Restriction map for
pF1KF0107
(Linear) (Six-base) MAPSORT of: FLJ00107.seq Check: 1896 from: 1 to: 3933
FLJ00107.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 894 1887 2144 3933
Size: 894 993 257 1789
Fragments arranged by size:
1789 993 894 257
AccI GT'mk_AC
Cuts at: 0 851 1427 3933
Size: 851 576 2506
Fragments arranged by size:
2506 851 576
AclI AA'CG_TT
Cuts at: 0 3431 3933
Size: 3431 502
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 1459 2059 2110 3427 3933
Size: 89 1370 600 51 1317 506
Fragments arranged by size:
1370 1317 600 506 89 51
AflII C'TTAA_G
Cuts at: 0 1366 1809 1849 3933
Size: 1366 443 40 2084
Fragments arranged by size:
2084 1366 443 40
AflIII A'CryG_T
Cuts at: 0 587 2798 2876 3933
Size: 587 2211 78 1057
Fragments arranged by size:
2211 1057 587 78
AleI CACnn'nnGTG
Cuts at: 0 143 527 2113 2383 3933
Size: 143 384 1586 270 1550
Fragments arranged by size:
1586 1550 384 270 143
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3092 3124 3933
Size: 3092 32 809
Fragments arranged by size:
3092 809 32
ApaLI G'TGCA_C
Cuts at: 0 136 1874 2259 3933
Size: 136 1738 385 1674
Fragments arranged by size:
1738 1674 385 136
ApoI r'AATT_y
Cuts at: 0 1698 2014 3042 3217 3933
Size: 1698 316 1028 175 716
Fragments arranged by size:
1698 1028 716 316 175
AseI AT'TA_AT
Cuts at: 0 200 3933
Size: 200 3733
AvaI C'yCGr_G
Cuts at: 0 1188 3933
Size: 1188 2745
AvrII C'CTAG_G
Cuts at: 0 2856 3933
Size: 2856 1077
BanI G'GyrC_C
Cuts at: 0 2377 3760 3933
Size: 2377 1383 173
Fragments arranged by size:
2377 1383 173
BanII G_rGCy'C
Cuts at: 0 866 2774 3491 3933
Size: 866 1908 717 442
Fragments arranged by size:
1908 866 717 442
BbsI GAAGACnn'nnnn_
Cuts at: 0 65 113 131 782 2151 2636 3415 3583
Size: 65 48 18 651 1369 485 779 168
Cuts at: 3583 3851 3933
Size: 268 82
Fragments arranged by size:
1369 779 651 485 268 168 82 65
48 18
BbvCI CC'TCA_GC
Cuts at: 0 388 3933
Size: 388 3545
BclI T'GATC_A
Cuts at: 0 558 2063 3933
Size: 558 1505 1870
Fragments arranged by size:
1870 1505 558
BfrBI ATG'CAT
Cuts at: 0 1534 3590 3933
Size: 1534 2056 343
Fragments arranged by size:
2056 1534 343
BglII A'GATC_T
Cuts at: 0 3234 3851 3933
Size: 3234 617 82
Fragments arranged by size:
3234 617 82
BlpI GC'TnA_GC
Cuts at: 0 175 2768 3933
Size: 175 2593 1165
Fragments arranged by size:
2593 1165 175
Bme1580I G_kGCm'C
Cuts at: 0 140 1878 2263 2884 3933
Size: 140 1738 385 621 1049
Fragments arranged by size:
1738 1049 621 385 140
BmgBI CAC'GTC
Cuts at: 0 590 1885 3180 3933
Size: 590 1295 1295 753
Fragments arranged by size:
1295 1295 753 590
BmrI ACTGGGnnnn_n'
Cuts at: 0 2283 3555 3933
Size: 2283 1272 378
Fragments arranged by size:
2283 1272 378
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 856 897 1175 3933
Size: 856 41 278 2758
Fragments arranged by size:
2758 856 278 41
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 826 1058 1622 1913 2759 2974 3442
Size: 142 684 232 564 291 846 215 468
Cuts at: 3442 3703 3933
Size: 261 230
Fragments arranged by size:
846 684 564 468 291 261 232 230
215 142
Bpu10I CC'TnA_GC
Cuts at: 0 388 1385 3933
Size: 388 997 2548
Fragments arranged by size:
2548 997 388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3057 3933
Size: 3057 876
BsaI GGTCTCn'nnnn_
Cuts at: 0 871 1640 2714 3933
Size: 871 769 1074 1219
Fragments arranged by size:
1219 1074 871 769
BsaWI w'CCGG_w
Cuts at: 0 697 1002 3933
Size: 697 305 2931
Fragments arranged by size:
2931 697 305
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 215 245 3933
Size: 215 30 3688
Fragments arranged by size:
3688 215 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 889 3016 3933
Size: 130 759 2127 917
Fragments arranged by size:
2127 917 759 130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 762 1865 2154 2572 3933
Size: 762 1103 289 418 1361
Fragments arranged by size:
1361 1103 762 418 289
BsiHKAI G_wGCw'C
Cuts at: 0 140 866 1878 2263 2774 2806 3491 3740
Size: 140 726 1012 385 511 32 685 249
Cuts at: 3740 3933
Size: 193
Fragments arranged by size:
1012 726 685 511 385 249 193 140
32
BsmI GAATG_Cn'
Cuts at: 0 817 1534 3682 3933
Size: 817 717 2148 251
Fragments arranged by size:
2148 817 717 251
Bsp1286I G_dGCh'C
Cuts at: 0 140 866 1878 2263 2774 2806 2884 3491
Size: 140 726 1012 385 511 32 78 607
Cuts at: 3491 3740 3933
Size: 249 193
Fragments arranged by size:
1012 726 607 511 385 249 193 140
78 32
BspHI T'CATG_A
Cuts at: 0 669 887 3933
Size: 669 218 3046
Fragments arranged by size:
3046 669 218
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 654 894 1887 2144 3933
Size: 654 240 993 257 1789
Fragments arranged by size:
1789 993 654 257 240
BsrBI CCG'CTC
Cuts at: 0 835 1159 2898 3933
Size: 835 324 1739 1035
Fragments arranged by size:
1739 1035 835 324
BsrDI GCAATG_nn'
Cuts at: 0 791 1548 2401 3015 3933
Size: 791 757 853 614 918
Fragments arranged by size:
918 853 791 757 614
BsrFI r'CCGG_y
Cuts at: 0 3280 3933
Size: 3280 653
BsrGI T'GTAC_A
Cuts at: 0 974 1693 3862 3933
Size: 974 719 2169 71
Fragments arranged by size:
2169 974 719 71
BssSI C'ACGA_G
Cuts at: 0 859 3490 3933
Size: 859 2631 443
Fragments arranged by size:
2631 859 443
BstAPI GCAn_nnn'nTGC
Cuts at: 0 449 1513 2177 2267 2360 2580 2801 3686
Size: 449 1064 664 90 93 220 221 885
Cuts at: 3686 3933
Size: 247
Fragments arranged by size:
1064 885 664 449 247 221 220 93
90
BstBI TT'CG_AA
Cuts at: 0 2504 3746 3933
Size: 2504 1242 187
Fragments arranged by size:
2504 1242 187
BstYI r'GATC_y
Cuts at: 0 28 569 3234 3851 3933
Size: 28 541 2665 617 82
Fragments arranged by size:
2665 617 541 82 28
BtgI C'CryG_G
Cuts at: 0 300 624 1106 3111 3834 3933
Size: 300 324 482 2005 723 99
Fragments arranged by size:
2005 723 482 324 300 99
BtsI GCAGTG_nn'
Cuts at: 0 522 534 3933
Size: 522 12 3399
Fragments arranged by size:
3399 522 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1091 1126 3933
Size: 1091 35 2807
Fragments arranged by size:
2807 1091 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 588 2200 3933
Size: 588 1612 1733
Fragments arranged by size:
1733 1612 588
EaeI y'GGCC_r
Cuts at: 0 256 1829 3933
Size: 256 1573 2104
Fragments arranged by size:
2104 1573 256
EarI CTCTTCn'nnn_
Cuts at: 0 91 871 1144 1478 1580 1581 2993 3933
Size: 91 780 273 334 102 1 1412 940
Fragments arranged by size:
1412 940 780 334 273 102 91 1
EcoICRI GAG'CTC
Cuts at: 0 864 2772 3489 3933
Size: 864 1908 717 444
Fragments arranged by size:
1908 864 717 444
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 142 826 1058 1459 1622 1913 2059
Size: 89 53 684 232 401 163 291 146
Cuts at: 2059 2110 2759 2974 3427 3442 3703 3933
Size: 51 649 215 453 15 261 230
Fragments arranged by size:
684 649 453 401 291 261 232 230
215 163 146 89 53 51 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2179 3933
Size: 2179 1754
EcoO109I rG'GnC_Cy
Cuts at: 0 1332 1389 1805 2860 3933
Size: 1332 57 416 1055 1073
Fragments arranged by size:
1332 1073 1055 416 57
EcoRI G'AATT_C
Cuts at: 0 2014 3042 3217 3933
Size: 2014 1028 175 716
Fragments arranged by size:
2014 1028 716 175
EcoRV GAT'ATC
Cuts at: 0 183 3252 3933
Size: 183 3069 681
Fragments arranged by size:
3069 681 183
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 591 3903 3933
Size: 591 3312 30
Fragments arranged by size:
3312 591 30
FspI TGC'GCA
Cuts at: 0 3201 3933
Size: 3201 732
FspAI rTGC'GCAy
Cuts at: 0 3201 3933
Size: 3201 732
HaeII r_GCGC'y
Cuts at: 0 3308 3933
Size: 3308 625
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1828 1860 1886 1918 1983 2015 3933
Size: 1828 32 26 32 65 32 1918
Fragments arranged by size:
1918 1828 65 32 32 32 26
HincII GTy'rAC
Cuts at: 0 211 1458 2637 3933
Size: 211 1247 1179 1296
Fragments arranged by size:
1296 1247 1179 211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 268 617 1106 3545 3933
Size: 101 167 349 489 2439 388
Fragments arranged by size:
2439 489 388 349 167 101
MscI TGG'CCA
Cuts at: 0 258 1831 3933
Size: 258 1573 2102
Fragments arranged by size:
2102 1573 258
MslI CAynn'nnrTG
Cuts at: 0 143 354 527 606 2113 2383 3933
Size: 143 211 173 79 1507 270 1550
Fragments arranged by size:
1550 1507 270 211 173 143 79
MspA1I CmG'CkG
Cuts at: 0 143 152 1217 3933
Size: 143 9 1065 2716
Fragments arranged by size:
2716 1065 143 9
NaeI GCC'GGC
Cuts at: 0 3282 3933
Size: 3282 651
NcoI C'CATG_G
Cuts at: 0 300 1106 3111 3834 3933
Size: 300 806 2005 723 99
Fragments arranged by size:
2005 806 723 300 99
NdeI CA'TA_TG
Cuts at: 0 2843 3267 3933
Size: 2843 424 666
Fragments arranged by size:
2843 666 424
NgoMIV G'CCGG_C
Cuts at: 0 3280 3933
Size: 3280 653
NsiI A_TGCA'T
Cuts at: 0 1536 3592 3933
Size: 1536 2056 341
Fragments arranged by size:
2056 1536 341
NspI r_CATG'y
Cuts at: 0 663 2047 2148 2880 3078 3129 3933
Size: 663 1384 101 732 198 51 804
Fragments arranged by size:
1384 804 732 663 198 101 51
PasI CC'CwG_GG
Cuts at: 0 340 3933
Size: 340 3593
PciI A'CATG_T
Cuts at: 0 2876 3933
Size: 2876 1057
PflMI CCAn_nnn'nTGG
Cuts at: 0 1645 3933
Size: 1645 2288
PfoI T'CCnGG_A
Cuts at: 0 703 770 2834 3933
Size: 703 67 2064 1099
Fragments arranged by size:
2064 1099 703 67
PpuMI rG'GwC_Cy
Cuts at: 0 1332 1389 3933
Size: 1332 57 2544
Fragments arranged by size:
2544 1332 57
PshAI GACnn'nnGTC
Cuts at: 0 1394 3933
Size: 1394 2539
PspXI vC'TCGA_Gb
Cuts at: 0 1188 3933
Size: 1188 2745
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1428 1460 3933
Size: 1428 32 2473
Fragments arranged by size:
2473 1428 32
PstI C_TGCA'G
Cuts at: 0 454 529 1265 1518 2576 2705 3153 3691
Size: 454 75 736 253 1058 129 448 538
Cuts at: 3691 3905 3933
Size: 214 28
Fragments arranged by size:
1058 736 538 454 448 253 214 129
75 28
PvuII CAG'CTG
Cuts at: 0 143 152 1217 3933
Size: 143 9 1065 2716
Fragments arranged by size:
2716 1065 143 9
SacI G_AGCT'C
Cuts at: 0 866 2774 3491 3933
Size: 866 1908 717 442
Fragments arranged by size:
1908 866 717 442
SapI GCTCTTCn'nnn_
Cuts at: 0 871 1580 3933
Size: 871 709 2353
Fragments arranged by size:
2353 871 709
SfcI C'TryA_G
Cuts at: 0 450 525 1212 1261 1514 2253 2439 2572
Size: 450 75 687 49 253 739 186 133
Cuts at: 2572 2701 3149 3687 3901 3933
Size: 129 448 538 214 32
Fragments arranged by size:
739 687 538 450 448 253 214 186
133 129 75 49 32
SmlI C'TyrA_G
Cuts at: 0 1188 1366 1809 1849 3036 3933
Size: 1188 178 443 40 1187 897
Fragments arranged by size:
1188 1187 897 443 178 40
SphI G_CATG'C
Cuts at: 0 3078 3933
Size: 3078 855
SspI AAT'ATT
Cuts at: 0 1709 3933
Size: 1709 2224
StyI C'CwwG_G
Cuts at: 0 300 1106 1766 2334 2856 3095 3111 3834
Size: 300 806 660 568 522 239 16 723
Cuts at: 3834 3933
Size: 99
Fragments arranged by size:
806 723 660 568 522 300 239 99
16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 845 2532 3933
Size: 845 1687 1401
Fragments arranged by size:
1687 1401 845
TatI w'GTAC_w
Cuts at: 0 974 1693 1925 2423 3862 3933
Size: 974 719 232 498 1439 71
Fragments arranged by size:
1439 974 719 498 232 71
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1965 1997 3933
Size: 1965 32 1936
Fragments arranged by size:
1965 1936 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1089 3234 3933
Size: 1089 2145 699
Fragments arranged by size:
2145 1089 699
XhoI C'TCGA_G
Cuts at: 0 1188 3933
Size: 1188 2745
XmnI GAAnn'nnTTC
Cuts at: 0 3883 3933
Size: 3883 50
Enzymes that do cut:
AarI AccI AclI AcuI AflII AflIII AleI AloI
ApaLI ApoI AseI AvaI AvrII BanI BanII BbsI
BbvCI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtsI
CspCI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PpuMI PshAI
PspXI PsrI PstI PvuII SacI SapI SfcI SmlI
SphI SspI StyI TaqII TatI TstI XcmI XhoI
XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AgeI AhdI AlfI AlwNI ApaI
AscI AsiSI BaeI BamHI BbeI BcgI BciVI BglI
BmtI BsaAI BsaBI BsaHI BseYI BsiEI BsiWI BsmBI
BspEI BssHII BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
DraI DraIII EagI EciI FseI HindIII HpaI KasI
KpnI MfeI MluI NarI NheI NotI NruI PacI
PmeI PmlI PpiI PsiI PspOMI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI StuI SwaI Tth111I
XbaI XmaI ZraI