 795 894
PciI A'CATG_T
Cuts at: 0 107 1689
Size: 107 1582
PflMI CCAn_nnn'nTGG
Cuts at: 0 639 675 1689
Size: 639 36 1014
Fragments arranged by size:
1014 639 36
PfoI T'CCnGG_A
Cuts at: 0 284 1689
Size: 284 1405
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1110 1142 1689
Size: 1110 32 547
Fragments arranged by size:
1110 547 32
PpuMI rG'GwC_Cy
Cuts at: 0 426 1689
Size: 426 1263
PspOMI G'GGCC_C
Cuts at: 0 205 1689
Size: 205 1484
PspXI vC'TCGA_Gb
Cuts at: 0 498 1689
Size: 498 1191
PstI C_TGCA'G
Cuts at: 0 350 1689
Size: 350 1339
SacI G_AGCT'C
Cuts at: 0 335 582 1084 1420 1689
Size: 335 247 502 336 269
Fragments arranged by size:
502 336 335 269 247
SapI GCTCTTCn'nnn_
Cuts at: 0 587 1689
Size: 587 1102
SfcI C'TryA_G
Cuts at: 0 130 346 998 1689
Size: 130 216 652 691
Fragments arranged by size:
691 652 216 130
SmlI C'TyrA_G
Cuts at: 0 498 1535 1689
Size: 498 1037 154
Fragments arranged by size:
1037 498 154
StuI AGG'CCT
Cuts at: 0 1321 1405 1573 1689
Size: 1321 84 168 116
Fragments arranged by size:
1321 168 116 84
StyI C'CwwG_G
Cuts at: 0 272 326 1039 1689
Size: 272 54 713 650
Fragments arranged by size:
713 650 272 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 541 1689
Size: 541 1148
TatI w'GTAC_w
Cuts at: 0 1637 1689
Size: 1637 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 129 161 1689
Size: 129 32 1528
Fragments arranged by size:
1528 129 32
XbaI T'CTAG_A
Cuts at: 0 390 1689
Size: 390 1299
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 199 898 1689
Size: 199 699 791
Fragments arranged by size:
791 699 199
XhoI C'TCGA_G
Cuts at: 0 498 1535 1689
Size: 498 1037 154
Fragments arranged by size:
1037 498 154
XmnI GAAnn'nnTTC
Cuts at: 0 1106 1237 1405 1689
Size: 1106 131 168 284
Fragments arranged by size:
1106 284 168 131
Enzymes that do cut:
AcuI AflIII AhdI AleI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbsI BfrBI Bme1580I BmrI
BplI BpmI BsaI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BsrDI BssSI BstYI Bsu36I BtgI
BtgZI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI HaeII Hin4I HincII HindIII MmeI MslI
MspA1I NsiI NspI PasI PciI PflMI PfoI PpiI
PpuMI PspOMI PspXI PstI SacI SapI SfcI SmlI
StuI StyI TaqII TatI TstI XbaI XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AlfI AloI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BciVI BclI BglI BglII BlpI BmgBI
BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmI BspEI BspMI BsrBI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstZ17I BtsI ClaI CspCI
DraI DrdI EagI EcoRV FalI FseI FspI FspAI
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PmeI
PmlI PshAI PsiI PsrI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XmaI ZraI