MKL2 ORF clone FHC13131

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC13131

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Clone info.

Species

human

Clone Name

pFN21AE5560 Sequence (with vector) Restriction Map

Product ID

FHC13131

ORF Sequence

DNA sequence (1134 bp)
Protein sequence (378 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5560

Symbol

MKL2

Description

MKL/myocardin-like 2

Original Clone ID

oc95560

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1134 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 378 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS32391.1 1.6e-58 99.7 MKL2 CCDS76823.1 1.7e-58 99.7 MKL2 CCDS82720.1 3.5e-36 68.1 MKL1 CCDS74866.1 5.1e-33 67.3 MKL1 CCDS14003.1 5.8e-33 67.3 MKL1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR004018 2 23 PF02755 RPEL repeat IPR004018 44 68 PF02755 RPEL repeat IPR004018 88 112 PF02755 RPEL repeat HMMSmart IPR004018 2 25 SM00707 RPEL repeat IPR004018 44 69 SM00707 RPEL repeat IPR004018 88 113 SM00707 RPEL repeat ProfileScan IPR004018 1 25 PS51073 RPEL repeat IPR004018 44 69 PS51073 RPEL repeat IPR004018 88 113 PS51073 RPEL repeat
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_016878994 1020 609463 99.7 81.7 XP_016878993 1038 609463 99.7 81.7 NP_054767 1049 609463 99.7 81.7 XP_006720977 1049 609463 99.7 81.7 XP_006720976 1053 609463 99.7 81.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5560 Download

>KIEE5560  1134 bp
ATGCTCCAGCTGAGGCTGCAACAAAGGAGGACGAGAGAACAACTAGTGGACCAGGGCATC
ATGCCACCTTTGAAGAGCCCAGCGGCATTCCATGAACAGATAAAAAGCTTGGAACGAGCC
AGAACTGAAAACTTTTTGAAACACAAGATTCGGAGTCGACCAGATCGTTCTGAACTTGTC
AGGATGCACATTTTAGAAGAAACATTTGCAGAGCCATCCCTGCAGGCTACTCAGATGAAG
TTGAAAAGAGCTCGACTAGCAGATGATCTGAATGAAAAGATTGCTCAAAGACCTGGTCCT
ATGGAGCTGGTAGAGAAAAACATCCTTCCTGTGGACTCCAGTGTTAAAGAAGCAATTATA
GGCGTTGGGAAGGAGGACTATCCCCACACTCAGGGCGATTTCTCATTTGATGAAGACAGC
AGTGACGCTTTGTCTCCGGACCAGCCTGCGAGTCAGGAGTCACAGGGGTCAGCCGCGTCC
CCAAGTGAGCCAAAAGTTAGTGAATCGCCATCTCCTGTGACTACAAACACTCCAGCGCAG
TTTGCTTCAGTGTCCCCAACAGTTCCTGAATTCTTGAAAACTCCTCCAACTGCAGATCAG
CCTCCCCCACGGCCTGCAGCTCCTGTCCTCCCCACAAACACTGTGTCCTCAGCAAAGCCT
GGCCCAGCACTGGTGAAGCAAAGCCATCCCAAGAATCCAAATGACAAACACCGTAGCAAA
AAGTGCAAAGATCCCAAACCACGGGTAAAGAAGTTAAAGTACCACCAATACATTCCACCA
GATCAGAAGGGTGAGAAGAATGAGCCGCAGATGGACTCTAACTACGCCCGCCTGCTCCAG
CAGCAGCAGCTGTTCCTGCAACTGCAGATCCTGAGTCAGCAGAAGCAGCACTACAACTAC
CAGACCATCCTGCCTGCACCATTCAAAGCTTATCACACAGTGTCTGAAGTCCACATGGTG
AGAGTGGCCTGCATTCCATTCCAGTTTCTGTCCTCCAAAATAGGTTCTGAATTTTTACAA
GTTAGAAATGCCTTTTCACAATTATTCATCCAGATTTGCCTATTATTAGAACACCAAAAT
AGTACTAGATGCTCTGAGAAAAGCGTAAGTAGTATAATACCTGGCATAAACAGC

ORF Protein

Cloned ORF protein sequence for pFN21AE5560 Download

>KIEE5560  378 aa
MLQLRLQQRRTREQLVDQGIMPPLKSPAAFHEQIKSLERARTENFLKHKIRSRPDRSELV
RMHILEETFAEPSLQATQMKLKRARLADDLNEKIAQRPGPMELVEKNILPVDSSVKEAII
GVGKEDYPHTQGDFSFDEDSSDALSPDQPASQESQGSAASPSEPKVSESPSPVTTNTPAQ
FASVSPTVPEFLKTPPTADQPPPRPAAPVLPTNTVSSAKPGPALVKQSHPKNPNDKHRSK
KCKDPKPRVKKLKYHQYIPPDQKGEKNEPQMDSNYARLLQQQQLFLQLQILSQQKQHYNY
QTILPAPFKAYHTVSEVHMVRVACIPFQFLSSKIGSEFLQVRNAFSQLFIQICLLLEHQN
STRCSEKSVSSIIPGINS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5560 Download

>pFN21AE5560  5839 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCTCCAGCTGAGGCTGCAACAAAGGAGGACGAG
AGAACAACTAGTGGACCAGGGCATCATGCCACCTTTGAAGAGCCCAGCGGCATTCCATGA
ACAGATAAAAAGCTTGGAACGAGCCAGAACTGAAAACTTTTTGAAACACAAGATTCGGAG
TCGACCAGATCGTTCTGAACTTGTCAGGATGCACATTTTAGAAGAAACATTTGCAGAGCC
ATCCCTGCAGGCTACTCAGATGAAGTTGAAAAGAGCTCGACTAGCAGATGATCTGAATGA
AAAGATTGCTCAAAGACCTGGTCCTATGGAGCTGGTAGAGAAAAACATCCTTCCTGTGGA
CTCCAGTGTTAAAGAAGCAATTATAGGCGTTGGGAAGGAGGACTATCCCCACACTCAGGG
CGATTTCTCATTTGATGAAGACAGCAGTGACGCTTTGTCTCCGGACCAGCCTGCGAGTCA
GGAGTCACAGGGGTCAGCCGCGTCCCCAAGTGAGCCAAAAGTTAGTGAATCGCCATCTCC
TGTGACTACAAACACTCCAGCGCAGTTTGCTTCAGTGTCCCCAACAGTTCCTGAATTCTT
GAAAACTCCTCCAACTGCAGATCAGCCTCCCCCACGGCCTGCAGCTCCTGTCCTCCCCAC
AAACACTGTGTCCTCAGCAAAGCCTGGCCCAGCACTGGTGAAGCAAAGCCATCCCAAGAA
TCCAAATGACAAACACCGTAGCAAAAAGTGCAAAGATCCCAAACCACGGGTAAAGAAGTT
AAAGTACCACCAATACATTCCACCAGATCAGAAGGGTGAGAAGAATGAGCCGCAGATGGA
CTCTAACTACGCCCGCCTGCTCCAGCAGCAGCAGCTGTTCCTGCAACTGCAGATCCTGAG
TCAGCAGAAGCAGCACTACAACTACCAGACCATCCTGCCTGCACCATTCAAAGCTTATCA
CACAGTGTCTGAAGTCCACATGGTGAGAGTGGCCTGCATTCCATTCCAGTTTCTGTCCTC
CAAAATAGGTTCTGAATTTTTACAAGTTAGAAATGCCTTTTCACAATTATTCATCCAGAT
TTGCCTATTATTAGAACACCAAAATAGTACTAGATGCTCTGAGAAAAGCGTAAGTAGTAT
AATACCTGGCATAAACAGCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT