Restriction map for pF1KF0006
 (Linear) (Six-base) MAPSORT of: FLJ00006.seq  Check: 7233  from: 1  to: 2247



FLJ00006.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      27    1983    2247 
   Size:         27    1956     264

  Fragments arranged by size: 

                1956     264      27
 
Acc65I G'GTAC_C

Cuts at:      0    2051    2247 
   Size:       2051     196
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     693    1747    1833    2115    2206    2211    2247 
   Size:        693    1054      86     282      91       5      36

  Fragments arranged by size: 

                1054     693     282      91      86      36       5
 
AflII C'TTAA_G

Cuts at:      0    1702    2247 
   Size:       1702     545
 
AleI CACnn'nnGTG

Cuts at:      0     366    1087    1525    2247 
   Size:        366     721     438     722

  Fragments arranged by size: 

                 722     721     438     366
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     556     736    2247 
   Size:        556     180    1511

  Fragments arranged by size: 

                1511     556     180
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     976    1008    2247 
   Size:        976      32    1239

  Fragments arranged by size: 

                1239     976      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     222     774    1152    1998    2184    2247 
   Size:        222     552     378     846     186      63

  Fragments arranged by size: 

                 846     552     378     222     186      63
 
ApaI G_GGCC'C

Cuts at:      0     390     570    2247 
   Size:        390     180    1677

  Fragments arranged by size: 

                1677     390     180
 
ApaLI G'TGCA_C

Cuts at:      0    1341    2247 
   Size:       1341     906
 
AvaI C'yCGr_G

Cuts at:      0     917    2079    2247 
   Size:        917    1162     168

  Fragments arranged by size: 

                1162     917     168
 
BanI G'GyrC_C

Cuts at:      0     540     607    1162    1445    1750    2051    2247 
   Size:        540      67     555     283     305     301     196

  Fragments arranged by size: 

                 555     540     305     301     283     196      67
 
BanII G_rGCy'C

Cuts at:      0     390     570     916    1565    1985    2108    2156    2247 
   Size:        390     180     346     649     420     123      48      91

  Fragments arranged by size: 

                 649     420     390     346     180     123      91      48
            
 
BbeI G_GCGC'C

Cuts at:      0    1449    1754    2247 
   Size:       1449     305     493

  Fragments arranged by size: 

                1449     493     305
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1765    1799    2099    2133    2247 
   Size:       1765      34     300      34     114

  Fragments arranged by size: 

                1765     300     114      34      34
 
BclI T'GATC_A

Cuts at:      0     824    2247 
   Size:        824    1423
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1577    1705    2247 
   Size:       1577     128     542

  Fragments arranged by size: 

                1577     542     128
 
BglII A'GATC_T

Cuts at:      0      68    2247 
   Size:         68    2179
 
Bme1580I G_kGCm'C

Cuts at:      0     390     545     570     610    1345    2247 
   Size:        390     155      25      40     735     902

  Fragments arranged by size: 

                 902     735     390     155      40      25
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2157    2247 
   Size:       2157      90
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     189    1705    2247 
   Size:        189    1516     542

  Fragments arranged by size: 

                1516     542     189
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     159     262     664     781    1273    1361    2010    2032 
   Size:        159     103     402     117     492      88     649      22

Cuts at:   2032    2247 
   Size:        215

  Fragments arranged by size: 

                 649     492     402     215     159     117     103      88
                  22
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     940    1246    2247 
   Size:        940     306    1001

  Fragments arranged by size: 

                1001     940     306
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     448    2247 
   Size:        448    1799
 
BsaAI yAC'GTr

Cuts at:      0    1422    2247 
   Size:       1422     825
 
BsaHI Gr'CG_yC

Cuts at:      0     656    1446    1751    2195    2247 
   Size:        656     790     305     444      52

  Fragments arranged by size: 

                 790     656     444     305      52
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     898     928     976    1006    1798    1828    2035    2065 
   Size:        898      30      48      30     792      30     207      30

Cuts at:   2065    2247 
   Size:        182

  Fragments arranged by size: 

                 898     792     207     182      48      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     661     664    1792    1900    2247 
   Size:        661       3    1128     108     347

  Fragments arranged by size: 

                1128     661     347     108       3
 
BseYI C'CCAG_C

Cuts at:      0     502     723     939    1202    1494    1550    2084    2231 
   Size:        502     221     216     263     292      56     534     147

Cuts at:   2231    2247 
   Size:         16

  Fragments arranged by size: 

                 534     502     292     263     221     216     147      56
                  16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     817     934    1927    1984    2247 
   Size:        817     117     993      57     263

  Fragments arranged by size: 

                 993     817     263     117      57
 
BsiHKAI G_wGCw'C

Cuts at:      0     563    1345    1432    1547    1565    1838    2021    2108 
   Size:        563     782      87     115      18     273     183      87

Cuts at:   2108    2156    2247 
   Size:         48      91

  Fragments arranged by size: 

                 782     563     273     183     115      91      87      87
                  48      18
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     300    2247 
   Size:        300    1947
 
Bsp1286I G_dGCh'C

Cuts at:      0     390     545     563     570     610     916    1345    1432 
   Size:        390     155      18       7      40     306     429      87

Cuts at:   1432    1547    1565    1838    1985    2021    2108    2156    2247 
   Size:        115      18     273     147      36      87      48      91

  Fragments arranged by size: 

                 429     390     306     273     155     147     115      91
                  87      87      48      40      36      18      18       7
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      27     494     698     782    1983    2247 
   Size:         27     467     204      84    1201     264

  Fragments arranged by size: 

                1201     467     264     204      84      27
 
BsrBI CCG'CTC

Cuts at:      0     308     323    1476    1964    2247 
   Size:        308      15    1153     488     283

  Fragments arranged by size: 

                1153     488     308     283      15
 
BsrDI GCAATG_nn'

Cuts at:      0    1130    2247 
   Size:       1130    1117
 
BsrFI r'CCGG_y

Cuts at:      0     337     610    1072    1159    1442    2247 
   Size:        337     273     462      87     283     805

  Fragments arranged by size: 

                 805     462     337     283     273      87
 
BsrGI T'GTAC_A

Cuts at:      0     146    2247 
   Size:        146    2101
 
BssSI C'ACGA_G

Cuts at:      0    1411    1675    2247 
   Size:       1411     264     572

  Fragments arranged by size: 

                1411     572     264
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     512    1939    1998    2247 
   Size:        512    1427      59     249

  Fragments arranged by size: 

                1427     512     249      59
 
BstBI TT'CG_AA

Cuts at:      0    1715    2247 
   Size:       1715     532
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     183     368     723     864    1068    1599    2247 
   Size:        183     185     355     141     204     531     648

  Fragments arranged by size: 

                 648     531     355     204     185     183     141
 
BstYI r'GATC_y

Cuts at:      0      68     270    2247 
   Size:         68     202    1977

  Fragments arranged by size: 

                1977     202      68
 
BtgI C'CryG_G

Cuts at:      0    1094    2039    2247 
   Size:       1094     945     208

  Fragments arranged by size: 

                1094     945     208
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     444    1620    2247 
   Size:        444    1176     627

  Fragments arranged by size: 

                1176     627     444
 
BtsI GCAGTG_nn'

Cuts at:      0     829    1223    1545    1940    2247 
   Size:        829     394     322     395     307

  Fragments arranged by size: 

                 829     395     394     322     307
 
DraIII CAC_nnn'GTG

Cuts at:      0     264    2247 
   Size:        264    1983
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     745    2247 
   Size:        745    1502
 
EaeI y'GGCC_r

Cuts at:      0      74     335     620    1058    1070    1091    2247 
   Size:         74     261     285     438      12      21    1156

  Fragments arranged by size: 

                1156     438     285     261      74      21      12
 
EarI CTCTTCn'nnn_

Cuts at:      0      89     394     959    1240    2158    2230    2247 
   Size:         89     305     565     281     918      72      17

  Fragments arranged by size: 

                 918     565     305     281      89      72      17
 
EcoICRI GAG'CTC

Cuts at:      0    1563    2106    2154    2247 
   Size:       1563     543      48      93

  Fragments arranged by size: 

                1563     543      93      48
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     159     262     664     693     781    1273    1361    1747 
   Size:        159     103     402      29      88     492      88     386

Cuts at:   1747    1833    2010    2032    2115    2206    2211    2247 
   Size:         86     177      22      83      91       5      36

  Fragments arranged by size: 

                 492     402     386     177     159     103      91      88
                  88      86      83      36      29      22       5
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     646     958    1180    2140    2188    2247 
   Size:        646     312     222     960      48      59

  Fragments arranged by size: 

                 960     646     312     222      59      48
 
EcoO109I rG'GnC_Cy

Cuts at:      0     386     387     483     567     687     747     894    1199 
   Size:        386       1      96      84     120      60     147     305

Cuts at:   1199    1458    1685    1707    2247 
   Size:        259     227      22     540

  Fragments arranged by size: 

                 540     386     305     259     227     147     120      96
                  84      60      22       1
 
EcoRV GAT'ATC

Cuts at:      0    1017    2247 
   Size:       1017    1230
 
FspI TGC'GCA

Cuts at:      0     553     886    2074    2247 
   Size:        553     333    1188     173

  Fragments arranged by size: 

                1188     553     333     173
 
HaeII r_GCGC'y

Cuts at:      0    1110    1449    1754    2247 
   Size:       1110     339     305     493

  Fragments arranged by size: 

                1110     493     339     305
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1006    1038    1798    1830    2247 
   Size:       1006      32     760      32     417

  Fragments arranged by size: 

                1006     760     417      32      32
 
KasI G'GCGC_C

Cuts at:      0    1445    1750    2247 
   Size:       1445     305     497

  Fragments arranged by size: 

                1445     497     305
 
KpnI G_GTAC'C

Cuts at:      0    2055    2247 
   Size:       2055     192
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     470    1835    2247 
   Size:        470    1365     412

  Fragments arranged by size: 

                1365     470     412
 
MscI TGG'CCA

Cuts at:      0      76    1060    2247 
   Size:         76     984    1187

  Fragments arranged by size: 

                1187     984      76
 
MslI CAynn'nnrTG

Cuts at:      0     366     538     613    1087    1525    2209    2247 
   Size:        366     172      75     474     438     684      38

  Fragments arranged by size: 

                 684     474     438     366     172      75      38
 
MspA1I CmG'CkG

Cuts at:      0     366     744    1206    1404    2231    2247 
   Size:        366     378     462     198     827      16

  Fragments arranged by size: 

                 827     462     378     366     198      16
 
NaeI GCC'GGC

Cuts at:      0     339    1074    1161    2247 
   Size:        339     735      87    1086

  Fragments arranged by size: 

                1086     735     339      87
 
NarI GG'CG_CC

Cuts at:      0    1446    1751    2247 
   Size:       1446     305     496

  Fragments arranged by size: 

                1446     496     305
 
NdeI CA'TA_TG

Cuts at:      0      55    2247 
   Size:         55    2192
 
NgoMIV G'CCGG_C

Cuts at:      0     337    1072    1159    2247 
   Size:        337     735      87    1088

  Fragments arranged by size: 

                1088     735     337      87
 
NspI r_CATG'y

Cuts at:      0     948    1942    2208    2247 
   Size:        948     994     266      39

  Fragments arranged by size: 

                 994     948     266      39
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     264    1152    2247 
   Size:        264     888    1095

  Fragments arranged by size: 

                1095     888     264
 
PfoI T'CCnGG_A

Cuts at:      0     641     797    1304    1607    2198    2247 
   Size:        641     156     507     303     591      49

  Fragments arranged by size: 

                 641     591     507     303     156      49
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     976    1008    2247 
   Size:        976      32    1239

  Fragments arranged by size: 

                1239     976      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     483     687     747     894    1199    1685    2247 
   Size:        483     204      60     147     305     486     562

  Fragments arranged by size: 

                 562     486     483     305     204     147      60
 
PspOMI G'GGCC_C

Cuts at:      0     386     566    2247 
   Size:        386     180    1681

  Fragments arranged by size: 

                1681     386     180
 
PstI C_TGCA'G

Cuts at:      0     395    1363    1788    2247 
   Size:        395     968     425     459

  Fragments arranged by size: 

                 968     459     425     395
 
PvuII CAG'CTG

Cuts at:      0     366    2231    2247 
   Size:        366    1865      16

  Fragments arranged by size: 

                1865     366      16
 
SacI G_AGCT'C

Cuts at:      0    1565    2108    2156    2247 
   Size:       1565     543      48      91

  Fragments arranged by size: 

                1565     543      91      48
 
SanDI GG'GwC_CC

Cuts at:      0    1685    2247 
   Size:       1685     562
 
SapI GCTCTTCn'nnn_

Cuts at:      0     959    1240    2247 
   Size:        959     281    1007

  Fragments arranged by size: 

                1007     959     281
 
ScaI AGT'ACT

Cuts at:      0    1630    2247 
   Size:       1630     617
 
SfcI C'TryA_G

Cuts at:      0     391    1359    1383    1784    2247 
   Size:        391     968      24     401     463

  Fragments arranged by size: 

                 968     463     401     391      24
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1705    2247 
   Size:       1705     542
 
SfoI GGC'GCC

Cuts at:      0    1447    1752    2247 
   Size:       1447     305     495

  Fragments arranged by size: 

                1447     495     305
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1442    2247 
   Size:       1442     805
 
SmaI CCC'GGG

Cuts at:      0     919    2081    2247 
   Size:        919    1162     166

  Fragments arranged by size: 

                1162     919     166
 
SmlI C'TyrA_G

Cuts at:      0     955    1261    1702    2247 
   Size:        955     306     441     545

  Fragments arranged by size: 

                 955     545     441     306
 
SphI G_CATG'C

Cuts at:      0     948    1942    2247 
   Size:        948     994     305

  Fragments arranged by size: 

                 994     948     305
 
SrfI GCCC'GGGC

Cuts at:      0    2081    2247 
   Size:       2081     166
 
StyI C'CwwG_G

Cuts at:      0    1454    2247 
   Size:       1454     793
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     265     715    1542    2247 
   Size:        265     450     827     705

  Fragments arranged by size: 

                 827     705     450     265
 
TatI w'GTAC_w

Cuts at:      0      80     146    1628    2247 
   Size:         80      66    1482     619

  Fragments arranged by size: 

                1482     619      80      66
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1319    1351    2247 
   Size:       1319      32     896

  Fragments arranged by size: 

                1319     896      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1320    1461    2247 
   Size:       1320     141     786

  Fragments arranged by size: 

                1320     786     141
 
XmaI C'CCGG_G

Cuts at:      0     917    2079    2247 
   Size:        917    1162     168

  Fragments arranged by size: 

                1162     917     168
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI    AflII     AleI     AlfI     AloI    AlwNI
     ApaI    ApaLI     AvaI     BanI    BanII     BbeI     BcgI     BclI
     BglI    BglII Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII     DrdI
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FspI
    HaeII    Hin4I     KasI     KpnI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NspI    PflMI     PfoI     PpiI
    PpuMI   PspOMI     PstI    PvuII     SacI    SanDI     SapI     ScaI
     SfcI     SfiI     SfoI    SgrAI     SmaI     SmlI     SphI     SrfI
     StyI    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI   AflIII     AgeI     AhdI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI
    BciVI    BfrBI     BlpI    BmgBI     BmtI   Bpu10I    BsaBI    BsaWI
    BsiEI    BsiWI     BsmI    BspEI    BspHI   BssHII   BstEII  BstZ17I
   Bsu36I     ClaI    CspCI     DraI     EagI     EciI    EcoRI     FalI
     FseI    FspAI   HincII  HindIII     HpaI     MfeI     MluI     NcoI
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI
     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI     SbfI    SexAI     SgfI    SnaBI     SpeI     SspI     StuI
     SwaI  Tth111I     XbaI     XhoI     XmnI     ZraI
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