(Linear) (Six-base) MAPSORT of: FLJ00006.seq Check: 7233 from: 1 to: 2247 FLJ00006.orf With 182 enzymes: SgfI * March 3, 2008 11:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 27 1983 2247 Size: 27 1956 264 Fragments arranged by size: 1956 264 27 Acc65I G'GTAC_C Cuts at: 0 2051 2247 Size: 2051 196 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 693 1747 1833 2115 2206 2211 2247 Size: 693 1054 86 282 91 5 36 Fragments arranged by size: 1054 693 282 91 86 36 5 AflII C'TTAA_G Cuts at: 0 1702 2247 Size: 1702 545 AleI CACnn'nnGTG Cuts at: 0 366 1087 1525 2247 Size: 366 721 438 722 Fragments arranged by size: 722 721 438 366 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 556 736 2247 Size: 556 180 1511 Fragments arranged by size: 1511 556 180 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 976 1008 2247 Size: 976 32 1239 Fragments arranged by size: 1239 976 32 AlwNI CAG_nnn'CTG Cuts at: 0 222 774 1152 1998 2184 2247 Size: 222 552 378 846 186 63 Fragments arranged by size: 846 552 378 222 186 63 ApaI G_GGCC'C Cuts at: 0 390 570 2247 Size: 390 180 1677 Fragments arranged by size: 1677 390 180 ApaLI G'TGCA_C Cuts at: 0 1341 2247 Size: 1341 906 AvaI C'yCGr_G Cuts at: 0 917 2079 2247 Size: 917 1162 168 Fragments arranged by size: 1162 917 168 BanI G'GyrC_C Cuts at: 0 540 607 1162 1445 1750 2051 2247 Size: 540 67 555 283 305 301 196 Fragments arranged by size: 555 540 305 301 283 196 67 BanII G_rGCy'C Cuts at: 0 390 570 916 1565 1985 2108 2156 2247 Size: 390 180 346 649 420 123 48 91 Fragments arranged by size: 649 420 390 346 180 123 91 48 BbeI G_GCGC'C Cuts at: 0 1449 1754 2247 Size: 1449 305 493 Fragments arranged by size: 1449 493 305 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1765 1799 2099 2133 2247 Size: 1765 34 300 34 114 Fragments arranged by size: 1765 300 114 34 34 BclI T'GATC_A Cuts at: 0 824 2247 Size: 824 1423 BglI GCCn_nnn'nGGC Cuts at: 0 1577 1705 2247 Size: 1577 128 542 Fragments arranged by size: 1577 542 128 BglII A'GATC_T Cuts at: 0 68 2247 Size: 68 2179 Bme1580I G_kGCm'C Cuts at: 0 390 545 570 610 1345 2247 Size: 390 155 25 40 735 902 Fragments arranged by size: 902 735 390 155 40 25 BmrI ACTGGGnnnn_n' Cuts at: 0 2157 2247 Size: 2157 90 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 189 1705 2247 Size: 189 1516 542 Fragments arranged by size: 1516 542 189 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 159 262 664 781 1273 1361 2010 2032 Size: 159 103 402 117 492 88 649 22 Cuts at: 2032 2247 Size: 215 Fragments arranged by size: 649 492 402 215 159 117 103 88 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 940 1246 2247 Size: 940 306 1001 Fragments arranged by size: 1001 940 306 BsaI GGTCTCn'nnnn_ Cuts at: 0 448 2247 Size: 448 1799 BsaAI yAC'GTr Cuts at: 0 1422 2247 Size: 1422 825 BsaHI Gr'CG_yC Cuts at: 0 656 1446 1751 2195 2247 Size: 656 790 305 444 52 Fragments arranged by size: 790 656 444 305 52 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 898 928 976 1006 1798 1828 2035 2065 Size: 898 30 48 30 792 30 207 30 Cuts at: 2065 2247 Size: 182 Fragments arranged by size: 898 792 207 182 48 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 661 664 1792 1900 2247 Size: 661 3 1128 108 347 Fragments arranged by size: 1128 661 347 108 3 BseYI C'CCAG_C Cuts at: 0 502 723 939 1202 1494 1550 2084 2231 Size: 502 221 216 263 292 56 534 147 Cuts at: 2231 2247 Size: 16 Fragments arranged by size: 534 502 292 263 221 216 147 56 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 817 934 1927 1984 2247 Size: 817 117 993 57 263 Fragments arranged by size: 993 817 263 117 57 BsiHKAI G_wGCw'C Cuts at: 0 563 1345 1432 1547 1565 1838 2021 2108 Size: 563 782 87 115 18 273 183 87 Cuts at: 2108 2156 2247 Size: 48 91 Fragments arranged by size: 782 563 273 183 115 91 87 87 48 18 BsmBI CGTCTCn'nnnn_ Cuts at: 0 300 2247 Size: 300 1947 Bsp1286I G_dGCh'C Cuts at: 0 390 545 563 570 610 916 1345 1432 Size: 390 155 18 7 40 306 429 87 Cuts at: 1432 1547 1565 1838 1985 2021 2108 2156 2247 Size: 115 18 273 147 36 87 48 91 Fragments arranged by size: 429 390 306 273 155 147 115 91 87 87 48 40 36 18 18 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 27 494 698 782 1983 2247 Size: 27 467 204 84 1201 264 Fragments arranged by size: 1201 467 264 204 84 27 BsrBI CCG'CTC Cuts at: 0 308 323 1476 1964 2247 Size: 308 15 1153 488 283 Fragments arranged by size: 1153 488 308 283 15 BsrDI GCAATG_nn' Cuts at: 0 1130 2247 Size: 1130 1117 BsrFI r'CCGG_y Cuts at: 0 337 610 1072 1159 1442 2247 Size: 337 273 462 87 283 805 Fragments arranged by size: 805 462 337 283 273 87 BsrGI T'GTAC_A Cuts at: 0 146 2247 Size: 146 2101 BssSI C'ACGA_G Cuts at: 0 1411 1675 2247 Size: 1411 264 572 Fragments arranged by size: 1411 572 264 BstAPI GCAn_nnn'nTGC Cuts at: 0 512 1939 1998 2247 Size: 512 1427 59 249 Fragments arranged by size: 1427 512 249 59 BstBI TT'CG_AA Cuts at: 0 1715 2247 Size: 1715 532 BstXI CCAn_nnnn'nTGG Cuts at: 0 183 368 723 864 1068 1599 2247 Size: 183 185 355 141 204 531 648 Fragments arranged by size: 648 531 355 204 185 183 141 BstYI r'GATC_y Cuts at: 0 68 270 2247 Size: 68 202 1977 Fragments arranged by size: 1977 202 68 BtgI C'CryG_G Cuts at: 0 1094 2039 2247 Size: 1094 945 208 Fragments arranged by size: 1094 945 208 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 444 1620 2247 Size: 444 1176 627 Fragments arranged by size: 1176 627 444 BtsI GCAGTG_nn' Cuts at: 0 829 1223 1545 1940 2247 Size: 829 394 322 395 307 Fragments arranged by size: 829 395 394 322 307 DraIII CAC_nnn'GTG Cuts at: 0 264 2247 Size: 264 1983 DrdI GACnn_nn'nnGTC Cuts at: 0 745 2247 Size: 745 1502 EaeI y'GGCC_r Cuts at: 0 74 335 620 1058 1070 1091 2247 Size: 74 261 285 438 12 21 1156 Fragments arranged by size: 1156 438 285 261 74 21 12 EarI CTCTTCn'nnn_ Cuts at: 0 89 394 959 1240 2158 2230 2247 Size: 89 305 565 281 918 72 17 Fragments arranged by size: 918 565 305 281 89 72 17 EcoICRI GAG'CTC Cuts at: 0 1563 2106 2154 2247 Size: 1563 543 48 93 Fragments arranged by size: 1563 543 93 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 159 262 664 693 781 1273 1361 1747 Size: 159 103 402 29 88 492 88 386 Cuts at: 1747 1833 2010 2032 2115 2206 2211 2247 Size: 86 177 22 83 91 5 36 Fragments arranged by size: 492 402 386 177 159 103 91 88 88 86 83 36 29 22 5 EcoNI CCTnn'n_nnAGG Cuts at: 0 646 958 1180 2140 2188 2247 Size: 646 312 222 960 48 59 Fragments arranged by size: 960 646 312 222 59 48 EcoO109I rG'GnC_Cy Cuts at: 0 386 387 483 567 687 747 894 1199 Size: 386 1 96 84 120 60 147 305 Cuts at: 1199 1458 1685 1707 2247 Size: 259 227 22 540 Fragments arranged by size: 540 386 305 259 227 147 120 96 84 60 22 1 EcoRV GAT'ATC Cuts at: 0 1017 2247 Size: 1017 1230 FspI TGC'GCA Cuts at: 0 553 886 2074 2247 Size: 553 333 1188 173 Fragments arranged by size: 1188 553 333 173 HaeII r_GCGC'y Cuts at: 0 1110 1449 1754 2247 Size: 1110 339 305 493 Fragments arranged by size: 1110 493 339 305 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1006 1038 1798 1830 2247 Size: 1006 32 760 32 417 Fragments arranged by size: 1006 760 417 32 32 KasI G'GCGC_C Cuts at: 0 1445 1750 2247 Size: 1445 305 497 Fragments arranged by size: 1445 497 305 KpnI G_GTAC'C Cuts at: 0 2055 2247 Size: 2055 192 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 470 1835 2247 Size: 470 1365 412 Fragments arranged by size: 1365 470 412 MscI TGG'CCA Cuts at: 0 76 1060 2247 Size: 76 984 1187 Fragments arranged by size: 1187 984 76 MslI CAynn'nnrTG Cuts at: 0 366 538 613 1087 1525 2209 2247 Size: 366 172 75 474 438 684 38 Fragments arranged by size: 684 474 438 366 172 75 38 MspA1I CmG'CkG Cuts at: 0 366 744 1206 1404 2231 2247 Size: 366 378 462 198 827 16 Fragments arranged by size: 827 462 378 366 198 16 NaeI GCC'GGC Cuts at: 0 339 1074 1161 2247 Size: 339 735 87 1086 Fragments arranged by size: 1086 735 339 87 NarI GG'CG_CC Cuts at: 0 1446 1751 2247 Size: 1446 305 496 Fragments arranged by size: 1446 496 305 NdeI CA'TA_TG Cuts at: 0 55 2247 Size: 55 2192 NgoMIV G'CCGG_C Cuts at: 0 337 1072 1159 2247 Size: 337 735 87 1088 Fragments arranged by size: 1088 735 337 87 NspI r_CATG'y Cuts at: 0 948 1942 2208 2247 Size: 948 994 266 39 Fragments arranged by size: 994 948 266 39 PflMI CCAn_nnn'nTGG Cuts at: 0 264 1152 2247 Size: 264 888 1095 Fragments arranged by size: 1095 888 264 PfoI T'CCnGG_A Cuts at: 0 641 797 1304 1607 2198 2247 Size: 641 156 507 303 591 49 Fragments arranged by size: 641 591 507 303 156 49 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 976 1008 2247 Size: 976 32 1239 Fragments arranged by size: 1239 976 32 PpuMI rG'GwC_Cy Cuts at: 0 483 687 747 894 1199 1685 2247 Size: 483 204 60 147 305 486 562 Fragments arranged by size: 562 486 483 305 204 147 60 PspOMI G'GGCC_C Cuts at: 0 386 566 2247 Size: 386 180 1681 Fragments arranged by size: 1681 386 180 PstI C_TGCA'G Cuts at: 0 395 1363 1788 2247 Size: 395 968 425 459 Fragments arranged by size: 968 459 425 395 PvuII CAG'CTG Cuts at: 0 366 2231 2247 Size: 366 1865 16 Fragments arranged by size: 1865 366 16 SacI G_AGCT'C Cuts at: 0 1565 2108 2156 2247 Size: 1565 543 48 91 Fragments arranged by size: 1565 543 91 48 SanDI GG'GwC_CC Cuts at: 0 1685 2247 Size: 1685 562 SapI GCTCTTCn'nnn_ Cuts at: 0 959 1240 2247 Size: 959 281 1007 Fragments arranged by size: 1007 959 281 ScaI AGT'ACT Cuts at: 0 1630 2247 Size: 1630 617 SfcI C'TryA_G Cuts at: 0 391 1359 1383 1784 2247 Size: 391 968 24 401 463 Fragments arranged by size: 968 463 401 391 24 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1705 2247 Size: 1705 542 SfoI GGC'GCC Cuts at: 0 1447 1752 2247 Size: 1447 305 495 Fragments arranged by size: 1447 495 305 SgrAI Cr'CCGG_yG Cuts at: 0 1442 2247 Size: 1442 805 SmaI CCC'GGG Cuts at: 0 919 2081 2247 Size: 919 1162 166 Fragments arranged by size: 1162 919 166 SmlI C'TyrA_G Cuts at: 0 955 1261 1702 2247 Size: 955 306 441 545 Fragments arranged by size: 955 545 441 306 SphI G_CATG'C Cuts at: 0 948 1942 2247 Size: 948 994 305 Fragments arranged by size: 994 948 305 SrfI GCCC'GGGC Cuts at: 0 2081 2247 Size: 2081 166 StyI C'CwwG_G Cuts at: 0 1454 2247 Size: 1454 793 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 265 715 1542 2247 Size: 265 450 827 705 Fragments arranged by size: 827 705 450 265 TatI w'GTAC_w Cuts at: 0 80 146 1628 2247 Size: 80 66 1482 619 Fragments arranged by size: 1482 619 80 66 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1319 1351 2247 Size: 1319 32 896 Fragments arranged by size: 1319 896 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1320 1461 2247 Size: 1320 141 786 Fragments arranged by size: 1320 786 141 XmaI C'CCGG_G Cuts at: 0 917 2079 2247 Size: 917 1162 168 Fragments arranged by size: 1162 917 168 Enzymes that do cut: AarI Acc65I AcuI AflII AleI AlfI AloI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BcgI BclI BglI BglII Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SanDI SapI ScaI SfcI SfiI SfoI SgrAI SmaI SmlI SphI SrfI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AatII AccI AclI AfeI AflIII AgeI AhdI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BfrBI BlpI BmgBI BmtI Bpu10I BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraI EagI EciI EcoRI FalI FseI FspAI HincII HindIII HpaI MfeI MluI NcoI NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI SexAI SgfI SnaBI SpeI SspI StuI SwaI Tth111I XbaI XhoI XmnI ZraI