Restriction map for
pF1KF0034
(Linear) (Six-base) MAPSORT of: FLJ00034.seq Check: 264 from: 1 to: 3936
FLJ00034.orf
With 182 enzymes: SgfI *
March 3, 2008 11:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 669 1368 3936
Size: 669 699 2568
Fragments arranged by size:
2568 699 669
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2845 3643 3936
Size: 2845 798 293
Fragments arranged by size:
2845 798 293
AfeI AGC'GCT
Cuts at: 0 1477 1489 3817 3936
Size: 1477 12 2328 119
Fragments arranged by size:
2328 1477 119 12
AhdI GACnn_n'nnGTC
Cuts at: 0 1338 3936
Size: 1338 2598
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3140 3814 3936
Size: 3140 674 122
Fragments arranged by size:
3140 674 122
AlwNI CAG_nnn'CTG
Cuts at: 0 458 2993 3378 3448 3936
Size: 458 2535 385 70 488
Fragments arranged by size:
2535 488 458 385 70
ApaI G_GGCC'C
Cuts at: 0 550 1320 2420 2649 3869 3896 3936
Size: 550 770 1100 229 1220 27 40
Fragments arranged by size:
1220 1100 770 550 229 40 27
ApoI r'AATT_y
Cuts at: 0 2593 3936
Size: 2593 1343
AvaI C'yCGr_G
Cuts at: 0 20 1025 1321 1805 1872 1946 2144 2259
Size: 20 1005 296 484 67 74 198 115
Cuts at: 2259 2288 3022 3201 3912 3936
Size: 29 734 179 711 24
Fragments arranged by size:
1005 734 711 484 296 198 179 115
74 67 29 24 20
AvrII C'CTAG_G
Cuts at: 0 1386 3936
Size: 1386 2550
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 314 347 3936
Size: 314 33 3589
Fragments arranged by size:
3589 314 33
BamHI G'GATC_C
Cuts at: 0 312 3936
Size: 312 3624
BanI G'GyrC_C
Cuts at: 0 1369 1503 1612 1886 1989 2252 2403 2863
Size: 1369 134 109 274 103 263 151 460
Cuts at: 2863 3936
Size: 1073
Fragments arranged by size:
1369 1073 460 274 263 151 134 109
103
BanII G_rGCy'C
Cuts at: 0 224 550 758 958 1208 1320 2420 2581
Size: 224 326 208 200 250 112 1100 161
Cuts at: 2581 2649 3869 3896 3936
Size: 68 1220 27 40
Fragments arranged by size:
1220 1100 326 250 224 208 200 161
112 68 40 27
BbeI G_GCGC'C
Cuts at: 0 1373 1507 1993 2256 2407 3936
Size: 1373 134 486 263 151 1529
Fragments arranged by size:
1529 1373 486 263 151 134
BbsI GAAGACnn'nnnn_
Cuts at: 0 34 851 1047 3936
Size: 34 817 196 2889
Fragments arranged by size:
2889 817 196 34
BbvCI CC'TCA_GC
Cuts at: 0 389 1298 3403 3936
Size: 389 909 2105 533
Fragments arranged by size:
2105 909 533 389
BciVI GTATCCnnnnn_n'
Cuts at: 0 315 3936
Size: 315 3621
BglI GCCn_nnn'nGGC
Cuts at: 0 1238 1270 1591 1784 3352 3873 3936
Size: 1238 32 321 193 1568 521 63
Fragments arranged by size:
1568 1238 521 321 193 63 32
BglII A'GATC_T
Cuts at: 0 3746 3936
Size: 3746 190
Bme1580I G_kGCm'C
Cuts at: 0 247 434 516 535 550 1320 1615 2420
Size: 247 187 82 19 15 770 295 805
Cuts at: 2420 2649 3455 3869 3896 3936
Size: 229 806 414 27 40
Fragments arranged by size:
806 805 770 414 295 247 229 187
82 40 27 19 15
BmgBI CAC'GTC
Cuts at: 0 3563 3936
Size: 3563 373
BmrI ACTGGGnnnn_n'
Cuts at: 0 3772 3936
Size: 3772 164
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 729 1150 1614 2634 3936
Size: 729 421 464 1020 1302
Fragments arranged by size:
1302 1020 729 464 421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 480 3468 3936
Size: 397 83 2988 468
Fragments arranged by size:
2988 468 397 83
Bpu10I CC'TnA_GC
Cuts at: 0 389 928 1298 2177 2448 3403 3936
Size: 389 539 370 879 271 955 533
Fragments arranged by size:
955 879 539 533 389 370 271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3408 3936
Size: 3408 528
BsaI GGTCTCn'nnnn_
Cuts at: 0 68 889 1104 2306 3015 3936
Size: 68 821 215 1202 709 921
Fragments arranged by size:
1202 921 821 709 215 68
BsaBI GATnn'nnATC
Cuts at: 0 3617 3936
Size: 3617 319
BsaHI Gr'CG_yC
Cuts at: 0 1370 1504 1990 2253 2404 2588 3305 3936
Size: 1370 134 486 263 151 184 717 631
Fragments arranged by size:
1370 717 631 486 263 184 151 134
BsaWI w'CCGG_w
Cuts at: 0 729 2967 3936
Size: 729 2238 969
Fragments arranged by size:
2238 969 729
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 72 102 465 495 724 754 2176 2206
Size: 72 30 363 30 229 30 1422 30
Cuts at: 2206 2284 2314 3429 3459 3936
Size: 78 30 1115 30 477
Fragments arranged by size:
1422 1115 477 363 229 78 72 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 486 561 573 817 826 829 865
Size: 49 437 75 12 244 9 3 36
Cuts at: 865 868 871 1213 1216 2160 2233 2262 2265
Size: 3 3 342 3 944 73 29 3
Cuts at: 2265 2268 2271 2893 2914 3040 3052 3079 3082
Size: 3 3 622 21 126 12 27 3
Cuts at: 3082 3085 3088 3103 3106 3121 3124 3127 3670
Size: 3 3 15 3 15 3 3 543
Cuts at: 3670 3724 3936
Size: 54 212
Fragments arranged by size:
944 622 543 437 342 244 212 126
75 73 54 49 36 29 27 21
15 15 12 12 9 3 3 3
3 3 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 334 433 651 772 1286 1427 1616 3043
Size: 334 99 218 121 514 141 189 1427
Cuts at: 3043 3175 3236 3441 3936
Size: 132 61 205 495
Fragments arranged by size:
1427 514 495 334 218 205 189 141
132 121 99 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 3276 3305 3616 3936
Size: 629 2647 29 311 320
Fragments arranged by size:
2647 629 320 311 29
BsiEI CG_ry'CG
Cuts at: 0 1242 1265 1523 1773 1939 3843 3877 3936
Size: 1242 23 258 250 166 1904 34 59
Fragments arranged by size:
1904 1242 258 250 166 59 34 23
BsmBI CGTCTCn'nnnn_
Cuts at: 0 454 533 3936
Size: 454 79 3403
Fragments arranged by size:
3403 454 79
Bsp1286I G_dGCh'C
Cuts at: 0 224 247 434 516 535 550 758 958
Size: 224 23 187 82 19 15 208 200
Cuts at: 958 1208 1320 1615 2420 2581 2649 3455 3869
Size: 250 112 295 805 161 68 806 414
Cuts at: 3869 3896 3936
Size: 27 40
Fragments arranged by size:
806 805 414 295 250 224 208 200
187 161 112 82 68 40 27 23
19 15
BspEI T'CCGG_A
Cuts at: 0 729 2967 3936
Size: 729 2238 969
Fragments arranged by size:
2238 969 729
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 158 356 669 1368 1451 1784 3500 3936
Size: 158 198 313 699 83 333 1716 436
Fragments arranged by size:
1716 699 436 333 313 198 158 83
BsrBI CCG'CTC
Cuts at: 0 1309 1667 1718 1724 1730 1736 1742 1757
Size: 1309 358 51 6 6 6 6 15
Cuts at: 1757 1884 2213 2223 2258 2643 2904 3645 3936
Size: 127 329 10 35 385 261 741 291
Fragments arranged by size:
1309 741 385 358 329 291 261 127
51 35 15 10 6 6 6 6
BsrFI r'CCGG_y
Cuts at: 0 760 1046 1260 1395 2123 2569 3193 3936
Size: 760 286 214 135 728 446 624 743
Fragments arranged by size:
760 743 728 624 446 286 214 135
BssSI C'ACGA_G
Cuts at: 0 2019 3936
Size: 2019 1917
BstEII G'GTnAC_C
Cuts at: 0 1113 2026 2326 3936
Size: 1113 913 300 1610
Fragments arranged by size:
1610 1113 913 300
BstXI CCAn_nnnn'nTGG
Cuts at: 0 342 2118 2658 3462 3552 3936
Size: 342 1776 540 804 90 384
Fragments arranged by size:
1776 804 540 384 342 90
BstYI r'GATC_y
Cuts at: 0 312 411 1454 3398 3746 3936
Size: 312 99 1043 1944 348 190
Fragments arranged by size:
1944 1043 348 312 190 99
Bsu36I CC'TnA_GG
Cuts at: 0 800 2412 3005 3727 3936
Size: 800 1612 593 722 209
Fragments arranged by size:
1612 800 722 593 209
BtgI C'CryG_G
Cuts at: 0 211 275 1280 1312 1779 1890 2981 3134
Size: 211 64 1005 32 467 111 1091 153
Cuts at: 3134 3600 3871 3936
Size: 466 271 65
Fragments arranged by size:
1091 1005 467 466 271 211 153 111
65 64 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 71 3936
Size: 71 3865
BtsI GCAGTG_nn'
Cuts at: 0 2790 2811 3146 3167 3936
Size: 2790 21 335 21 769
Fragments arranged by size:
2790 769 335 21 21
DraIII CAC_nnn'GTG
Cuts at: 0 2021 3936
Size: 2021 1915
DrdI GACnn_nn'nnGTC
Cuts at: 0 36 2889 3936
Size: 36 2853 1047
Fragments arranged by size:
2853 1047 36
EaeI y'GGCC_r
Cuts at: 0 272 1239 1262 1271 1520 1850 2549 2660
Size: 272 967 23 9 249 330 699 111
Cuts at: 2660 2811 3137 3668 3874 3936
Size: 151 326 531 206 62
Fragments arranged by size:
967 699 531 330 326 272 249 206
151 111 62 23 9
EagI C'GGCC_G
Cuts at: 0 1239 1262 1520 3874 3936
Size: 1239 23 258 2354 62
Fragments arranged by size:
2354 1239 258 62 23
EarI CTCTTCn'nnn_
Cuts at: 0 803 815 827 833 1231 2098 2289 2460
Size: 803 12 12 6 398 867 191 171
Cuts at: 2460 3080 3092 3098 3334 3936
Size: 620 12 6 236 602
Fragments arranged by size:
867 803 620 602 398 236 191 171
12 12 12 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 53 1219 1526 1695 1824 2854 2890 3076
Size: 53 1166 307 169 129 1030 36 186
Cuts at: 3076 3223 3936
Size: 147 713
Fragments arranged by size:
1166 1030 713 307 186 169 147 129
53 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 480 2845 3468 3643 3936
Size: 397 83 2365 623 175 293
Fragments arranged by size:
2365 623 397 293 175 83
EcoNI CCTnn'n_nnAGG
Cuts at: 0 931 2411 3022 3730 3936
Size: 931 1480 611 708 206
Fragments arranged by size:
1480 931 708 611 206
EcoO109I rG'GnC_Cy
Cuts at: 0 147 455 546 547 788 1317 1983 2073
Size: 147 308 91 1 241 529 666 90
Cuts at: 2073 2149 2187 2416 2417 2646 3855 3865 3866
Size: 76 38 229 1 229 1209 10 1
Cuts at: 3866 3892 3893 3936
Size: 26 1 43
Fragments arranged by size:
1209 666 529 308 241 229 229 147
91 90 76 43 38 26 10 1
1 1 1
EcoRV GAT'ATC
Cuts at: 0 678 3936
Size: 678 3258
HaeII r_GCGC'y
Cuts at: 0 201 1219 1373 1479 1491 1507 1627 1833
Size: 201 1018 154 106 12 16 120 206
Cuts at: 1833 1993 2256 2317 2407 3819 3936
Size: 160 263 61 90 1412 117
Fragments arranged by size:
1412 1018 263 206 201 160 154 120
117 106 90 61 16 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 401 433 662 694 1324 1356 1945 1977
Size: 401 32 229 32 630 32 589 32
Cuts at: 1977 2284 2316 2356 2388 2548 2580 3429 3461
Size: 307 32 40 32 160 32 849 32
Cuts at: 3461 3936
Size: 475
Fragments arranged by size:
849 630 589 475 401 307 229 160
40 32 32 32 32 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 1369 1503 1989 2252 2403 3936
Size: 1369 134 486 263 151 1533
Fragments arranged by size:
1533 1369 486 263 151 134
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 791 1337 2162 2287 3936
Size: 245 546 546 825 125 1649
Fragments arranged by size:
1649 825 546 546 245 125
MscI TGG'CCA
Cuts at: 0 274 2662 2813 3670 3936
Size: 274 2388 151 857 266
Fragments arranged by size:
2388 857 274 266 151
MslI CAynn'nnrTG
Cuts at: 0 2116 2656 3936
Size: 2116 540 1280
Fragments arranged by size:
2116 1280 540
MspA1I CmG'CkG
Cuts at: 0 784 1120 1314 1528 1577 1781 1892 2444
Size: 784 336 194 214 49 204 111 552
Cuts at: 2444 2798 2993 3240 3280 3302 3936
Size: 354 195 247 40 22 634
Fragments arranged by size:
784 634 552 354 336 247 214 204
195 194 111 49 40 22
NaeI GCC'GGC
Cuts at: 0 1048 1397 2125 2571 3936
Size: 1048 349 728 446 1365
Fragments arranged by size:
1365 1048 728 446 349
NarI GG'CG_CC
Cuts at: 0 1370 1504 1990 2253 2404 3936
Size: 1370 134 486 263 151 1532
Fragments arranged by size:
1532 1370 486 263 151 134
NgoMIV G'CCGG_C
Cuts at: 0 1046 1395 2123 2569 3936
Size: 1046 349 728 446 1367
Fragments arranged by size:
1367 1046 728 446 349
NspI r_CATG'y
Cuts at: 0 375 1351 3936
Size: 375 976 2585
Fragments arranged by size:
2585 976 375
PasI CC'CwG_GG
Cuts at: 0 1646 3862 3936
Size: 1646 2216 74
Fragments arranged by size:
2216 1646 74
PflMI CCAn_nnn'nTGG
Cuts at: 0 3266 3936
Size: 3266 670
PfoI T'CCnGG_A
Cuts at: 0 308 2069 2482 3936
Size: 308 1761 413 1454
Fragments arranged by size:
1761 1454 413 308
PpuMI rG'GwC_Cy
Cuts at: 0 147 788 2073 2149 2187 3855 3936
Size: 147 641 1285 76 38 1668 81
Fragments arranged by size:
1668 1285 641 147 81 76 38
PshAI GACnn'nnGTC
Cuts at: 0 2701 3936
Size: 2701 1235
PspOMI G'GGCC_C
Cuts at: 0 546 1316 2416 2645 3865 3892 3936
Size: 546 770 1100 229 1220 27 44
Fragments arranged by size:
1220 1100 770 546 229 44 27
PspXI vC'TCGA_Gb
Cuts at: 0 1872 3936
Size: 1872 2064
PstI C_TGCA'G
Cuts at: 0 143 1379 2501 2851 3156 3936
Size: 143 1236 1122 350 305 780
Fragments arranged by size:
1236 1122 780 350 305 143
PvuII CAG'CTG
Cuts at: 0 2993 3240 3280 3936
Size: 2993 247 40 656
Fragments arranged by size:
2993 656 247 40
SacII CC_GC'GG
Cuts at: 0 1315 1782 1893 3936
Size: 1315 467 111 2043
Fragments arranged by size:
2043 1315 467 111
SanDI GG'GwC_CC
Cuts at: 0 788 3855 3936
Size: 788 3067 81
Fragments arranged by size:
3067 788 81
SapI GCTCTTCn'nnn_
Cuts at: 0 2460 3936
Size: 2460 1476
SbfI CC_TGCA'GG
Cuts at: 0 143 1379 3936
Size: 143 1236 2557
Fragments arranged by size:
2557 1236 143
SexAI A'CCwGG_T
Cuts at: 0 3794 3936
Size: 3794 142
SfcI C'TryA_G
Cuts at: 0 139 1375 2497 2847 3152 3936
Size: 139 1236 1122 350 305 784
Fragments arranged by size:
1236 1122 784 350 305 139
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1270 1591 3873 3936
Size: 1270 321 2282 63
Fragments arranged by size:
2282 1270 321 63
SfoI GGC'GCC
Cuts at: 0 1371 1505 1991 2254 2405 3936
Size: 1371 134 486 263 151 1531
Fragments arranged by size:
1531 1371 486 263 151 134
SgrAI Cr'CCGG_yG
Cuts at: 0 1046 3936
Size: 1046 2890
SmaI CCC'GGG
Cuts at: 0 1027 1807 1948 2290 3914 3936
Size: 1027 780 141 342 1624 22
Fragments arranged by size:
1624 1027 780 342 141 22
SmlI C'TyrA_G
Cuts at: 0 20 1872 3423 3936
Size: 20 1852 1551 513
Fragments arranged by size:
1852 1551 513 20
SphI G_CATG'C
Cuts at: 0 1351 3936
Size: 1351 2585
SrfI GCCC'GGGC
Cuts at: 0 3914 3936
Size: 3914 22
StuI AGG'CCT
Cuts at: 0 709 866 3936
Size: 709 157 3070
Fragments arranged by size:
3070 709 157
StyI C'CwwG_G
Cuts at: 0 527 1386 1586 2393 2504 2552 2699 2711
Size: 527 859 200 807 111 48 147 12
Cuts at: 2711 2732 2741 2834 2867 2912 2936 3704 3881
Size: 21 9 93 33 45 24 768 177
Cuts at: 3881 3936
Size: 55
Fragments arranged by size:
859 807 768 527 200 177 147 111
93 55 48 45 33 24 21 12
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1084 1630 3936
Size: 1084 546 2306
Fragments arranged by size:
2306 1084 546
TatI w'GTAC_w
Cuts at: 0 3713 3936
Size: 3713 223
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 72 104 2051 2083 3936
Size: 72 32 1947 32 1853
Fragments arranged by size:
1947 1853 72 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3306 3760 3936
Size: 3306 454 176
Fragments arranged by size:
3306 454 176
XhoI C'TCGA_G
Cuts at: 0 20 1872 3936
Size: 20 1852 2064
Fragments arranged by size:
2064 1852 20
XmaI C'CCGG_G
Cuts at: 0 1025 1805 1946 2288 3912 3936
Size: 1025 780 141 342 1624 24
Fragments arranged by size:
1624 1025 780 342 141 24
Enzymes that do cut:
AarI AcuI AfeI AhdI AlfI AlwNI ApaI ApoI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BglI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrFI BssSI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I KasI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PflMI PfoI PpuMI PshAI PspOMI PspXI PstI PvuII
SacII SanDI SapI SbfI SexAI SfcI SfiI SfoI
SgrAI SmaI SmlI SphI SrfI StuI StyI TaqII
TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AleI
AloI ApaLI AscI AseI AsiSI BcgI BclI BfrBI
BlpI BmtI BsaAI BsiHKAI BsiWI BsmI BspHI BsrDI
BsrGI BssHII BstAPI BstBI BstZ17I ClaI CspCI DraI
EcoICRI EcoRI FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PsiI
PsrI PvuI RsrII SacI SalI ScaI SgfI SnaBI
SpeI SspI SwaI Tth111I XbaI XmnI ZraI