NPTN ORF clone FHC12389

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC12389

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Clone info.

Species

human

Clone Name

pFN21AE6642 Sequence (with vector) Restriction Map

Product ID

FHC12389

ORF Sequence

DNA sequence (846 bp)
Protein sequence (282 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6642

Symbol

NPTN

Alias : GP55, GP65, SDFR1, SDR1, np55, np65

Description

neuroplastin, transcript variant a

Original Clone ID

oc96642

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 846 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 282 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10250.1 3.5e-98 100.0 NPTN CCDS58379.1 2.1e-95 98.6 NPTN CCDS10249.1 1.2e-87 99.6 NPTN CCDS58380.1 7.3e-85 98.0 NPTN CCDS12034.1 5.2e-29 38.4 BSG The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR009151 2 15 PR01856 Basigin IPR009151 110 128 PR01856 Basigin IPR009151 132 143 PR01856 Basigin IPR009151 173 194 PR01856 Basigin HMMPfam IPR013098 122 217 PF07679 Immunoglobulin I-set HMMSmart IPR003599 42 119 SM00409 Immunoglobulin subtype IPR003599 128 218 SM00409 Immunoglobulin subtype IPR003598 134 207 SM00408 Immunoglobulin subtype 2 ProfileScan IPR007110 32 119 PS50835 Immunoglobulin-like IPR007110 122 213 PS50835 Immunoglobulin-like
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 6 LPSALALSLLLVSGSLLPGPGA 27 SECONDARY 22 2 222 APLWPFLGILAEIIILVVIIVV 243 PRIMARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_059429 282 612820 100.0 100.0 NP_001154836 278 612820 98.6 100.0 NP_036560 398 612820 99.6 89.4 NP_001154835 394 612820 98.0 89.4 XP_011519754 372 612820 99.6 80.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6642 Download

>KIEE6642  846 bp
ATGTCGGGTTCGTCGCTGCCCAGCGCCCTGGCCCTCTCGCTGTTGCTGGTCTCTGGCTCC
CTCCTCCCAGGGCCAGGCGCCGCTCAGAACGAGCCAAGGATTGTCACCAGTGAAGAGGTC
ATTATTCGAGACAGCCCTGTTCTCCCTGTCACCCTGCAGTGTAACCTCACCTCCAGCTCT
CACACCCTTACATACAGCTACTGGACAAAGAATGGGGTGGAACTGAGTGCCACTCGTAAG
AATGCCAGCAACATGGAGTACAGGATCAATAAGCCGAGAGCTGAGGATTCAGGCGAATAC
CACTGCGTATATCACTTTGTCAGCGCTCCTAAAGCAAACGCCACCATTGAAGTGAAAGCC
GCTCCTGACATCACTGGCCATAAACGGAGTGAGAACAAGAATGAAGGGCAGGATGCCACT
ATGTATTGCAAGTCAGTTGGCTACCCCCACCCAGACTGGATATGGCGCAAGAAGGAGAAC
GGGATGCCCATGGACATTGTCAATACCTCTGGCCGCTTCTTCATCATCAACAAGGAAAAT
TACACTGAGTTGAACATTGTGAACCTGCAGATCACGGAAGACCCTGGCGAGTATGAATGT
AATGCCACCAACGCCATTGGCTCCGCCTCTGTTGTCACTGTCCTCAGGGTGCGGAGCCAC
CTGGCCCCACTCTGGCCTTTCTTGGGAATTCTGGCTGAAATTATCATCCTTGTGGTGATC
ATTGTTGTGTATGAGAAGAGGAAGAGGCCAGATGAGGTTCCTGACGATGATGAACCAGCT
GGACCAATGAAAACCAACTCTACCAACAATCACAAAGATAAAAACTTGCGCCAGAGAAAC
ACAAAT

ORF Protein

Cloned ORF protein sequence for pFN21AE6642 Download

>KIEE6642  282 aa
MSGSSLPSALALSLLLVSGSLLPGPGAAQNEPRIVTSEEVIIRDSPVLPVTLQCNLTSSS
HTLTYSYWTKNGVELSATRKNASNMEYRINKPRAEDSGEYHCVYHFVSAPKANATIEVKA
APDITGHKRSENKNEGQDATMYCKSVGYPHPDWIWRKKENGMPMDIVNTSGRFFIINKEN
YTELNIVNLQITEDPGEYECNATNAIGSASVVTVLRVRSHLAPLWPFLGILAEIIILVVI
IVVYEKRKRPDEVPDDDEPAGPMKTNSTNNHKDKNLRQRNTN

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6642 Download

>pFN21AE6642  5551 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCGGGTTCGTCGCTGCCCAGCGCCCTGGCCCT
CTCGCTGTTGCTGGTCTCTGGCTCCCTCCTCCCAGGGCCAGGCGCCGCTCAGAACGAGCC
AAGGATTGTCACCAGTGAAGAGGTCATTATTCGAGACAGCCCTGTTCTCCCTGTCACCCT
GCAGTGTAACCTCACCTCCAGCTCTCACACCCTTACATACAGCTACTGGACAAAGAATGG
GGTGGAACTGAGTGCCACTCGTAAGAATGCCAGCAACATGGAGTACAGGATCAATAAGCC
GAGAGCTGAGGATTCAGGCGAATACCACTGCGTATATCACTTTGTCAGCGCTCCTAAAGC
AAACGCCACCATTGAAGTGAAAGCCGCTCCTGACATCACTGGCCATAAACGGAGTGAGAA
CAAGAATGAAGGGCAGGATGCCACTATGTATTGCAAGTCAGTTGGCTACCCCCACCCAGA
CTGGATATGGCGCAAGAAGGAGAACGGGATGCCCATGGACATTGTCAATACCTCTGGCCG
CTTCTTCATCATCAACAAGGAAAATTACACTGAGTTGAACATTGTGAACCTGCAGATCAC
GGAAGACCCTGGCGAGTATGAATGTAATGCCACCAACGCCATTGGCTCCGCCTCTGTTGT
CACTGTCCTCAGGGTGCGGAGCCACCTGGCCCCACTCTGGCCTTTCTTGGGAATTCTGGC
TGAAATTATCATCCTTGTGGTGATCATTGTTGTGTATGAGAAGAGGAAGAGGCCAGATGA
GGTTCCTGACGATGATGAACCAGCTGGACCAATGAAAACCAACTCTACCAACAATCACAA
AGATAAAAACTTGCGCCAGAGAAACACAAATGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT